Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 6606433 | Anti-Fab' antibodies in rheumatoid arthritis. Measurement of the relative quantities incor | 1983 Dec | High levels of anti-immunoglobulins that react with Fab' fragments of IgG have been observed in sera of patients with rheumatoid arthritis (RA). To determine how much of these anti-Fab' antibodies are incorporated within immune complexes (IC), we added 125I-Fab' to sera and then measured the amount of labeled Fab' that could be precipitated by adding polyethylene glycol (PEG). Sera were either maintained at neutral pH during this procedure or acidified (pH 3) to dissociate IC. Acidification permitted the 125I-Fab' an equal chance to compete with other endogenous antigens for anti-Fab' antibodies, once excess hydrogen ion was removed. Quantities of anti-Fab' in sera of 20 seropositive RA patients were greater than in sera of 43 age- and sex-matched healthy controls, as measured by this assay and by a solid phase radioimmunoassay. However, significantly less anti-Fab' antibody was incorporated in ICs in the RA patients' sera. Supernatants from cultured peripheral blood lymphocytes of RA patients also contained relatively more "free" and less "hidden" anti-Fab' than culture supernatants from controls. Thus there appear to be qualitative as well as quantitative differences in the anti-Fab' antibodies synthesized by RA patients. This may reflect different proportions of IgM and IgG anti-Fab' in their sera, differences in the average avidity of these antibodies, or differences in the reciprocal relationships within the idiotypic network that result in release of antibodies by certain antibody-producing cells, but not by clones that produce complementary idiotypes. | |
| 6388965 | Statistical analysis of five immune complex screening assays: patterns of detection in pat | 1983 | A comparative study of four nonspecific screening techniques (direct nephelometry, PEG-C4, PEG-IgG, and radiolabeled Clq binding) for immune complexes (IC) and of a technique specific for the detection of insulin-anti-insulin IC was undertaken in four groups of patients with diagnosis of infectious endocarditis, systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and diabetes. The highest frequency of positive results was given by the PEG-IgG test in RA, the Clq-binding test in SLE, the insulin-anti-insulin IC screening test in diabetes, and the PEG-IgG and Clq-binding tests in infectious endocarditis. Of the four nonspecific tests, the PEG-C4 assay appeared to be the least discriminative, since it failed to show significant differences between any group of patients and the group of controls. Direct nephelometry, PEG-IgG, and radiolabeled Clq binding gave consistently higher results in RA than in other diseases, and in this disease the rates of agreement between these tests were highly significant. Significant agreements between the rates of positivity of Clq binding and PEG-IgG tests were seen in all groups of patients studied. Spearman's analysis of rank showed the best correlations among tests based on similar principles (ie, PEG precipitation), and also a strong correlation between Clq binding and the PEG-IgG test in RA. The PEG-IgG test appears to be a reliable IC screening test for general use with the advantage of not involving radioisotopes. In regard to antigen-specific tests, although their specificity and sensitivity may be high, their results may show no correlation with nonspecific screening tests nor with the presence or absence of clinical or laboratory abnormalities suggestive of IC deposition, as exemplified by the insulin-anti-insulin IC screening test in diabetic patients. | |
| 161065 | Lack of suppressor cell activity in rheumatoid synovial lymphocytes. | 1979 | Lymphocytes were eluted from the synovial tissue of seventeen patients with rheumatoid arthritis (RA) and one with ankylosing spondylitis. In eight of these patients immunoglobulin production by synovial lymphocytes in the presence and absence of pokeweed mitogen was studied. In nine patients T lymphocytes were isolated from the eluted cells, and the T helper and suppressor cell functions were evaluated in an allogeneic co-culture system. Peripheral blood lymphocytes (PBL) from twenty-eight normal donors were also studied for comparison. Immunoglobulin produced by synovial lymphocytes was higher than in PBL of normal donors. However, the stimulation index of synovial tissue lymphocytes was lower. Most of the normal donors had suppressor cell activity in their peripheral blood, whereas in synovial tissue lymphocytes a statistically significant number of patients did not have any suppressor cell activity. In contrast, the synovial tissue lymphocytes showed helper activity not differing significantly from that of the T lymphocytes from peripheral blood of normal individuals. | |
| 7378189 | [Study of the effects of free radicals produced by ultraviolet rays on viscosity of human | 1980 Mar 30 | After having pointed out a method measurement of the synovial Fluid (S.F.) viscosity by means of a white blood cell diluting pipette, obtaining results similar to those of Ostwald's viscometer, the AA have studied the osteoarthrotic S.F. viscosity after Ultra Violet Illumination. The production of Free Radicals induced by U.V., determines a fall of osteoarthrotic S.F. viscosity towards the values found in arthritic S.F..So, the AA, indirectly, confirme the capacity of Free Radicals to induce a S.F. viscosity modification like that provoked by the phlogistic process. | |
| 6324703 | Spontaneous and induced immunoglobulin secretion by synovial fluid B lymphocytes in rheuma | 1984 Apr | The functional properties of B lymphocytes in synovial fluid (SF) from patients with rheumatoid arthritis (RA) were analysed by means of a reverse haemolytic plaque forming cell (PFC) assay. SF mononuclear cells spontaneously secreted IgG, but little IgM or IgA. The SF cells failed to respond to the polyclonal B cell activators pokeweed mitogen (PWM) and Epstein-Barr virus. However, SF B cells cocultured with autologous T lymphocytes from the blood and stimulated with PWM secreted IgG but little IgM or IgA. The PFC responses of blood B cells cocultured with autologous SF T cells in the presence of PWM were low; irradiation of the T cells increased the blood B lymphocyte responses, but the differences were not statistically significant. It is concluded that suppressor SF T cells may be partly responsible for the poor response of SF B cells to PWM. | |
| 6508862 | Increased von Willebrand factor antigen in the plasma of patients with vasculitis. | 1984 Dec | The plasma concentrations of von Willebrand factor antigen (vWF:Ag) were determined in 101 patients who had the following diagnoses: vasculitis 8 patients, systemic lupus erythematosus (SLE) 51, rheumatoid arthritis (RA) 28, asthma 7, hereditary angioedema 7. The greatest mean concentration of vWF:Ag, 469% (normal 100% +/- 50), was observed in patients with vasculitis, often without elevation of the erythrocyte sedimentation rate. The mean concentration of vWF:Ag was also increased in both SLE (277%) and RA (194%). Twenty-four patients (15 with SLE, 6 with vasculitis, 3 with RA) had vWF:Ag concentrations greater than 300%. Four of these patients died within 1 year of the date of the study. Of the 15 SLE patients, 9 had vasculitis and 2 had active glomerulonephritis. The 3 RA patients had severe disease associated with extraarticular manifestations. Elevated vWF:Ag may reflect vascular damage, while markedly elevated levels of vWF:Ag appear to indicate a poor prognosis. | |
| 6360749 | Efficacy and safety of a non-acetylated salicylate, choline magnesium trisalicylate, in th | 1983 | The results of three double-blind, multicentre trials are reviewed to compare the efficacy of acetysalicylic acid (ASA) and a non-acetylated salicylate, choline magnesium trisalicylate (CMT), in the treatment of rheumatoid arthritis. In each trial, patients were randomly assigned to receive comparable doses of salicylate as either ASA or CMT. Mean values for clinical indicators of rheumatoid arthritis (number of painful joints, articular index, number of swollen joints, swelling index, duration of morning stiffness) showed similar or greater improvement among groups of patients receiving CMT, compared to those receiving ASA. In addition, the incidence of gastro-intestinal side-effects was lower among patients receiving CMT. | |
| 790555 | A double-blind cross-over evaluation of ketoprofen and aspirin in rheumatoid arthritis. | 1976 | Thirty rheumatoid patients participated in a 6-week double-blind cross-over assessment of ketoprofen (200 mg daily) and aspirin (3.6 g daily). Regular clinical and laboratory assessments were conducted and revealed that at the dosages employed, the two drugs exerted a statistically comparable therapeutic effect. Side-effects were more frequent with aspirin. Ketoprofen was preferred more often by the patients while the investigator found it acceptable as often as aspirin. | |
| 6374139 | Antibody to the mitotic spindle apparatus: immunologic characteristics and cytologic studi | 1984 Apr | A naturally occurring autoantibody directed at the mitotic spindle polar apparatus (MSA) was detected in sera from 18 patients with defined or evolving connective tissue diseases by routine indirect immunofluorescence on a tissue culture cell. This IgG antibody stained the mitotic spindle poles of dividing tissue culture and tissue section substrates, with staining most prominent on substrates of human origin. With some sera, and cell lines, interphase cells showed isolated nuclear staining. Cytoplasmic staining was not apparent in any sera on any substrate. This specificity was shown to be distinct from tubulin by double fluorescence labeling studies. Patient sera exhibiting only this specificity did not precipitate antigens in rabbit or calf thymus nuclear extracts. However, several patients with systemic lupus erythematosus exhibited anti-MSA in combination with other autoantibodies. This autoantibody may be a useful probe for spindle pole-related structures. | |
| 4117170 | Pain patterns in the rheumatic disorders. | 1972 Oct 28 | Pain patterns vary greatly in the different types of arthritis, from the localized agony without mental overtones in acute gout to the diffuse disorder we call rheumatoid arthritis, where inflammation of many joints, systemic illness, anaemia, anxiety, and depression are usually all present in some degree. Each pain pattern calls for a different therapeutic approach, physical, psychological, and pharmacological. Few patients suffer as much pain and suffering over many years as do chronic arthritics. It is all the more important to instruct them in the essentials of their own treatment. A patient with an occupied and instructed mind usually suffers less than an ignorant and depressed one, fearful of her disease and its complications and of the dark uncertain future that lies ahead. | |
| 7061551 | Peroneal-nerve palsy following total knee arthroplasty. A review of The Hospital for Speci | 1982 Mar | Twenty-three postoperative peroneal-nerve palsies in twenty-two patients were documented in the records of 2626 consecutive knee arthroplasties that were performed at The Hospital for Special Surgery from 1974 through 1980. This is an incidence of 0.87 per cent. The preoperative knee deformities included fourteen flexion contractures, eleven valgus deformities, and five biplane deformities. In five patients a peroneal palsy developed despite a peroneal-nerve release at the time of arthroplasty. Only two patients, who initially had only a motor loss, had a complete clinical recovery. Follow-up ranged from six months to seven years (average 3.1 years). Sensory deficits, initially noted in eighteen patients, had not fully resolved in any patient at the time of writing. Although motor deficits were initially noted in all patients, only 28.6 per cent of them fully resolved. The possible causes of the nerve palsy appeared to be direct traction on the nerve, traction on the surrounding tissues resulting in vascular compromise to the nerve, direct pressure on the nerve from the postoperative dressing, or a combination of these factors. When the palsy is discovered, the suggested treatment is a change to a looser dressing and flexion of the knee. The value of surgical exploration of the nerve in the immediate postoperative period is not known. | |
| 6429303 | Cellular effects of human leukocyte hydrolases III: inflammatory exudate and synovial fibr | 1984 Jun | Experiments were undertaken to determine whether lysosomal enzymes obtained from human polymorphonuclear leukocytes (PMN) might adversely affect the viability of human synovial fibroblasts. The effects of the PMN granule enzymes were additionally determined in the presence of an inflammatory exudate. These in vitro results indicated that as a result of these experimental conditions, the lysosomal enzymes, although present in relatively high concentration, were incapable of cell destruction and could only release cells from their growth surfaces; as could other proteases. However, even this effect was not expressed in the presence of naturally occurring inhibitors widely distributed in body fluids, such as serum; and most importantly which are also present in inflammatory exudates. This was in spite of the relatively high dilution of serum and inflammatory exudate used. So that, in effect, the relatively dilute inhibitors present in both serum and inflammatory exudates prevented the relatively concentrated lysosomal enzymes from exerting any discernible effects on either the cells, or the intercellular substance under these experimental conditions. This possibly suggests that the role of PMN granule enzymes as mediators of the cellular destruction observed in many inflammatory diseases, needs further elucidation. | |
| 508975 | Redistribution of pulmonary blood flow in interstitial lung diseases: the chest radiograph | 1979 Sep | Posteroanterior radiographs of the chest showed enlargement of vessels in the upper lung fields in 18 of 29 patients with interstitial lung diseases, despite normal pulmonary wedge pressures and normal or reduced pulmonary blood volumes. The degree of such redistribution ("diversion") did not correlate either with the severity of pulmonary hypertension observed at cardiac catheterization or with radiologic assessment of predominance of disease at the lung bases. Diversion did correlate with several indices of disease severity: reduction in vital capacity, reduction in diffusing capacity, reduction in pulmonary blood volume and radiographic severity of parenchymal abnormalities. Furthermore, diversion correlated with lung height, a variable which was not statistically related to the other indices of disease severity. Distension of upper lung vessels occurs in interstitial lung diseases as the result of a decreased hydrostatic gradient over which the lung is perfused (decreased lung height), partial obliteration of the vascular bed (decreased pulmonary volume), and, more speculatively, decreased extravascular pressure (increased lung recoil). | |
| 1149549 | In vitro pleural fluid clottability and fibrinogen content. | 1975 Aug | Twenty-three specimens of pleural fluid from 23 patients were examined for quantitative fibrinogen, total protein levels, and for clottability in vitro using the recalcification time. Of the 19 specimens of pleural fluid from patients without loculation, 11 (seven exudates) had no detectable fibrinogen; another 8 (six exudates) had a mean fibrinogen level of 55.0 +/- 10.2 mg percent, and a mean recalcification time of 19.4 +/- 2.6 minutes. The pleural fluids from the four patients with loculation had no detectable fibrinogen. The only fluids containing fibrinolytic activity were from the nonloculated non-fibrinogen-containing group. No correlation existed between pleural fluid/plasma total protein ratios and pleural fluid/plasma fibrinogen ratios. In vitro clottability in this study did not reflect the in vivo tendency for coagulation and loculation. | |
| 6607498 | [National survey on reactive arthritis by the French Society of Rheumatology]. | 1983 Nov | In 1982, 3370 cases of inflammatory rheumatism were declared. The geographical analysis of the distribution of these cases (departmental and regional) suggests that their distribution is not homogeneous throughout the country, with a predominance of psoriatic arthritis in the Midi and the Pyrenees and a predominance of rheumatic pelvispondylitis in Aquitaine. Based on the number of cases declared, we can calculate the distribution of each type of inflammatory rheumatism by age and by sex and the incidence per million inhabitants in each age group. These figures are only indicative, as the data from some departments was incomplete. The calculation of a "criterion of frequency" attempts to equalize this bias. Reactive arthritis (352 cases) represents 10.4 p. cent of all cases of inflammatory rheumatism. This large series collected over one year confirms the clinical data already established. The responsible organisms, by order of frequency, were: Chlamydia trachomatis, Yersinia enterocolitica, Shigella, Y. pseudotuberculosis and Ureaplasma urealyticum. 50 p. cent of these cases of arthritis were classified as reactive on the basis of clinical findings and history and the bacteria was not isolated. The HLA-B27 antigen is present in 68 p. cent of the 302 cases in which it was tested. | |
| 1015288 | [Significance of the fusospirillum complex (Plaut-Vincent angina)]. | 1976 | The results are discussed of a retrospective study of 126 patients by whom an excess of the fusospirochaetal complex was found by direct microscopic examination of tonsillar material. These bacteria are not only found in unilateral ulcero-necrotic tonsillitis, but also in different other tonsillar and general diseases. Vincent's angina appears almost exclusively in young persons, aged from 16 to 25 years; in the adolescence (16-20 years) it forms about 40% of all cases of acute tonsillitis. The fusospirochaetal complex is also often found in chronic tonsillitis and even in morfologically normal tonsils, especially in patients with general diseases like rheumatic arthritis. In one third of the patients with infectious mononucleosis, the complex is present in the tonsillar lesions. The occurrence of Vincent's angina is double as high in women than in men. From our assessments we can conclude that the fusospirochaetal complex has a very low pathogenity, and that it only can grow in patients with a reduced local or general resistance, even without causing local lesions. | |
| 940112 | 6,11-Dihydro-11-oxodibenz [b,e] oxepinacetic acids with potent antiinflammatory activity. | 1976 Jul | A series of 6,11-dihydro-11-oxodibenz[b,e]oxepinacetic acids was synthesized and the antiinflammatory activity determined. Studies on 29 compounds revealed certain structure-activity relationships. In the carrageenan edema test, eight compounds exhibited higher antiinflammatory activities than did indomethacin. Several compounds (2, 9, 14, 22, 25) also proved to have activities superior or comparable to indomethacin in suppressing chronic as well as acute inflammation and carrageenan-induced hyperesthesia. Gastric irritation and lethality rates were less frequently observed with these compounds. | |
| 899299 | [Globulinopathy and hyperuricemia]. | 1977 May | Investigation of the locomotor system of 20 patients is reported. One patient with arthrosis of the hip joint and concomitant osteonecroses had a myeloma. In 5 RA patients the manifestations of secondary amyloidosis are described. Among the patients with monoclonal gamma globulinemia two had polyarthritis and RA could not be excluded in one of them, one had arthritis of the ankle and foot joints, and one had a vasculitis characteristic of polyarteritis nodosa. Among those with polyclonal immunoglobulinemia one had RA, two had arthrosis of knees and hips, and two had ankylosing spondylitis. Furthermore four patients with alpha2-hyperglobulinemia which is important in evaluation of the alpha2-globulin peak in amyloidosis, are described. Three patients had cryoglobulinemia. In 10 patients hyperuricemia was found repeatedly (by the uricase method). Consequently in these diseases it is necessary to search clinically for crystal induced synovitides and arthropathies. The classification of globulinopathies and the method of their determination is indicated and particular investigations are recommended for such diseases, where the activity of the joint process does not correlate with laboratory findings. | |
| 796936 | Ketoprofen - clinical efficacy. | 1976 | Ketoprofen has, over the last few years, undergone extensive clinical trials in many centres throughout the world and these are reviewed. Adequately controlled trials have established its undoubted clinical efficacy as an antirheumatic agent in all the major rheumatic diseases, and have demonstrated therapeutic potency, comparable with other established non-steroidal anti-inflammatory analgesic drugs. | |
| 6224935 | Hepatitis, toxic epidermal necrolysis and pancreatitis in association with sulindac therap | 1983 Jun | We report 2 patients who had serious adverse effects after taking sulindac. One of these patients developed toxic hepatitis and Stevens-Johnson/toxic epidermal necrolysis syndrome which resulted in death. Such fatal reaction to sulindac therapy has not been reported previously. There was temporal relation of ingestion of sulindac to 2 episodes of acute pancreatitis in the 2nd patient, strongly suggesting drug induction. Recent reports of similar side effects with other nonsteroidal antiinflammatory drugs suggest that these drugs may have potentially more serious toxicity than has been recognized. |