Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 3266356 | Serum IgG4 anti-Fab antibodies in rheumatoid arthritis are constitutively expressed. | 1988 | IgG4 comprises a significant proportion of the total anti-Fab antibody (aFABA) response in many but not all patients with rheumatoid arthritis (RA). Analyses of the dynamics of IgG aFABA subclass expression in 11 RA patients for periods of up to 11 months demonstrated that IgG4 aFABA was restricted to 6 of the 11 RA patients' sera initially studied and comprised approximately 25% (or more) of the total IgG aFABA response. Quantities of IgG4 aFABA in subsequent, serially obtained serum samples from these patients remained stable throughout the study period, whereas the remaining RA patients whose initial sera possessed small quantities of serum IgG4 aFABA failed to generate any augmented IgG4 aFABA response during the study. Elevated expression of IgG4 aFABA did not appear to be a consequence of a generalized polyclonal gammopathy or a generalized increase in autoantibody expression, though patients with higher total IgG4 serum levels expressed significantly greater quantities of IgG4 aFABA. These results indicate that the differential expression of IgG4 aFABA among RA patients reflects constitutive production within a subset of RA patients in whom IgG4 appears to comprise a significant proportion of the total IgG aFABA response. | |
| 3451917 | Rapidly progressive osteoarthritis of the hip. | 1987 Jun | Osteoarthritis of the hip is rapidly progressive in 5% of cases, particularly in patients of advanced age. At times there is total destruction of the femoral head. Nineteen such cases are described and the literature is reviewed. The clinical picture is dominated by rapidly progressive pain and limping; the radiographic picture by the absence of osteophytosis. The pathology of rapid progression is subdivided into primary and secondary. Several hypotheses of the pathogenesis are discussed. These are related to mechanical overloading factors, local cortisone therapy, anoxia, synovitis. Differential diagnosis is with subacute septic arthritis, monarticular rheumatoid arthritis, and idiopathic osteonecrosis of the femoral head. Prosthetic replacement is the only treatment indicated and the long-term results do not differ from those obtained in prosthetic replacement for the more common type of osteoarthritis. | |
| 3960280 | Autoantibodies to neuroblastoma cell surface antigens in neuropsychiatric lupus. | 1986 Feb | A human neuroblastoma cell line, LA-N-1 was used as a target cell in a I131 radiolabeled staphylococcal protein-A (I131-SpA) binding assay, to characterize the pattern of antineuronal activity of human sera in fifty-four cases of systemic lupus erythematosus (SLE) including twenty-six patients with neuropsychiatric manifestations of SLE (LE-CNS), out of which ten were pediatric patients aged eleven to eighteen years, thirty-six normal donors and sixteen rheumatoid arthritis patients. The IgG binding activity of normal control sera with LA-N-1 neuroblastoma cells was determined to be 998 +/- 490 cpm I131-SpA, per 5 micrograms LA-N-1 protein (mean +/- SD), 2936 +/- 2607 cpm I131-SpA per 5 micrograms LA-N-1 protein for rheumatoid arthritis patients, 5109 +/- 3304 cpm I131-SpA per 5 micrograms LA-N-1 protein for SLE patients. The binding activity of for LE-CNS patients sera was: 10 565 +/- 2993 and 15 346 +/- 2993 cpm I131-SpA per 5 micrograms LA-N-1 protein, for the pediatric and adult group of patients respectively. Absorption assays disclosed that the antineuronal IgG autoantibody detected in the LE-CNS group of patients is cross reacting with human adult brain, while the anti-neuronal activity of rheumatoid arthritis and SLE patients could be removed by sequential absorption with homogenates of human lung, liver, and kidney, fetal calf serum, human muscle and human lymphocytes. We conclude that detection of autoantibodies binding to LA-N-1 human neuroblastoma cells may be helpful in the diagnostic workup of pediatric and adult LE-CNS patients. | |
| 2018610 | [Oral lichen planus and Sjogren's syndrome. 2 cases of association]. | 1991 Jan | We described two female patients, aged 55 and 49 years respectively, who fulfilled the diagnostic clinical and histological criteria for lichen planus and Sjögren's syndrome. The association of either lichen planus or Sjögren's syndrome to autoimmune disorders seems not to be fortuitous and common pathogenic mechanisms have been proposed. In the literature, however, the association between lichen planus and Sjögren's syndrome has been infrequently reported. | |
| 2342016 | GUEPAR total knee prosthesis. Results and outcome of seven years' use in a district genera | 1990 Feb | Between 1980 and 1987, 44 total knee arthroplasties of the GUEPAR type were performed in 38 patients with severe degenerative joint disease. The patients have been followed up for 7 years and 70% of cases had a good or fair result. The 13 poor results included five cases of deep sepsis, three of whom subsequently underwent successful arthrodesis at this hospital. Other causes of a poor outcome were loosening and patellofemoral dysfunction. Radiological review of 27 knees showed that 80% had lucent lines at either cement/prosthesis or bone/cement interface, although these radiological findings were not associated with clinical evidence of loosening. The major long-term complication was shown to be retropatellar pain, which was recognized in 16% of all cases. However, this would seem to be the only significant long-term complication, and we believe that with the use of a patellar button the GUEPAR prosthesis should continue to be used in selected cases of severe degenerative joint disease. | |
| 2825483 | Leukotriene B4 metabolism in human leukocytes: fact or artefact? | 1987 Aug | The object of this study was to investigate the importance of omega oxidation in regulating leukotriene B4 (LTB4) levels in man. In human polymorphonuclear leukocytes metabolism of LTB4 was rapid but was critically dependent on PMN number: greater than 1.5 X 10(6) PMN/ml were required. Metabolism of LTB4 was blocked in the presence of plasma. In whole blood and in PMN-rich rheumatoid synovial fluids no significant metabolism of LTB4 was detected within 30 min at 37 degrees C. We conclude that LTB4 metabolism at inflamed sites will be regulated both by cellular content and the degree of plasma exudation. In most pathological conditions rapid exchange with the micro-vasculature will be more important than metabolism in limiting LTB4 levels. | |
| 3804522 | Clinical experience with total arthroplasty and hemiarthroplasty of the shoulder using the | 1986 | A consecutive series of 28 shoulders in 26 patients, treated since 1982 by either total arthroplasty (11 shoulders) or hemiarthroplasty (17 shoulders), are reviewed. A glenoid osteotomy was also performed in five of the shoulders with a hemiarthroplasty. Overall 16 shoulders were completely satisfactory with little pain and satisfactory movement. Nine had a total arthroplasty. The average range of shoulder elevation after total arthroplasty (121 degrees +/- 29 degrees) was significantly better than after hemiarthroplasty (91 degrees +/- 39 degrees). Pain was still present in seven shoulders with a hemiarthroplasty but in only one with a total joint replacement. | |
| 2680166 | Measurement of lysozyme in human body fluids: comparison of various enzyme immunoassay tec | 1989 Oct | Three variants of the immunoenzymometric assay of human lysozyme with HRP-labeled antibodies were compared. The highest sensitivity (with a detection limit of 0.2 micrograms lysozyme/L) was achieved by a one-step assay lasting 2 h. Between-batch precision for the techniques was 6-11%. Lysozyme reference values were determined in serum, cerebrospinal fluid and urine. In serum they are age-dependent and in urine sex-dependent when related to creatinine excretion. Serum lysozyme is increased in only 57% of the patients with active rheumatoid arthritis and is also unreliable for indicating remission. In Crohn's disease the serum lysozyme reflects activity better, but it does not exceed the diagnostic value of alpha-1-acidic glycoprotein (orosomucoid). The lysozyme quantification in cerebrospinal fluid is useful in distinguishing between viral or bacterial meningitis. | |
| 2150359 | [Mechanism of treating rheumatoid arthritis with polyglycosides of Tripterygium wilfordii | 1990 Jun | The in vitro effects of polyglycosides of Tripterygium wilfordii Hook (T II) on immunoglobulin (Ig) production by peripheral blood mononuclear cells (PB-MC) from seventeen healthy persons and eight rheumatoid arthritis (RA) patients were studied. PBMCs, monocytes and lymphocytes were treated separately with different doses of T II for different periods of time. Cells in different combinations were cultured for two weeks under pokeweed mitogen (PWM) stimulation and the IgG and IgM content of culture supernatants were determined using the double antibody solid-phase immunoradiometric assay. There was no difference in the inhibitory effect of T II on in vitro IgM and IgG production by PB-MCs either between the two types of Ig or between healthy individuals and RA patients. T II acts both on monocytes and lymphocytes, but more strongly on the latter. This observation appears to differ from the reported actions of gold and penicillamine. The results obtained in this study correlate well with those of clinical trials, suggesting that the therapeutic effect of T II is associated with its immunosuppressive activity. | |
| 3039039 | Detection of Epstein-Barr virus strain variants in lymphoblastoid cell lines 'spontaneousl | 1987 Aug | 'Spontaneous' lymphoblastoid cell lines (LCL) were established from patients with either rheumatoid arthritis (RA) or infectious mononucleosis (IM) or from healthy donors. Differences in Epstein-Barr virus (EBV) strains were determined by measuring the mol. wt. and expression of viral antigens in each of the LCLs. In addition to the previously reported EBV nuclear antigens, the LCLs also contained EBV-induced antigens with mol. wt. of 48K and 58K which were present in all but two of the lines. One of the differences observed between each of the groups of cell lines was their ability to produce viral antigens. Early and late antigens were identified by immunoblotting in most of the RA lines, two of the normal lines but none of the cell lines from patients with IM. Many of the IM cell lines were also found to express multiple EBNA1 antigens. The results demonstrate that a variety of wild-type EBV strains exist. However, the similarities observed in a number of the lines suggest that the diversity of strains may be limited. | |
| 3192565 | Internal fixation for occipito-cervical fusion. | 1988 Nov | We describe a method of internal fixation for occipito-cervical fusion utilising a standard "small fragment" T-plate bent and fixed to the skull with three screws. The lower end of the plate is screwed and wired firmly to the spine of the axis. Of 14 patients so treated, 12 fused, one died and one failed to unite to the skull. Of eight with cord signs, seven remitted or improved and one died. | |
| 2948973 | Piroxicam-induced photosensitive dermatitis. | 1986 Dec | Eleven patients had piroxicam-induced photosensitivity when first seen. Six were photopatch-tested to piroxicam (2%, 5%, 20% concentrations); all tests were positive. This same group were patch test-positive to thimerosal. Three of the eleven patients became persistent light reactors. Two additional patients developed persistent hand dermatitis. | |
| 3733795 | Transoral decompression and posterior fusion for rheumatoid atlanto-axial subluxation. | 1986 May | Cervical myelopathy is an uncommon but potentially fatal complication of rheumatoid atlanto-axial subluxation. Computerised myelotomography with three-dimensional reconstruction shows that rheumatoid pannus, together with the odontoid peg, contributes significantly to anterior cervico-medullary compression. These findings were the basis for treatment by transoral anterior decompression and posterior occipitocervical fusion, which removes both bony and soft-tissue causes of compression and allows early mobilisation without major external fixation. We report encouraging results from this combined approach in 14 patients who had progressive neurological deterioration. | |
| 3516751 | Update on ibuprofen: review article. | 1986 | Non-steroidal anti-inflammatory drugs (NSAIDs) have become the principal mode of therapy for rheumatic diseases and their use has continued to increase despite concern expressed recently regarding potential hazards (Figure 1). Prior to 1969, a limited number of NSAID drugs were available. Aspirin and indomethacin became the mainstay of treatment but tolerability, particularly gastric irritation, at doses necessary to control rheumatic symptoms limited the usefulness of these valuable agents. The pyrazolone, phenylbutazone, showed slightly better gastro-intestinal (GIT) tolerability but has since been associated with an increased risk of blood dyscrasiae and is now only available for restricted use in most countries. Ibuprofen was the first of a new breed of NSAIDs originally introduced into the United Kingdom in 1969. Chemically quite distinct from its forerunners it was the first of the propionic acid derivatives to be used in rheumatic practice. The propionics have since become the largest, single and most important group of NSAIDs accounting for 50% of NSAID prescriptions in the United Kingdom. It is estimated that over 100 million patients worldwide have received ibuprofen which is now available in over 100 countries throughout the world including all the major markets. Ibuprofen was developed directly as a result of the problems associated with the use of corticosteroids in the treatment of rheumatoid arthritis and also because of the gastro-intestinal irritation and general intolerability of the established NSAIDs, at that time. Ibuprofen was readily accepted because, unlike the previous drugs, its therapeutic efficacy was easily seen to outweigh the severity of its side-effects. Ibuprofen was the first new drug with the potency of aspirin but without its major disadvantages. | |
| 3552729 | Effect of insulin on aminoisobutyric acid uptake by human non-rheumatoid and rheumatoid sy | 1987 Apr 20 | The biological sensitivity of cultured non-rheumatoid human synovial cells (NRSCs) and rheumatoid synovial cells (RSCs) was examined in terms of the ability of insulin to stimulate the uptake of alpha-aminoisobutyrate (AIB). NRSCs, like numerous fibroblastic lines, were sensitive to physiological concentrations of the hormone: half-maximal stimulation was obtained with (4 X 10(-10) M) insulin, while maximum transport was found with a 60-90 min association time. On the contrary, although the basal transport was similar in RSCs, insulin was totally unable to accelerate AIB transport in these cells. Inflammatory processes lead to an insulin resistance which most likely involves a post-receptor step at the cellular level. | |
| 1932512 | Soluble HLA-DR molecules in patients with HLA class II versus class I associated disorders | 1991 | HLA genes have been identified as key genetic factors contributing to many chronic diseases characterized by autoimmune features. The role of HLA encoded molecules in the pathogenesis of these diseases is unresolved. We have now analysed soluble HLA-DR molecules circulating in the serum of patients with different autoimmune diseases and have defined parameters controlling serum levels. Patients with HLA-DR associated diseases were characterized by elevated serum concentrations of HLA-DR molecules and were clearly distinct from patients with HLA-B27 associated disorders. We did not find evidence for a correlation between disease activity, laboratory abnormalities and elevated serum concentrations of soluble HLA-DR molecules. Studies in normal individuals indicated that soluble HLA-DR molecules are at least partially regulated by the HLA haplotype. Highest serum concentrations were found in individuals carrying the HLA-DR3 or HLA-DR4 haplotype raising the possibility that the phenomenon of HLA-disease association reflects differences in the genetic control of soluble HLA-DR molecules. Interferon-gamma treatment caused an increase in serum concentrations of soluble HLA-DR molecules, whereas a decrease of circulating HLA-DR molecules was associated with an immunosuppressive with cyclosporine A. These data suggest that the patient's immunoresponsiveness represents a second important mechanism controlling circulating HLA-DR molecules. | |
| 3280790 | Severe reversible interstitial pneumonitis induced by low dose methotrexate: report of a c | 1988 Jan | A patient treated with 7.5 mg methotrexate/week (MTX) for rheumatoid arthritis (total dose 300 mg) developed high fever, dry cough and progressive dyspnea and hypoxemia due to a severe interstitial pneumonitis. MTX was discontinued and an infectious etiology was ruled out by cultures, serology and lung biopsy. Corticosteroids administered intravenously in high dose led to a dramatic improvement and a complete amelioration of all symptoms and signs. Pulmonary toxicity is a rare adverse effect of low dose MTX therapy and a review of the literature reveals 6 additional cases. Since MTX induced pneumonitis is a potentially fatal, yet completely reversible, disease, attention should be paid to even mild early respiratory symptoms in patients treated with low dose MTX and patient monitoring should include pulmonary function tests. | |
| 2423279 | Anti-H1 histone antibodies in systemic lupus erythematosus: epitope localization after imm | 1986 Mar | Using micro enzyme-linked immunosorbent assay (micro-ELISA) anti-H1 antibodies are most frequently seen in systemic lupus erythematosus (SLE) (61.4% of patients). Positive anti-H1 ELISA reactions are rare in rheumatoid arthritis (RA) (5.8% of cases), melanomas (16.7%), leukaemias (13.6%) and other cancers (5.6%). In SLE, the immunoglobulins (Ig) which constitute anti-H1 antibodies are, by order of importance, IgM, IgG and IgA. By means of immunoblotting using H1 solutions digested by alpha-1-chymotrypsin fixed on collagen membranes, we have shown that all the SLE sera containing anti-H1 antibodies recognize the sequential epitopes that are found on the carboxy terminal tail and, for 28% of anti-H1 sera, also the epitopes present on the aminoterminal half. The technique used did not make it possible to determine with certainty whether anti-H1 autoantibodies are also directed against the conformational epitopes of the globular part of the molecule. | |
| 1677574 | Extravascular fibrin formation and dissolution in synovial tissue of patients with osteoar | 1991 Aug | Fibrin deposition is a prominent finding in the synovium of patients with rheumatoid arthritis (RA). Macrophages are found in increased numbers in RA synovium, and these cells are known to produce a variety of procoagulant and anticoagulant molecules. Using immunohistologic techniques, the content and distribution of several important components of the coagulation system in the synovium of patients with RA, osteoarthritis (OA), or traumatic joint abnormalities requiring surgery were investigated. Samples from 3 patients from each category were examined in detail. RA synovium (compared with that of patients with OA or joint trauma) had increased numbers of macrophages and increased expression/content of fibrinogen, tissue factor, factor XIII, tissue transglutaminase, cross-linked fibrin (fibrin D dimer), urokinase-type plasminogen activator, and alpha 2-plasmin inhibitor. Macrophage content in RA synovium was increased in both the lining cell areas and the interstitial cell areas. Fibrinogen was distributed throughout the tissue in all samples and was greater in RA synovium. In trauma and OA synovia, tissue factor was seen only in association with vessels (endothelial cells), but in RA synovium, it was markedly increased throughout the tissues. While fibrin D dimer was seen in small amounts in synovial lining cell areas of trauma and OA synovia, it was present in increased amounts in the lining cell and interstitial cell areas of RA synovium. Factor XIII and tissue transglutaminase were present in scant amounts in trauma and OA synovia, but there were increased amounts of both (especially tissue transglutaminase) in RA synovium in the vessel, lining cell, and interstitial cell areas. Urokinase and alpha 2-plasmin inhibitor were also markedly increased in RA synovium. These results suggest that in inflamed synovium, there is ongoing extravascular tissue fibrin formation and dissolution that correlates with the degree of inflammation and macrophage content. Extravascular coagulation/fibrinolysis in RA represents a potential target for therapeutic intervention in this disease. | |
| 3324306 | Immunoregulation in arthritis. A review on synovial immune reactions in RA and in some exp | 1987 | Local synovial immune reactions have during recent years been characterized both in human arthritides, particularly in rheumatoid arthritis (RA), and in animal models for arthritis. Common characteristics of human RA on one hand and experimental adjuvant arthritis and collagen arthritis on the other hand, are induced expression of class II transplantation antigens on synovial cells close to the cartilage and presence of activated T lymphocytes in close proximity to these class II expressing cells. The present review aims to describe some implications of these and subsequent findings both concerning the analysis of the pathogenesis of RA and concerning some therapeutic implications derived from parallel studies on relevant features of the human RA and the respective animal models for arthritis. |