Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 3527035 | Oncogenes and autoimmunity. | 1986 May | Vertebrate cells harbor genes (proto-oncogenes) which carry the potential to become dominant transforming genes or oncogenes. Evolutionary conservation is the hallmark of these genes which implies that they have a major role in the growth and differentiation of the cells. In vitro activation of various cell types (immune cells, fibroblasts, etc.) leads to increased expression of various oncogenes in a certain temporal sequential order. Lymphoid cells from mice with autoimmune disorders have been shown to exhibit increased oncogene expression. Mononuclear cells from patients with angioimmunoblastic lymphadenopathy and certain autoimmune disorders (systemic lupus erythematosus and rheumatoid arthritis) express increased quantities of certain oncogenes. In this review, we discuss the role of oncogenes in the activation of immune cells and the pathogenesis of human autoimmunity. | |
| 2472849 | Quantitative detection of class-specific rheumatoid factors using mouse monoclonal antibod | 1989 Aug | An enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of rheumatoid factors (RF) was developed using mouse monoclonal antibodies against human IgG, IgA and IgM together with the biotin/streptavidin enhancement system. One hundred and eight patients with rheumatoid arthritis (RA) of whom 47 had a positive serum latex agglutination assay, 100 healthy controls and 95 diseased controls (25 systemic lupus erythematosus (SLE), 25 ankylosing spondylitis, 20 osteoarthritis and 25 bronchial asthma) were evaluated for the presence of IgG-, IgA- and IgM-RF by ELISA. Elevated levels of IgG-, IgA-, and IgM-RF could be demonstrated in respectively 94%, 91% and 98% of serum samples from RA patients with a positive latex test and in 72%, 69% and 8% of serum samples from RA patients with a negative latex test. In the latter patient group, increased levels of one or more RF isotypes could be detected in 82% of the patients. Except for the presence of IgG-RF in serum of 20% of the SLE patients, increased levels of RF isotypes were present in less than 5% of the diseased controls. Highly significant correlations were found between serum IgM-RF levels as detected by ELISA and those of the latex and the Rose-Waaler agglutination assays. Positive correlations were also found between the serum levels of the three RF isotypes investigated and between the levels of RF isotypes as measured in serum and synovial fluid of the same patient. Compared to agglutination assays, the ELISA for the quantitative detection of RF isotypes is a more reproducible and sensitive test which avoids some of the problems encountered in earlier ELISA methods. | |
| 3260427 | [Rational HLA-A, -B, -C typing in rheumatology]. | 1988 Apr 1 | In the own laboratory of our department from 1984-1987 367 patients with several rheumatic diseases were tissue-typed for HLA-A, B, C-antigens. Associations with diseases and clinical parameters were examined. We could only find significant associations (P corr. less than 0.05) of HLA-B 27 with reactive arthritis and iliosacral arthritis. We conclude, that the B 27-test is sufficient for economic HLA-typing in rheumatology. | |
| 2384010 | Serum interleukin 2-receptor levels in uveitis. | 1990 | This study reports on serum interleukin 2-receptor (IL-2R) levels in uveitis patients. IL-2R is the mediator protein of interleukin 2 (IL-2), a mitogenetic cytokine of primarily immune cell interaction. Stimulated T-cells readily express IL-2R on their cell surface and release a soluble form into serum. The measurement of IL-2R serum levels was performed using a monoclonal antibody based ELISA with age and sex matched healthy blood donors serving as the control group. The increase of IL-2R serum levels in patients affected by heterochromic cyclitis Fuchs (HCF) and a small group of children with chronic anterior uveitis affected by juvenile rheumatoid arthritis (JRA) was statistically highly significant, when compared to the control group (n = 84, alpha = 0.01). Serum levels of patients with intermediate uveitis (IU), patients with HLA-B27 positive acute anterior uveitis (AAU) and patients with HLA-B27 negative AAU did not differ significantly from the controls. | |
| 2282658 | Pregnancy and rheumatoid disease. | 1990 Apr | The onset of pregnancy appears to result in the amelioration of rheumatoid disease activity. This is most likely to be related to the change in hormonal, and thus immunological, status of the mother. Spontaneous abortion appears to be increased in mothers with rheumatoid disease. Whether this is due to disease status or to drug therapy is not entirely clear for all cases, but there is a suggestion that it could be the disease state. If at all possible, drugs should be avoided during pregnancy but simple anti-inflammatory drugs in low dosage probably do not produce any major problems. Immunosuppressant drugs should be avoided at all times except when the mother's health is at serious risk. The continued use of disease remitting agents throughout pregnancy is probably not necessary and there is still sufficient question as to whether these drugs could be potentially toxic to the infant. Primum non nocere. | |
| 2481878 | Pathogenetic mechanisms in the Mycoplasma arthritidis polyarthritis of rats. | 1989 | Mycoplasma arthritidis is the causative agent of severe polyarthritis in rats and mice, which resembles human rheumatoid arthritis (RA). Several mechanisms are involved in this disease. M. arthritidis releases substances acting on polymorphonuclear granulocytes (PMNs), i.e. oxygen radical formation stimulating substances (500-3,000 daltons), a chemotactic substance (400 daltons) and an aggregating substance (500 daltons). These products were separated from the cell-free culture supernatant by gel chromatography on Sephadex G-15 and G-10 columns. Isolated membranes of M. arthritidis possesses toxic properties for rats, mice, and chicken embryos. Hemolytic activities for sheep red blood cells and toxic effects on fetal rat skin fibroblasts were detected for this 170,000 dalton substance. Cross-reactivity between M. arthritidis and rat tissues was demonstrated in several investigations with polyclonal and monoclonal antibodies. Polyclonal antibodies against M. arthritidis showed a strong reaction in immunofluorescence tests with rat chondrocytes. In Western blot analysis six corresponding protein bands were observed in M. arthritidis membranes and rat chondrocytes, favoring the idea of several shared epitopes. Monoclonal antibodies were established reacting with M. arthritidis as well as with rat and human chondrocytes in the immunofluorescence test and in the enzyme immunoassay. Cross-reactivity could be observed also on the cellular level. T-cell lines of the OX 19 and W 3/25 type were established that could be stimulated by M. arthritidis antigens and by syngeneic chondrocytes. In the initial stage of the arthritis, toxic processes seem to be predominant that are continued by autoimmune reactions in the progressing disease. | |
| 3051093 | The other arthritides. Roentgenologic features of osteoarthritis, erosive osteoarthritis, | 1988 Nov | Osteoarthritis may be divided into primary generalized and secondary forms. Primary generalized osteoarthritis is characterized by narrowing of cartilage, marginal osteophytes, and absence of erosions. The most common sites of involvement are the distal interphalangeal joints of the fingers and the first carpometacarpal joint. Secondary osteoarthritis also results in narrowing of cartilage in the absence of erosions, but in regions of mechanical stress. Erosive osteoarthritis affects predominantly the proximal and distal interphalangeal joints, and evolves into bony fusion in 12 to 15 per cent of cases, about the same percentage of interphalangeal bony fusion that occurs in psoriatic arthritis. Ankylosing spondylitis predominates in the axial skeleton where it eventually leads to fusion of the vertebrae and sacroiliac joints. Psoriatic arthritis combines many features of rheumatoid arthritis, in which synovial inflammation predominates, and ankylosing spondylitis, in which ligamentous inflammation predominates. The hands and feet are involved to an equal extent, and in 20 per cent of patients the disorder also involves the sacroiliac joints and spine. Reiter's disease, like psoriatic arthritis, differs from ankylosing spondylitis in its inconstant involvement of the spine and greater involvement of peripheral joints. Reiter's disease differs from psoriatic arthritis in its predominant involvement of the lower limbs, particularly the feet, with relative sparing of the hands and wrists. Multicentric reticulohistiocytosis is a rare disorder in which polyarthritis usually precedes the onset of nodular cutaneous eruptions, a fact that emphasizes the importance of early roentgenologic recognition. The interphalangeal joints are the predominant sites of involvement in the hands, but eventually all of the synovium lined joints become affected, with arthritis mutilans the end result in one third of cases. The erosions are strikingly symmetrical and well circumscribed, and accompanying osteoporosis is disproportionately mild. Progressive systemic sclerosis is characterized by atrophy and dystrophic calcifications in the soft tissues, ultimately leading to joint deformities and resorption of the terminal tufts of the phalanges. Resorption of bone occurs at other sites as well, and marginal erosions may develop in the metacarpophalangeal and interphalangeal joints of the hands. | |
| 2143968 | Different strains of donor parental lymphoid cells induce different models of chronic graf | 1990 Sep | The induction of a chronic graft-versus-host (cGVH) disease in (Balb/c x A/J)F1 mice by the intravenous injection of either Balb/c or A/J parental lymphoid cells led to the development of two different models of disease. In this paper we compared the clinical aspects and the antigen specificities which recognized the autoantibodies developed by the animals of these two models of cGVH disease. Renal disease, alopecia, and purpura lesions were common in both models, although their frequency and intensity varied between groups. The models were differentiated by two main characteristics. When donor cells were of Balb/c origin, a joint disease similar to rheumatoid arthritis developed in 50% of the animals, and when donor cells were of A/J origin, 25% of the animals developed edema of the front feet, occasionally with loss of the nails, similar to that of scleroderma. Differences among the autoantibodies found in the sera of these two groups of mice were also observed. After the injection of Balb/c lymphoid cells, rheumatoid factors reactive with human and murine IgG were characteristically present (69 and 75%, respectively) and a statistically significant correlation was found between high titers of rheumatoid factor and arthritis (P less than 0.001). Antinuclear antibodies (ANAs) were present in all animals. Anti-dsDNA and anti-histones were positive in 50 and 25%, respectively. Anti-snRNP were detected at a low titer in 35% of the animals. When donor cells were of A/J origin, ANAs were also present in all mice. Anti-dsDNA, anti-histones, and anti-snRNPs antibodies were present in 90, 15, and 65%, respectively. The most outstanding characteristics among anti-snRNPs were the high titers of anti-U1 and anti-U3 detected in 50 and 30%, respectively. Rheumatoid factors reactive with human and murine IgG were positive in 15 and 42% of animals, respectively, but no significant correlation was found between these factors and disease. Our results indicate that the graft-versus-host disease induced in the same F1 strain of mice can be manifested in different forms of connective tissue disease, depending on whether the cells come from one or the other of the parental strains. Furthermore, in this paper the occurrence of rheumatoid factors in mice with cGVH is described for the first time. | |
| 3409645 | Sexual dysfunction among patients with arthritis. | 1988 Mar | The relationship of arthritis and sexual dysfunction was investigated among 169 patients with rheumatoid arthritis, osteoarthritis and spondyloarthropathy, 130 of whom were pair-matched to controls. Assessments of marital happiness and depressed mood were also made using the CES-D and the Azrin Marital Happiness Scale (AMHS). Sexual dysfunctions were found to be common among patients and controls, the majority in both groups reporting one or more dysfunctions. Impotence was more common among male patients than controls and was found to be associated with co-morbidity and the taking of methotrexate. Depressed mood was more common among patients and was associated with certain sexual difficulties, but not with impotence. Marital unhappiness, as indicated by AMHS scores, was not associated with arthritis but was associated with sexual dysfunction, sexual dissatisfaction and being female. | |
| 1829749 | IL-1ra ELISA: reduction and alkylation of synovial fluid eliminates interference by IgM rh | 1991 Jul 5 | IL-1 and a specific receptor antagonist of IL-1, IL-1ra, may play important roles in the pathophysiology of rheumatoid arthritis and in other types of inflammatory synovitis. Measurement of IL-1ra in synovial fluids and in other body fluids may lead to a greater understanding of its possible activity as a modulator of the immune and inflammatory systems in vivo. Therefore, a modified sandwich ELISA was developed to measure IL-1ra protein concentration in synovial fluids. The antibodies used in this ELISA were polyclonal and derived from rabbits hyperimmunized with human recombinant IL-1ra. IgM rheumatoid factors within synovial fluid resulted in false elevation of determined IL-1ra by the sandwich ELISA through binding of the primary and secondary antibodies. Reduction and alkylation of synovial fluid samples before application to the ELISA plate eliminated the interference caused by greater than or equal to 2000 micrograms/ml IgM rheumatoid factor (latex agglutination titer of 1/5.120). This ELISA was specific for IL-1ra; there was no detection of IL-1 alpha, IL-1 beta, or lysozyme. The sensitivity of this ELISA was less than 200 pg/ml, making it a useful assay for the accurate measurement of synovial fluid IL-1ra protein concentration. | |
| 3041971 | Immunopharmacological studies of new 3-benzoyl-4-mercaptobutyric acids. Immunomodulating e | 1988 Apr | A number of D-penicillamine (PA) derivatives (3-benzoyl-4-mercaptobutyric acids) having acetylthio groups on an alpha or beta position of a carboxylic acid, were synthesized and examined for their immunological effects compared with PA. New PA derivatives suppressed adjuvant-induced arthritis (AA) in SD rats and enhanced AA in Lewis rats like PA. Suppressive effects of 2-acetylthiomethyl-3-(4-methyl-benzoyl)propionic acid (compound II-3) on AA in SD rats was most potent among PA derivatives and PA. II-3 enhanced type II collagen-induced arthritis in rats more effectively than PA, and it slightly prolonged the survival time of NZBXNZW hybrid (BWF1) mice. Hemolytic plaque forming cells in the spleen cells of BDF1 and aged Balb/c mice were potentiated but those of BWF1 were suppressed by both compounds. In in vitro experiments, both compounds enhanced lymphocyte transformation. On the contrary, II-3 had no effect on the acute inflammatory response, delayed type hypersensitivity and IgE antibody response. The abnormal release of lysosomal enzymes from the peritoneal macrophages of aged MRL/l mice were suppressed by both compounds. These results suggest that II-3 is an immunomodulator like PA but more effective than PA. II-3 may be clinically effective for rheumatoid arthritis. | |
| 3283828 | [Sonography of the shoulder joint. Study technic, sonomorphology and diagnostic significan | 1988 Feb | In the evaluation of the impingement syndrome, conventional radiography can only show secondary bony changes. Sonography is able to demonstrate the anatomy of the musculotendinous structure of the rotator cuff. Lesions can be detected with the same accuracy and specificity as by contrast arthrography. As sonography is a non-invasive method, it should be used routinely to test rotator cuff integrity. Joint effusions and proliferative synovial changes are easily demonstrated by sonography. It is thus possible to perform follow-up studies in a pre-erosive stage of rheumatoid arthritis. | |
| 2188353 | [Imaging of seronegative spondyloarthritis]. | 1990 May 5 | The seronegative spondyloarthropathies (ankylosing spondylitis, psoriatic arthritis, Reiter's syndrome and arthritis associated with chronic inflammatory bowel diseases) are distinguished by inflammatory lesions of the sacroiliac joints, of the spine and/or a peripheral arthritis associated with the absence of rheumatoid factors. The author describes the radiological features of this group of diseases and their differential diagnosis. | |
| 3320211 | Fatal Pasteurella multocida septicaemia following a cat bite in a man without liver diseas | 1987 Nov | We report a fatal case of septicaemia caused by Pasteurella multocida that followed a cat bite. The patient, a man aged 54 years, was not suffering from disease of the liver but had a history of ischaemic heart disease, non-insulin-dependent diabetes mellitus and rheumatoid arthritis which had been treated with steroids for 13 years. | |
| 3492984 | Transcutaneous electrical nerve stimulation in experimental acute arthritis. | 1987 Feb | Transcutaneous electrical nerve stimulation (TENS) is applied for the relief of various types of pain, including rheumatoid arthritis and osteoarthritis. This study evaluated the influence of TENS on intraarticular (IA) temperature and pressure and synovial tissue of inflamed rabbit joints. Four hours after induction of acute bilateral hind joint arthritis by single intraarticular injection of urate crystals, the knees of 14 anesthetized rabbits were fixed at 90 degrees between femur and tibia. TENS was supplied from an electrical stimulator to the left knee joint while the other joint was unstimulated as a control. A microprobe needle for temperature measurement was inserted into the knee joint. Intraarticular pressure was recorded by polygraph connected to an IA needle. Readings were carried out at 10-min intervals three times before and four times after 5 min of TENS. A significant increase in IA temperature from mean 36.2C to 36.6C and significant reduction of IA pressure (mean decrease of 0.8 mmHg, from 2.5 mmHg to 1.7 mmHg) were found in the stimulated joints, which also showed a reduction of synovial fluid volume and total leukocyte count in comparison to the controls. While the nonstimulated synovial membrane showed massive leukocytic infiltration with neutrophilic exudate and fibrin clot in the intraarticular cavity, the stimulated synovial membrane demonstrated moderate homogeneous leukocytic infiltration through all layers, with absence of inflammatory exudate in the intraarticular cavity. These results suggested that the analgesic effect of TENS on arthritis may be partially attributed to decrease in IA synovial fluid pressure, volume, and leukocyte count. Therefore, TENS may be useful for reducing pain from inflammatory arthritis. | |
| 2347161 | Silastic joint arthroplasty of the great toe. A review of 228 implants using the double-st | 1990 Jun | Various operations have been advocated in the treatment of hallux valgus (bunions) and other painful conditions affecting the great toe, e.g., osteoarthritis, rheumatoid arthritis, and deformity. Resection arthroplasty using a double-stemmed silicone implant has proved a satisfactory method. A retrospective review of 228 implants followed over a period of eight years (average, four years) showed complete patient acceptance in 89%. | |
| 2270974 | Sparing effect of hemiplegia on scleroderma. | 1990 Dec | The paretic limb is spared in patients who develop rheumatic diseases after a hemiplegic stroke. This has been described previously in rheumatoid arthritis, gout, and osteoarthritis. A similar presentation in a case of scleroderma is described in this report. Scleroderma skin changes are absent in the completely paretic limb and were markedly reduced in the weak left leg. Inflammation may be modified either by neuropeptides or by an anatomical neurological lesion and this may explain the phenomenon. | |
| 2387524 | Mesenteric arteritis. | 1990 Aug | Four patients are presented with small bowel infarction secondary to a vascular arteritis. In three patients there was a history of rheumatoid arthritis. In each patient infarcted bowel was resected and a primary anastomosis performed. In one patient the anastomosis broke down and she subsequently died. One patient died from a disseminated rectal tumour three years later. The remaining patients remain well. If operated on early, intestinal infarction due to arteritis has a good prognosis. | |
| 3422859 | Perilesional linear atrophic streaks associated with intralesional corticosteroid injectio | 1987 Nov | Perilymphatic atrophy can be a complication of intralesional corticosteroid injections given for the treatment of conditions such as psoriasis, alopecia areata, and keloids, and intraarticular corticosteroid injections given in diseases such as rheumatoid arthritis. It may become manifest as perilesional linear, depigmented, atrophic streaks, which are usually most prominent in patients with dark-colored skin. | |
| 3751245 | [Experiences with external fixation of the cervical spine in the halo-yoke system]. | 1986 May | The Halo-Yoke-System allows an external fixation, reposition and immobilisation of the cervical spine. Indications for application of application of Halo-Yoke exist in conservative or operative treatment of acute traumatic lesions and protection of results after surgical treatment in cases of instability of different etiology (tumour, spondylitis, rheumatoid arthritis, congenital deformities). The experience basing on 28 own cases is reported. |