Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 3266681 | Serum level of parathyroid hormone in certain rheumatic diseases. | 1988 Oct | The mechanism of joint destruction in rheumatic diseases is a complex and not fully known phenomenon in which many factors probably take part. The hormones which regulate the bone metabolism may be engaged in this process. In this study the serum level of the parathyroid hormone was correlated with the degree of joint destruction observed in rheumatoid arthritis, ankylosing spondylitis and osteoarthritis. Besides the degree of joint changes (radiologic aspects) the extension of the pathological process and duration of disease were also considered and the serum and urine level of calcium and phosphorus was analyzed. Similar patterns of the parameter investigated were observed in the rheumatic diseases studied and in healthy persons. | |
| 1658909 | [Environmental chemicals: their clinical significance in rheumatology]. | 1991 Oct 22 | Over the past years, various illnesses including features of connective tissue disorders (e.g. systemic sclerosis, systemic lupus erythematosus, mixed connective tissue disease), and rheumatoid arthritis, seemingly related to exposure to environmental materials have been reported. This paper presents some typical case reports and review major environmental associations seen in rheumatology, along with their epidemiology, clinical and laboratory features, possible pathogenesis, prognosis, and potential therapy. | |
| 1813024 | Network theory of glycosylation--etiologic and pathogenic implications of changes in IgG g | 1991 Oct | It is now well established that glycoproteins are populations of individual glycoforms. While it has been inferred from in vitro experiments that the differential glycosylation of glycoproteins diversifies their function, evidence is lacking for such a role in vivo. Alterations in IgG glycosylation in both normal and disease states in vivo, however, provide strong evidence that glycosylation is not static and may be a highly regulated event. The large amount of data correlating disease activity and severity in autoimmune diseases which have a strong B cell component with changes in the incidence of IgG glycoforms, now suggest that glycoform population shifts may not be just a marker of disease activity, but may also contribute directly to disease persistence and pathogenesis. | |
| 3180575 | The Sheehan total knee arthroplasty. A retrospective review. | 1988 Nov | Sixty Sheehan total knee arthroplasties (TKA) in 44 patients were reviewed 3.5 years following surgery. Patient mobility was improved, but only 29 knees were completely free of pain. In 18 knees there was a complaint of pain localized to the patellofemoral joint, and in five knees the pain was severe enough to require further surgery. Complications included three knees with presumed deep infection and three knees with tibial stud fractures. Aseptic loosening did not occur, although sinkage of the tibial component was observed. Revision arthroplasty was not successful mainly because of poor residual bone stock. Salvage surgery included three successful arthrodeses. The high incidence of patellofemoral problems and violation of bone stock make the current surface replacements attractive alternatives for TKA. | |
| 3532405 | [Experimental substantiation of the treatment of patients with rheumatoid arthritis with p | 1986 | Fifty RA patients receiving prednisolone and prednisolone combined with retabolil were examined. Prednisolone made a negative effect on central hemodynamics, the effect being less with the use of retabolil. A dystrophic action of prednisolone on the myocardium and the weakening of this effect in a combined use of prednisolone and retabolil were shown on a RA model in rabbits. | |
| 1929580 | Prevalence of anticardiolipin antibodies in juvenile chronic arthritis. | 1991 Sep | The prevalence of anticardiolipin antibodies was evaluated in 70 children with juvenile chronic arthritis (JCA), in 25 adult patients with rheumatoid arthritis, in 42 healthy children and in 40 adult controls. Thirty seven (53%) patients with JCA were positive for IgG or IgM anticardiolipin antibodies, or both, and 30 (43%) for IgG anticardiolipin antibodies. In contrast, only seven (28%) adult patients with rheumatoid arthritis presented anticardiolipin antibodies, which were of IgG class in four (16%) cases. IgG anticardiolipin antibodies were negative in all control subjects while IgM anticardiolipin antibodies were detected in two (5%) children and in four (10%) adult controls. No correlations were found in patients with JCA between the presence or titres of anticardiolipin antibodies and various clinical or laboratory variables. No patient with anticardiolipin antibodies showed any feature of the anticardiolipin syndrome. | |
| 3501160 | HLA DR4 and B27 antigens in familial and sporadic rheumatoid arthritis. | 1987 | We studied HLA antigens in 105 unrelated American Caucasian patients with rheumatoid arthritis (RA), 70 of them with familial disease. HLA-DR4 was observed in 71% of familial RA, 63% of non-familial RA, and 27% of normal controls, confirming the already well-established association between HLA-DR4 and both familial and non-familial RA. HLA-B27 was present in 14.3% of patients, versus 8% of normal controls (p = 0.04), and was not more common in familial (10 of 70, or 14.3%) versus non-familial (5 of 35, or 14.3%) disease. These results are compared with those observed in Scandinavian patients. | |
| 2443573 | Detection of C1q-bearing immune complexes by a monoclonal anti-C1q ELISA system. | 1987 Sep 24 | A monoclonal antibody directed against the collagenous portion of human C1q was used to detect C1q-bearing immune complexes in patients with rheumatic disorders. Sera of patients with rheumatoid arthritis, systemic lupus erythematosus (SLE), osteoarthritis, as well as normal human sera (NHS) used as controls were tested in an ELISA system. C1q-bearing immune complexes were bound to a solid-phase monoclonal anti-C1q antibody, and detected with F(ab')2 antibodies to human IgG. Heat-aggregated human IgG was adjusted to the same concentration as the WHO standard for immune complexes and used for the standard curve in NHS. The mean value in NHS was 19.5 micrograms/ml equivalents of aggregated IgG. Using 2 SD over the mean as the upper limit for normal values, samples greater than 43 micrograms/ml were considered positive. Patients with osteoarthritis were negative; high levels of C1q-bearing immune complexes were detected in patients with rheumatoid arthritis (up to 800 micrograms/ml equivalents of aggregated IgG). With our assay C1q-bearing immune complexes were detected with high frequency (81%) in the sera of patients with rheumatoid arthritis, while a C1q solid-phase binding assay (C1q SPBA) revealed positive results only in 67% of rheumatoid arthritis sera. Compared to NHS, CH50 titers and C1q values of sera from patients with rheumatoid arthritis were frequently high. In contrast, the sera of SLE patients with low CH50 titers and low C1q levels had IgG immune complexes which could be detected only in the C1q-SPBA. C1q-bearing immune complexes were not detectable in the sera of patients with SLE. Since C1q triggers activation of the classical C pathway, this assay with monoclonal anti-C1q antibody appears to be useful for detecting immune complexes in rheumatoid arthritis patients with normal or elevated CH50 and C1q values, especially in the early stage of the disease. | |
| 1815421 | Steady-state pharmacokinetics of hydroxychloroquine in rheumatoid arthritis patients. | 1991 Dec | Steady-state pharmacokinetics of hydroxychloroquine (HC) sulfate (Plaquenil) were studied in five volunteers with rheumatoid arthritis who had taken 6 mg/kg/d of the drug for at least six months. Blood samples were drawn at 0, 1, 2, 4, 6, 8, 12, and 24 hours following an oral dose. Both whole blood and plasma were assayed by an HPLC method for HC and its metabolites desethylhydroxychloroquine, desethylchloroquine, and didesethylchloroquine. A 24-hour urine collection was obtained and assayed for the same compounds. The pharmacokinetics of HC and its metabolites conformed to the model predicted by single-dose studies. During the 24-hour period the absorption phase and both early and late distribution phases were seen. Variation in mean maximum/minimum concentration was 40 percent. Renal clearance accounted for only 16 percent of unchanged HC (22 percent of total drug and metabolites) and did not correlate with creatinine clearance. | |
| 1974181 | Activation antigens expressed on T-cells of the peripheral blood in Sjögren's syndrome an | 1990 May | We analyzed activated T-cells in the peripheral blood (PB) of 29 patients with Sjögren's syndrome (SS: 13 I degrees-SS and 16 II degrees-SS patients) and 11 patients with rheumatoid arthritis (RA) by two-color flowcytometry using antibodies to antigens serially expressed on the cell surface after activation--interleukin-2 receptor (IL-2R), HLA-DR, T-lineage specific antigen (TLiSA-1) and very late antigen (VLA-1). The early and intermediate activation antigens, DR and TLiSA-1, were significantly increased in the PB of SS and/or RA patients. The proportions of activated T-cells were higher in CD8+ cells than those in CD4+ cells. T-cells expressing IL-2R or VLA-1, which appear in the very early or late stages of activation, were also increased in SS and/or RA patients. The results suggest that activated T-cells in the PB of both diseases might be recruited from continuously activated lymphoid organs, and that the activated cells migrate from PB into target tissues. In addition, the increase of activated T-cells in PB might be one of the causes of the deficient cell-mediated immunity reported in these patients. | |
| 3486833 | Comparison of the arthritogenic properties of dietary cow's milk, egg albumin and soya mil | 1986 | The arthritogenic effect of dietary cow's milk, egg albumin and soya milk were compared in Old English rabbits. The 12-week cow's milk feeding regimen produced the highest incidence of significant joint lesions. Lesions were evident but mild at 5 weeks and the synovitis had resolved by 32 weeks. It is suggested that the transient nature of the synovitis may be due to the development of specific secretory IgA antibodies which were detectable in faecal pellet extracts. Sandy Lop rabbits were less susceptible to the arthritogenic effect than were Old English rabbits. Dietary ovalbumin was less arthritogenic than cow's milk despite high titres of serum and synovial fluid antibodies and immune complexes. The rabbits were 'tolerant' to dietary soya due probably to pre-existing levels of soya protein in their diet. Lewis and Wistar strain rats, CBA, Balb/c and C57/BL6 mice fed on cow's milk for 3 months did not develop serum antibodies or synovial lesions. It is suggested that this allergic synovitis is not a model for early rheumatoid joint disease because of the transience of the lesions and lack of stimulation of rheumatoid factor. It may well, however, be a model for the arthralgia seen in patients with certain food allergies. | |
| 3619138 | [Concentrations of superoxide dismutase (copper and manganese), catalase and glutathione p | 1987 | Antioxidant enzymes assays (CuZnSOD, MnSOD, catalase glutathione peroxidase) have been performed by radioimmunoassay in erythrocytes, platelets and plasma of rheumatoid polyarthritis patients. No difference has been shown as compared with control subjects whatever the therapy or the length of time for which the patients have been affected. If a deficiency in antioxidant enzymes is related to the destruction of joint tissues by free radicals, it cannot be detected in blood tissue elements. | |
| 3148712 | Lymphokine production in rheumatoid arthritis and systemic lupus erythematosus. | 1988 Nov | The production of interferon (IFN) by peripheral blood lymphocytes from patients with rheumatoid arthritis (RA) and of IFN and interleukin 2 (IL-2) in systemic lupus erythematosus (SLE) was compared with that of healthy controls. Patients with SLE showed a significant reduction in IL-2 production compared to controls if the PBL were irradiated before mitogen stimulation. No patient with RA or SLE studied had impaired IFN production regardless of disease activity and the IFN produced was always IFN-gamma in type. We conclude that there is an abnormality in IL-2 production in SLE but there is no abnormality in IFN-gamma production in either RA or SLE. | |
| 3497759 | Natural killer (NK) cells at inflammatory sites of patients with rheumatoid arthritis and | 1987 Jun | NK cell activity and Leu 7+ cells were determined in mononuclear cells (MNC) from patients with rheumatoid arthritis (RA) and IgM rheumatoid factor positive polyarticular juvenile rheumatoid arthritis (JRA). NK cell activity was measured in a 51Cr release assay and the Leu 7 positive cells were enumerated in indirect immunofluorescence. The mean NK cell activity +/- SEM was reduced in MNC from peripheral blood (PB), synovial fluid (SF) and synovial membranes (SM's) of patients with RA, with the values of 19.5 +/- 1.4 (p less than 0.00003), 18.3 +/- 3.1 (p less than 0.009) and 2.9 +/- 0.5 (p less than 0.0003) respectively, compared with 26.1 +/- 1.4 in MNC from the PB of healthy controls. The mean percentages of Leu 7 positive cells in MNC from PB and SF on patients with RA were normal while the mean percentage of Leu 7+ cells in MNC eluted from SM's was significantly reduced as compared to that of MNC from PB of healthy controls (p less than 0.0006). In JRA similar results concerning NK activity and Leu 7 positive cells were found but the number of experiments was too low for statistical analysis. MNC from the SF, in contrast to that of BP and SM, had a significant cytotoxicity against the Raji cell line which is a non-NK cell target. | |
| 2180049 | Anaemia in rheumatoid arthritis: pathogenesis, diagnosis and treatment. | 1990 | The pathogenesis, diagnosis and treatment of the anaemia of chronic disorders (ACD) in rheumatoid arthritis (RA) were reviewed. Causes of anaemia other than ACD frequently present in RA. Decreased iron absorption was shown to be the result of active RA rather than a cause of ACD or iron deficiency. It has been hypothesized that bone marrow iron availability decreases due to decreased iron release by the mononuclear phagocyte system or that the anaemia in ACD is due to ineffective erythropoiesis; these remain controversial theories. Studies considering a decreased erythropoietin responsiveness have not produced consistent results. Erythroid colony growth is suppressed in vitro by interleukins and tumour necrosis factor but their role in vivo in ACD is unknown. The diagnosis of ACD is made by exclusion. Iron deficiency is detected by transferrin, ferritin, and cellular indices after adaptation of their normal values. Treatment of the anaemia consists merely of antirheumatic treatment. Iron administration is counterproductive since iron chelators or exogenous erythropoietin administration might increase erythropoiesis. | |
| 3035735 | [A new basic drug prospidin in the treatment of rheumatoid arthritis. III. Comparative ana | 1987 | A comparative study of the cytostatic drugs prospidin and cyclophosphamide used in equal doses for rheumatoid arthritis (RA) is reported. Clinical and immunologic effects were determined, and the nature and incidence of side effects and complications were compared. Prospidin showed a more pronounced antirheumatic effect, making for a smaller daily requirement of nonsteroid anti-inflammatory agents or hormones in hormone-dependent cases. Unlike cyclophosphamide, prospidin was not associated with severe side effects and complications precluding further use of the drug. Both drugs demonstrated a regulatory effect on RA-associated immunologic disorders. | |
| 1701623 | Correlation of fatty acid composition of adipose tissue lipids and serum phosphatidylcholi | 1990 Nov | To establish the concentrations of micronutrients in serum, fatty acid composition in serum phosphatidylcholine and in adipose tissue, and their correlation with inflammation and disease duration in rheumatoid arthritis (RA), 21 consecutive patients with recently diagnosed disease (mean duration eight months), 21 patients with longstanding disease (mean duration 15 years), and 57 controls were examined. In the patients with RA low concentrations of the essential fatty acids, linoleic acid (18:2) and linolenic (18:3) acid, and high concentrations of total saturated fatty acids, both in serum phosphatidylcholine and in adipose tissue, were found, abnormalities that increased with disease duration. The proportion of 18:2 in serum phosphatidylcholine correlated inversely with such acute phase proteins as orosomucoid and C reactive protein. It is proposed that the decreases in essential fatty acids are related to increased activity in the desaturase/elongation enzymes, increased production of eicosanoids, or metabolic changes secondary to cytokine mediated inflammatory reaction. When the micronutrients were studied it was found that serum concentrations of selenium were lower in patients than in controls, but not those of ascorbic acid, alpha-tocopherol, retinol, folic acid, or cobalamine. Ascorbic acid concentrations tended to be lower in RA, however, and correlated inversely with those of haptoglobin, orosomucoid, and C reactive protein, indicating a relation between the ascorbic acid concentration and the degree of inflammation. | |
| 3649166 | Activation of factor B of the alternative pathway of complement. Assessment by rocket immu | 1987 Jul | During activation of the alternative pathway of complement, Factor B is cleaved into a smaller fragment Ba and a larger fragment Bb. The Ba in plasma was quantitated by one-dimensional rocket immunoelectrophoresis after precipitating plasma B and Bb with 21% polyethyleneglycol. This method was much more sensitive and faster than other technics with which it was compared. Fragment Ba was detected in 22 of 28 rheumatoid arthritis plasma samples and in 12 of 15 systemic lupus erythematous samples but in only 8 of 68 samples obtained from healthy volunteers. The traditional approach of measuring total Factor B for the assessment of activation of the alternative pathway showed values that were even higher than normal in these diseases. The measurement of plasma Ba promises to be a valuable means of assessment of the activation of Factor B and the alternative pathway of complement in clinical disorders. | |
| 3651579 | Viscoelastic properties of whole blood. Influence of fast sedimenting red blood cell aggre | 1987 | Red blood cell (RBC) aggregation is known to be of deciding influence on erythrocyte sedimentation-rate (ESR) and on whole blood viscoelastic properties. The rheological behaviour of blood collected from a control-group with normal ESR is compared to the viscoelastic behaviour of blood collected from two groups with high to very high ESR, whose individuals are suffering from chronical polyarthritis and Morbus Bechterew, respectively. The rheological properties are evaluated by means of an oscillating-flow capillary-rheometer where the viscous (eta') and elastic (eta") component of the complex viscosity (eta) is measured at a constant frequency of 2 Hz. Correcting for the varying hematocrit of the different blood samples according to an exponential equation, the viscoelastic data are found to be elevated in the groups with high ESR. For the viscous properties this is only due to the increase of the plasma viscosity. A correction for the plasma viscosity, however, shows that the viscous properties at low shear- rates (2s-1) are significantly reduced, whereas elastic properties in a range of medium shear-rates (10s-1 to 50s-1) are significantly increased (P less than 0.001, t-test of Student). This result is discussed to be due to the high packing density of the RBC in fast sedimenting aggregates. High packing density reduces the effective volume of the RBC but increases the stiffness of the aggregates. | |
| 1801258 | Incidence of subaxial subluxation in patients with generalized rheumatoid arthritis who ha | 1991 Oct | A retrospective study of 79 patients with rheumatoid arthritis was undertaken to determine the incidence of subaxial subluxation after upper cervical fusions. Fifty-five patients had isolated axial subluxation and underwent atlantoaxial fusion. Twenty-four underwent occipitocervical fusion for atlantoaxial subluxation and superior migration of the odontoid. The two groups were comparable in age, duration of disease, and time to follow-up (atlantoaxial fusion, 65.4 months; occipitocervical fusion, 72.6 months). Fusion rates were comparable: 80% atlantoaxial fusion and 90.1% occipitocervical fusion. Of the occipitocervical fusion patients, 36% developed subaxial subluxation requiring surgery at an average of 2.6 years after fusion. Of the atlantoaxial fusion patients, 5.5% developed subaxial subluxation requiring surgery after an average of 9 years after fusion. No patient with atlantoaxial fusion developed superior migration of the odontoid. Subaxial subluxation requiring surgery developed early in patients after occipitocervical fusion. This was not true of atlantoaxial fusion. Early atlantoaxial fusion seemed to prevent the development of superior migration of the odontoid. When occipitocervical fusion is necessary and early subaxial subluxation is present, longer fusions or occipitocervicothoracic fusion should be strongly considered. |