Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 6813467 | IV N-acetylcysteine treatment of hematologic reactions to chrysotherapy. | 1982 Jul | Twelve patients with adverse hematologic reactions to chrysotherapy received intravenous N-acetylcysteine (IV NAC) to achieve removal and/or redistribution of gold. Urine gold excretion while on IV NAC doubled that predicted from control measurements. Treatment within 20 days of the last gold injection was associated with better recovery of hematologic parameters. No untoward reactions from IV NAC were encountered. The pathogenesis of gold induced hematologic reactions is examined from both toxic as well as immunological perspectives. | |
| 1155981 | Relationships between rheumatoid cells in culture. | 1975 Jun | Rheumatoid synovial fluid macrophages are compatible with synovial membrane fibroblasts in mixed cell culture. They do not appear to have had any immunological experience recognized by homologous rheumatoid blood lymphocytes. The fusion between macrophages which often takes place in cultures of rheumatoid synovial fluid cells seems to be the result of a cell membrane change between contiguous cells rather than to the release of of a 'fusion factor'. Other combinations and manipulations of the rheumatoid cell population may yet prove fruitful in the search for cell-associated phenomena important in the pathogenesis of rheumatoid disease. | |
| 6454418 | Avidity indices of anti-IgG antibodies in diseases. | 1981 Jun | Anti-IgG antibodies were detected in 69% of 235 sera tested. A simple and reproducible method was developed to measure the avidity index of anti-IgG antibodies. It was found that subjects with systemic lupus erythematosus and progressive systemic sclerosis and normal controls had antibodies with lower avidity index than those with rheumatoid arthritis and Down's syndrome. The difference in avidity seems to be disease specific. Avidity does not correlate with antibody titers, but all sera with high avidity antibody are positive in the latex test. The anti-IgG as measured by this method was shown to be mainly of IgM class. The avidity indices of whole sera and purified IgM fractions were similar. | |
| 6605009 | [T-cell kinetics and course of humoral immune response following subcutaneous immunization | 1983 | Revaccinations with tetanus toxoid fluid vaccine were performed including different groups of volunteers (aged, diabetics, rheumatics). The kinetics of T-cells were demonstrated by rosette technique in a period of 63 days after revaccination. The content of tetanus antitoxin was estimated by the mouse standard neutralization test. T-cell kinetics were different in the investigated groups, probably influenced by age, illness, therapy etc. Only those patients among the aged who were in bad health showed a relatively low humoral booster reaction. | |
| 562303 | New trends in psychosomatic research. | 1977 Aug | Advances in physiological psychology and neuroendocrinology, together with epidemiological studies, have added new dimensions to psychosomatic research. Psychological influences still are accepted as exacerbators or trigger mechanisms, if less often as causes. Theories of psychosomatics which connected specific personality profiles with specific psychosomatic illnesses have lost favor, and multifactorial explanations, which include heredity, environment, social class, life stress, endocrines, brain areas, neurohormones, and immunological mechanisms, are new areas of research. Research methods have become more sophisticated scientifically, particularly in the selection and size of samples tested, and the variety of situations investigated. Psychological reactions to illness in general, terminal disease, and death, and psychological experiences of pain, in addition to variable effects of psychotherapeutic methods and psychotherapists' personality, are identifiable but unquantified influences which seem acceptable as contributors to, if not causes of, psychophysiological disorders. | |
| 66627 | Endogenous activation of latent collagenase by rheumatoid synovial cells. Evidence for a r | 1977 May 5 | To elucidate the mechanism of synovial damage in rheumatoid arthritis, we studied the activation of latent collagenases released from adherent rheumatoid synovial cells in culture. Latent enzyme was not complexed with alpha2 macroglobulin, the prinicpal proteinase inhibitor in serum, and could be activated by trypsin in the presence of alpha2 macroglobulin if sufficient proteinase was added to saturate inhibitor. Latent collagenase bound half as effectively to collagen fibrils as active enzyme. Plasmin was a threefold better activator of latent enzyme than trypsin and could be generated by addition of plasminogen to synovial-cell cultures. Production of both collagenase and plasminogen activator was inhibited by dexamethasone (10(-9) M). These studies emphasize in importance of control of activation in regulation collagenase activity, It is likely that rheumatoid synovium produces both latent collagenase and plasminogen activator; plasmin is activated from its zymogen, plasminogen, present in inflamed tissues, and in turn activates collagenase. | |
| 947641 | Selective inhibition of lymphocyte responsiveness to phytohaemagglutinin in patients with | 1976 Mar | The influence of synovial fluid from four patients with Reiter's syndrome on lymphocyte responsiveness was studied. On synovial fluid was found which specifically depressed the responsiveness of lymphocytes from patients with Reiter's syndrome to the non-specific mitogen phytohaemagglutinin (PHA). The ratio of the responsiveness of lymphocytes cultured in the presence of foetal calf serum (FCS), compared to those incubated in the Reiter's synovial fluid, was used as a measure of the depression induced by the Reiter's synovial fluid. The mean ratio for eight normals stimulated with PHA was 0-70 (range 0-35-0-96), while for eight patients with Reiter's syndrome, it was 0-13 (range 0-07-0-19). Similar studies done with concanavalin A (con A) showed no difference between lymphocytes from normals (0-73) or patients with Reiter's syndrome (0-67). Chromatography of the Reiter's synovial fluid on a Sepharose 4-B column resulted in the separation of three major fractions, one of which exhibited the inhibitory activity. When this active fraction was absorbed with Reiter's lymphocytes, a loss of the inhibitory activity of the fraction was seen. A similar absorption with normal lymphocytes had no effect. These studies demonstrate that a factor present in the synovial fluid of a patient with Reiter's syndrome reacted specifically with lymphocytes from patients with Reiter's disease and not with lymphocytes from normals. The interaction of this factor with lymphocytes from patients with Reiter's syndrome inhibited the responsiveness of these lymphocytes to PHA but not to con A. | |
| 7035034 | Suppression of in vitro antibody response of human peripheral blood lymphocytes by a heat- | 1981 Jun | When fresh autologous serum was added to normal human peripheral blood lymphocytes (PBL), it suppressed greater than 90% of the in vitro anti-SRBC response of these cells. Heating the serum for 30 min at 56 degrees C reversed this suppression, Serum from a patient with rheumatoid arthritis and circulating immune complexes had no suppressive effect on the anti-SRBC response of normal human PBL, but serum from patients having the same disease, without circulating immune complexes, did suppress over 90% of the plaque-forming cell response. Serum from an agammaglobulinaemic patient was also suppressive. Addition of serum from patients with congenital deficiencies of C2, C3, C5 and C8 also has a suppressive effect. Absorption of normal serum with immune complexes markedly decreased levels of C1 and C4, and also reversed the suppressive effect of this serum. These data suggest that a heat-labile factor in normal human serum which can be absorbed by immune complexes suppresses the antibody response to a T-dependent antigen. Other immune suppressors found in normal human serum are heat-stable or do not suppress in the presence of normal serum proteins. Thus the suppressive protein described in these studies may be unique. It is possible that either C1 or C4 or both may play a role in the suppression noted here. | |
| 186966 | [Serodiagnosis of rheumatic disorders]. | 1976 Aug 15 | For the serological diagnostics of the diseases caused by Streptococcus pyogenes (A-streptococci) and secondary diseases (rheumatic fever, acute glomerulonephritis) in the first place the anti-streptolysin reaction is at disposal. In the judgment age is to be taken into consideration and the proportion of the inhibitor in the serum is to be removed by absorption with dextran sulphate. Reliable evidence is to be expected only by the course of the titre. If possible, the antistreptolysin reaction should be supplemented by a second reaction, for which purpose the anti-desoxyribonuclease B-reaction is to be recommended. For supporting the diagnostics of the rheumatoid arthritis at least 2 reactions should be performed, i. e. a haemagglutination test (e. g. after Podliachouk-Harboe) and a particle agglutination test (latex fixation reaction). By means of them the rheumatoid factors (above all antibodies of the IgM-class against gamma-globulin) are proved. The haemagglutinations connect a high specifity with a more insignificant sensitivity, in the particle agglutinations the reverse is the case. Apart from the serological tests mentioned here other proved reactions are mentioned. Besides, data on the valuation of the results and their diagnostic importance are in this paper. | |
| 7356892 | Metabolism of salicylate during chronic aspirin therapy. | 1980 Jan | 1. The effects of chronic administration of aspirin in therapeutic doses (3.9 g/day) on plasma and salivary salicylate levels were studied in eight subjects. 2. The urinary excretion profile for free salicylic acid and metabolites of salicylate were examined. 3. Plasma and salivary salicylate levels declined significantly after peak levels were achieved between days 3 and 10. 4. The decline in plasma and salivary salicylate levels may be due to an induction of a metabolic pathway such as salicylurate formation (Furst, Gupta & Paulus, 1977). Only the mean fraction of salicylate excreted as salicylurate appears to increase with time during the present study, although the change was not significant statistically. 5. The decline in plasma and salivary salicylate levels during chronic therapy may lead to an apparent 'tolerance' of some rheumatoid patients to aspirin. | |
| 6412349 | Frequency and clinical significance of anticentromere and anti Scl-70 antibodies in an Eng | 1983 | To determine the clinical significance of anti-centromere (ACA) and anti Scl-70 antibodies in an English population with connective tissue diseases, we examined the sera of 150 patients, including 40 with systemic sclerosis (SS), who were prospectively studied on the same clinical protocol in our connective tissue disease clinic. ACA was present in 44% of the CREST patients as opposed to only 12% of those with SS and diffuse skin involvement. Only two patients without SS had ACA. Anti Scl-70 was detected in 20% of the patients with SS and only two of those with other connective tissue diseases. We confirmed the specificity of these antibodies for SS. Either anti Scl-70 or ACA was present in half the patients with SS and their presence may represent a useful aid to diagnosis of this disease in patients presenting with Raynaud's phenomenon or an undifferentiated connective tissue disease. While less than half the CREST patients had ACA, this antibody appears to identify those patients within the CREST variant with skin involvement confined to sclerodactyly as opposed to those with acrosclerosis. These patients, however, did not differ in the degree of visceral involvement. | |
| 6242337 | Autologous mixed lymphocyte reactions in rheumatoid arthritis. Characterization of stimula | 1983 May | Reduced lymphocyte responses were detected in the peripheral blood (PB) of patients with rheumatoid arthritis (RA) and juvenile rheumatoid arthritis (JRA) in the autologous mixed lymphocyte reaction (AMLR) using irradiated non-T cells as stimulator cells and T cells as responder cells. The AMLR responses of synovial tissue (ST) and of synovial fluid (SF) lymphocytes were normal. Dendritic cells both from the peripheral blood (PB), ST and SF were very potent stimulators in the AMLR and in vivo activated, HLA-DR positive T cells both from ST and SF were able to stimulate autologous PB T-cells in both RA and JRA patients. A rabbit antiserum produced against a fragment comprising the variable heavy (VH) chain region of an IgG3 human myeloma protein (Kup) reacted with peripheral blood T cells and inhibited the AMLR responses. Thus, VH antigens are integral parts of the T cell receptor for autologous HLA-DR (Ia) antigens. | |
| 6619516 | Pseudorheumatoid nodules in an adult. | 1983 Fall | The finding of soft tissue lesions that present as rheumatoid nodules in a patient with no history or symptoms of rheumatoid arthritis or rheumatic fever sets the stage for a variety of differential diagnoses. One of the diagnoses that must be considered is the pseudorheumatoid nodule, which is rarely found in the adult. This article deals with one such case. A review of the literature is discussed along with a description of the remaining diagnoses from which it is distinguished. In this particular case, the patient presented lesions typifying those of rheumatoid nodules with the absence of any constitutional symptoms. Follow-up to date shows no signs of rheumatoid arthritis, although the literature does suggest the possibility that these lesions may be the precursor of the disease. | |
| 807224 | Clinical and objective assessments of naproxen through 5 years of clinical experience. | 1975 Feb | D-2-(6'-Methoxy-2'-naphthyl)-propionic acid (naproxen) is a relatively well-known non-hormonal anti-inflammatory agent useful for long-term usage. The efficacy of naproxen in controlling the signs and symptoms of rheumatoid arthritis had been uniformly demonstrated in an extensive series of international double-blind trials lasting from a few weeks to four months. This paper deals with our experience with naproxen after 5 1/2 years of continuous usage. During this period of time ten different studies were conducted at the Rheumatology Departments of the General Hospital, Mexico City. The efficacy and tolerance of naproxen have been studied in the following indications: rheumatoid arthritis, osteoarthritis, acute and chronic gout and non-articular rheumatism. In these studies the usual clinical methods were used and some special objective methods were utilized in some others, for example, enzymology, scintigraphy, histopathology and arthroscopy. Some of these studies were comparative ones vs. some other well-known non-steroidal anti-inflammatory compounds such as aspirin, indometacin and ibuprofen. During the last 5 1/2 years 877 patients with different theumatic conditions were treated and observed in our Department and in the private practice. In 365 patients with rheumatoid arthritis excellent results were obtained in 34%, good in 36%, fair in 24% and negative in only 6%. In 358 patients with osteoarthritis excellent results were obtained in 28.2%, good in 46.6%, fair in 20% and negative in 5.3%. In 15 patients with acute gout using naproxen as exclusive therapy, excellent results were observed in nine patients very good in four, and unsatisfactory in only two cases. In 35 patients with chronic gout excellent results were observed in 22, good in 11 and negative in two. 93 patients out of the 877 studied since June 1969 used naproxen continuously as anti-rheumatic therapy for more than one year; 33 for more than two years; four patients for more than three years and five of the patients in a few days will complete 5 1/2 years. The side effects of the compound observed during this long period of time in these 877 patients on the g.i. tract, CNS, etc., although present in a limited number of cases (in less than 10% of total number of patients) were definitely less than those observed usually during anti-inflammatory therapy. | |
| 3876082 | Palindromic rheumatism and Felty's syndrome. | 1985 Sep | The coexistence of palindromic rheumatism and Felty's syndrome in a single patient is described. It is likely that these two manifestations are linked with the patient's possession of HLA-DR4 and that they are prodromata of the patient's eventual development of rheumatoid arthritis. | |
| 6193628 | Ultrastructure of synovial changes in rheumatoid disease and in seronegative inflammatory | 1983 | Synovial tissue has been examined by electron microscopy from patients suffering from either sero-positive or sero-negative inflammatory arthropathies to allow direct comparison of the ultrastructural changes found in these groups and to confirm and extend observations previously made in a smaller group of sero-negative arthropathies. Both groups have been compared with material from healthy controls. The sero-positive group comprised 13 cases of 'definite' or 'classical' rheumatoid arthritis. The sero-negative group consisted of 9 cases of arthritis secondary to Crohn's disease (3); Reiter's syndrome (2); Whipple's disease (1); Behcet's disease (1); Wegener's granulomatosis (1) and ankylosing spondylitis (1). The control tissue was obtained from 6 non-arthritic subjects undergoing surgery for non-inflammatory conditions. Confirmation was obtained of changes previously reported in subcellular organelles, especially in synovial B cells, in all forms of inflammatory arthritis as compared with controls. Attention is now drawn to other intracellular changes in B cells and intermediate cells which included: a marked increase of intermediate filaments and microfilaments: and proliferation of pinocytotic vesicles and rough endoplasmic reticulum. These changes were often accompanied by the presence, in the immediate environment of these cells, of extracellular microfibrillary masses but little or no accumulation of intermediate filaments. It was confirmed that synovial A cells were reduced in number but showed changes suggestive of increased phagocytic activity and also exhibited proliferation of cytoskeletal elements. Differences in these structural changes between sero-positive and sero-negative arthritis were of degree rather than of kind and no 'specific' or diagnostic differences were observed between the various forms of seronegative athropathies. The possible significance of the structural changes observed is discussed. | |
| 6161325 | [Sharp's syndrome. Clinical, immunological and nosographic aspects]. | 1980 Dec 15 | LE cells, ds-DNA antibodies (radioimmunoassay), antinuclear antibodies (ANA) by indirect immunofluorescence (IFI) and anti-ENA antibodies have been sought in 150 clinical cases observed over a 5-year period in the Rheumatology Division of Bari University. For the latter, three parallel techniques were adopted on each serum, each completed by RNA-sensitivity assay for the demonstration of anti-RNP, i.e. IFI, passive haemoagglutination (PHA) and controimmunoelectrophoresis (CIE). The series included systemic lupus erythematodes (SLE), 30 cases; rheumatoid arthritis (RA), 30 cases; progressive systemic sclerosis (PSS), 12 cases; unclassified connective tissue disease (UCTD), 8 cases; mixed connective tissue disease (MCTD), 7 cases; Sjögren's syndrome (SS), 4 cases; dermatomyositis (DM), 3 cases; overlap syndromes (PSS-SLE, SS-SLE), 2 cases; rheumatological and internal miscellanea, 54 cases, LE cells and ds-DNA antibodies were found exclusively in SLE; the anti-ENA were found in various groups of diseases, while the anti-RNP were only demonstrated in the 7 MCTD and in some SLE. Of the three techniques for demonstrating anti-ENA, the PHA proved most sensitive and CIE most specific, whereas IFI was considered most suitable for clinical screening. The clinical aspects of the 7 MCTD faithfully followed the disease picture described by Sharp, but some overlap-syndromes and the unclassified connective tissue diseases did not present anti-RNP. It is also pointed out that nephropathy is not rare in MCTD and that the clinical course of the disease is not always benign. To conclude, it is considered that MCTD merits nosographic autonomy, but further investigations are recommended for more exact nosographical typing of connective tissue diseases. | |
| 4008000 | Reduced zinc in peripheral blood cells from patients with inflammatory connective tissue d | 1985 Jun | By the use of the nuclear microprobe technique, the concentrations of zinc in isolated erythrocytes, platelets, and granulocytes were measured in patients with rheumatoid arthritis, other inflammatory arthritides, and scleroderma. Markedly reduced cellular zinc values were found compared to those measured in healthy subjects. No relation was found to inflammatory activity or disease duration. Plasma zinc was reduced in the majority of the patients and was negatively correlated to the inflammatory activity estimated by ESR and serum orosomucoid. No relation was found between total zinc values in plasma or cells or disease duration. Corticosteroid therapy was instituted in a number of the patients with inflammatory arthritides and induced a significant elevation of total zinc in all cell types, although normalization was not achieved. Plasma zinc values remained unchanged during the treatment. | |
| 156026 | Long term chrysotherapy: incidence of toxicity and efficacy during sequential time periods | 1979 May | The frequency and incidence of certain forms of toxicity in patients receiving long term chrysotherapy for rheumatoid arthritis have been reliably calculated for defined, sequential time periods. Ninety-four patients who received 100 courses of gold therapy for a total treatment period of 134.4 patient-years were followed at one gold therapy clinic. For the period of 0--3 months, the incidences of rash, mouth ulcer, and proteinuria were 9.8, 4.0, and 1.8 episodes per 10,000 patients/month, respectively. However, the incidence of these forms of toxicity decreased progressively in subsequent time periods of continued chrysotherapy. No similar decrease was noted in the incidence of thrombocytopenia, but it would appear that the methodology used in monitoring significantly affects the incidence of clinically important thrombocytopenia. No predictive correlates could be determined for patients who had gone into sustained remission. However, the data strongly suggest that patients who improve within 6 months may continue chrysotherapy for at least up to 3 years with an increasing margin of safety for mucocutaneous and renal toxicity. | |
| 984910 | Familial Felty's syndrome. | 1976 Jun | A family is described in which the mother and 2 of the 5 children had Felty's syndrome, a pattern of inheritance suggesting a dominant defect and one which has not previously been reported. The family is also of interest in that the other sib had rheumatoid arthritis. |