Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 8295174 | Rheumatoid arthritis: anti-Ro antibodies define a non-HLA-DR4 associated clinicoserologica | 1993 Oct | OBJECTIVE: To determine the clinical significance of anti-Ro antibodies in patients with rheumatoid arthritis (RA). METHODS: Cross sectional study of 278 consecutive patients with RA at a single academic rheumatology center. Clinical data were collected without knowledge of the anti-Ro status. Anti-Ro antibodies were detected in coded sera using immunoprecipitation of HeLa cell extracts. RESULTS: Ten (3.6%) of the 278 patient sera were found anti-Ro positive. Age, sex, duration of disease, prevalence of rheumatoid factor (RF), and severity of joint involvement were similar in anti-Ro positive and negative patients. Anti-Ro positive patients had significantly more symptomatic Sjögren's syndrome (SS), leukopenia, hypocomplementemia, vasculitic purpura, and photosensitivity (odds ratio [OR] varying from 7.3 to 26.8). Although SS was frequent in anti-Ro positive patients with RA, secondary SS was not independently linked to any of the associated extraarticular manifestations. Histocompatibility studies revealed the absence of HLA-DR4 in the 8 anti-Ro positive patients available for typing and the presence of DR2 and/or DR3 in 6 of them. CONCLUSIONS: Immunoprecipitating anti-Ro antibodies in serum delineate a clinical, non-HLA-DR4 associated cluster of patients with RA almost as numerous as systemic lupus erythematosus or primary SS. Production of these antibodies seems to precede clinical manifestations and may thus have prognostic implications. | |
| 8075761 | Intra-articular radiation treatment of rheumatoid synovitis of the ankle with dysprosium-1 | 1994 Jun | Intra-articular radiation treatment using dysprosium-165 ferric hydroxide macroaggregate has been performed on eight patients with refractory synovitis of the ankle. Seventy-five percent (six patients) responded well to the treatment, and there were no complications from the injection. Although ankle synovectomy is not often indicated, those patients with rheumatoid synovitis without significant joint destruction respond favorably to radiation treatment. | |
| 7959594 | [Expression of human endogenous retroviruses in rheumatoid arthritis]. | 1994 Jul | The genomes of all organisms, from yeast to humans, contain thousands of endogenous retroviruses (ERVs) and ERVs are transmitted vertically through germ cells as provirus. In recent studies, it has been reported that some ERVs have important immunologic effects and are associated with autoimmune diseases in several animals. However, despite many years of investigation in human, physiological or pathological roles for ERVs remain unknown. In this study we examined whether the pathogenesis of rheumatoid arthritis (RA) was associated with human ERVs; ERV3 and lambda 4-1, which retain open reading frames (ORF) and could make proteins. We demonstrated that ERV3 and lambda 4-1 were expressed on synoviocytes and peripheral blood mononuclear cells (PBMCs) in RA patients by Northern blot analysis. But the expression was indeed heterogenous: RA patients showed various expression levels of ERV3 and lambda 4-1, which were independent of clinical activities and kinds of used drugs. mRNAs of ERV3 and lambda 4-1 were also expressed in PBMCs of healthy volunteers. We detected a 4.2 kb transcript of lambda 4-1 in synoviocytes and PBMCs which was not expressed in placentas, suggesting a unique protein might be produced in synoviocytes and PBMCs compared to placentas. In order to examine regulatory mechanisms for expression of human ERVs, we treated cultured synoviocytes and human kidney endothelial cells (hKEC) with various cytokines. By interleukin-1 beta (IL-1 beta) treatment, ERV3 expression in synoviocytes was not altered, but that in hKEC increased and correlated with IL-6 expression. Although ERV3 and lambda 4-1 expression may not be directly associated with the pathogenesis of RA, the possibility still exists that some of many ERVs, which are integrated into human genomes, may have causative roles in RA. | |
| 8976637 | Androgens as adjuvant treatment in postmenopausal female patients with rheumatoid arthriti | 1996 Nov | OBJECTIVE: To examine the possible beneficial effect of androgens in postmenopausal women with active rheumatoid arthritis. METHODS: 107 women participated in a double blind placebo controlled trial to evaluate the effect of 50 mg testosterone propionate intramuscularly every two weeks for one year. RESULTS: An improvement in ESR, Dutch health assessment questionnaire, and pain was noted. In addition, 21% of patients treated with testosterone fulfilled the ACR improvement criteria after one year, versus only 4% of the placebo group. The treatment was well tolerated. CONCLUSIONS: Testosterone may improve the general wellbeing of postmenopausal women with active rheumatoid arthritis. | |
| 7835841 | Stereological analysis of the synovial membrane in rheumatic disorders: diagnostic value o | 1994 Oct | Quantitative evaluation of nuclear size of synoviocytes was performed on 48 synovial biopsies in various rheumatic disorders: osteoarthritis (n = 10), rheumatoid arthritis (11), and chronic non-specific synovitis (14). Thirteen tissue specimens from non-inflammatory synovial membrane were included as a control group. Using the point-sampled intercepts method, unbiased stereological estimates of volume-weighted mean nuclear volume (nuclear upsilon v) were obtained. A slight increase in nuclear volume was observed in osteoarthritis in comparison with the control group with an overlap in 90% of cases. However, in rheumatoid arthritis there was a significant increase of nuclear upsilon v. Significant differences were found between rheumatoid arthritis and the control and osteoarthritis groups (P < or = 0.001). In biopsies from patients diagnosed as chronic non-specific synovitis the averaged nuclear upsilon v values were between those in osteoarthritis and rheumatoid arthritis with a wide range of data. Similar, but less significant differences were demonstrated between rheumatic disorders when using mean nuclear area. Further analysis of chronic non-specific synovitis patients in combination with nuclear upsilon v estimates as a simple, unbiased, complementary tool are required to better establish the diagnostic value of nuclear stereology in the diagnosis of rheumatic disorders. | |
| 8403590 | An identical twin pair discordant for rheumatoid arthritis and ankylosing spondylitis. | 1993 Jul | Both rheumatoid arthritis (RA) and ankylosing spondylitis (AS) have an increased familial occurrence and each disease is associated with the inheritance of specific HLA antigens. We report a pair of identical twin brothers with discordant disease phenotypes: one developed AS at the age of 26, and the other developed RA at the age of 55. The twins possessed both of the disease susceptibility antigens HLA B27 and DR4. Differences in the twins' environmental exposure are discussed. | |
| 8499738 | [High-dose immunoglobulin therapy of rheumatoid arthritis]. | 1993 Apr | Eight patients suffering from severe rheumatoid arthritis were treated with high-dose human immunoglobulin. Doses of 500 mg/kg were administered intravenously in the morning on 4 consecutive days. Improvement of arthritic symptoms was indicated by a significant decrease of the Ritchie index, the number of swollen joints, and the duration of morning stiffness as well as an increase of grip strength. The improvement lasted up to 4 months. In 2 patients the disease activity increased again after 4 weeks. Investigations of laboratory parameters demonstrated a significant reduction of the acute-phase response. Parallel investigations of immunological parameters revealed a decrease of Il-6 levels in the sera. Therefore, we suggest a decreased activation status of the monocyte-macrophage system and T cells as a possible mode of action of high-dose immunoglobulin therapy in rheumatoid arthritis. | |
| 8009010 | [Systemic manifestations of rheumatoid arthritis: prognostic value of the HLA DRB1 0405 al | 1993 | The aim of the present study was to determine HLA DRB1* genotype by PCR-RFLP analysis in patients with rheumatoid arthritis (N = 122) associated with extra-articular manifestations (N = 24). A significant increased of DRB1* 0405 was found in RA patients with extra-cellular involvement (odd-ratio = 8.33, confidence interval = 1.44 - 56.7). In patients with RA, HLA DRB1*0405 allele might constitute a susceptibility marker of extra-articular involvement such as vasulitis or cardio-pulmonary manifestations. | |
| 1526695 | On the medicinal efficacy of dietetic therapy in patients with rheumatoid arthritis. | 1992 | A controlled trial of 68 patients with rheumatoid arthritis on the medicinal efficacy of a dietetic therapy was made. The employment of a special diet favourably influenced the course of the disease and enabled the dosage of antirheumatic drugs to be reduced, thereby reducing the frequency of side-effects of the drugs. | |
| 7796363 | Spontaneous tendon rupture in rheumatoid hand-successful reconstruction with tendon transf | 1995 Apr | Rheumatoid arthritis is not as common a problem in Taiwan as it is in western countries. Compared with traumatic tendon rupture, tendon rupture in rheumatoid arthritis has its complex and peculiar pathophysiological mechanism which includes chemical erosion by hypertrophic synovium and mechanical attrition by deformed joints and bony spurs. In management, all these pathological foci must be eradicated to prevent recurrent failure. Tendon transfer is a simple and effective method for reconstructing lost tendon function. | |
| 7788151 | Muscle strength in healthy people and in patients suffering from recent-onset inflammatory | 1995 Apr | Neuromuscular function was compared among 20 patients with relatively recent onset (symptomatic period 17 +/- 24 months) rheumatoid arthritis (RA) (experimental group; EG), and 20 age- and sex-matched healthy people (control group; CG). The comparison was repeated after a period of 6 months, when 16 patients had carried out progressive strength training. At baseline maximal grip strength and maximal dynamic unilateral strength of the knee extensors in the EG were significantly (P < 0.05) lower in comparison to the CG. The groups did not differ from each other in maximal isometric strength of the trunk flexors and extensors or the knee extensors. The 6-month dynamic strength training in the EG resulted in significant increases in maximal dynamic strength of the knee extensors (P < 0.001), in isometric grip strength (P < 0.001) and in isometric strength of the trunk flexors (P < 0.05) and extensors (P < 0.05) to the level of the healthy controls. Only minor changes took place in explosive strength and maximal isometric strength of the knee extensors. Erythrocyte sedimentation rate (P < 0.001), Ritchie's articular index (P < 0.01) and modified health assessment questionnaire (P < 0.01) improved significantly during the training period. The results suggest that inflammatory arthritis decreases dynamic and/or isometric muscle strength in selected muscle groups of the body already in the early stages of disease. However, progressive dynamic strength training rapidly increases the neuromuscular performance capacity of the patients even to the level of healthy people without detrimental effects on disease activity. | |
| 8969552 | [Incidence of cancer among Japanese patients with rheumatoid arthritis]. | 1996 Oct | Most follow-up studies of patients with rheumatoid arthritis demonstrate no increase in the incidence of cancer apart from approximate doubling of the incidence of lymphoproliferative malignancies. A consecutive series of 1832 Japanese patients with rheumatoid arthritis seen at the center was studied to determine their cancer morbidity. Overall, 35 cancers were diagnosed in the series from 1982 through 1995 and were not significantly in excess of the expected number. No association was found between rheumatoid arthritis and subsequent development of cancer at any site, either. | |
| 9138821 | [Basic plans for the combined orthopedic treatment of patients with rheumatoid arthritis a | 1996 Oct | The paper focuses on the main principles of multi-modality orthopedic treatment of patients with rheumatoid arthritis (RA) presenting with the lower limbs affection, depending upon stage, activity, as well as particular features of disease course, functional incompetence of the joints and the limb as a whole, with due regard to the patient's volition and psychological status. It is necessary that opportunities for the RA patient's orthopedic rehabilitation be found before declaring him to be an invalid. | |
| 8495259 | Bone metabolism in rheumatoid arthritis; relation to disease activity. | 1993 May | Biochemical parameters of bone metabolism were investigated in 105 ambulant, non-steroid treated patients with RA and compared with parameters of disease activity. Urinary calcium (Ca) and hydroxyproline (OHP) excretions, as parameters of bone resorption and serum alkaline phosphatase (AP), as a parameter of bone formation, were positively related to parameters of disease activity. Serum osteocalcin, another parameter of bone formation, was not related to parameters of disease activity. Patients with active disease (ESR > or = 28 mm and Ritchie articular index > or = 8) had a significant higher urinary Ca and OHP excretion (62 and 42% higher, respectively) than patients with inactive disease. Serum AP and OC were slightly higher (19 and 16%, respectively) in patients with active disease. These results suggest that in RA patients bone metabolism is related to disease activity. In active disease bone resorption seems to be increased more than bone formation, suggesting that prolonged disease activity may contribute to generalized and/or localized osteopenia. | |
| 8220921 | Glomerulonephritis in rheumatoid arthritis. | 1993 Nov | We present data on 10 patients with RA who developed glomerulonephritis which was not related to gold or penicillamine therapy. Although two of these patients had received gold this had been discontinued 13 and 18 yr before the diagnosis of glomerulonephritis. Seven patients presented with nephrotic syndrome and three with proteinuria and microscopic haematuria. Renal histology showed a membranous nephropathy (five patients), mesangial IgA nephropathy (two patients), focal segmental necrotizing glomerulonephritis (two patients) and focal segmental glomerulosclerosis (one patient). | |
| 8574614 | Impact of using stored cells for immunofluorescence detection of antiperinuclear factor on | 1995 Jul | Antiperinuclear factor is as sensitive as (0.75 to 0.80) and more specific than (0.94 to 0.97) rheumatoid factor for the diagnosis of rheumatoid arthritis. Although three groups found similar performance characteristics using the assay technique described by Youinou, lower sensitivities have also been reported. To clarify these discrepancies, we investigated each parameter of the assay, including storage time of the oral mucosa cells used as the substrate. Even when the slides were frozen, titers fell by one dilution within the first week and by two dilutions within two weeks after sampling. This decline seemed related to storage rather than to freezing: slides kept at 4 degrees C yielded an apparent three-dilution fall in titers after one week and were unevaluable after longer storage times. Successive freeze-thaw cycles did not influence results when the assay was done on the day the cells were sampled and fixed. Titers in sera stored at -25 degrees C remained unchanged or decreased by no more than one dilution during the first 18 months but declined thereafter. These data emphasize the need for performing the assay on the same day or, at the latest, on the day after fixation of the slides. That this precaution was taken should be specified in the "Methods" section of articles on antiperinuclear factor detection. | |
| 8316903 | [Analysis of the state of patients with rheumatoid arthritis using a personal computer]. | 1993 Apr | The present study was designed to analyze the state of 203 patients with rheumatoid arthritis (RA) in our clinic using a personal computer. The duration of the disease was significantly correlated with the progression of radiographical stages of RA, indicating that no drugs were able to prevent the progression of joint destruction. Nevertheless, bucillamine, a newly developed anti-rheumatic drug, was found to be effective in some patients who did not respond to D-penicillamine, but not vice versa. Although seropositive RA patients had more severe disease than seronegative RA patients, serum rheumatoid factor levels were not significantly correlated with Lansbury indices in seropositive RA patients. Overall, either the liver function or the renal function was not significantly affected in any category of the patients. Taken together, it is suggested that analysis of the data of the RA patients with personal computer systems provide useful information on the management of the RA patients. | |
| 8689824 | Advantages of an increased dose of MRI contrast agent for enhancing inflammatory synovium. | 1996 Jul | MRI intravenous contrast enhancement of inflammatory synovium has been studied at two different doses to determine to what extent enhancement is dose dependent. 19 patients with clinically active rheumatoid arthritis involving a knee were scanned twice, one week apart, using 0.1 mmol/kg of gadoteridol (ProHance) on the first occasion and 0.3 mmol/kg on the second. Static pre-and post-contrast images together with dynamic images immediately following injection were obtained on a 1.0T scanner. On subjective assessment, 84% of patients showed improved enhancement. 47% showed more enhancing tissue, a clearer delineation of enhancing tissue or both. Objectively, enhancement was increased significantly at the higher dose, as judged by the percentage increase in mean signal intensity within regions of interest plotted over the suprapatellar pouch (1723% v. 1005% enhancement P < 0.05). In practical terms the better visualization of enhancing tissue achieved with higher doses is likely to reduce margins of error in attempts at quantification from MRI scans, particularly of synovial volume, but emphasizes also the need for care and consistency to be exercised in calculating the exact dose of contrast medium to be administered. | |
| 8984946 | Association of complement alleles C4AQ0 and C4B5 with rheumatoid arthritis in Koreans. | 1996 Oct | OBJECTIVE: To investigate the association of complement C4 allotypes with rheumatoid arthritis in Koreans. METHODS: 65 rheumatoid arthritis patients and 255 controls were typed for C4 allotypes and HLA-A, B, C, DR, and DQ antigens. RESULTS: The frequencies of C4AQ0 (32.3% v 14.9%, P < 0.005) and C4B5 (29.2% v 12.2%, P < 0.005) were significantly increased in rheumatoid arthritis patients compared with healthy control subjects. Among rheumatoid patients, the frequency of C4AQ0 was significantly increased in both the rheumatoid factor (RF) positive (27.3%) and the RF negative (66.7%) subgroups. The frequencies of C4B5 and HLA-DR4 were significantly increased only in RF positive subgroup. C4B5 was strongly associated with HLA-DR4, whereas C4AQ0 did not show association with DR4. CONCLUSIONS: In Koreans, C4AQ0 and C4B5 are associated with susceptibility to rheumatoid arthritis, as in the Japanese. C4B5 is strongly associated with HLA-DR4. C4AQ0 is considered to be a DR4 independent risk factor, and a disease susceptibility allele in linkage disequilibrium with C4AQ0 is suggested in Korean patients with rheumatoid arthritis. | |
| 8162008 | [New treatments of rheumatoid arthritis for future use]. | 1993 May | Rheumatoid arthritis is a common autoimmune disorder for which current treatments are unsatisfactory since they usually fail to control joint destruction. Pathophysiological mechanisms are incompletely understood but T-lymphocytes and antigen-presenting cells play a key role. Novel therapeutic approaches are directed to these cell types via the trimolecular T-cell receptor-major histocompatibility complex molecules-peptide (TCR-MHC-peptide) complex and other molecules involved in lymphocyte activation. The goal is to restore tolerance through highly selective immunosuppression to minimize adverse effects. Monoclonal antibodies (Ab) directed against a number of targets are potent tools. Antibodies to CD5, CD7, CD54 and CDw52 act on the overall T-cell population. Antibodies against CD4, CD25 or HLA-DR produce more limited immunomodulatory effects. Most human studies to date are encouraging but not controlled study has yet been published. The most satisfactory therapeutic approach involves restoration of tolerance through inhibition of auto-immune T-cell clones. The existence of these clones has been demonstrated in animal models of autoimmune diseases. Successfully developed, highly specific immunological tools include anti-clonotypic monoclonal antibodies, peptide analogs, and anti-T-cell vaccines. The extrapolation to human disease of insights acquired in animal models and the feasibility of these novel therapeutic approaches in rheumatoid arthritis patients are discussed. |