Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 239231 | Mixed IgM-IgG cryoglobulinemia terminating in polyarteritis nodosa. | 1975 Jun | A case of mixed IgM-IgG cryoglobulinemia and polyarteritis nodosa is described. The patient exhibited non-deforming arthritis, Raynaud's phenomena, cutaneous vasculitis, and a sensory neuropathy.. The terminal phase of the illness was characterized by hypertension and retroperitoneal hemorrhage, with widespread large vessel arteritis. The isolated cryoprecipitate contained IgM, IgG, and C3 and possessed rheumatoid factor activity. Mild reduction in serum complement (CH50 and C3) was noted on one occasion. The evidence that these cryoprecipitable proteins may be acting as immune complexes and contributing to the arterial lesions is reviewed. | |
| 966816 | Intrasynovial injection of steroids uses and abuses. | 1976 Oct | Intra-articular injection of steroids is a valuable method of treatment, but selection of the appropriate clinical setting is required to obtain maximal benefits and to minimize the complications. All intra-articular injections must be performed under strict aseptic technique. The rheumatoid patient with one or two joints that resist systemic therapy and the patient with an occasional osteoarthritic joint with an acute inflammation are amenable to this treatment. The patient who has acute attacks of tendinitis, pseudogout, or bursitis will obtain significant benefits. Selected patients with traumatic and acute gouty arthritis also may benefit. Repetitive injections appear to be contra-indicated as they may create an environment conducive to joint destruction. | |
| 300090 | Characterization of DNA used to assay sera for anti-DNA antibodies; determination of the s | 1977 Feb | Commercial 14C-labeled KB cell DNA, widely used to assay sera for anti-DNA antibodies, was chromatographed on benzoylated-naphthoylated-DEAE-cellulose (BNDC) and on hydroxyapatite (HAP). On BNDC, only 25% of the 14C label eluted with 1 M NaC1 (KB fraction I) characteristic of ds-DNA. Fifty-five percent of the label eluted with 50% formamide-1 M NaC1 (KB fraction II) characteristic of ss or denatured DNA. On HAP, however, none of the 14C label eluted with 0.2 M phosphate buffer as anticipated for ss-DNA, but, rather, all of the 14C label eluted with 0.4 M phosphate, characteristic of ds-DNA. after pretreatment with S1 endonuclease of Aspergillus oryzae, which selectively digests ss regions, however, 42% of the 14C label was lost from the 0.4 M phosphate peak. These results indicated that more than half of this 14C-KB-cell DNA preparation was ds-DNA with ss regions which was undetectable by HAP chromatography. 3H-ds-DNA and circular 3H-ss-DNA prepared from T7 and phiX174 bacteriophage, respectively, were found to be chromatographically pure on both BNDC and HAP. None of 10 non-SLE sera (rheumatoid arthritis 3, mixed connective tissue disease 4, scleroderma 1, ulcerative colitis 1, and pulmonary fibrosis with chronic active hepatitis 1), previously believed to contain anti-ds-DNA antibodies on the basis of KB cell DNA testing and detectable antibodies against KB fraction 1 or T7 DNA: all of 10 KB cell DNA positive SLE sera had antibodies against both. Additionally, none of the 10 non-SLE sera had antibodies against KB cell DNA when retested with DNA that had been pretreated with S1 endonuclease. Seven of these 10, however, as well as all 10 SLE sera, had antibodies against phiX174 DNA, KB fraction II DNA and alkali-denatured T7 DNA. The data support the conclusions that 1) false positive tests for anti-ds-DNA antibodies can result from contamination of ds-DNA with ds-DNA having ss regions, and 2) non-SLE sera do not contain antibodies specific for ds-DNA at levels comparable to those found in SLE sera but rather contain high levels of antibodies reacting with ss regions or mixed DNA. | |
| 2411763 | Immunologic and structural studies of the lupus/Sjögren's syndrome autoantigen, La/SSB, w | 1985 Aug | La/SSB is a small nuclear RNA protein against which precipitating autoantibodies are made in many patients with systemic lupus erythematosus or Sjögren's syndrome. The recent purification of La/SSB has made structural and immunologic studies possible. Consequently, a mouse hybridoma antibody (La1) was raised, after immunization and fusion, that reacted with bovine La/SSB. Results of inhibition tests with tissue extracts and fluorescent antinuclear antibody tests demonstrated that La1 reacted with bovine extracts and cells, but not with those from human, mouse, or rabbit sources. La1 reacted in Western blot and in an adapted anti-La/SSB enzyme-linked immunosorbent assay with only the 41-kD bovine La/SSB peptide and not with the smaller 29-kD bovine La/SSB peptide. RNA gels showed that La1 bound the La/SSB particle that contained the predominant La/SSB RNA species near 90 nucleotides as well as the minor RNA species, both of which were bound by the human autoimmune anti-La/SSB serum. A solid-phase assay for human autoimmune anti-La/SSB antibody using La1 was more sensitive for the detection of human anti-La/SSB than was a comparable assay using purified La/SSB, and showed that anti-La/SSB is present in nearly all Ro/SSA precipitin-positive sera. Thus, this study demonstrates that monoclonal antibody can be raised against La/SSB; that the protein moiety of bovine La/SSB differs from human, mouse, and rabbit at an epitope on the 41-kD La/SSB peptide; that the RNA bound to the La1-reactive particle was as heterogeneous as that binding the anti-La/SSB autoimmune serum; and that anti-Ro/SSA and anti-La/SSB are closely associated. | |
| 4022842 | Pulmonary polymorphic centroblastic type malignant lymphoma in a patient with lymphomatoid | 1985 May | A case of lymphomatoid granulomatosis arising in a patient with a long history of dysimmunity is reported. The autopsy reviewed the final evolution into a high grade malignant lymphoma with IgM K immunoglobulin production and discovered in deep lymph nodes modifications similar to angio-immunoblastic lymphadenopathy. | |
| 7066781 | Oral and labial biopsies in amyloidosis and Sjögren's syndrome: meeting and pathologist's | 1982 Mar | An oral biopsy in a patient suspected of having amyloidosis should be taken from any clinically suspicious area present in the oral mucosa. Such areas include petechiae, ecchymoses, macules, papules, plaques and nonspecific ulcers. If none of these are found, a nondirected biopsy of the gingiva and alveolar mucosa should be performed. The marginal gingiva should be avoided. The labial biopsy for Sjögren's syndrome involves making an incision 1.5 to 2 cm long, parallel to the vermilion border, in the labial mucosa of the lower lip. The incision is widened by blunt dissection and four to seven nodules of salivary gland need to be removed for examination. | |
| 6474196 | Labeled cells in the investigation of hematologic disorders. | 1984 Jul | Radiolabeling techniques for white cells, platelets, and erythrocytes are reviewed. The early studies using diisopropylfluoro-32P contributed to an understanding of the production and circulation of the blood elements, and 51Cr proved useful in localizing sites of cell migration or destruction. 111In-oxine has further improved the understanding of blood cell organ sequestration, and permitted combined kinetic and organ imaging studies. Radionuclide labels have been essential for the elucidation of various hematologic disorders, such as the neutropenias, thrombocytopenias, anemias, and polycythemia. Many new treatments, including monoclonal antibodies, have been evaluated with radionuclides. | |
| 6715882 | Partial immunochemical characterization of the Ro and La proteins using antibodies from pa | 1984 May | Ro and La are intracellular ribonucleoproteins that are frequent targets for autoantibodies in the sicca syndrome and in lupus erythematosus. We analyzed the m.w. of the protein (antigen) moieties of Ro and La in saline cell extracts of human spleen by SDS-PAGE and immunoblotting, gel filtration, and sucrose density ultracentrifugation with radioimmunoassay. pI values for Ro and La proteins were established by isoelectric focusing on thin layer agarose gels and immunoblotting on nitrocellulose transfers. The La protein had an m.w. of approximately 43 kd and was heterogeneous in charge, with pI values from 4.2 to 4.8. Composite two-dimensional maps developed by immunoblotting revealed a characteristic set of seven dots of m.w. 43 kd. Ro determinants were identified on polypeptides of 50 and/or 57 kd. Antigenic activity was also detected in the void volume of spleen extract fractionated by Sephadex G-200 and in 8 to 9S and greater than 19S regions of sucrose gradients, suggesting either aggregation of the Ro protein or participation in protein-protein complexes. pI values of 4.3 to 5.5 were obtained for the Ro antigen, and two-dimensional maps revealed that the 57 kd polypeptide had a similar charge heterogeneity to the La protein, whereas the 50 kd polypeptide had a different fingerprint. Immunoblotting of extracts from bovine, rabbit, and dog extracts showed that antibodies to Ro and La reacted with a limited number of polypeptides (m.w. 50 and/or 57 kd for anti-Ro and 43 or 50 kd for anti-La). These studies support the physical independence of the isolated Ro and La polypeptides, although a precursor product or functional relationship in vivo is possible. These studies also suggest that, in addition to Western blotting, techniques involving immunodeletion, isoelectric focusing with capillary immunoblotting, and 2D immunoblotting provide useful approaches to characterize saline-soluble cellular antigens. | |
| 6731383 | Neonatal lupus erythematosus. | 1984 Jul | Three infants with neonatal lupus erythematosus (LE) had characteristic sharply marginated erythematous papules and plaques without congenital heart block. Two of the infants were definitely photosensitive, while the third infant's eruption began on a photoexposed area. All three infants and their mothers were positive for Ro Sjögren's syndrome A antigen (SSA) antibody. These three infants have additional evidence supporting the concept that transplacental passage of Ro (SSA) antibody is a serologic marker for neonatal LE. | |
| 6358997 | Effect of Sialor in treatment of xerostomia in Sjögren's syndrome. | 1983 Nov | Sialor, a sialogogue, was studied in a double-blind crossover trial in patients with autoimmune exocrinopathy (Sjögren's syndrome). Sialor appears to frequently alleviate the symptoms of xerostomia and increase salivary flow rate, and it is rarely associated with side effects. Sialor is useful in management of xerostomia in patients with Sjögren's syndrome and may also be of value in the treatment of xerophthalmia. | |
| 6345604 | Differential staining pattern of lysozyme in palisading granulomas: an immunoperoxidase st | 1983 May | Biopsies from palisading granulomas of granuloma annulare, necrobiosis lipoidica, and rheumatoid nodules were examined for the presence of lysozyme (muramidase). This enzyme was identified in paraffin-embedded tissues using a primary antibody to lysozyme and the peroxidase-antiperoxidase technic. Some inflammatory cells in the infiltrate of granuloma annulare stained abundantly for lysozyme, whereas those of necrobiosis lipoidica and rheumatoid nodule stained minimally and negatively, respectively. This pattern of staining may be of diagnostic value and suggests that the histiocytoid cells constituting the infiltrate of granuloma annulare are in some way different from the similar-appearing cells of necrobiosis lipoidica and rheumatoid nodule. | |
| 7041242 | Effect of Saliment on parotid salivary gland secretion and on xerostomia caused by Sjögre | 1982 Apr | The stimulatory effect of a recently introduced saliva substitute (Saliment) on the parotid salivary secretion was experimentally investigated in 10 healthy individuals without signs or symptoms of xerostomia and in 11 patients with xerostomia due to Sjögren's syndrome. Further, in 15 patients with Sjögren's syndrome, the effect of Saliment on the sensation of xerostomia was examined in a double-blind clinical study with cross-over. The use of Saliment was followed by a significant stimulation in the parotid salivary secretion (P less than 0.01) and the subjection sensation of xerostomia was significantly reduced (P less than 0.01). | |
| 7027431 | Bromhexine treatment of Sjögren's syndrome: effect on lacrimal and salivary secretion, an | 1981 | In a randomized clinical study, 32 patients with Sögren's syndrome (SS were given placebo/bromhexine (Bisolvon) 48 mg daily for 3 weeks. Various ophthalmological and oral variables as well as different proteins in the tear fluid and saliva were registered. Bromhexine stimulated the tear secretion significantly, presumable without changing the protein composition of the tear fluid. In contrast to other proteins in saliva the IgM concentration decreased in patients with high pre-treatment values. Bromhexine may be of valuable help in the treatment of some patients with SS. | |
| 335548 | Tear physiology and dry eyes. | 1977 Sep | The conditions of tear film formation and stability are governed by the surface chemical characteristics of the tear film system and by the proper functioning of the lacrimal apparatus. The tear film has to remain continuous between blinks in order to fulfill it function. The presence of an abnormal tear film results in dry eye states that can be detrimental to vision. The diagnostic tests presently available are limited mainly to approximately determining tear secretion rate and estimating epithelial damage by staining techniques. The only test that directly measures tear film stability is one which determines tear film breakup time. The treatment modalities depend on the type of irregularity causing the dry eye state and range from the application of artificial tear substitutes or the obstruction of the puncta to surgical alterations of the lacrimal system. | |
| 937245 | Changes in serum salivary isoamylases in Sjögren's Syndrome. | 1976 Jun | Quantitative polyacrylamide disk gel electrophoresis of sera from 27 patients with Sjögren's syndrome (SS) and 12 comparable normal subjects revealed that serum amylase activity in patients with SS varies due to changes in the salivary isoenzyme, while pancreatic isoamylase remains normal. The SS group can be divided into those patients with markedly increased salivary isoamylase and those with normal or low salivary isoamylase. At this time we cannot be certain whether this reflects different stages in a progressive disorder, or differences in the underlying pathologic processes. Analysis of amylase isoenzymes in serum previously has been shown to be of value in pancreatic disorders, and we have now demonstrated that changes in the salivary glands may also be relected in serum amylase isoenzymes. Study of patients with other salivary and pancreatic disorders will be needed to define the clinical utility of amylase isoenzyme analysis. | |
| 3876599 | Development of chronic lymphocytic leukaemia in a case of Sjögren's syndrome with systemi | 1985 Aug | Persistent lymphocytosis and intermittent fever were found in a 68-yr-old Chinese woman 5 yr after the diagnosis of Sjögren's syndrome with systemic lupus erythematosus (SLE). A series of examinations--including virology, bone marrow aspiration and surface markers of lymphocytes--was made to evaluate the nature of the lymphocytosis which had not been found previously. All of the results were consistent with the diagnosis of B-cell chronic lymphocytic leukaemia (CLL). Development of CLL in Sjögren's syndrome has seldom been described before and may be added to other malignancies associated with Sjögren's syndrome. | |
| 3988886 | Monoclonal gammopathies in Japanese patients with Sjögren's syndrome. | 1985 Mar | We report 10 Japanese patients with Sjögren's syndrome (SS) who developed monoclonal gammopathies (MG). One was of the IgG class, five of IgA, three of IgM, and one of IgG/IgM. The monoclonality of 7 of 10 M proteins was studied using antiidiotypic (Id) antibodies against M proteins. Four (three IgA and one IgM) of 10 M proteins had rheumatoid factor (RF) activity. Hemagglutination inhibition tests and enzyme-linked immunosorbent assays (ELISA) showed that the RF activity was inhibited by anti-Id antibodies in all four monoclonal RFs. In two patients examined, many cells infiltrating into the salivary glands were stained with anti-Id antibodies. Our review of 19 Japanese SS patients with MG revealed that the non-IgM class predominated (13/19). This contrasts with 19 reported non-Japanese SS patients, among whom 14 were IgM. In both Japanese and non-Japanese patients there was a higher incidence of MG in primary than in secondary SS. The difference in the dominant heavy-chain class may reflect a difference in the genetic factors affecting B cell differentiation in immunologically disordered states. | |
| 6525062 | [Clinico-morphological characteristics of Sjögren's syndrome (biopsy and autopsy data)]. | 1984 | Sixty patients with Sjogren's disease (SD) were examined. In all the patients--women aged 23 to 70, apart from the generalized "dry syndrome", there were signs of involvement of other organs and systems. Biopsy and autopsy material was studied histologically and histochemically, in some cases immunofluorescent and electron-microscopical study was performed. The most pronounced morphological and clinical manifestations of SD were linked with the damage to exocrine glands. Their alterations were represented by delayed type hypersensitivity and were similar in all exocrine glands, hence the exocrine tissue might be regarded as a target tissue in SD. One of the mechanisms of the exocrine tissue damage is connected with a mutual duct antigen of exocrine glands represented by the serum component IgA. The involvement of certain organs and systems with the development of chronic persistent hepatitis, fibrosing alveolitis, mesangioproliferative and tubulointerstitial nephritis, pronounced hyperplasia of lymphoid tissue with its plasmatization and generalized affection of the microcirculatory system of the immunocomplex nature, is noted in SD with various frequency. | |
| 6395135 | A review of clinical trials of lithium in medicine. | 1984 | Since the approval of lithium use in treatment of acute mania, there have been numerous clinical trials of lithium in medical and psychiatric disorders. This paper gives a brief review of the literature on lithium trials in approximately fourteen medical conditions. These are: hyperthyroidism, metabolizing thyroid cancer, syndrome of inappropriate secretion of antidiuretic hormone, premenstrual tension syndrome, anorexia nervosa, Felty's syndrome, chemotherapy-induced neutropenia, aplastic anemia, seborrheic dermatitis, eczematoid dermatitis, cyclic vomiting, diabetes mellitus and asthma. Most of the case reports cited showed the efficacy of the side effects from lithium salt in the management of the symptoms and signs of these disorders, however, well-designed and controlled studies give negative results. The positive results are reported in the group of disorders having an underlying subdromal affective syndrome such as premenstrual tension syndrome and anorexia nervosa. Other encouraging reports include the effect of lithium to induce leucocytosis in Felty's syndrome and chemotherapy-induced neutropenia. | |
| 6185917 | [Salivary lysozyme, immunoglobulins and beta 2-microglobulin in Gougerot-Sjögren syndrome | 1982 Nov 20 | Lysozyme (LZM), immunoglobulins and beta 2-microglobulin concentrations were measured in the saliva of 79 patients with primary (n = 29) or secondary (n = 50) Sjögren's syndrome and in a control population. beta 2-microglobulin and immunoglobulins A, G and M concentrations were higher, and LZM concentrations lower than in controls. A significant correlation was established between LZM and immunoglobulins, but no correlation was found between immunoglobulins and beta 2-microglobulin, nor between the latter and LZM. These results indicate that salivary LZM concentration may be of value in the diagnosis of Sjögren's syndrome. |