Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 6712297 | IgE class immune complexes in Felty's syndrome: characterisation of antibody activities in | 1984 Apr | By means of a double polyethylene glycol (PEG) precipitation and PRIST technique IgE was detected in 3% PEG precipitates and in the immune complex enriched fractions purified by solid-phase Clq adsorption from sera of 11 of 20 patients with Felty's syndrome. No correlation was found between the occurrence of complexed IgE and total protein content of the immune complex enriched material. IgE rheumatoid factor and anti-IgE antibody activity were detected in some of the immune complex fractions. Serum levels of complement C3, C4, and factor B were low in IgE immune complex positive cases. Only 4 of 20 patients with articular rheumatoid arthritis had IgE-containing immune complexes. | |
| 6192594 | [Biological rhythms of various hormones and plasma protein parameters in the course of res | 1983 Mar 18 | Three groups of male patients with primary chronic polyarthritis, degenerative arthroses and labile hypertension took part in the study. They undertook spa treatment in three different health resorts. General aim of the study was to obtain information about biological rhythms of epinephrine, norepinephrine and cyclic AMP excretion, as well as about the serum concentration of triiodothyronine, thyroxine, immunoglobulin M, complement C 3 and alpha 2-macroglobulin in correlation with the spa treatment. Further, the occurrence of homogenization was investigated. Epinephrine excretion rhythms with a period length of 24 hours, which were not observed during the first and second week of the cure, occurred in the third week. With regard to norepinephrine excretion the phenomenon described as homogenization appeared. Furthermore, circaseptan rhythms, which could be evaluated as reactions to the spa treatment, were found. The occurrence of the circaseptan rhythms, however, cannot at once be equated directly with a positive result in response to the course of treatment. | |
| 2990781 | Serum antibody reacting with placental syncytiotrophoblast in sera of patients with autoim | 1985 Jun | This study reports the immunohistological detection of serum antibody reacting with the basal aspect of syncytiotrophoblast of human chorionic villi, where SSAV/GaLV (simian sarcoma associated virus/gibbon ape lymphoma virus) type C retrovirus p30 related antigen was observed by an indirect immunofluorescent method using monospecific antibodies against SSAV p28 and GaLV p29. The immunoglobulin class of this antibody activity called 'the anti-basal aspect of syncytiotrophoblast (anti-BAST)', was exclusively IgM and detected in the sera of both female and male patients with SLE and other autoimmune diseases, but rarely in the sera of normal controls. Immunofluorescent absorption and blocking test revealed that anti-BAST specifically reacted with human placenta and cross-reacted with subhuman primate type C RNA retrovirus SSV/SSAV (simian sarcoma virus/simian sarcoma associated virus), but did not cross-react with ATLV (adult T cell leukaemia virus) and BaEV (baboon endogenous retrovirus). These results suggest the presence of a new antigen-antibody system for another human type C retrovirus related antigens(s) and a participation of retrovirus in autoimmune diseases. | |
| 7023748 | Enzyme immunoassay of antibodies to Sjögren's syndrome B antigen. | 1981 Aug | I describe a solid-phase enzyme immunoassay of IgG-IgA-, and IgM-type antibodies to Sjögren's syndrome B antigen. Polystyrene tubes are coated with the purified antigen. The antibodies are allowed to bind with their antigens, and then detected with alkaline-phosphatase-conjugated anti-human IgG, IgA, or IgM sera. The amount of alkaline phosphatase fixed to the tubes is determined in pH 10.0 diethanolamine buffer at 37 degrees C with p-nitrophenyl phosphate as substrate. The absorbance of the p-nitrophenolate ion liberated in 1 h at 37 degrees C is measured at 406 nm. When rheumatoid factor is present, the values obtained for IgG antibodies are too low, and those for IgM antibodies are too high. | |
| 6603619 | Naturally occurring cytotoxic human antibodies recognize H-2-controlled murine lymphocyte | 1983 Jul | Human sera contain cytotoxic naturally occurring (CyNa) antibodies which discriminate between lymph node cells from mice differing only at the H-2 complex. Sera from three healthy subjects (normal human sera, NH sera) and one serum from a patient with multiple sclerosis reacted with cells expressing Db, Kd, Kk, and Kp molecules, respectively. However, the following observations suggested that the binding specificity of these CyNa antibodies is to antigens that are distinct from the classical H-2 antigens: (i) the NH sera did not contain cytotoxic anti-HLA antibodies, (ii) redistribution (capping) of H-2 antigens did not induce resistance to lysis for CyNa antibodies, and (iii) individual variation was demonstrated in the expression of the murine lymphocyte antigens detected by the human CyNa antibodies. The reason for this variation appeared to be different for individual NH serum. A maternal effect influenced the expression of the murine lymphocyte antigen detected by one NH serum (anti-H-2b). The differences detected by another NH serum (anti-H-2p) appeared to be inherited, as shown by progeny testing. We hypothesize that the human CyNa antibodies may be directed against antigens controlled or modified by murine viruses (milk borne or endogenous), whose expression is under the influence of the H-2 complex, and that their production might have been stimulated by the products of human genes homologous to murine viruses. | |
| 6262921 | [Studies of the basal secretion and the response to TRH (thyrotropin-relapsing factor) of | 1981 May 8 | The present data relate the studies of TSH and Prolactin secretion in response to TRH (500 microgram, IV) in Cushing's syndrome. Twenty-five patients were explored before treatment (4 adrenal carcinoma, 5 adrenal adenoma, 2 Cushing's diseases with patent pituitary tumor, 14 Cushing's diseases without patent pituitary tumor). Thirteen of the twenty-five patients were studied after treatment of their hypercorticism. Identical studies were realized in 5 patients receiving oral corticotherapy. The results, compared with those obtained in normal subjects are: the TSH basal values are normal or low. The TSH response is lower in untreated Cushing's disease (p2a : 0,01) and normal or low in other cases. The Prolactin basal values are significantly higher in untreated adrenal carcinoma (p2a : 0,05) and in Nelson's syndrome (p2a : 0,01), normal or high in other cases. The prolactin response before treatment is significantly higher in male Cushing's diseases (p2a : 0,05), high or normal in other cases, and unchanged in Nelson's syndrome. Those abnormalities of basal values and/or responses of TSH and Prolactin do not seem sufficiently discriminant of the etiology of Cushing's syndrome especially tumoral causes, in particular pituitary causes. They seem connected to hypercorticism because of the treatment of the last one corrects them. But other factors must be concerned in the Nelson's syndrome case. | |
| 6171156 | Long-term antiarrhythmic therapy with acetylprocainamide. | 1981 Dec | Nineteen patients whose arrhythmias were initially suppressed with acetylprocainamide underwent long-term treatment with this drug. Eleven patients were still taking the drug at the end of 12 months. Drug withdrawal with substitution of a placebo caused an increase in ventricular premature beats. Thus, suppression of ventricular premature beats persisted for 1 year. The eight withdrawals from the study were due to death during the year (n = 6) or recurrence of arrhythmias. The deaths occurred in patients who were in New York Heart Association functional class II (one patient), III (three patients) and IV (two patients). Ventricular performance, assessed from systolic time intervals, improved with drug therapy and declined during drug withdrawal. Symptomatic effects were common, with seven patients requiring a reduction in dosage or discontinuation of therapy. Three patients treated for 3 years continued to show drug suppression of ventricular premature beats compared with the level during placebo substitution. Small amounts of procainamide were present in all patients because of in vivo deacetylation of acetylprocainamide. Many patients with good initial responses to this drug had recurrent arrhythmias during long-term therapy. For this reason, the usefulness of acetylprocainamide as an antiarrhythmic drug appears to be limited. | |
| 6712296 | Autoantibodies in the sicca syndrome (primary Sjögren's syndrome). | 1984 Apr | IgA and IgM rheumatoid factors as well as antibodies to Ro and La were measured in 37 patients with the sicca syndrome and 9 patients with Sjögren's syndrome secondary to rheumatoid arthritis. Positive results were found in 84% (IgA rheumatoid factor), 76% (IgM rheumatoid factor), 62% (Ro), and 50% (La) of patients with the sicca syndrome. There was no significant difference in the frequency of positive results in patients with glandular versus extraglandular disease. Antibodies to La invariably occurred in patients with antibodies to Ro. In addition, patients who were anti-Ro positive also had significantly higher IgA (p less than 0.05) and IgM (p less than 0.01) rheumatoid factor activity. Since 81% of the sicca patients were concordant for 3 of the 4 antibodies tested, production of these autoantibodies appears to be related. | |
| 6158139 | Correlation between postsialographical changes of salivary isoamylase levels in serum and | 1980 Apr | Salivary isoamylase values in sera were evaluated before and after sialography in 14 patients with Sjögren's syndrome (SS) and were compared with those in non-SS patients. Non-SS patients consisted of cases of systemic lupus erythematosus, rheumatoid arthritis and ulcerative colitis. In non-SS patients, salivary isoamylase 3 to 6 hours after injection of Conray-400 increased to 2.3 +/- 0.5 fold higher than that prior to the injection and returned to the previous values after 48 hours. On the basis of increases in salivary isoamylase after injection of Conray-400, SS could be divided into three groups: the high response group, the group showing the same values as in non-SS patients and the low response group. The high response group was further classified into two subgroups. Salivary isoamylase before injection in the high response group was higher in patients who showed mild mononuclear cell infiltration histopathologically in their sublingual glands and was lower in patients whose sublingual glands showed diffuse infiltration of mononuclear cells. Mononuclear cells had infiltrated severely in the sublingual glands of patients in the low response group, although they showed salivary isoamylase values before injection within the same ranges as in non-SS patients. These data suggest that both salivary isoamylase before injection and maximum salivary isoamylase increased significantly in cases of mild sialadenitis, the former value decreased with increasing infiltration of mononuclear cells into the sublingual glands, and both values were lower in cases of severe sialadenitis. | |
| 737016 | Complement-fixing hidden rheumatoid factor in children with benign rheumatoid nodules. | 1978 Nov | Rheumatoid nodules have been described in many children without evidence of clinical disease. These have been referred to as benign rheumatoid nodules (BRN). Except for one report, no serological abnormalities have been demonstrated. Four children with BRN have been studied. By means of a hemolytic assay, high titers of hidden rheumatoid factor (RF) have been found in the blood, i.e., 19S IgM RF detected after acid separation of IgM-containing fraction from the serum. The median hidden RF titer of the children with BRN was 1:362 and in healthy and disease controls was 1:7. The difference was significant at P less than 0.001. The results indicate that patients with BRN have active complement-fixing 19S IgM RF in their serum. Thus the association of hidden RF with BRN raises the possibility that the deposition of immune complexes (19S IgM-7S IgG) leads to small vessel vasculitis and nodular formation. | |
| 5014610 | The development of macrophages from large mononuclear cells in the blood of patients with | 1972 Apr | The origin and function of the increased of "atypical lymphocytes" which appear in the blood of patients with many inflammatory diseases is not known. Leukocyte suspensions from eight patients with systemic lupus erythematosus (SLE), five patients with other rheumatic diseases, and five patients with infectious diseases were pulse-labeled with tritiated thymidine (Tdr-(3)H) and sampled after 5 and 72 hr in vitro. Radioautographs indicated that 35% of the total large, nonphagocytic mononuclear leukocytes incorporated Tdr-(3)H during the initial 5 hr of culture. Tdr-(3)H-labeled large phagocytic or glass-adherent cells were observed only infrequently. After 72 hr one-third of the original number of Tdr-(3)H-labeled cells from patients with SLE developed the morphology of macrophages and the capacity to phagocytose latex particles. Similar findings were observed in patients with other rheumatic diseases and bacterial infections. In contrast, the thymidine-labeled cells from patients with infectious hepatitis and infectious mononucleosis were poorly viable in culture and rarely became macrophages. Tdr-(3)H-labeled small lymphocytes were uncommon. The present experiments suggest that in patients with certain inflammatory diseases large, proliferating "lymphocytelike" cells are very immature monocyte precursors which appear in response to tissue injury. These DNA-synthesizing cells together with mature monocytes may serve as the circulating source of macrophages. | |
| 6092094 | Induction of human interleukin 1 mRNA measured by collagenase- and prostaglandin E2-stimul | 1984 Oct | Human blood peripheral monocyte/macrophages release in culture a mononuclear cell factor (MCF) which stimulates the production of collagenase and prostaglandin E2 by human rheumatoid synovial cells and dermal fibroblasts. These two products play a role in connective tissue destruction. MCF has an apparent molecular weight of approximately 15 000 and is biologically and biochemically indistinguishable from interleukin 1. MCF therefore belongs to the well-documented nonimmune biological activities attributed to interleukin 1. Studies on the mechanisms of production and action of such monokine(s) have been difficult in view of the minute quantities produced by freshly isolated cells or from human monocytic lines. Starting from lectin-stimulated human blood mononuclear cells, we have isolated poly(A)+ RNA and studied its translation following microinjection into Xenopus laevis oocytes. The mRNA translation products stimulated collagenase and prostaglandin E2 production in human rheumatoid synovial cells and dermal fibroblasts. The size of MCF-mRNA was estimated to be 10 S. The mRNA of a member of the interleukin 1 family can now be studied in a system based on a specific and direct relevant biological assay and eventually compared with those of other monokines. | |
| 1123372 | The relative safety of polymethylmethacrylate. A controlled clinical study of randomly sel | 1975 Jan | Forty patients from a group of seventy elected to participate in a prospective randomized clinical study designed to evaluate the systemic effects of polymethylmethacrylate implanted with a Charnley total hip replacement, utilizing patients treated with a Ring total hip prosthesis ad controls. Intraoperative alterations in cardiovascular function were related to use of methylmethacrylate, but postoperative changes in pulmonary function, erythrocyte sedimentation rate, serum lactic acid dehydrogenase, serum glutamicoxalacetic transaminase, and alkaline phosphatase were not. Charnley total hip replacements were associated with more ectopic-bone formation twelve months postoperatively. The two "latent" infections recognized during the thirty-four months of the study were in hips without acrylic fixation. | |
| 1123722 | Comparative activities, tolerances and safety of nonsteroidal anti-inflammatory agents in | 1975 Jan | Relative anti-inflammatory potencies of aspirin, phenylbutazone, indomethacin, three fenamates and several other nonsteroidal anti-inflammatory agents were obtained in several laboratory models of acute and chronic inflammation. Relative toxicities and ulcerogenicities were determined in rats of the same source, strain and sex. The acute ulcerogenic assay measures the minimal irritation potential of these agents and leads to a sensitive index of the safety of such compounds when compared with their therapeutic potencies. By these criteria, meclofenamic acid is a highly potent, acceptably safe and exceptionally well tolerated anti-inflammatory-antipyretic agent in rats when compared with other such drugs. | |
| 803366 | Nephrotoxic effect of parenteral and intraarticular gold. Ultrastructure and electron micr | 1975 Jan | The presence of gold in the kidney of a 58-year-old woman was confirmed by use of ultrastructural and microprobe examinations. A series of animal experiments were performed in which small single doses of gold were administered systemically and intraarticularly in rabbits. Both the colloidal suspension and the soluable salt gold sodium thiomalate (Myochrysine) were used. Selective lesions occurred in the proximal convoluted tubules and the mitochondria appeared to be the target organelle. They contained gold after gold sodium thiomalate but not after colloidal gold. | |
| 4364334 | Hormonal control of lysosomal enzyme release from human neutrophils. Effects of autonomic | 1974 Jun 1 | The purpose of this investigation was to examine the effects of autonomic neurohormones, cyclic nucleotides, and related agents on the immunologic discharge of lysosomal enzymes from, and phagocytosis by, purified human neutrophils. In order to discern the possible intracellular mechanisms by which certain neurohormones influence neutrophil function, the concentrations of cyclic AMP and cyclic GMP in neutrophils were assessed during cell contact with phagocytizable particles and autonomic agents. The model system employed for study was the interaction of purified human neutrophils with rheumatoid arthritic (RA) serum-treated zymosan particles at 37 degrees C in a neutral, balanced salt solution containing glucose. Neutrophils ingested the particles and discharged beta-glucuronidase but not lactate dehydrogenase activity during 30 min of incubation. Treatment of zymosan particles with RA serum was more effective than treatment with normal serum with regard to the extent of both particle uptake and lysosomal enzyme release. During contact of neutrophils with RA serum-treated zymosan particles epinephrine, isoproterenol, and cyclic AMP inhibited both particle ingestion and beta-glucuronidase discharge. These actions of epinephrine were associated with a concomitant elevation of cyclic AMP levels. In contrast to the actions of catecholamines and cyclic AMP, acetylcholine and cyclic GMP accelerated lysosomal enzyme release without affecting particle uptake. The actions of acetylcholine were associated with a concomitant elevation of cyclic GMP levels. Increases in neutrophil levels of cyclic GMP but not of cyclic AMP were associated also with the discharge of beta-glucuronidase provoked by particles in the absence of added cholinergic agents. The data suggest that the immunologic release of lysosomal enzymes from human neutrophils can be regulated by autonomic neurohormones, perhaps via the selective formation of appropriate nucleotides. | |
| 629242 | Mixed connective tissue disease in childhood. Relationship Sjögren's syndrome. | 1978 Mar | Mixed connective tissue disease (MCTD) seems to be a distinct entity that has some manifestations of systemic lupus erythematosus, scleroderma, polymyositis, and Sjögren's syndrome and is serologically characterized by the presence of an antibody to ribonucleoprotein. We report the cases of three children with MCTD with high titers of antibody to ribonucleoprotein. Two fulfilled criteria of lupus erythematosus, two had polymyosis; all three had suggestive features of scleroderma, fulfilled criteria for the diagnosis of juvenile rheumatoid arthritis, and had Sjögren's syndrome. Additional superimposed features of another connective tissue disease should arouse suspicion of MCTD. All three of our patients responded adequately to corticosteroid treatment that makes recognition of this entity by the pediatrician all the more important. | |
| 3898478 | Dry eyes: autoimmunity and relationship to other systemic disease. | 1985 | Sjögren's syndrome is one of the more common connective tissue diseases, perhaps second in frequency only to rheumatoid arthritis. Autoimmunity in Sjögren's is suggested by its close clinical association with other connective tissue diseases and autoimmune disorders, the dense lymphocytic infiltration of the exocrine glands and the presence of circulating autoantibodies in the majority of cases. Antibodies to the cellular ribonucleoproteins, Ro(SSA) and La(SSB) appear to have clinical significance in this condition, since not only do they identify patients at risk of developing Sjögren's syndrome but also they may have a pathogenetic role in this disease, at least in some of the systemic manifestations. | |
| 6224885 | Monoclonal IgM rheumatoid factors derived from arthritic MRL/Mp-lpr/lpr mice. | 1983 Sep 1 | MRL/lpr/lpr (MRL/l) mice develop a lupus-like syndrome and a disease histologically and serologically similar to human rheumatoid arthritis. Their sera contain polyclonal IgM rheumatoid factors (RF) reactive with all murine IgG subclasses (frequently strongest with IgG2a) and several heterologous IgG. To examine the repertoire and epitopic specificities of these RF, we fused splenocytes from 3.5-mo-old seropositive MRL/l mice with appropriate myeloma partners and derived 1,723 hybridomas of which 23 secreted IgMRF. These monoclonal IgMRF bound to murine IgG only, not to other murine isotypes. Eight murine IgG subclass-specific clonotypes were identified. Most clones reacted with either multiple IgG subclasses or with IgG2a alone. A few clones reacted solely with IgG2b but none reacted exclusively with IgG1 or IgG3. Monoclonal IgMRF with exclusively anti-IgG2a activity exhibited allotypic specificity, reacting, with few exceptions, with a, c, and e, but not b, d, or j IgG2a allotypes. Four clonotypes could be distinguished by cross-reactivity with IgG from species other than mice. Monoclonals possessing activity against several murine subclasses cross-reacted extensively with heterologous IgG, including all human IgG subclasses without allotypic restrictions. Monoclonal IgMRF specific for murine IgG2a or 2b did not cross-react with heterologous IgG. Based on the absence of cross-reactions by IgG2a-specific monoclonal autoantibodies, certain peptides of the IgG CH2 and CH3 domains appear to generate the antigenic determinants of the anti-IgG2a RF in MRL/l mice. All of the monoclonal RF bound to Fc and, with one exception, not to Fab fragments of murine IgG. Binding of the monoclonal RF to substrate IgG was not inhibited by Clq, thus excluding the Clq-binding site at the CH2 domain as one of the responsible epitopes in the induction of MRL/l RF. mIgMRF could be categorized as strongly, weakly, or noninhibitable by protein A, which interacts with IgG molecules at or near the CH2-CH3 junction. Inhibition appears to be caused by conformational changes and/or steric shielding of certain IgG areas distant from this junction and not by identical binding sites between protein A and RF. Certain of the mIgMRF that were weakly or not at all inhibitable by protein A were found to cross-react equally well with human Fc (CH2-CH3 domains) and pFc' (CH3 domain) fragments, indicating that the binding site for these monoclonals is at the CH3 domain. Monoclonal RF were devoid of anti-double-strand DNA, anticollagen, or antipeptidoglycan pentapeptide cross-reactivity, but one of the monoclonals cross-reacted with histones, four with single-strand DNA, and one with both histones and single-strand DNA. | |
| 4563708 | Immunofluorescence studies on immunoglobulins in the lymphoid cells of human peripheral bl | 1972 Aug | Immunofluorescence was applied for the detection of intracellular immunoglobulins in human peripheral blood cells. The technique permitted the screening of large numbers of cells and the discrimination of two fluorochromes within each cell. In this way distribution patterns were obtained for the major immunoglobulin classes and for both light chain types. In contrast to the analogous bone marrow patterns there was no correlation with the distribution pattern of the immunoglobulins in the serum in the samples without paraproteinaemia. These results are compatible with the view that the bone marrow—but not the blood—is to be considered as a major source of the immunoglobulins in the serum. This hypothesis also implies that the initial phase of antibody production takes place in the peripheral lymphoid organs with a homing tendency of the immunoglobulin-producing cells in the bone marrow. The findings in paraproteinaemia support this view. Information about the quantitative contribution of the peripheral lymphoid organs is insufficient to draw definite conclusions. It became furthermore apparent that there can exist an intracellular imbalance with regard to the heavy versus the light chain components of the immunoglobulin molecules. The result often is an H/L ratio in excess of unity. |