Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 3009530 | Quantitative estimation by a standardized enzyme-linked immunosorbent assay of human T-cel | 1986 Feb | Sera from patients with adult T-cell leukemia and asymptomatic carriers of human T-cell lymphotropic virus type I (HTLV-I) from widely separated areas of the world reacted strongly in a standardized quantitative enzyme-linked immunosorbent assay procedure with HTLV-I viral antigen prepared from a strain isolated in the United States. There was a sharp differentiation of the values seen in the patients as compared with a normal population. Of the 35 acquired immune deficiency syndrome patients with Kaposi's sarcoma, only 2 were positive for HTLV-I antibodies in this test, and the distribution of the negative assay values in the other acquired immune deficiency syndrome patient sera was similar to that seen in the normal sera. Sera which contained extremely high levels of antibodies to other unrelated viruses (rubella virus, cytomegalovirus, and herpes simplex virus) all showed negative anti-HTLV-I results, in a pattern similar to the normal sera. Sera from patients with several autoimmune disease (systemic lupus erythematosus, rheumatoid arthritis, thyroiditis) as well as those with infectious mononucleosis or myeloma all also showed the normal distribution of negative results, in spite of the presence of very high levels of the autoantibodies, etc., associated with their illnesses. | |
| 1718396 | Influence of fixation and immunohistological technique on accuracy, precision and inter-ob | 1991 Sep | Recently two highly sensitive and specific diagnostic criteria for Sjögren's syndrome based on percentages of IgA-, IgG-, and IgM-containing plasma cells measured in immunohistologically stained labial salivary gland tissue have been described. The reliability of such a criterion is dependent on the accuracy, precision and inter-observer reproducibility in plasma cell counting. The present study evaluates the effect of tissue fixation and immunohistological procedures on the aforementioned factors. Immunoglobulin (Ig)-containing plasma cells in sections of lamellated submandibular salivary gland tissue, alternately fixed in a 4% buffered formol solution or formol-sublimate solution and stained with an indirect immunoperoxidase and unlabelled peroxidase anti-peroxidase (PAP) method respectively, were enumerated by three independent observers. Relative numbers of Ig-containing plasma cells appeared to be less sensitive for systematic errors due to tissue fixation and immunohistological procedure than absolute numbers of Ig-containing plasma cells. The best inter-observer reproducibility of plasma cell counts was obtained in sections from formol sublimate-fixed specimens stained according to the PAP procedure. | |
| 3690428 | [Peripheral neuropathy in severe mixed cryoglobulinemia syndrome]. | 1987 Nov | A syndrome of polyneuropathy and cryoglobinemia is reported in a 76-year-old woman. Nerve biopsy showed severe demyelination and vascular involvement was demonstrated in a muscle biopsy. Peripheral neuropathy is discussed in the context of the Gougerot-Sjögren syndrome, associated with cryoglobins in the blood. Prompt treatment of this disease should be initiated and consists mainly of plasmapheresis. | |
| 3114453 | Expression of class I and class II major histocompatibility complex antigens on oral mucos | 1987 Mar | The expression of the Class I and Class II major histocompatibility antigens on oral mucosa was investigated using type-specific monoclonal antibodies. Class I antigen expression varied between various sites and in uncomplicated inflammatory responses appeared to be suppressed. HLA-DR antigen expression was only present on epithelial cells in the presence of extraneous immunocompetent cells. Oral mucosa did not express HLA-DQ antigens. The expression of Class I and Class II antigens varied between disease states exhibiting proliferative or destructive tissue responses. The pattern of Class I and Class II antigen expression is consistent with either MHC-restricted cellular interactions and subsequent destruction of epithelial cells or a protective role in non-destructive conditions. | |
| 3485182 | Anterior ischaemic optic neuropathy. A correlative clinical and visual evoked potential st | 1986 Feb | The clinical and visual evoked potential (VEP) findings were analysed in 18 patients with anterior ischaemic optic neuropathy. The VEP studies showed a variety of abnormalities which could be interpreted as being the result of subcomponent interaction consequent upon loss or attenuation of the normal macular-derived P100 component. Delay of normal VEP subcomponents was not seen. The VEP findings were non-specific but pointed to a severe disturbance of transmission in optic nerve fibres subserving central vision. No significant changes were observed with time in most cases indicating a static monophasic process with no significant recovery. | |
| 3109021 | Blood mononuclear cells in patients with primary Sjögren's syndrome: production of interl | 1986 | Disturbed immunoregulation is considered an essential feature of the pathogenesis in primary Sjögren's syndrome (primary SS). After in vitro stimulation we investigated the interleukin-1 production by blood monocytes, interleukin-2 production by blood T-lymphocytes, the number of IL-2 receptor-bearing blood T-lymphocytes and the blood lymphocyte DNA synthesis in 6 consecutive female patients with primary SS and in 6 female controls. In the patient group we found all the parameters examined within a wider range as compared to the controls. The three patients with reduced interleukin-2 production and low proliferative responses were exclusively characterized by elevated plasma-IgG concentration, positive antinuclear antibodies and rheumatoid factor, and by having circulating immune complexes. | |
| 2491631 | B cell hyperresponsiveness in Sjögren's syndrome. | 1989 | We examined the production of and responsiveness to B cell growth factor (BCGF) by using proliferation assays in patients with Sjögren's syndrome (SS). We also studied the ability of SS B cells to respond to B cell stimulatory factors (BSF) by measuring the amount of IgG produced in vitro. SS patients showed normal production of and responsiveness to BCGF. They were also demonstrated to show a normal response to Staphylococcus aureus Cowan I (SAC). In addition, cell surface antigens of B cells in the peripheral blood of SS patients were not altered compared with normal controls. However, SS B cells produced a significant amount of IgG in vitro in response to BSF stimulation with or without anti-IgM antibody. Furthermore, there was a positive correlation between the hyperresponsiveness of B cells to BSF in vitro and serum IgG level in SS. We, therefore, suggest that the enhanced response of B cells to BSF might contribute to polyclonal B cell activation in SS. | |
| 3742875 | Therapeutic effects of nandrolone decanoate, tibolone, lynestrenol and ethylestrenol on Sj | 1986 May | Growth of mononuclear cell infiltration in submandibular glands is significantly inhibited by Org OD14 (tibolone), lynestrenol and ethylestrenol given orally to New Zealand Black/White (NZB/W) mice from 26 weeks of age onwards. In addition, the extent of already established mononuclear cell infiltrations is significantly inhibited and reduced by nandrolone decanoate injected from 43 weeks of age onwards. Tibolone and nandrolone decanoate are the most potent of the four drugs. The therapeutic effect of these four steroids on the Sjögren's syndrome-like disorder in NZB/W mice is not related to their endocrine activities. | |
| 3495871 | Monoclonal anti-idiotypic antibodies against monoclonal rheumatoid factors derived from pa | 1986 | Monoclonal anti-idiotypic antibodies (mA-IDs) were produced against IgM-K, IgA-L and IgA-K monoclonal rheumatoid factors (mRFs), respectively, derived from patients with Sjögren's syndrome (SS). Each mA-ID inhibited the RF activity of the autologous M-protein, but did not inhibit the activity of the other mRFs. As for the specificity of mA-IDs, it was confirmed to bind only to the mRF used for immunization. A cross-reactive idiotype (CRI) was detected in 3 out of 106 polyclonal RFs (pRFs) using AW5/3 mA-ID by double immunodiffusion or ELISA inhibition assay on pRF activity. However, no CRI was detected on AMB1/5 or SF18/2 mA-ID. One of the mA-IDs, AW5/3, cross-reacted with nuclei of proliferating lymphocytes, such as PHA-stimulated lymphocytes, interleukin 2 dependent T cell lines, B-lymphoblastoid cell lines or cultured lymphoma cell lines, but barely reacted with nuclei of normal resting lymphocytes. | |
| 1903247 | Detection of anti-Ro(SSA) antibodies by gel double diffusion and a 'sandwich' ELISA in sys | 1991 Feb | A newly described Ro 'sandwich' ELISA was compared to the gel double diffusion technique to detect anti-Ro(SSA) antibodies in Sjögren's syndrome, systemic and subacute cutaneous lupus erythematosus patients. This study demonstrates that the ELISA assay increased the frequency of detection of anti-Ro(SSA) antibodies in these well defined connective tissue disease patients by approximately 5-10% compared to the gel double diffusion anti-Ro(SSA) antibody assay. The study also confirms that some patients make anti-Ro(SSA) antibodies directed solely at unique human Ro(SSA) antigen epitopes. We also detected the existence of a significant Sjögren's syndrome patient population failing to make significant anti-Ro(SSA) antibodies. We conclude from our study that the gel double-diffusion technique employing human spleen extract as a source of the Ro(SSA) antigen is, at present, the most cost-effective test to detect anti-Ro(SSA) antibodies. | |
| 2788543 | Autostimulatory growth factors produced by Sjögren's syndrome B cell lines. | 1989 Oct | Sjögren's syndrome (SS) is a systemic autoimmune disorder characterized by polyclonal hypergammaglobulinemia, autoantibody formation, and intense tissue infiltration by lymphoid and plasma cells. These patients have a predisposition to the development of monoclonal immunoglobulins and B cell lymphomas. To study the role of B cells in this disease, we established B cell lines from three patients with benign primary SS. These B cell lines grew spontaneously without any stimulation and expressed EBV nuclear antigen. The lines secreted an autostimulatory factor which had properties of a B cell growth factor. Peripheral blood B cells from SS patients, after culture for 3 days, secreted a similar factor which stimulated the proliferation of established SS B cell lines. These results suggest that circulating B cells in SS produce an autocrine growth factor that may contribute to lymphoproliferation and ultimately to the emergence of B cell lymphomas. | |
| 2575023 | Anti-CD3 and anti-CD2-induced T-cell activation in primary Sjögren's syndrome. | 1989 Sep | Because T-cell dysfunctions have been reported in patients with primary Sjögren's syndrome (SS), peripheral blood mononuclear cell (PBMC) proliferation obtained with anti-CD3 and anti-CD2 monoclonal antibodies was evaluated in these patients. Anti-CD3-induced mitogenesis, which varied widely among the patients, was lower in subjects with evidence of anti-SSA and anti-SSB antibodies than in controls. Moreover, the anti-CD2-induced response was depressed in about half the patients and the nonresponders were mainly those with anti-SSA and anti-SSB antibodies. Phorbol myristate acetate, a protein kinase C activator, used alone or added to anti-CD3, induced greater proliferation in patients than in control PBMC. In contrast, exogenous recombinant interleukin 2 (rIL-2) did not significantly enhance the anti-CD2-induced response of patients' PBMC, as it did in normal PBMC. Peripheral blood and parotid T cells from a patient with well-defined primary SS and parotid enlargement also responded poorly to anti-CD2 stimulation. Exogenous rIL-2 restored T-cell proliferation only in the salivary gland cultures of this patient. The present findings suggest that there is a T-cell activation defect in subjects with primary SS, particularly in those with circulating anti-SSA and anti-SSB antibodies. In addition, the difference in the response to IL-2 of peripheral blood and parotid-infiltrating T cells would seem to indicate that T-cell subsets are differently distributed in the blood and inflammation site. | |
| 2789573 | [Long-term study of patients with severe forms of Sjögren syndrome and/or myoepithelial s | 1989 Aug | It is well known that the incidence of malignant lymphomas (non-Hodgkin type) is increased in patients with Sjögren's syndrome. The occurrence of malignant diseases was retrospectively studied in 62 patients. In the course of the disease two patients developed a non-Hodgkin lymphoma, one patient developed a transitional-cell carcinoma located in the nasopharynx and three patients developed a squamous-cell carcinoma located in the salivary glands. The large quantity of epithelial carcinomas seems to be remarkable, especially compared with the number of non-Hodgkin lymphomas. It might be possible that early surgical treatment of persistent or progressive swelling of the salivary glands helps to prevent the occurrence of malignant lymphoma in patients with known myoepithelial sialadenitis. | |
| 3126283 | Immunohistologically definable light chain restriction in autoimmune disease. | 1988 Jan | Analysis of serum immunoglobulins from patients suffering from autoimmune disease has shown that pathogenically relevant autoantibodies directed at organ specific antigens are light chain restricted, i.e., they are entirely lambda or kappa type in a given patient. Furthermore, plasma cells involved at tissue level in the production of such antibodies, for example in Graves' disease, have also been shown to express a marked light chain bias as judged immunohistologically. On the basis of these findings, a study was conducted to determine the light chain status of tissue plasma cell infiltrates associated with Sjögren's disease, a known autoimmune disease. Of the six cases examined, all six showed a marked lambda light chain bias, with two patients exhibiting a monotypic plasma cell infiltrate of IgA, lambda isotype. The significance of the overall observations is discussed in the context of other examples of light chain restricted B-cell responses and the generally increased incidence of benign and malignant B-cell neoplasia in autoimmune disease. | |
| 3115203 | Sjögren's syndrome, vasculitis, and cryoglobulinaemia associated with a monoclonal IgM (k | 1987 Jun | A patient is described with primary Sjögren's syndrome with an infrequently reported complication--vasculitis and cryoglobulinaemia due to a monoclonal IgM (kappa) paraprotein with rheumatoid factor activity. The clinical and theoretical implications are discussed. | |
| 3522080 | Connective tissue disease in the elderly. | 1986 Apr | The prevalence of the inflammatory disorders of connective tissue in the older age group is quite likely underestimated at the present time; and, in most instances, age-related factors influencing clinical expression and autoreactivity have not been intensively studied. The array of connective tissue disease that usually targets the young adult emerges in the older age group as well; and, selectively in the elderly, GCA and PMR are found. The association of malignancy with PM/DM is increased with advanced age. In view of the therapeutic implications, early diagnosis is essential, but this can be especially difficult in the setting of problems related to the ageing process. | |
| 1997532 | Monocytoid B cell lymphoma. | 1991 Jan | The clinical, light microscopic, ultrastructural, immunocytochemical and cytogenetic features of a case of monocytoid B cell lymphoma were investigated. The tumour initially affected the cervical and supraclavicular nodes, but 33 months later affected the left parotid salivary gland. The patient had subclinical Sjögren's syndrome. The neoplastic cells showed characteristic morphological features and had peri- and interfollicular distribution in the node. Immunocytochemically the tumour cells were L26, 4KB5, MB2, CD19, CD20, CD22 and IgM/kappa positive. Prominent plasmablastic plasmacytoid differentiation was present in the recurrent tumour, suggesting an origin from post-follicular B cells. The lymphoma cells showed unusual cytogenetic abnormalities. | |
| 2101803 | Activation antigens in patients with Sjögren's syndrome. | 1990 | We report findings of a study of two receptors on mononuclear cells from patients with Sjögren's syndrome (SS). The two receptors, interleukin-2 receptor (IL-2), transferrin receptor (TfR), were identified using monoclonal antibodies anti-IL-2 and OKT9. We found that the IL-2 and TfR positive cells were significantly higher on mononuclear cells (MC) from peripheral blood (PB) of patients with SS than in healthy controls. The study revealed that the IL-2 and TfR positivity reflects systemic immune activation and correlates closely with the activity of SS. | |
| 2477001 | Autoepitopes reactive with anti-SS-B/La. | 1989 Aug | Sera from 120 patients with suspected autoimmune rheumatic disease and antinuclear antibodies of anti-SS-B/La specificity were examined by Western blotting for reactivity with the SS-B/La polypeptide of HeLa cells and recombinant SS-B/La derived from a 1.4 kilobase (kb) cDNA encoding approximately 90% of the SS-B/La molecule. All sera reacted with the HeLa cell and the recombinant SS-B/La. One hundred and fourteen (95%) reacted with a set of three Staph. aureus V8 protease-resistant peptides of Mr 30,000, 29,00 and 28,000 from a methionine-rich region of HeLa cell SS-B/La designated the X domain, and 98 (82%) reacted with another set of two protease-resistant peptides of Mr 24,000 and 23,000 from a phosphorylated region of HeLa cell La designated the Y domain. One reacted weakly with the Y domain only. All sera that reacted with X and Y reacted more strongly with X, suggesting that X was the major epitope. Antibodies affinity purified from the X domain reacted strongly with the X peptides but not with the Y peptides and conversely, antibodies affinity purified from the Y domain reacted with the Y peptides but not with the X peptides. Both antibodies reacted with a fusion protein comprising 102 amino acids at the carboxyl terminus of the SS-B/La molecule. This protein contained no methionine, demonstrating that methionines were not involved in the antibody-binding site. Over 80% of patients whose only criteria for selection was the presence of anti-SS-B/La had the clinical, histologic, serologic and phenotypic features of Sjögren's syndrome whilst the remaining 20% had at least two of the features.(ABSTRACT TRUNCATED AT 250 WORDS) | |
| 3051097 | Some extra-articular manifestations of arthritis and complications of therapy. A pictorial | 1988 Nov | More than 100 years ago Lasegue wrote, "Rheumatic fever licks at the joint but bites at the heart." This truth may be expanded to include nearly every joint ailment on one hand, and nearly every organ system on the other hand. In connective tissue disorders, bleeding diasthesias and metabolic and endocrine disorders, the joint manifestations are only one part of the total picture. This article shows a few examples of cardiopulmonary, gastrointestinal, and other manifestations. A few examples of iatrogenic complications are also described. The list of the examples described in this article is far from complete. |