Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 3819066 | Connective tissue diseases and bovine collagen implants. | 1987 Feb | Bovine collagen implants are biomaterial used for the correction of dermal contour deformities. The use of bovine collagen implants in patients with a personal history of autoimmune diseases is contraindicated by the manufacturer. In our study, sera from fifty patients with scleroderma, rheumatoid arthritis, and systemic lupus erythematosus were examined for antibodies to bovine collagen implants by means of a previously described enzyme-linked immunosorbent assay. None of the patients had received bovine collagen implants. The anti-bovine collagen implant antibody levels in the sera of patients with scleroderma, systemic lupus erythematosus, and rheumatoid arthritis were not, in general, statistically different from those in the normal population. | |
| 2430586 | Low density neutrophils in patients with systemic lupus erythematosus, rheumatoid arthriti | 1986 Nov | Ficoll-Hypaque density gradient preparations of peripheral blood mononuclear cells from patients with systemic lupus erythematosus, rheumatoid arthritis, and acute rheumatic fever were highly "contaminated" with low buoyant density neutrophils. Plasma from these patients could induce an in vitro decrease of buoyancy in neutrophils with normal buoyant density. Similar change could be induced by complement-activated sera and aggregated gamma globulin. These data suggest that activated neutrophils are a common finding in the peripheral blood of these patients and may influence the interpretation of any studies with these cells. Functional studies of lymphocytes separated by Ficoll-Hypaque gradients should also take into account the higher degree of impurity of the cell preparations in patients with rheumatic diseases. | |
| 3207372 | Humoral immunity to link protein in patients with inflammatory joint disease, osteoarthrit | 1988 Nov | Cartilage link protein of high purity was prepared and used in an enzyme linked immunosorbent assay (ELISA). Antibodies to link protein were sought in the sera of 98 patients with rheumatic disorders; 38 with rheumatoid arthritis (RA), 29 with osteoarthritis (OA), 13 with psoriatic arthritis (PA), nine with ankylosing spondylitis (AS), nine with systemic lupus erythematosus (SLE), and in 83 healthy controls. Antibodies were detected in all groups with the following prevalences: 21/83 normals, 9/38 RA, 7/29 OA, 7/13 PA, 3/9 AS, and 4/9 SLE. No statistically significant differences existed between the groups with regard to either prevalence or mean titre of anti-link antibodies. Serum antibodies to proteoglycan link protein appear to be no more common in patients with rheumatic disorders than in healthy controls. | |
| 3146623 | Spontaneous temporomandibular joint arthropathy in MRL-lpr/lpr mice. | 1988 Sep | The temporomandibular joint (TMJ) in spontaneously arthritic MRL lpr/lpr (MRL/l) mice has been histologically analysed. After decalcification and standardized preparation of the joint tissue, histological sections were examined for presence of synovial hypertrophy, synovial villi, pannus tissue, erosions, cysts and signs of periarticular inflammation. In 12 of 13 TMJs examined, histopathological changes indicating a state of arthropathy were found. The most prevalent finding was synovial proliferation, which was frequently detected at the insertion of the synovial membrane to the bone. Two or more signs of inflammatory involvement including synovial proliferation, pannus tissue and bone erosion were seen in 5 mice with a predominance among the oldest mice (greater than or equal to 5 months of age). No lymphocytic cell infiltration was found in the analyzed synovial tissues of the TMJ. In contrast, lymphocytes were observed in the vicinity of periarticular blood vessels and tendons. It is concluded that the TMJ is frequently involved in the arthritic disease of autoimmune MRL/l mice. | |
| 2242066 | Interleukin-1-induced interleukin-6 is required for the inhibition of proteoglycan synthes | 1990 Nov | Cartilage from normal controls, patients with osteoarthritis, and patients with rheumatoid arthritis produced no interleukin-6 (IL-6) in culture. However, IL-1 induced massive production of IL-6 (up to 135 ng/ml) in cartilage from all 3 sources, in a dose-dependent manner (in some cases, a peak value was reached). The levels of induced IL-6 were similar to those found in rheumatoid arthritis synovial fluid. At IL-1 concentrations that induced almost complete inhibition of proteoglycan (PG) synthesis, IL-6 production could still be increased considerably. Exogenous IL-6 inhibited PG synthesis by up to 25%. IL-1-induced inhibition of PG synthesis was reversed by antibodies against recombinant human IL-6. These results suggest that IL-6 is required for the IL-1-induced inhibition of PG synthesis. | |
| 2025307 | Diversity of rheumatoid synovial tissue T cells by T cell receptor analysis. Oligoclonal e | 1991 May | The synovitis of rheumatoid arthritis (RA) is characterized by infiltrates of CD4+ T lymphocytes. To determine the clonal diversity of these cells, we cloned T cells with interleukin-2 (IL-2), alone or with phytohemagglutinin (PHA), directly from actively inflamed synovial tissue obtained at synovectomy. A total of 205 clones from 4 specimens was analyzed for T cell receptor (TCR) gene rearrangements using Hind III and Eco RI digests with beta chain and gamma chain complementary DNA probes. A comparison of the TCR rearrangements enabled us to determine if the T cell clones arose from the same or different precursor cells. Most of the T cell clones (92%) had distinct TCR gene rearrangement patterns, indicating a unique clonal origin. However, a few clones (1 quadruplicate and 6 pairs) with identical TCR rearrangements were identified, and these clonal multiples were most commonly found in clones selected with IL-2 alone. Mass cultures were propagated with IL-2, alone or with PHA, and at each passage, cells were removed for TCR analysis. The later passages of the lines selected with IL-2 had oligoclonal TCR rearrangements, whereas no oligoclonal rearrangements were found in the PHA + IL-2-selected cell lines. The TCR rearrangements in the later passages of the IL-2 mass cultures were often identical to the TCR rearrangements that were found in the IL-2-derived clonal multiples. These findings indicate that while the majority of CD4+ T cells within the actively inflamed rheumatoid joint have diverse clonal origins, small numbers of clonal multiples and oligoclonal populations are present, and these cells may be enriched in an IL-2-responsive T cell subset. | |
| 3266992 | T-cell functional defects in rheumatoid arthritis: intrinsic or extrinsic? | 1988 Aug | This study investigated two mechanisms which may underlie abnormal T-cell function [lymphocyte proliferation and interleukin-2 (IL-2) production] in rheumatoid arthritis (RA). These were: (a) a possible lack of the IL-2-producing CD4+2H4+ lymphocytes and (b) the possible inhibitory role of monocytes and neutrophils. Numbers of CD4+2H4+ cells did not differ between normal controls and patients with RA, although IL-2 produced by the peripheral blood mononuclear cells (PBMC) of the same individuals was markedly reduced in the patient group (P less than 0.001). Many rheumatoid peripheral blood mononuclear cell preparations, but very few control, were contaminated with neutrophils (P less than 0.001). This was more marked in patients with active RA than in those with inactive disease (P less than 0.001). Numbers of monocytes were similar in all groups. Monocyte depletion, or addition of indomethacin and/or catalase in PBMC, caused a significantly greater increase of responses in RA patients than in controls. This effect was significantly higher in patients with active disease than in the inactive group. These findings suggest that activated monocytes and neutrophils found in the rheumatoid PBMC preparations exert inhibitory effects mediated, in part, by the production of prostaglandins and reactive oxygen intermediates. Monocyte depletion and partial reconstitution resulted in significant increase of lymphocyte proliferation and IL-2 production in both controls and patients. None of the manipulations performed succeeded in normalizing the deficient rheumatoid T-cell responses. These data support the hypothesis that non-lymphoid cell populations play an important role in the T-cell dysfunction characteristic of RA.(ABSTRACT TRUNCATED AT 250 WORDS) | |
| 2123137 | Probing of the rheumatoid factor (RF) V gene repertoire in rheumatoid arthritis (RA) by hy | 1990 Jul | Twenty human monoclonal antibodies with rheumatoid factor (RF) specificity were produced from fusions using B lymphocytes derived from the synovial tissue of two patients with rheumatoid arthritis (RA) and one with the polyarticular form of juvenile rheumatoid arthritis (JRA) (1). All the 20 monoclonal antibodies were IgM. Fourteen of these were classical RFs with specificity restricted to IgG, and included 12 kappa and 2 lambda proteins. When the fine specificity for IgG Fc determinants were investigated most of them showed the Ga specificity. In addition, 5 lambda and 1 kappa monoclonal RF antibodies showed polyreactivity and also reacted with various other antigens than IgG (1). The 14 monoreactive RFs were further studied for the expression of RF-related cross-reactive idiotypes (CRI) and variable heavy (VH) and light chain (VL) subgroups. Only four of the twelve kappa RFs expressed the V kappa III subgroup. Three of them belong to the V kappa IIIb sub-subgroup and expressed the CRI 17.109. One of these 3 clones in addition expressed the VH I associated CRI G6. Five other monoreactive RFs expressed either or both of the VH III associated CRI B6 and D12 (2). Using staphylococcal protein A (SPA) binding as well as Northern blotting techniques (2), studies indicated that 10 out of the 12 RFs studied expressed the VH III regions and 2 expressed the VH I region. These data, both for the heavy and light chains, indicated a different V gene usage by the RF derived from RA patients than by the RF M-components derived from patients with mixed cryoglobulinemia and Waldenström's macroglobulinemia but without RA.(ABSTRACT TRUNCATED AT 250 WORDS) | |
| 3100335 | Induction of cyclo-oxygenase by interleukin-1 in rheumatoid synovial cells. | 1987 Feb 9 | The ability of interleukin-1 (IL-1) to stimulate prostaglandin E2 (PGE2) production by human rheumatoid adherent synovial cells was found to be time-dependent and sensitive to protein synthesis inhibitors. Cells incubated with exogenous arachidonic acid (10 microM) showed no increase in PGE2 production. However, with IL-1 (2.5 U/ml) and exogenous arachidonic acid there was a marked increase, with levels reaching twice that for cells incubated with IL-1 alone. Aspirin pre-treatment studies and the use of [acetyl-14C]aspirin showed that IL-1 increased PGE2 production through the induction of cyclo-oxygenase. | |
| 3675011 | Experimental autoimmune arthritis in mice. I. Homologous type II collagen is responsible f | 1987 Sep | Immunisation with heterologous type II collagen (CII) induces arthritis in mice of the DBA/1 strain, which is genetically susceptible to this disease. To develop an experimental model of autoimmunity more adequate for the study of human rheumatoid arthritis (RA), DBA/1 mice were injected with 100 micrograms of native CII that had been purified from mouse xiphoid cartilage. About six weeks later the animals developed a chronic progressive polyarthritis involving the four paws but mainly confined to interphalangeal and metatarsophalangeal joints. The evolution of the disease fluctuated between remissions and exacerbations. The initial lesions assessed by clinical observations were more severe when the disease occurred early than in the case of late onset. Interestingly, the incidence of arthritis was clearly preponderant in males, and, moreover, the few female mice which developed arthritis had mild disease states with lower arthritic scores than the males. Varying levels of autoantibodies against mouse CII were found in the sera of immunised animals, regardless of the development of arthritis. These data indicate that the injection of homologous CII into mice caused a polyarthritis that is clinically closer to the human RA than the disease induced with heterologous CII and therefore will represent a useful tool for the study of the self-perpetuating mechanisms that characterise RA. | |
| 2505317 | Quantitative immunohistochemistry: standardization and possible application in research an | 1989 | We describe three different methods of calibration of quantitative immunohistochemistry, two of them based on self-made dye-agarose or antigen-agarose substrates. Quantification of immunostained objects (immunosignals) can be done by (1) microscope photometry (plug method) or scanning-photometry; (2) and densitometry of a television transformed microscope image. Both approaches are subjected to the different methods of calibration and three examples of application. | |
| 3085380 | [Fabry disease, a disease with rheumatic aspects: radiology of soft tissue and bone change | 1986 Jan | Fabry's disease is a hereditary lipid storage disease with deposition of ceramides in nearly all tissues. The lipid deposition in the skin and the synovial compartments causes an enlargement of the skin, the joint capsules and the tendon sheaths, which are recognizable by low KV radiography. The bones of the hand mainly show multiple enthesopathic ossifications of a peculiar kind at the insertions of fibrous structures and few intra- and extraarticular erosions. The clinical picture often simulates an inflammatory rheumatic disease. | |
| 3119129 | Repeat prescribing of non-steroidal anti-inflammatory drugs excluding aspirin: how careful | 1987 Oct 17 | About 5% of all National Health Service prescriptions in Britain and a quarter of reports of suspected adverse reactions are accounted for by non-steroidal anti-inflammatory drugs. Their prescription was investigated in two computerised group practices serving 11850 patients. Altogether 198 patients receiving repeat prescriptions of non-steroidal anti-inflammatory drugs were identified and relevant clinical details extracted from their notes. Of these patients, 119 were over 65 years old; 172 were receiving one of six different non-steroidal anti-inflammatory drugs; and 76 were taking drugs that can interact with non-steroidal anti-inflammatory drugs. Ninety one patients had one or more medical conditions that may be aggravated by non-steroidal anti-inflammatory drugs, and 36 had experienced side effects important enough for their treatment to be changed. A questionnaire to assess opinions and knowledge of non-steroidal anti-inflammatory drugs was given to 42 general practitioners and 26 rheumatologists. Although the two groups showed a comparable knowledge of the properties and costs of non-steroidal anti-inflammatory drugs, they differed significantly in their views on the circumstances under which these drugs should be used. Clear guidelines on the prescription of these drugs would indicate when careful monitoring is essential for patients to benefit from them safely. | |
| 1809765 | Long-term effect of intra-articular injections of a glucocorticosteroid into the TMJ: a cl | 1991 Winter | The long-term (8 years) effect of intra-articular injections of glucocorticosteroid into the TMJ on subjective and clinical dysfunction in the stomatognathic system and on the radiographic appearance of the TMJ was investigated in 16 patients with signs of TMJ arthritis. The subjective symptoms as well as the clinical signs were significantly reduced at the follow-up examination. Erosions of the bony articular margins of the TMJ that could be observed radiographically before treatment were found to be remineralized at the follow-up and associated with bony remodeling (deviation in shape) of the joint. The results indicate that the long-term prognosis of intra-articular glucocorticosteroid injections for TMJ arthritis is good and that there are no radiographically demonstrable side effects of the treatment. | |
| 2613795 | Determination of serum cytidine deaminase activity using ion-pair reversed-phase liquid ch | 1989 Oct 27 | A rapid and sensitive assay for serum cytidine deaminase has been developed utilising ion-pair reversed-phase high-performance liquid chromatography. The addition of 1-octanesulphonic acid (OSA) caused the retention of cytidine and uridine to reverse and uridine, the minor component in the assay, to elute first. Cytidine, uridine and allopurinol (internal standard) were separated on a 5-micron Hypersil ODS column using 100 mM ammonium acetate with 1% (v/v) methanol and 1 mM OSA adjusted to pH 5.0. Detection was at 262 nm. Peak areas were linear from 7 pmol to 6 nmol injected (r = 0.99). Intra-assay variation was 7.8% (n = 10) and the correlation with a colorimetric assay was r = 0.78 (p less than 0.001). | |
| 3115202 | Factor VIII related antigen in the assessment of vasculitis. | 1987 Jun | Factor VIII related antigen, an endothelial cell product, was markedly raised in systemic necrotising arteritis, reflecting disease activity, but was not raised in active cutaneous vasculitis. In rheumatoid arthritis high levels were only found in systemic vasculitis or Felty's syndrome, but in other connective tissue diseases increased levels were more frequently detected and usually related to disease activity. It did not correlate with C reactive protein. It was also raised in non-inflammatory peripheral vascular disease and after surgery and was not specific for vasculitic endothelial damage. Factor VIII related antigen may be useful in identifying and monitoring systemic necrotising and large vessel arteritis. | |
| 3276420 | Pelvic mass causing vesical compression after total hip arthroplasty. Case report. | 1988 Feb | A 59-year-old woman developed a histiocytic response to methylmethacrylate debris in a loose total hip arthroplasty. This reaction eroded through the medial wall of the acetabulum, producing a pelvic mass and presenting with urinary symptoms. A combination of an abdominal approach for removal of the pelvic mass and a lateral approach for revision of the total hip arthroplasty was required. This condition should be considered in the differential diagnosis of a mass adjacent to a loose prosthesis. | |
| 2609055 | [Clinical value of determining the N-acetyl-beta-D-glucosaminidase activity in joint disea | 1989 Jul | The study of the activity of N-acetyl-beta-D-glucosaminidase and its isoenzymes in the blood serum of patients with RA and healthy subjects showed that these figures were significantly higher in RA patients. Direct dependence was noted between the degree of the RA process activity presence of systemic lesions and synovitis. | |
| 1779159 | Long-term follow-up of the cemented Caffinière prosthesis for trapezio-metacarpal arthrop | 1991 Nov | 20 patients with 22 Caffinière prostheses in the trapezio-metacarpal joint were evaluated at a median of nine years after operation. The indication was degenerative osteoarthrosis in 20 cases and rheumatoid arthritis in two cases. We found 18 of the 22 (82%) original prostheses still in place, with satisfactory pain relief and good function. Three hands had been revised due to aseptic loosening, with replacement of two cups and one total prosthesis. Two of the revised prostheses were functioning well 6 1/2 and 10 years after the revision. We conclude that function does not deteriorate within the first nine years, that late loosening is not a significant problem with this prosthesis and that, if aseptic loosening occurs, it is possible to obtain good results by replacing the loose component. | |
| 2239788 | Catabolic effects of high-dose corticosteroids persist despite therapeutic benefit in rheu | 1990 Dec | Although corticosteroids (CS) cause nitrogen wasting in healthy humans, it is not known whether the salutary antiinflammatory and appetite-stimulating effects of CS in inflammatory diseases mitigate this effect. We measured nitrogen balance before, during, and after 3 d of high-dose methylprednisolone therapy in nine patients with flare-ups of rheumatoid arthritis. There was evidence of preexisting somatic protein and fat depletion in seven of nine subjects. Patients were allowed to eat freely on a metabolic ward. Nitrogen balances were -0.89 +/- 1.38 g/d (means +/- SEM) before CS therapy, -5.77 +/- 1.30 g/d during therapy (P less than 0.001), and -3.54 +/- 1.38 g/d after therapy (P less than 0.01) despite increased energy and nitrogen intake and clinical resolution of inflammation during and after the pulse therapy. We conclude that patients with rheumatoid arthritis are often cachectic and high-dose CS cause nitrogen wasting in these patients despite an antiinflammatory and appetite-stimulatory benefit. |