Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 3954468 | Differential effects of therapeutic regimens on specific classes of rheumatoid factor. | 1986 Mar | This study was performed to define further the relationship of circulating IgM and IgG rheumatoid factor to the articular manifestations of rheumatoid arthritis. Patients with substantial clinical improvement (greater than 55%) or no improvement or worsening were chosen for study. Patients were further selected to include those treated with non-steroidal anti-inflammatory drugs (NSAIDs) alone, or NSAIDs plus D-penicillamine or gold. Significant reductions of IgM (p less than 0.001) and IgG (p less than 0.005) rheumatoid factor were seen only in those treated with gold who improved clinically. These observations suggest that in patients experiencing comparable degrees of clinical improvement the simultaneous alterations of circulating IgG and IgM rheumatoid factor observed are dependent upon the therapeutic regimen employed. | |
| 1747701 | Single cell imaging reveals abnormal intracellular calcium signals within rheumatoid synov | 1991 Dec | Intracellular calcium (Ca2+) signalling in synovial fluid (SF) polymorphonuclear leucocytes (PMN) from patients with rheumatoid arthritis (RA) was compared to RA and normal circulating blood PMN using single cell imaging. RA SF PMN stimulated by the peptide f-Met-Leu-Phe (FMLP) showed a striking difference in the release of Ca2+ from the intracellular store compared to RA and normal circulating blood PMN. Stimulation caused the release of a very dispersed, nonrestricted 'cloud' of Ca2+ in 60% of RA SF PMN compared to the highly localized and restricted 'cloud' observed in only 30% of normal circulating PMN. In the presence of extracellular Ca2+, both RA SF and normal blood PMN showed heterogeneity in both the timing and magnitude of their cytosolic free Ca2+ signalling. These observations imply that the Ca2+ signalling mechanism in RA SF and RA blood PMN has been primed in a way which could exacerbate the release of inflammatory mediators. This may have serious implications for explaining the aberrant behaviour of SF PMN in RA. | |
| 2591127 | NAD synthesis and ADPR transferase activity in blood lymphocytes of patients suffering fro | 1989 Sep | NAD acts not only as a coenzyme in oxidation-reduction processes but also as substrate for adenoribosylation of different proteins. Patients suffering from rheumatoid arthritis show a disturbance in their tryptophan metabolism. Since tryptophan represents a precursor of NAD we turned our attention to the activity of the ADPR transferase, being the enzyme of adenoribosylation, as well as to the synthesis of NAD derived from nicotinamide in human lymphocytes. Lymphocytes of patients with rheumatoid arthritis did not show any differences in their activities as compared with those of control persons, regardless of different measures applied to the patients. | |
| 2898244 | A controlled trial comparing sulfasalazine, gold sodium thiomalate, and placebo in rheumat | 1988 Jun | One hundred eight-six patients with active rheumatoid arthritis were evaluated in a double-blind, randomized study that compared treatment with sulfasalazine (SSZ) (2 mg/day), gold sodium thiomalate (GST) (50 mg/week), and placebo (PBO). The 37-week course of therapy was completed by 109 patients. While marked improvement was seen in all 3 treatment groups, the only statistically significant differences between SSZ or GST and PBO were in a decreased erythrocyte sedimentation rate and increased grip strength in the right hand. GST is known to be superior to PBO, and the response of the GST-treated group was similar to that seen in other trials. The response of the PBO group, however, was much greater than in other placebo groups we have studied. SSZ was similar in efficacy to injectable gold, but was better tolerated. Because of adverse drug reactions (most commonly, rash, stomatitis, and proteinuria), 41% of patients were withdrawn from the GST treatment. Untoward drug effects (most frequently, rash and gastrointestinal distress) caused 16% of patients to be withdrawn from SSZ therapy. | |
| 2289324 | Zinc profiles in rheumatoid arthritis. | 1990 Nov | Evidence has accumulated which suggests that zinc may play an important role in immune function and inflammation. In this study we examined the zinc status in patients with rheumatoid arthritis (RA), osteoarthritis, other rheumatic diseases and healthy controls. Results indicate that serum and plasma zinc levels are decreased significantly in patients with RA. Reduced zinc values may be due to decreased albumin-bound and micromolecular protein-bound zinc. | |
| 3133152 | Histocompatibility antigens in adult obliterative bronchiolitis with or without rheumatoid | 1986 Jun | We compared HLA antigens in 28 patients with obliterative bronchiolitis (16 with rheumatoid arthritis) with those in 150 normal controls, to determine their relationship to drug toxicity and to the pathogenesis of obliterative bronchiolitis (OB). There was a significantly increased prevalence of HLA-B40 in the whole group and in patients with OB and rheumatoid arthritis (RA). There was increased frequency of HLA-A2 and A28 within the whole group and in those with OB alone. HLA-A3 and B5 were completely absent in our patients. Of those antigens most frequently associated with RA, DR4 was significantly more prevalent in patients with OB, with or without RA, but DR1 occurred more frequently only in those with RA-associated OB. Increased prevalence of DR4 may indicate an association between the pathogenesis of both OB and RA and the HLA-DR locus. Although D-penicillamine has been implicated in the pathogenesis of OB our patients did not show an increase in those antigens associated with other gold/penicillamine toxicity (i.e. DR2/3), suggesting that they may constitute a distinct group. | |
| 2036201 | Effect of joint mobilization on serum hyaluronate. | 1991 Feb | Serum hyaluronate was monitored in volunteer patients with rheumatoid arthritis who were mobilized on day 1 at 7 am, but stayed at rest in bed on day 2 until mobilized at 11:30 am. 125I-hyaluronate binding protein assay disclosed a normal morning peak on day 1 but not on day 2. Instead, on day 2 transiently high hyaluronate levels were seen after 11:30 am. This suggests that joint mobilization affects serum hyaluronate. The morning peak may depend on the accumulation of hyaluronate in immobilized joints at night and its mobilization in the morning. High performance liquid chromatography with a size exclusion column used for fraction collection with 125I-hyaluronate binding protein assay showed that the serum hyaluronate in the morning peak is of relatively low molecular weight when compared to synovial fluid hyaluronate. This difference may be due to degradation of synovial fluid hyaluronate before its clearance into the blood stream. | |
| 2344207 | Precision of Larsen grading of radiographs in assessing progression of rheumatoid arthriti | 1990 May | A study was designed to evaluate observer variation in the assessment of radiographic deterioration of individual patients using the Larsen grading system. Radiographs of hands and feet of 52 patients were assessed by three observers. Each patient had paired films taken one year apart which were assessed together for change in score. To assess within-observer variation each set of films was read twice by all observers. The average progression was 11.6 (SD 9.0). Analysis of the source of variation showed the single observer replication SD to be 3.7 but that for different observers to be 5.5. This may be interpreted as indicating that to achieve 95% confidence of detecting a true change an increase in Larsen score of 8 is required if the same observer assesses or up to 11 if a different observer assesses. | |
| 2756690 | [Indices of the blood fibrinolytic system in osteoarthrosis and rheumatoid arthritis]. | 1989 Apr | The fibrinolytic system of the blood was studied in 46 patients with osteoarthrosis (OA) associated with reactive synovitis or without it, 44 patients with rheumatoid arthritis (RA) without visceral manifestations. It was found that a slowing of enzymatic, nonenzymatic and XIIa-dependent fibrinolysis is typical of RA but not of OA. Products of fibrin breaking in the general blood flow were absent in patients with OA but may be observed in RA. Increase of the level of fibrin monomers (FM) in the blood are observed in OA with reactive synovitis. The concentration of FM in the blood in RA and OA with reactive synovitis is an informative and dynamic sign of the activity of the inflammatory process and efficacy of treatment. | |
| 3813675 | Drug induced impairment of polymorphonuclear cell bactericidal ability in rheumatoid arthr | 1987 Jan | Thirty three patients with rheumatoid arthritis (RA) treated only with non-steroidal anti-inflammatory drugs were divided into two groups according to the drug received (group A: diclofenac, group B: indomethacin or ketoprofen), and their polymorphonuclear (PMN) cell functions were investigated. We found that bactericidal ability was the only function significantly impaired in group A as compared with group B patients and normal controls. This modification correlated well with the reduction of control PMN bactericidal ability when this test was carried out in patient plasma. These differences between drug effects might explain some of the discrepancies between reports of PMN cell functions in RA. | |
| 3518772 | Quantitation of dose and concentration-effect relationships for fenclofenac in rheumatoid | 1986 Apr | Response to non-steroidal anti-inflammatory drugs (NSAIDs) is not usually assessed on the basis of concentration measurements: identification of a concentration-effect relationship has proved difficult to achieve. Dose and concentration-effect relationships of fenclofenac have been determined in a group of 18 patients with rheumatoid arthritis at three dose levels (600, 1200 and 1800 mg day-1). The study was double-blind and treatments were randomised according to a Latin square design. A multiple linear regression technique (GLIM) was used in the analysis. The best model to describe the change in effect in terms of dose and concentration incorporated an average slope and an individual subject intercept for each effect measurement. On average, an improvement in grip strength of 20 mm Hg could be obtained with an increase in fenclofenac (trough) concentration of 100 micrograms ml-1. | |
| 3122673 | Patterns of urinary excretion of gold in patients with rheumatoid arthritis undergoing chr | 1987 Nov | Thirty patients receiving gold therapy for rheumatoid arthritis (RA) were studied for the urinary excretion of gold. Statistical analysis of all the urine specimens passed over a period of four days by each patient showed that a definite rhythm of gold excretion exists for each patient which is possibly related to water excretion but not to creatinine excretion. The study indicates possible reasons for the inability of earlier workers to relate gold excretion to the general body gold status of patients and suggests that as the study of 24 hour excretions of gold may be an insensitive marker of gold excretion, closer examination of individual patient rhythms of gold excretion could possibly provide a more useful method of analysis. | |
| 2001604 | Autoantibodies in D-penicillamine-induced myasthenia gravis: a comparison with idiopathic | 1991 Mar | The distribution of autoantibodies was studied in patients with rheumatoid arthritis (RA) treated by D-penicillamine and who developed myasthenia gravis (MG). The anti-human acetylcholine receptor (AChR) antibodies were specifically associated with clinical symptoms of MG without any difference in the pattern of specificities in idiopathic (id-MG) or in induced MG (DPen-MG). Conversely, anti-nuclear antibodies were elevated in DPen-MG sera compared to id-MG sera (P less than 0.001) but were also compared to patients with RA treated by D-penicillamine (or thiopronine) and who did not develop MG. Anti-denatured DNA antibodies were enhanced in sera from treated patients, whether they had presented or not a MG disease. Anti-histone antibodies were associated with RA. These observations suggest that the immunological imbalance in RA patients, can be increased by a drug treatment which may trigger the appearance of a second autoimmune disease such as MG, where anti-AChR antibodies are associated with anti-nuclear antibodies. | |
| 2327135 | [Incidence of goiter and thyroiditis in chronic inflammatory rheumatism]. | 1990 Jan 15 | In our investigations 201 patients with rheumatoid arthritis show in 127 cases according 63.2% (123 women = 71.1%; 4 women = 14.3%) and 32 patients with collagen diseases in 11 cases according 34.4% (11 women = 43.3%) a goiter. In comparison to a control group with a goiter incidence of 29.5% in women (168 out of 570 women), we found in female patients with rheumatoid arthritis or collagen diseases a clearly higher prevalence of goiter. But there was no difference of goiter frequency between the small groups of men. A second aim of our study was to investigate, whether an increased occurrence of the hypertrophic kind of Hashimoto's thyroiditis is to find in patients with rheumatic diseases, because of a possible association of two immunological induced affections. The results of sonographical, immunological and cytological examinations of the thyroid gland suggested no high responsibility of Hashimoto's thyroiditis for the goiter frequency in rheumatism. Only in three out of 201 investigated patients with rheumatoid arthritis (1.5%) we were able to diagnose this disease. In comparison to a rheumatologically healthy population out of the same catchment area (HT-frequency less than 0.5%), indeed the morbidity rate in rheumatic patients seems to be 3 times higher. But for a goiter frequency of altogether 63.2% in this disease the Hashimoto's thyroiditis is only of very small importance. Other causes such as pharmacotherapy with goitrogenic antirheumatic drugs, conditions of iodine deficiency, disposition and probably also an increase of thyroid growth stimulating immunoglobulins may be alone or in combination or a much higher degree responsible for goiter development in patients with rheumatoid arthritis or collagen diseases. | |
| 1765991 | Induction of systemic lupus erythematosus by interferon-gamma in a patient with rheumatoid | 1991 Oct | The development of systemic lupus erythematosus (SLE) after 38 months of therapy with recombinant human interferon gamma (rIFN-gamma) was observed in a patient with rheumatoid arthritis. In addition to glomerulonephritis and a butterfly rash, previously negative tests for antinuclear, anti-dsDNA and anti-Sm antibodies became positive. We assume that rIFN-gamma induced the de novo development of SLE in our patient. | |
| 2298520 | Electrophysiologic studies of the heart in patients with rheumatoid arthritis. | 1990 Jan | We investigated the electrophysiological properties of the heart in patients with definite or classical rheumatoid arthritis using programmed electrical stimulation techniques. Twelve patients with rheumatoid arthritis and without evidence of organic heart disease or arrhythmia detectable with serial electrocardiograms and 24-hour ambulatory electrocardiographic monitoring were compared with 12 control subjects. Stimulation was performed from the high right atrium and right ventricular apex at a drive cycle length of 600 msec and the recording sites included high right atrium, atrioventricular junction and distal coronary sinus. There was no statistically significant difference in the corrected sinus node recovery time between the study and control group of patients. Similarly, no differences from normal were found in the AH and HV intervals or in the atrial and ventricular refractoriness, whereas the atrioventricular nodal effective refractory period was higher in patients with rheumatoid arthritis, compared with the control group (338 +/- 38 vs 286 +/- 29, P less than 0.02). The atrial conduction time during basic cycle length had a tendency to increase from high right atrium to atrioventricular junction in the study group and reached statistical significance from high right atrium to coronary sinus (92 +/- 15 vs 74 +/- 14, P less than 0.05). Electrophysiologic differences between the study and control patients also included a greater increase in maximal intraatrial (40 +/- 13 vs 27 +/- 16, P less than 0.05) and interatrial conduction delay (54 +/- 16 vs 31 +/- 12, P less than 0.01) of early premature stimuli in patients with rheumatoid arthritis.(ABSTRACT TRUNCATED AT 250 WORDS) | |
| 3763535 | Initial evaluation of the arthritic patient. Piecing together the diagnostic clues. | 1986 Oct | Evaluation of the arthritic patient as outlined in this article should enable the physician to formulate a working diagnosis that will suggest appropriate therapy. Initial evaluation includes thorough history taking, general and rheumatologic examination, selected laboratory tests (including synovial fluid analysis where appropriate), and radiologic studies. An individualized approach to patients is necessary, since each of the rheumatic diseases can affect patients in different ways. | |
| 1696156 | Natural killer cells in the blood of patients with rheumatoid arthritis treated with azath | 1990 Aug | The mode of action of azathioprine in the treatment of rheumatoid arthritis is not known. In this study we showed that azathioprine did not produce any consistent fall of NK cell numbers as detected by the use of the monoclonal antibodies Leu 7 and Leu 11. There was a marked and significant loss of NK cell function. This was not associated with changes in disease activity following treatment. The cause for the loss of NK function is not known. | |
| 3139877 | A randomized controlled trial of parenteral methotrexate compared with sodium aurothiomala | 1988 | Forty patients with rheumatoid arthritis (RA) were enrolled in a double blind study of 26 weeks duration designed to compare the efficacy and safety of parenteral methotrexate and sodium aurothiomalate (GSTM) in the treatment of RA. All the patients had active RA and none had previously received gold, D-penicillamine or immunosuppressive therapy. Patients were randomized to receive weekly intramuscular (IM) injections of either methotrexate 10 mg or GSTM 50 mg. Two patients taking methotrexate and 7 taking GSTM were withdrawn before 26 weeks. Methotrexate was as effective as GSTM as measured by numbers of swollen or tender joints, morning stiffness, grip strength, pain scale and erythrocyte sedimentation rate. Five patients taking methotrexate and 11 taking GSTM presented side effects (p = 0.05). Total number of adverse reactions was 5 for the methotrexate group and 15 for the GSTM group (p less than 0.01). Our data suggest that low dose IM methotrexate is less toxic and as effective as GSTM for the treatment of RA during the first 6 months of therapy. | |
| 1863828 | Plasma levels of interleukin-1-alpha in rheumatoid arthritis. | 1991 Aug | Interleukin-1-beta (IL-1 beta) has been implicated as an inflammatory mediator in rheumatoid arthritis (RA) but little is known about the related cytokine, IL-1 alpha, in this disease. IL-1 alpha has biological properties similar to IL-1 beta but, unlike IL-1 beta, remains mostly cell-associated. In this study plasma IL-1 alpha was measured by radioimmunoassay in patients with RA and in healthy controls. Plasma levels were compared with conventional measures of disease activity. The mean levels in the two groups were not significantly different and, within the patient group (n = 53), the only significant cross-sectional correlation was between plasma IL-1 alpha and ESR. In longitudinal studies, some individual patients had plasma IL-1 alpha levels that correlated with different measures of disease activity. We conclude that while IL-1 alpha may be involved in the immunopathogenesis of RA, its measurement in plasma seems to offer little of clinical value. |