Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 2534706 | Preventive aspects of mobility and functional disability. | 1989 | Among bone and joint diseases, frequent causes of disability include (a) fractures from falls and osteoporosis, (b) osteoarthritis of hips, knees, and lumbar spine, (c) painful feet, (d) back pain, and (e) intractable rheumatoid arthritis. Risk factors for falling may arise from factors intrinsic to the patient and from hazards in the environment. The prevention and management of osteoporosis are controversial. Most experts endorse the use of estrogens in postmenopausal women and the need for continuing physical activity, particularly walking, in both elderly men and women. To preserve independent living, conservative management of osteoarthritis of hip, knee, and low back consists of properly balancing rest, non-weight-bearing exercises, and the use of walking aids. In rheumatoid arthritis, the clinician should emphasize (a) exercises to maintain range of motion and (b) muscle strength, both to prevent flexion contractures and to maintain the ability to carry on activities of daily living. | |
| 1699625 | Estimation of serum V kappa IIIb light chains in rheumatoid arthritis and correlation with | 1990 Oct | The presence of V kappa IIIb light chains in the sera of rheumatoid arthritis (RA) patients has been evaluated by an enzyme-linked immunosorbent assay (ELISA). V kappa IIIb light chains have been confirmed to be largely restricted to IgM, and were rarely detected in the IgG fraction of sera. The concentration of total serum V kappa IIIb did not significantly vary with age, nor did it correlate with IgM-rheumatoid factor (RF) titre. Although total serum V kappa IIIb was not significantly increased in RA patients compared with matched controls, IgM-RFs frequently contained V kappa IIIb. Using flow cytometry, CD5-positive B-cells were not increased in these RA patients compared with healthy laboratory control personnel. Furthermore, there was no direct correlation between total serum IgM V kappa IIIb content and CD5-positive B-cell numbers in peripheral blood. | |
| 2796414 | An approach to developing a valid Spanish language translation of a health-status question | 1989 Oct | This article discusses methodological issues confronting health professionals using questionnaires to study health care variables among populations with limited literacy in English, and suggests techniques for minimizing problems that plague questionnaire-based research among these populations. A recent effort to validate a questionnaire for Rheumatoid Arthritis patients in South Texas is used to illustrate pitfalls and potential solutions. | |
| 2029202 | Psychological profiles of patients with upper gastrointestinal symptomatology induced by n | 1991 Apr | Eighty three patients with classical or definite rheumatoid arthritis taking non-steroidal anti-inflammatory drugs (NSAIDs) were studied in an attempt to determine whether a patient's personality and psychological profile might contribute to the development of NSAID induced gastrointestinal symptomatology. It was found that the personality profile of the group of 45 asymptomatic patients was similar to that of a previously reported control group. In contrast, the 37 patients with gastrointestinal symptoms attributed to NSAIDs had a significantly higher mean score for free floating anxiety, depression, and somatisation than controls, suggesting that a patient's personality may influence the development of NSAID induced gastric symptoms. | |
| 2821934 | Enhanced capacity for release of leucotriene B4 by neutrophils in rheumatoid arthritis. | 1987 Jul | The calcium dependent metabolism of endogenous arachidonic acid (AA) was investigated in 17 patients with rheumatoid arthritis during treatment with dextropropoxyphene alone and in 25 healthy volunteers. Incorporation of [1-14C]AA into intracellular phospholipids of purified neutrophils was achieved by incubation until steady state before activation with ionophore A23187. Analysis of extracellular metabolites was performed by extraction, thin layer chromatography, autoradiography, and laser densitometry. The patients showed a twofold increase in the total capacity for oxidation of AA. Release of leucotriene B4 (LTB4) and its omega oxidation products, 20-OH LTB4 and 20-COOH LTB4, was 29%, range 11-48%, in patients compared with 8%, range 4-12%, in healthy volunteers. Total amounts of radioactivity released and the specific activity of LTB4, as assessed by high pressure liquid chromatography, were equal in experimental and control groups. The demonstrated increased capacity for metabolism of AA to the major proinflammatory metabolite, LTB4, via the 5-lipoxygenase pathway may contribute to perpetuation of inflammation and to tissue destruction in rheumatoid arthritis. | |
| 1932507 | Activation of murine autoreactive b cells by interleukin 1-like factors released from syno | 1991 | The effect of interleukin 1 (IL-1)-like factor(s), produced by cells isolated from the synovial fluids of rheumatoid arthritis (RA) patients, on an in vitro murine model of spontaneous autoimmunity, i.e., the development of plaque-forming cells (PFC) to bromelain-treated mouse red blood cells (Br-MRBC) in mouse peritoneal cell (PC) cultures, has been investigated. It has been found that IL-1-containing culture supernatants from cells isolated from joint fluids of RA patients, as well as recombinant IL-1, determine a marked increase in anti-Br-MRBC PFC development. Moreover, factor(s) of 10-20 KD molecular weight, with IL-1-like biological activity, capable of increasing the anti-Br-MRBC PFC development in mouse PC cultures, have been demonstrated in joint fluids from RA patients. The finding that synovial inflammatory cells produce factors that activate autoreactive B cells further supports the role of autoimmunity in the pathogenesis of rheumatoid arthritis, as self-perpetuing disorder. | |
| 2512639 | Blood monocyte elastolytic activity enhanced by immune complex stimulation in patients wit | 1989 | Blood monocyte elastolytic activity in patients with rheumatoid arthritis and in normal controls was studied by a new in vitro technique. The enzyme activity of live cells was measured by soluble [3H]elastin hydrolysis first under basic conditions and then after immune complex stimulation. The cells from patients had a higher elastolytic potential than cells from controls (p less than 0.02) and responded to smaller amounts of immune complex (p less than 0.01), even in patients treated with D-penicillamine or aurothiomalate. Treatment of normal monocytes in vitro with aurothiomalate did not influence the elastolytic response. These findings indicate that monocytes from patients with rheumatoid arthritis have an enhanced elastolytic activity compared with cells from normal controls and may invoke greater tissue damage on immune complex stimulation. | |
| 2767736 | IgA rheumatoid factor and IgG dietary protein antibodies are associated in rheumatoid arth | 1989 Jul | This study sought to determine whether patients with rheumatoid arthritis (RA) were immunologically sensitised to dietary protein (DP). Using an enzyme linked immunosorbent assay (ELISA), antibodies to milk and wheat proteins were measured in 93 unselected out-patients with classical or definite RA. Of these 93, 53 had raised levels of IgG antibodies to one or both dietary proteins (DP). In the DP antibody positive group, 48 patients (90%) also had raised levels of IgA rheumatoid factor (measured by ELISA) while only 7 (17%) of the 40 DP antibody negative patients had detectable IgA RF; P less than 0.02. There was no association between IgM rheumatoid factor and dietary protein antibodies. These results demonstrate that in RA, raised levels of IgA RF are associated with an increased IgG response to antigens which enter the body through the gastrointestinal tract. A breakdown in gastrointestinal tolerance to dietary antigens may play a role in the immunopathogenesis of RA in these patients who might therefore benefit from dietary manipulation. | |
| 2815947 | [Orthopedic basic documentation: requirements of diagnostic statistics and results in Göt | 1989 Jul | For the first time the revised "Bundespflegesatzverordnung" (Federal Statutory Instrument on Hospital Care Rates) of 1985 has ordered all the hospitals in the F.R.G. to prepare annual statistics of diagnoses giving as part of the anonymous hospital statistics an overview about the medical variety of inpatients and enabling scientific evaluation of cases. The most important documentation items are patient's age, duration of stay, main diagnosis encoded according to ICD-9, and operation characteristic (whether or not any of the operations was carried out because of the main diagnosis). The computerized Goettingen documentation comprises all 15 items of the Minimum Basic Data Set of the European Communities and a few other additional details. The result is a comprehensive and in many directions useful medical documentation for which examples are given. | |
| 3549979 | Role of bone grafting in correction of protrusio acetabuli by total hip arthroplasty. | 1986 | A study of 27 hip arthroplasties derived from a pool of more than 200 total hip arthroplasties performed between 1976 and 1981 was conducted to assess the need for and benefits of bone grafting in the surgical management of protrusio acetabuli. The objective of surgery and the reason for augmentation by bone graft was to lateralize the acetabular component, normalize the center of rotation of the hip, and strengthen the deficient medial wall. Based on this study, the authors recommend: when protrusion is less than 5 mm in either direction and the medial wall is reasonably strong, bone graft is not indicated; in protrusion greater than 5 mm with a thin but intact medial wall, autogenous bone graft is indicated but artificial fixation devices need not be used; and a grossly deficient medial wall requires reconstruction with bone graft and additional fixation devices to achieve normalization of the center of rotation of the hip joint. | |
| 3510213 | Massive allografting for severe failed total hip replacement. | 1986 Jan | We are presenting the cases of five patients that illustrate the uses of large, frozen bone and osteoarticular allografts in dealing with severe structural deficiency about the hip joint associated with prior failed total hip replacement. The status of the grafts was assessed at a minimum follow-up of twenty-four months (average, thirty months). In four patients extensive grafts in the proximal part of the femur in conjunction with total hip-replacement components were used, and in the fifth patient a matched whole-joint transplant of the proximal part of the femur and the acetabulum was employed. The short-term clinical advantage of these massive grafts is clear, but the long-term results are uncertain. | |
| 2257449 | Specific antibody response to the mycobacterial 65 kDa stress protein in ankylosing spondy | 1990 Dec | Immune responses to conserved, immunogenic homologues of the mycobacterial 65 kDa stress protein (SP65) have been implicated in inflammatory arthritis. Serum anti-SP65 was measured in AS, RA and healthy controls using an indirect enzyme immunoassay with recombinant SP65. IgA anti-SP65 was elevated in 19 of 59 AS patients, but the elevation in median level was not statistically significant. Anti-SP65 of all isotypes was increased in RA, but achieved significance (P less than 0.01) for IgA only. Adjusting specific antibody results for elevations in total serum Ig levels reduced AS and RA anti-SP65 to near normal levels, suggesting that a major component of the increased anti-SP65 may be secondary to polyclonal activation. | |
| 3100338 | Pathways to chronic inflammation in rheumatoid synovitis. | 1987 Jan | Postcapillary venules resembling the high endothelial venules (HEVs) of lymphoid tissues have often been observed at sites of chronic inflammation. We have therefore postulated that such venules may be an important site of lymphocyte migration into rheumatoid synovial membrane and that inflammatory cell products may act on endothelial cells (ECs) to increase lymphocyte emigration. Electron microscopic examination of rheumatoid synovial membranes showed that a strong correlation existed between the proportion of lymphocytes in perivascular tissue and the height/base ratio of the ECs in those areas. In addition, binding experiments showed that peripheral blood mononuclear cells preferentially bound to ECs in sections of rheumatoid synovial membrane that had the morphological appearance of HEVs. In vitro binding experiments, in which lymphocyte adhesion to human umbilical vein EC monolayers was measured, showed that adhesion was enhanced by preincubation of the ECs with interferon-gamma or interleukin 1 (IL 1). The central role of IL 1 in increasing lymphocyte migration into the rheumatoid synovial membrane was also supported by the findings that IL 1 is chemotactic for lymphocytes, ECs can secrete IL 1, and IL 1 activity is readily detectable in synovial fluids of rheumatoid arthritis patients. | |
| 1683235 | Association of rheumatoid arthritis with a dominant DR1/Dw4/Dw14 sequence motif, but not w | 1991 Nov | HLA-DR, HLA-DQ, and T cell receptor beta (TCR beta) chain gene polymorphisms were investigated in 43 patients with rheumatoid arthritis (RA), in 10 patients with Felty's syndrome (FS), and in 5 RA multicase families. RA was found to be strongly associated with a DRB1 gene sequence motif present in DR1, DR4-Dw4, and DR4-Dw14 alleles. Ninety-three percent of RA patients were positive for at least 1 of these alleles, providing strong support for the "shared epitope hypothesis." The frequency distribution of this sequence motif suggests a dominant mode of inheritance. All 10 FS patients were DR4-Dw4 positive. Different DR-DQ associations among DR4 positive RA and FS patients indicate heterogeneity in the genetic susceptibility to these 2 disease entities. Furthermore, analyses of TCR V beta 8, V beta 11, and C beta gene polymorphisms did not support the notion of an influence of TCR beta germline allotypes on RA susceptibility. | |
| 3515918 | Flurbiprofen versus naproxen in the treatment of rheumatoid arthritis. | 1986 Mar 24 | The efficacy and safety of flurbiprofen (Ansaid, Upjohn), 100 mg twice daily, were compared with those of naproxen, 250 mg twice daily, in a six-week, double-blind, randomized study involving 133 patients with rheumatoid arthritis. Patients completing the six-week treatment phase were then treated with flurbiprofen, 100 mg twice daily, during a six-week open-label phase. In the double-blind phase, both treatment groups showed improvement from baseline and, in general, the arthritic condition of all patients was significantly less severe while receiving treatment. In the open-label phase, the patients in whom therapy was switched from naproxen to flurbiprofen reported greater improvement compared with baseline than they did at the end of the double-blind phase. Statistically significant differences between medication groups were few. At weeks four and six, grip strength for the naproxen group increased from baseline by a marginal amount compared with the flurbiprofen group. Global evaluations of disease improvement by patients and physicians and proximal interphalangeal joint size showed trends in favor of flurbiprofen. In the double-blind phase, 29.4 percent of flurbiprofen-treated patients (n = 20) and 23.1 percent of naproxen-treated patients (n = 15) experienced side effects, most of which were gastrointestinal in origin. In the open-label phase, 81.0 percent of the patients (n = 87) satisfactorily completed the six weeks of flurbiprofen treatment. Based on this study, 100 mg of flurbiprofen administered twice daily was as effective as 250 mg of naproxen twice daily in the treatment of rheumatoid arthritis. | |
| 3316580 | Total lymphoid irradiation in alloimmunity and autoimmunity. | 1987 Dec | Total lymphoid irradiation has been used as an immunosuppressive regimen in autoimmune disease and organ transplantation. The rationale for its use originated from studies of patients with Hodgkin disease, in whom this radiotherapy regimen was noted to induce profound and long-lasting immune suppression and yet was well tolerated, with few long-term side effects. Total lymphoid irradiation is a unique immunosuppressive regimen that produces a selective (and long-lasting) reduction in the number and function of helper T cells and certain subsets of B cells. Conventional immunosuppressive drugs show little selectivity, and their effects are short-lived. The most important aspect of total lymphoid irradiation is the potential for achieving transplantation tolerance and permanent remissions in autoimmune disease in laboratory animals. Attempts are being made to achieve similar goals in humans given total lymphoid irradiation, so that immunosuppressive drugs can be ultimately withdrawn from transplant recipients and patients with lupus nephritis. | |
| 1865122 | Methods for the detection of anti-endothelial antibodies by enzyme-linked immunosorbent as | 1991 Jul 26 | In order to obtain a routine simple screening test for the detection of anti-endothelial antibodies (AEA) we developed a highly reproducible and sensitive enzyme-linked immunosorbent assay (ELISA) on fixed endothelial hybridoma cells. Detection of AEA with this type of monolayer appeared to be superior to ELISAs with monolayers of human umbilical vein endothelial cells, unfixed endothelial hybridoma cells or assays with membranes of endothelial cells. Glutaraldehyde treated endothelial hybridoma cells are most appropriate for use in ELISA procedures to detect AEA because the endothelial hybridoma cells are easy to culture, form a constant antigenic source and when plated and fixed to microtitre plates they can be stored without losing their ability to bind AEA. | |
| 3596867 | A comparison of a sustained release preparation of tiaprofenic acid with the conventional | 1987 | In a randomized double-blind, multicentre crossover study, the short-term efficacy and tolerance of tiaprofenic acid (TA) conventional tablet 300 mg twice daily was compared with a sustained release preparation of tiaprofenic acid 600 mg once daily and with a placebo in 146 patients with rheumatoid arthritis. After a minimum washout period of three days, patients were randomly allocated to receive each treatment for a period of two weeks each in a crossover fashion. Pain levels, duration of morning stiffness, onset of inactivity stiffness, articular index, and grip strength, were measured at the end of the washout period and at the end of each treatment period. Escape analgesic consumption, patient compliance, patient preference and overall assessment were measured at the end of each treatment period. Side-effects were noted and standard laboratory tests performed. Sustained release TA was shown to be as effective as the conventional tablet TA in the treatment of rheumatoid arthritis, producing significant improvements in efficacy parameters measured. Both preparations were as well tolerated as the placebo. | |
| 2975107 | Suggested mode of action of D-penicillamine as an immunosuppressive agent in rheumatoid ar | 1988 | The mechanism by which D-Penicillamine is effective in the treatment of rheumatoid arthritis has been investigated. The results indicate that D-Penicillamine in synergism with copper or ceruloplasmin in vitro inhibits the proliferation of T-lymphocytes and the activity of helper T-cells in supporting the generation of antibody-forming cells. This effect is mediated by the production of H2O2. The significance of these findings for in vivo processes is discussed. | |
| 3363282 | Effect of aggregated IgG on mononuclear and polymorphonuclear cell cooperation. | 1988 | The leukocyte adherence inhibition (LAI) test was used to determine the response of different leukocyte subpopulations in rheumatoid arthritis (RA) patients and normal donors to heat-aggregated gammaglobulins (HAGGs). The adherence of polymorphonuclear cells of (PMNs) could be inhibited either directly by high concentrations of HAGGs or indirectly by soluble factors secreted by RA mononuclear cells incubated with low amounts of HAGGs. The stimulatory site of the IgG was located on the Fc fragment. Since the LAI reaction was also observed with material recovered from rheumatoid synovial fluid, tissue, and nodules, these results could have clinical relevance in RA. |