Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 2192971 | [Incidence and function of Langerhans cells in various corneal diseases]. | 1990 | Using immunohistochemical techniques, we investigated the distribution and frequency of Langerhans cells in corneal buttons obtained from patients who underwent corneal transplantation because of various corneal diseases. The frequency of these dendritic cells was similar to that in the normal epidermis in corneas with epidermalization after severe alkali burns. Numerous Langerhans cells, albeit in smaller numbers, were also present in the central corneal epithelium of patients with keratitis due to infection with herpes simplex virus, keratitis due to herpes zoster virus, bacterial corneal ulcers, corneal scars, corneal ulcers associated with rheumatoid arthritis, and patients with chronic corneal allograft reactions. The presence and persistence of Langerhans cells in diseased corneas may account for, at least in part, a breakdown of corneal immune privilege with a higher rate of rejection episodes after corneal transplantation. Furthermore, it is probable that Langerhans cells as potent antigen-presenting cells may also play an important role in the initiation and the progression of immune responses in various inflammatory corneal diseases. | |
| 2740803 | [Inhibitory action of tiopronin on free radicals]. | 1989 Apr 30 | Free oxygen radicals are involved in many pathological conditions and particularly in the inflammation which characterizes rheumatic disorders. Tiopronin (Acadione), a new basic treatment for rheumatoid arthritis, and sodium 3-aurothio-2-hydroxypropane-1-sulfonate (Allochrysine), the reference treatment for the disease, have been tested in vitro for their ability to regulate the free radicals produced by inflammatory murine macrophages or granulocytes. The production of free radicals was measured using the technique of luminol-dependent chemoluminescence. Results show that tiopronin causes dose-dependent inhibition of chemoluminescence whereas, under the same experimental conditions, sodium 3-aurothio-2-hydroxypropane-1-sulfonate leads to a stimulation in production of the radicals. Taking into consideration the deleterious effects of free radicals in rheumatic disorders, inhibition of the radicals may be considered as forming part of tiopronin's mode of action. | |
| 2568742 | Effect of histamine on the T-cell colony formation of PHA-stimulated cells. | 1989 Apr | The effect of histamine on T-cell colony formation was studied in human peripheral blood mononuclear cells. Histamine inhibited dose-dependently (10(-4)-10(-6) M) the colony formation of PHA-stimulated T-cells. The inhibition was similar in normal controls and rheumatoid arthritis (RA) patients in spite of the fact that in RA the colony formation was significantly lower than in the normal controls. No increase of colony formation was observed at low concentrations (less than 10(-7) M). Impromidine was less effective than histamine, and pyridylethylamine (PEA) was inactive. Cimetidine counteracted the effect of histamine while chlorpheniramine did not. The results show that colony formation may be inhibited through H2-receptors. This action may be of importance in cellular interactions in tissues with high local histamine concentrations. | |
| 3175567 | [Wegener's granulomatosis and autoantibodies against cytoplasmic granules of neutrophils]. | 1988 Aug 20 | According to the literature, autoantibodies against extranuclear components of polynuclear granulocytes (ACPA) are almost exclusively present in sera of patients with Wegener's granulomatosis and their serum concentrations reflect disease activity. When measurement of these autoantibodies was introduced in our routine laboratory, 12 out of the first 70 samples gave positive results by enzyme immunoassay and by indirect immunofluorescence on alcohol-fixed granulocytes. The 12 patients with ACPA proved to have Wegener's granulomatosis. The titers were distinctly higher in patients without therapy than in those under an immunosuppressive regimen. As a control, 250 sera were tested of patients with various disorders such as SLE, rheumatoid arthritis, acute and chronic hepatitis etc. ACPA were detected in 4 individuals (1 patient with acute non A/non B hepatitis, 2 patients with chronic aggressive hepatitis and 1 hemodialysis patient with unclear glomerulonephritis). These results further confirm the diagnostic value of ACPA in Wegener's granulomatosis. | |
| 3133383 | Chromatographic and electrophoretic studies of immune complexes in non-A, non-B hepatitis. | 1988 Apr 8 | Immune complexes isolated from two patients with chronic non-A, non-B hepatitis, one patient with acute non-A, non-B hepatitis and one patient with juvenile rheumatoid arthritis were examined by means of a combined chromatographic and electrophoretic method. Both analyses showed the presence of complexes consisting of IgG, IgM, complement c1q factor and albumin; no antigen constituents were detected. The IgG-to-IgM ratio varied from 1:1 to 4:1, suggesting that one could be dealing with complexes of both IgG-IgM and IgG-IgG types. Moreover, the detectable presence of c1q factor might indicate that such complexes were capable of activating complement. | |
| 3498813 | Human leukocyte antigen (HLA) DR4. Positive association with rapidly progressing periodont | 1987 Sep | The relationship between human leukocyte antigens (HLA) determinants and periodontitis has been examined by several authors without showing any particular pattern. However, no study has investigated the HLA-D determinants, which are generally associated with immune disorders, and rapidly progressive periodontitis (RPP). The HLA profile of 10 RPP patients was compared with that of a healthy control population (n = 120). Although no significant difference was found for HLA-A, HLA-B, and HLA-C, HLA-DR4 of the HLA-D group was found in 80% of patients but only in 38.3% of controls. A high frequency of HLA-DR4 has been reported in rheumatoid arthritis (RA) patients. This finding may be significant in light of previous reports highlighting similarities between RA and periodontal disease. | |
| 3821780 | Cytogenetic effects of penicillamine. | 1987 Mar | Penicillamine (PA), a drug used for the treatment of rheumatoid arthritis induces sister-chromatid exchanges (SCEs) and chromosome aberrations in cultivated mammalian cells. PA in concentrations from 400 micrograms/ml upward induced SCEs and proliferative delay in human blood cultures when added for the last 24 h of the culture period. In V79 Chinese hamster cells SCE induction was found after acute exposure to PA before the addition of BrdUrd and after chronic exposure during one cell cycle in the presence of BrdUrd. The effect of PA on SCE frequencies occurred both after treatment in complete medium and in serum-free medium and was not influenced by the application of an S9 mix. The simultaneous addition of peroxidase reduced the PA-induced SCEs whereas catalase did not show any effect. Chromosome analysis in the first mitosis after PA treatment revealed a significant increase in the incidence of chromosome aberrations and endoreduplication. The results are discussed with respect to the cause and the significance of the observed effects in connection with mutagenicity testing. | |
| 3596967 | Alteration of chloroquine accumulation in human polymorphonuclear leucocytes under inflamm | 1987 | The present study concerns the established antirheumatic drug chloroquine (CQ) in relation to a possible target for drug therapy, the polymorphonuclear leucocyte (PMN). Its primary aim was to find out whether inflammatory conditions would influence the cell association of CQ. After a suitable ligand-binding assay was developed to measure the cellular association of CQ, the normal characteristics of the cellular transport of CQ were determined. The effect of the inflammatory conditions on CQ cell association was investigated with peripheral PMNs from rheumatoid arthritis (RA) patients, and with normal PMNs, in the absence and presence of the soluble cell-stimulators phorbol ester and chemotactic peptide. Under inflammatory conditions the intracellular concentration of CQ is reduced. This decrease is explained by three possibilities which are involved in cell activation: the leucocyte volume expansion, the cytoplasmic or lysosomal pH changes, and the exocytic release of the granules (degranulation). Our results suggest that this decrease will adversely affect the therapeutic effects, if the mode of anti-inflammatory action of CQ is related to the intracellular drug concentrations. | |
| 3530991 | Characterization of circulating immune complexes in heart disease. | 1986 Aug | Circulating immune complexes (CIC) were characterized for the content in IgG, IgA, IgM, C3 and C4 in patients with heart disease. The levels of IgG, IgM and C4 in PEG-precipitates of patients' sera were significantly higher than those found in controls, and the precipitation profile was similar to that of rheumatoid arthritis patients. Differences were observed in the composition of CIC: IgM was highest in association with myocarditis, and C3 predominated in cases of valvular disease. Complex-bound C4 was significantly higher in patients with myocardial infarction which developed pericarditis either early or late in the evolution of the disorder. Antimyocardial antibodies could be detected in sera and in corresponding PEG-precipitates. The bulk of the data suggests that CIC might play a pathogenetic role in various heart diseases. | |
| 3486284 | Interleukin 1 stimulation of synovial cell plasminogen activator production. | 1986 Feb | Addition of human monocyte interleukin 1 (IL-1) to cultured human synovial cells can cause an increase in both cell associated (30-fold) and extracellular (40-fold) plasminogen activator (PA) activity. This increase was inhibited by antibody directed against IL-1 and phenylglyoxal. PA activity could be detected 3 h after the addition of IL-1, continued to increase for 24 h and was dependent on RNA and protein synthesis. The molecular weight of the PA produced from the IL-1 stimulated synovial cells was 55,000 +/- 1,000. Mononuclear cell conditioned media (MCCM) also stimulated synovial cells to produce PA. This stimulation was partly inhibited by anti-IL-1 thus suggesting the presence of appreciable IL-1 activity in MCCM. These results could provide clues as to how immune events are linked to cartilage destruction associated with rheumatoid arthritis. | |
| 3816425 | The properties and turnover of hyaluronan. | 1986 | Hyaluronan (HA) was discovered over 50 years ago but its metabolism and cellular interactions have only recently received detailed attention. HA is synthesized in the plasma membrane by addition of monosaccharides to the reducing terminal. In tissues, it occurs bound to plasma membranes, aggregated with other macromolecules, or as free polysaccharide. Tissue HA enters the bloodstream in significant amounts through the lymph and is rapidly absorbed via a receptor into liver endothelial cells, where degradation follows. HA levels in serum are normally 10-100 micrograms/l, but can be elevated in cirrhosis, rheumatoid arthritis and scleroderma, due either to impaired hepatic uptake or to increased production. Studies on aqueous humour, middle ear secretion, amniotic fluid, lung lavage fluid, urine, skin diseases and cancer have identified other causes of deranged HA metabolism. HA can be visualized on some cell surfaces as a coating impermeable to particulate material. Specific HA binding occurs on lymphoma cell lines, lung macrophages and SV-3T3 cells but, except in synthesis or uptake, the significance of membrane-associated HA is incompletely understood. It has been reported to activate macrophages and granulocytes, protect cells, control cell migration, and cooperate with intercellular matrix in cell detachment; it also plays a central role in growth control, cellular differentiation and tissue morphogenesis. | |
| 1921424 | Antiinflammatory activity of a Ghanaian antiarthritic herbal preparation: I. | 1991 Jul | A boiling water extract from a powdered sample containing Alstonia boonei root bark (90%) Rauvolfia vomitoria root bark (5%) and Elaeis guineensis nut without pericarp (5%) was tested intraperitoneally for its antiinflammatory activity by measuring rat hindpaw edema induced by the subplantar injection of carrageenin in the presence or absence of arachidonic acid. Arachidonic acid increased swelling during the early phase of carrageenin edema. The extract suppressed the late phase of carrageenin edema and both phases in the presence of arachidonic acid. These preliminary results are consistent with a herbal preparation known to be used in the management of rheumatoid arthritis. | |
| 1668766 | Natural killer cell number and activity in remission phase of systemic connective tissue d | 1991 Jul | The proportions and numbers of peripheral blood mononuclear cell markers and peripheral blood NK cell activity were analyzed and correlated in systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and Sjögren's syndrome (SS) patients in the remission phase of the diseases. In comparison to the control data, the number of CD56+ cells was significantly increased in RA patients only; the same held true for double-positive cells, i.e., the alterations did not distinguish various subpopulations of NK cells. NK cell activity was significantly decreased in all the three groups of patients, with the complete lack of correlation between the NK cell number and their activity. It is possible that a significantly diminished NK cell activity in these diseases provokes a compensatory production of nonfunctional NK cells. | |
| 2190022 | [Inflammatory reaction and laboratory tests: interleukin-1]. | 1990 Mar | Recent progress on the study of IL 1 is summarized and depicted, particularly emphasizing the variety of IL 1 function, and the role of other cytokine inducers. While it has been established that IL 1 has a variety of biological functions in vitro and in vivo, some of these functions are not a direct action of IL 1, but may be ascribed to some other cytokines induced by IL 1, since IL 1 can induce IL 6 (as an inducer of acute phase protein), IL 8 (as a neutrophil chemotactic factor), or IL 1 itself. Other important functions of IL 1 in vivo including resistance to bacterial infection, radioresistance and anti-tumor activity are of great interest to determine whether its action is direct or indirect through the induction of other factors, although the importance of IL 1 yet remains. Finally, the bioassay and ELISA assay of IL 1 alpha and IL 1 beta were established. The IL 1 level in serum, amniotic fluids, and synovial fluids from rheumatoid arthritis have been determined and its biological significance has been discussed. | |
| 2159858 | Attachment of IgG to dermal extracellular matrix in patients with fibromyalgia. | 1990 Mar | Deposits of IgG localized to collagen bundles/extracellular matrix components occurred in skin biopsies from patients with primary fibromyalgia (PF). None of these patients demonstrated a positive lupus band test. Control skin biopsies from healthy controls were negative but showed intense reactivity for IgG after collagenase treatment. PF-skin attached both homologous and heterologous serum IgG in indirect immunofluorescence, which may point to a qualitative alteration of dermal matrix components in PF. Skin from patients with systemic lupus erythematosus and rheumatoid arthritis showed a lower dermal fluorescence intensity than in PF patients. The cause of the presence of IgG in dermal tissue from PF patients is unclear. It may be caused by a non-specific attachment of IgG to the extracellular matrix related, for example, to tissue hypoxia and/or increased capillary leakage due to an increased number of mast cells in the PF-skin. | |
| 2099354 | Presence of autoantibody against nasal mucosal cells in patients with Wegener's granulomat | 1990 | We examined whether or not patients with Wegener's granulomatosis have autoantibodies against the respiratory tract and kidney, by using the direct avidin-biotin-glucose oxidase complex method. On immunostaining, the IgG fractions obtained from the sera of 2 patients with Wegener's granulomatosis and of 7 controls were coupled with biotin, and each couple was used as the primary antibody. The serum IgG sample from 1 patient with Wegener's granulomatosis definitely reacted with epithelial cells of the nasal mucosa, with the strongest reaction in basal layer. The sample from another patients with Wegener's granulomatosis showed a similar but weaker reaction. Control sera showed negative reactions in 6 subjects (3 with rheumatoid arthritis, 1 with allergic granulomatous angiitis and 2 normal volunteers) and a very weak reaction in 1 patient with poly-arteritis nodosa. These observations suggest the presence of an autoantibody reacting with some cell components of the nasal epithelium in patients with Wegener's granulomatosis. | |
| 2691151 | Advances in implant arthroplasty in the upper extremity, circa 1988. | 1989 Sep | Artificial joint replacement has paralleled that in the lower extremity during the past 15 years. The ability to replace the shoulder, elbow, and the wrist joint has relieved pain and restored function to the upper extremities of patients with rheumatoid arthritis. Engineering developments have led to improved design, sufficient enough that the implant durability appears to exceed that of the patients' themselves. The kinematics and design of these implants has been under intensive and continuing study since the inception of these procedures. The exact anatomical restoration of the centers of motion and alignment for movement are essential for the success of these arthroplasties. We report here our long-term experience with shoulder replacement arthroplasties in which the success rate in terms of good and excellent results has been 90%. Similar results have been achieved with elbow replacement arthroplasties during a 14 year follow-up. Wrist replacement arthroplasties have also shown high good to excellent results during a nine-year follow-up period. Experience has demonstrated that if infection and wound complications are minimized, the long-term results are excellent in terms of pain relief, movement, and restoration of function. | |
| 2502901 | Misoprostol: a prostaglandin for peptic ulcer. | 1989 Aug | Misoprostol, an E1 prostaglandin analog, protects gastric mucosa against the ulcerogenic effects of a wide variety of noxious agents, including aspirin and nonsteroidal anti-inflammatory drugs (NSAIDs). This synthetic prostaglandin was recently approved by the Food and Drug Administration for use in patients at high risk of gastric ulceration because of chronic NSAID therapy for osteoarthritis, rheumatoid arthritis and related conditions. Side effects of misoprostol are minimal, but the drug is relatively expensive. Protection against gastrointestinal bleeding has not been established. | |
| 2504918 | B cell suppressing and CD8+ T cell enhancing effects of photosensitive dye platonin in hum | 1989 Jul | Platonin is one of the photosensitive dyes of trithiazole pentamethine cyanine. It is used as an effective medicine for rheumatoid arthritis. In our study, platonin suppressed the immunoglobulin (Ig) production of human peripheral blood lymphocytes (PBL) stimulated by Staphylococcus aureus Cowan I (SAC) or pokeweed mitogen (PWM). It also suppressed the PWM induced Ig production of B cells when T cells and/or B cells were pretreated with platonin, respectively. The percentage of CD8+ T cells was increased by platonin. Our results suggest that platonin suppresses Ig production through suppressing B cells and enhancing CD8+ (suppressor/cytotoxic) T cells. | |
| 2912613 | Occipitoatlantoaxial fusion utilizing a rectangular rod. | 1989 Feb | Atlantoaxial subluxation has been treated conventionally by Gallie posterior fusion. This technique, however, has disadvantages such as the frequent occurrence of pseudarthrosis, a high probability of relapse, and the necessity of long-standing strict external fixation until bone fusion. To overcome these problems, posterior occipitoatlantoaxial fusion was performed using a rectangular rod that assures strong internal fixation in 16 patients with atlantoaxial subluxation. The condition was complicated by superior migration of the dens in five patients. Clinical and roentgenographic examinations before and after the operation showed improvements in neurological symptoms and in pain in the neck and occipital region in all patients. Bone fusion was observed in all patients and reduction, performed to the extent possible during the operation, was retained adequately. The present method, which provides strong internal fixation, allows bone fusion and early initiation of rehabilitation with a simple external support of the neck. It also facilitates laminectomy of C1 in patients with associated myelopathy. This procedure, therefore, is particularly effective in patients with marked instability or with rheumatoid arthritis and makes postoperative application of a halo vest or skull traction unnecessary. |