Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 775086 | Double-blind study of Naproxen in osteoarthritis of the knee joint. | 1976 Mar | To determine the effectiveness of naproxen in the treatment of osteoarthritis of the knee joint, a double-blind cross-over study against placebo was carried out on 61 patients. Statistically significant positive therapeutic effects were observed, including analgesia, increased mobility, decrease in joint swelling and improvement in activities of daily living. Side effects were infrequent, mild and disappeared rapidly. | |
| 300023 | Effect of sodium salicylate on human and mouse granulopoiesis in vitro. | 1977 Jan | Sodium salicylate inhibited generation of granulocyte and macrophage colonies when added to soft agar cultures of mouse or human bone marrow cells (CFUc) containing colony-stimulating factor (CSF). This effect was dose-dependent with over 90% inhibition at 48 mg%. The salicylate effect was not decreased with increasing concentrations of CSF, but inhibition was reversed when salicylate-treated CFUc were washed with drug-free medium before plating. CSF production was not inhibited by salicylate. | |
| 6171508 | Interference of beta 2-microglobulin specific autoantibodies with EA-binding of human peri | 1981 Oct | The effect of anti-beta 2 m-specific autoantibodies was investigated on the FcRs of human PBMCs. Anti-beta 2 m autoantibodies inhibited the FcRs of the lymphocyte subpopulation detectable by rosetting with EA(hu). On the contrary, when EA(ox) indicator system was used, in the majority of the cases an enhancement of EA rosette formation was detected. Using separated lymphocyte subpopulations we found that the binding of anti-beta 2 m autoantibodies increased the number of FcR+ non-B-cells and inhibited that of B-lymphocytes. | |
| 2935159 | Partial characterization of synovial fluid nucleotide pyrophosphohydrolase. | 1985 Dec | Synovial fluid adenosine triphosphate pyrophosphohydrolase, an enzyme which manifests increased activity in chondrocalcinosis and osteoarthritis, was partially characterized in synovial fluids from 41 patients who had a variety of arthropathies. Activity was found to be a soluble, heat labile, and divalent cation-dependent nonspecific nucleotide pyrophosphohydrolase with pH optimum 9.0-9.5. | |
| 1084006 | [Xeroradiography in rheumatology]. | 1976 Apr | Xeroradiography is a new and simple radiodiagnostic technique that makes it possible to analyse on one positive film not only the bone tissue but also the neighbouring structures (ligaments, muscles, fatty tissue, the vessels, and the skin). On the basis of about 100 examinations, the authors indicate the value and the limitations of the technique in rheumatological practice. Because of the technical aspects of the technique, the dorsolumbar spine and the facial massif are poorly visualized. The swamping of the contrast also limits its use for cases of demineralization (osteoporosis). On the other hand, the information provided by the technique in cases of Paget's disease, articular or para-articular calcification, pathological conditions of the tendons (rupture, calcification), and bone tumours appears to be of great value. The early diagnosis of cases of inflammatory rheumatism and the surveillance of Silastic prostheses used in surgery on rheumatic hands are also facilitated. | |
| 1065802 | [Evaluation of the effects of some non-steroid anti-inflammatory agents on the gastric muc | 1976 Jul 7 | 300 mg/day phenylbutazone, 210 mg/day indomethacin, and 600 mg/day pyrasanone were administered for 14 days to three randomised groups of patients respectively, consisting of a total of 76 subjects with various forms of non-infectious inflammation (osteoarthritis, fibrositis, rheumatoid arthritis, gout, phlebitis), in a double-blind trila designed to determine the activity of the three drugs and their tolerance. In 36 cases, gastroscopy was performed before and after the treatment. On the basis of doses that were equivalent as far as their anti-inflammatory effect was concerned, epigastric pain and pyrosis were noted in about 31% of the series, though no significant difference could be made out between the three drugs. Gastroscopic evidence of erythema (8 cases), multiple erosion (2 cases), pomphoid gastritis (1 case), and duodenal ulcer (1 case) was obtained in subjects treated with phenylbutazone or indomethacin, and of erythema only (1 case) after pyrasanone. No relation could be established between the clinical symptoms and the gastroscopic findings. | |
| 6967743 | [Significance of the spontaneous rosette formation test with mouse erythrocytes in the com | 1980 Jul | Some mechanisms were studied that are concerned with mouse red blood cell rosette formation by human peripheral blood lymphocytes. Lymphocytes rosetting with mouse red blood cells (EM--RFC) were identified by the test similar to the E-rosette formation technique. EM--RFC accounted for 7.7+/-0.48% in the peripheral blood of healthy donors. It has been established by stepwise selective inhibition of Fc- and C3-receptors and surface immunoglobulins that human Fc- and C3-receptors do not take part in rosetting with mouse red blood cells, whilst surface immunoglobulins, particularly IgM or near-located structures, are implicated in the reaction of lymphocytes with mouse red blood cells. Multiple analysis of lymphocyte receptors has been performed in patients with different immunologic disorders. A significant decrease in the EM--RFC level was found in patients with rheumatoid arthritis. In patients with dys-and hypoimmunoglobulinemia, the content of EM--RFC varied depending on the severity of immunity B-system injury and correlated well with the cells bearing surface immunoglobulins. The drastically increased number EM--RFC (up to 34.6--78%) was recorded in the blood of patients with chronic lympholeukemia. These findings served as basis for applying EM--RFC detection for diagnosing various forms of lympholeukemia. | |
| 6235997 | Comparison of pathologic and normal sera by immune complex determination: five disease gro | 1984 Sep | Pathological (190) and normal (33) sera were tested for their content of circulating immune complexes (CIC) by a battery of 13 assays performed in 11 laboratories. Statistical processing was done both by pooling all pathological samples and by extracting those falling into well-defined disease groups, i.e., rheumatoid arthritis, diabetes, lupus, melanoma, and glomerulonephritis. Highly significant correlations between methods--taken two at a time--for each disease differed in proportion (ranging from 6 to 30%) and in the pattern displayed on a checkerboard. Disease-linked patterns were also found when a function maximizing discrimination between pathological and normal samples was derived by combining the information from all methods. Here the order and the weight attributed by the computer to the methods differed for each of the disease groups. Taken together these results are interpreted as an indication that all assays may not determine the same classes of CIC, and thus vary in sensitivity depending on the prevailing properties of the complexes present in the serum, which in turn may depend on the etiology, pathogenesis, and stage of the disease. | |
| 1215899 | [The complement system]. | 1975 Sep 13 | The complement system may be activated by at least two different pathways: the clinical pathway involving C1, C4 and C2 and the alternative pathway involving properdin, C3, factor B and factor D. The classical pathway can be activated by antigen antibody complexes, while the alternative pathway can be activated by other substances such as natural polysaccharides. Both pathways lead to an activation of C3 and of the last complement components (C5 to C9). Congenital defects of the complement system have been described for several components. Some of these defects are relatively well tolerated, but others, such as C3 deficiency, lead to increased susceptibility to bacterial infections. Acquired complement defects are frequently observed in association with several diseases. Usually they are characterized by an increased level of complement components involved in the classical pathway and therefore reflect activation by antigen antibody complexes. Such changes may be systematic, as in lupus erythematodes, or localized to some biological fluids such as synovial fluid in rheumatoid arthritis. In some renal diseases the complement profile suggests activation of the complement system by the alternative pathway, and this may reflect a different pathogenesis. | |
| 6165073 | Isolation and characterization of intermediate complexes and other components with common | 1981 | Fractions with affinity for heat-denatured human IgG (HDG) were isolated from four sera that contained intermediate complexes (IC). These IC fractions contained part of the 75 IgG, all IC, and part of the rapidly sedimenting complexes (RC) found in the sera. The IC consisted of IgG1 or IgG3 and the RC of IgG and IgM with kappa and lambda light chains. The IgG in the IC fractions contained an abnormally large amount of neuraminic acid. No correlation between IgG subclass or content of neuraminic aid and complex formation was found. There were indications that the formation of IC was not only the result of self-association of IgG molecules with anit-gamma-globulin activity. Specific rabbit antisera were prepared against two of the IC fractions. Affinity chromatography wtih immobilized IgG and F(ab')2gamma from these antisera confirmed the presence of common antigenic determinants in the sera. These determinants occurred mainly in 7S components in one individual, in IC in one and in RC in another. Only a minor part of the serum components with affinity for HDG contained the determinants. RF activity was found in the components that lacked and in those that contained the common antigenic determinants. | |
| 1057506 | [Plasmocytoma, alkylating agents, and acute myeloid leukemia (author's transl)]. | 1975 Sep 26 | Two cases of the development of acute myeloid leukemia (AML) after treatment with alkylating agents are reported. In Case 1, melphalan and then cyclophosphamide had been given for multiple myeloma. 46 months after onset of cytostatic treatment AML occurred, as confirmed cytochemically and by qualitative determination of urinary lysozyme. In Case 2, cyclophosphamide had been given for rheumatoid arthritis. After a latency of 34 months 'smouldering leukaemia' developed with an atypical monocytic leukaemic cell population. In a third case, multiple myeloma and monocytic leukaemia developed synchronously. The causative role of melphalan and cyclophosphamide in the development of AML seems securely established. Despite the risk of alkylating agents in the treatment of multiple myeloma or Hodgkin's disease causing AML, they should not be replaced, as other drugs have been shown to be less beneficial. On the other hand, alkylating agents should be used with great caution in the treatment of non-malignant diseases. | |
| 77370 | Acid hydrolases in monocytes from patients with inflammatory bowel disease, chronic liver | 1978 May 20 | A sensitive technique was used to estimate two acid hydrolases--N-acetyl-beta-glucosaminidase (N.A.G.) and beta-glucuronidase (B.G.)--produced by peripheral-blood monocytes. Enzyme levels were measured after in-vitro incubation of monocytes with or without stimulation by zymosan and endotoxin. Compared with controls, enzyme production and release in inflammatory bowel disease, chronic liver disease, and rheumatoid arthritis were markedly raised. It is suggested that various stimuli, including immunological ones, may be responsible for the release of such enzymes from monocytes and that such release may be a factor in the production of the chronic inflammation seen in these disorders. | |
| 6204949 | Association of C1q- and conglutinin-binding immune complexes with high consumption of alph | 1984 | The consumption of alpha-2-macroglobulin (a2M) by proteinases in synovial fluids (SF) was indirectly measured by determining trypsin binding to solid-phase immunoadsorbed SF-a2M. In addition immune complexes (IC) were assessed by the solid-phase C1q-binding assay as well as the solid-phase conglutinin binding assay in rheumatoid (RA; n = 25) and osteoarthritic (OA; n = 9) SF. Elevated levels were found as follows: a2M X proteinase complexes (a2M X P) in 73.5% (RA, n = 20; OA, n = 5), solid-phase C1q-binding IC (C1q-IC) in 52% (13 out of 25 RA-SF) and bovine conglutinin binding IC (KgB-IC) in 54% (13 out of 24 RA-SF). All OA-SF were in either IC assay negative. Consumption of a2M in OA-SF and seronegative RA-SF was not more than 15% of total SF-a2M, showing a mean value of 19.5 and 13.5 micrograms a2M X P/ml SF. Seropositive RA-SF had an a2M X P mean value of 96.4 micrograms/ml SF, which differed significantly (p less than or equal to 0.05) from the former two groups. Similarly, RA-SF which were negative in either IC assay had a low a2M X P mean value (20.0 micrograms/ml SF). This contrasted sharply with the a2M X P means obtained in IC-positive RA-SF, which amounted to 131.5 micrograms a2M X P/ml SF in C1q-IC positive RA-SF and to 110.4 micrograms a2M X P/ml SF in KgB-IC positive RA-SF, respectively.(ABSTRACT TRUNCATED AT 250 WORDS) | |
| 576689 | Circulating autoantibody against human myoglobin in polymyositis. | 1977 Apr 25 | A passive hemagglutination method for circulating autoantibody to purified human skeletal muscle myoglobin has been developed. This antibody was detected in the sera of 22 of 31 patients with polymyositis. The incidence and the antibody titers were significantly higher than in other myopathies such as myasthenia gravis (P less than .02), Duchenne-type muscular dystrophy (P less than .001), and other conditions (P less than .001). This new antibody test is useful in diagnosing polymyositis. | |
| 6967254 | Characteristics of antibody to denatured DNA in sera of patients with systemic lupus eryth | 1980 Jun | Antibody to native (n-)DNA and denatured (d-)DNA were detected simultaneously and quantitatively in patients who had systemic lupus erythematosus (SLE) and other rheumatic diseases by the Millipore Filter method. In a group of patients who had SLE, 94% had antibody to both n-DNA and d-DNA; 6% had antibody to d-DNA only; serum that had antibody to n-DNA was not found. On the other hand, some of the patients who had progressive systemic sclerosis, dermatomyositis, polymyositis, and Sjøgren's syndrome had antibody to d-DNA only. In order to estimate the participation of anti-d-DNA antibody to lupus nephritis, patients who had SLE were classified into two groups according to immunofluorescent glomerular stainings. In a group of patients whose sera had lumpy or granular stainings, the sera reacted predominantly with n-DNA. In contrast, in the other group of patients whose sera had mesangial or linear glomerular stainings, the sera had antibodies reactive with d-DNA predominantly. These difference of reactivity between the two groups were statistically significant (P less than 0.02). The results suggest that the antibody to d-DNA is less relevant to the severity of lupus nephritis. in the evaluation of disease activity and prognosis of patients who have SLE, it will be of value to estimate the nature of anti-DNA antibodies. | |
| 6341205 | Autoantibodies to nonhistone nuclear antigens and their clinical significance. | 1983 May | Identification of the immunologic specificity of antinuclear antibodies (ANAs) in the various systemic rheumatic diseases has become increasingly important. The standard immunofluorescence technique may enable detection of antibodies to nuclear antigens present in abundance in the nucleus, such as DNA, histones, Sm, nuclear ribonucleoprotein (nRNP), and SS-B/La. The nuclear antigens present in low concentrations, such as SS-A, proliferating cell nuclear antigen (PCNA), rheumatoid arthritis nuclear antigen (RANA), and Ku antigens, are unique to cell types, and their detection requires special substrates or reagent systems. Anti-Sm, anti-Scl-70, anticentromere, and anti-PM-1 are characteristic serologic markers for systemic lupus erythematosus, scleroderma, the CREST syndrome of scleroderma, and polymyositis, respectively. Distinct profiles of ANA characterize different rheumatic diseases. A number of ANAs are found in SLE, whereas other diseases are characterized by the presence or absence of a certain ANA or by differences in mean ANA titers. Specific ANAs have been used to isolate and characterize nuclear antigens at molecular and functional levels. | |
| 6608834 | [Iron and iron binding proteins in inflammations and tumors]. | 1984 Feb 29 | Iron and iron binding proteins are involved in various regulatory mechanisms in infections and tumors. Chronic infections and tumors are the most common reasons of anemias in hospitalized patients in industrial countries . Although a lot of investigative work has been done to identify the underlying mechanisms many details are still poorly understood. This paper gives a review of our present knowledge from experimental work, reported in the literature together with own results. The main emphasis is laid on new findings about the importance of a possible regulatory role of iron and iron bindings proteins on the surface of cells of the immune system in the immunosurveillance as well as in non immune functions. Using a cytotoxic assay with a monoclonal antiplacental ferritin antibody which was developed by C. Moroz, surface ferritin was not only detected on peripheral mononuclear cells of patients with early stages of breast cancer but also in patients with rheumatoid arthritis which has not been reported so far. Possible connections of inflammatory states with tumors are discussed regarding the results with ferritin bearing lymphocytes together with other findings about the role of cell bound iron binding proteins in conditions of tumor and inflammation. | |
| 4017285 | The splenic extraction ratio of antibody-coated erythrocytes and its response to plasma ex | 1985 Jun | Splenic blood flow was measured in a series of normal subjects and patients with connective tissue diseases by measuring the rate of equilibration and the partition of 111In-labelled autologous platelets between blood and spleen. These data were used to quantify the role of splenic blood flow in determining the splenic clearance of IgG coated erythrocytes (IgG-RBC) from the circulation. Previous studies have interpreted the clearance rates of IgG-RBC only in terms of splenic reticuloendothelial function. Splenic blood flow was increased in seven of eight patients with systemic lupus erythematosus (SLE), six of 10 patients with rheumatoid arthritis (RA) and in all five patients with essential mixed cryoglobulinaemia (EMC) compared with a series of thirteen normal subjects. Expressing the rate constant of clearance of IgG-RBC as a fraction of splenic blood flow gave a value for the 'extraction ratio' of IgG-RBC (a specific measurement of reticuloendothelial function, corrected for splenic blood flow). Normal splenic extraction ratio of IgG-RBC was calculated to be 32%. All the patients with SLE and with EMC had reduced extraction ratios (in seven out of 13 patients less than 10%). In RA the extraction ratio tended to be normal (average 27.3%) but variable (9-59%). Following plasma exchange in nine patients, a significant increase in IgG-RBC extraction ratio (average of 39% with respect to pre-exchange values, P less than 0.05) was found. In contrast there was no significant change in extraction ratio following pulse methylprednisolone therapy in a further nine patients. Although the rate constant of clearance of IgG-RBC decreased by an average of 33% (P less than 0.01) in the latter group, it was matched by an equal decrease of splenic blood flow (average 37%, P less than 0.01) and so extraction ratio showed no change. These data indicate that quantification of splenic reticuloendothelial function requires measurement of both IgG-RBC clearance and of splenic blood flow. | |
| 3888490 | Clinical pharmacokinetics of the salicylates. | 1985 Mar | The use of salicylates in rheumatic diseases has been established for over 100 years. The more recent recognition of their modification of platelet and endothelial cell function has lead to their use in other areas of medicine. Aspirin (acetylsalicylic acid) is still the most commonly used salicylate. After oral administration as an aqueous solution aspirin is rapidly absorbed at the low pH of the stomach millieu. Less rapid absorption is observed with other formulations due to the rate limiting step of tablet disintegration - this latter factor being maximal in alkaline pH. The rate of aspirin absorption is dependent not only on the formulation but also on the rate of gastric emptying. Aspirin absorption follows first-order kinetics with an absorption half-life ranging from 5 to 16 minutes. Hydrolysis of aspirin to salicylic acid by nonspecific esterases occurs in the liver and, to a lesser extent, the stomach so that only 68% of the dose reaches the systemic circulation as aspirin. Both aspirin and salicylic acid are bound to serum albumin (aspirin being capable of irreversibly acetylating many proteins), and both are distributed in the synovial cavity, central nervous system, and saliva. The serum half-life of aspirin is approximately 20 minutes. The fall in aspirin concentration is associated with a rapid rise in salicylic acid concentration. Salicylic acid is renally excreted in part unchanged and the rate of elimination is influenced by urinary pH, the presence of organic acids, and the urinary flow rate. Metabolism of salicylic acid occurs through glucuronide formation (to produce salicyluric acid), and salicyl phenolic glucoronide), conjugation with glycine (to produce salicyluric acid), and oxidation to gentisic acid. The rate of formation of salicyl phenolic glucuronide and salicyluric acid are easily saturated at low salicylic acid concentrations and their formation is described by Michaelis-Menten kinetics. The other metabolic products follow first-order kinetics. The serum half-life of salicylic acid is dose-dependent; thus, the larger the dose employed, the longer it will take to reach steady-state. There is also evidence that enzyme induction of salicyluric acid formation occurs. No significant differences exist between the pharmacokinetics of the salicylates in the elderly or in children when compared with young adults. Apart from differences in free versus albumin-bound salicylate in various disease states and physiological conditions associated with low serum albumin, pharmacokinetic parameters in patients with rheumatoid arthritis, osteoarthritis, chronic renal failure or liver disease are essentially the same.(ABSTRACT TRUNCATED AT 400 WORDS) | |
| 6307569 | Studies on the relationship between HLA DR4 and in vitro IgM rheumatoid factor production. | 1983 Apr | The relationship between the presence of the DR4 antigen and other HLA antigens and in vitro production of IgM-rheumatoid factor by lymphocytes from a group of healthy young subjects was examined. Pokeweed and Epstein--Barr virus-stimulated lymphocyte cultures were examined for the production of rheumatoid factor and immunoglobulins. No significant correlation was found between the presence of the DR4 antigen and in vitro production of IgM rheumatoid factor. The presence of the B18 antigen seemed to identify a population of nonresponders when stimulated with PWM. |