Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 2880382 | Phenotypic characterization of 3H-thymidine incorporating cells in rheumatoid arthritis sy | 1986 | Tritiated-thymidine incorporating cells in synovial tissue samples from ten patients with definite or classic rheumatoid arthritis (RA) were studied by combining two techniques. Tritiated-thymidine-labelled cells were seen in autoradiography and simultaneously the subtype of them was determined with immunoperoxidase staining using monoclonal antibodies. Tritiated-thymidine-labelled cells comprised 0.8 +/- 0.4% of all the inflammatory cells in RA synovial membrane. Of all 3H-thymidine-labelled cells 34 +/- 17% were positive for OKT8 and 19 +/- 8% for OKT4 monoclonal antibodies. OKM1-positive cells comprised 7 +/- 3% of all 3H-thymidine labelled cells, whereas only a few (3 +/- 4%) of them were positive for pan-B monoclonal antibody. This study emphasizes the importance of activated OKT8 lymphocytes in RA synovial membrane. | |
| 3199398 | Magnetic resonance imaging of the craniovertebral junction in rheumatoid arthritis. | 1988 Sep | Conventional radiography and magnetic resonance imaging (MRI) of the craniovertebral junction were evaluated in 12 patients with longstanding rheumatoid arthritis (RA) and neck pain with or without other neurologic signs or symptoms of cervical myelopathy. MRI demonstrated abnormal soft tissue masses thought to represent pannus in 9 patients. Three patients showed cord or brainstem compression due to pannus or atlantoaxial subluxation. The 3 patients with MRI evidence of cord or brainstem compression had neurologic signs or symptoms of cervical myelopathy, and appropriate therapy was instituted based on these findings. This study indicates that MRI is able to detect abnormal soft tissue masses which probably represent pannus and their relationship to the spinal cord or brainstem, and confirms the utility of the procedure in the management of craniovertebral involvement in RA. | |
| 3769242 | In vivo effects of chloroquine treatment on spontaneous and interferon-induced natural kil | 1986 Jul | Chloroquine is a primary amine which inhibits in vitro cell-mediated cytolysis, probably by affecting the lysosomal system. Spontaneous and interferon (INF)-induced natural killer (NK) activities were studied in patients affected by rheumatoid arthritis (RA) undergoing chloroquine therapy. In all chloroquine-treated patients spontaneous and IFN-induced NK activities were decreased, as compared with healthy controls or RA patients, not treated with chloroquine. NK activity, however, substantially increased after withdrawal of chloroquine treatment. It is suggested that chloroquine inhibits cell-mediated cytotoxicity in vivo by the same mechanisms observed in in vitro studies, and that these effects might be relevant in the therapeutic action of this drug in RA. | |
| 3498036 | Inflammatory involvement of cervical spine ligaments in patients with rheumatoid arthritis | 1987 Jun | The inflammatory involvement of cervical spine ligaments in patients with rheumatoid arthritis (RA) with atlantoaxial subluxation was studied. Biopsy specimens were obtained from the ligament structures between the posterior arch of the atlas and the spinous process of C2 during atlantoaxis stabilizing operations. Inflammatory cells in cryostat sections were stained in situ using monoclonal hybridoma antibodies applied in a sensitive avidin-biotin-peroxidase complex (ABC) method. In 4 of 8 ligament specimens, focal inflammatory cell infiltrates consisting mainly of T11 + T lymphocytes (77 +/- 9%) and M1 + monocytes (21 +/- 6%) were found. The occurrence of lymphocyte activation markers Ia, 4F2 and T9, 46 +/- 8%, 38 +/- 10% and 14 +/- 5%, respectively, suggests active involvement of the lymphocytes in the local immune inflammation. Our findings suggest a mechanism eventually leading to slackening of the cervical spine ligamentous apparatus and atlantoaxial subluxation in RA. | |
| 2362887 | Opportunistic Listeria pericardial effusion. | 1990 Mar | A case is described of pericardial effusion due to Listeria monocytogenes infection in a woman with advanced carcinoma of the cervix, rheumatoid arthritis and on corticosteroid therapy. Focal infection with listeria in immunocompromised adults has a high mortality unless promptly diagnosed. The correct diagnosis may have been made if an unexplained pericardial effusion had been tapped. | |
| 2121868 | Subclasses of IgG H chain expressed in the rheumatoid synovium. | 1990 Sep | A complementary DNA (cDNA) library was constructed from a 72 year-old patient's synovium removed at surgery of the left knee replacement. The number of independent clones was found to be 1.1 x 10(6). Eighty-seven (0.3%) positive plaques were obtained from 30,000 plaques of this library using a genomic probe containing a constant region of IgG1. In these positive plaques, ten independent clones were purified and the subclasses were identified by DNA sequences near the "Hinge" region. Seven clones were identified as gamma 2, two clones as gamma 1 and one clone as gamma 4 respectively. Measurement of the serum IgG subclasses in this patient indicated increased IgG2. We conclude that some patients of rheumatoid arthritis exhibit an increased level of a unique IgG subclass. | |
| 3729576 | Connective tissue abnormalities in MRL/1 mice. | 1986 Jun | Pathological changes in the connective tissue of the limbs of MRL/1 mice are described. Focal infiltrates of polymorphs or large mononuclear cells, or both, were seen both in synovial lining and subcutaneous tissue. Infiltrates were associated with vasculitis in some cases. Deposits of amorphous material were seen in and around joints and in foot pads. The material was more particulate and refractile than typical 'fibrinoid' and showed a positive Feulgen reaction. It was not surrounded by palisading cells and when seen in synovial tissue was not usually associated with changes in synovial lining cells. No obvious difference was seen between intra-articular and extra-articular lesions. Lesions in subcutaneous tissue occurred exclusively in the foot pads. Lymphocyte infiltration was not prominent at any site and no follicle formation was seen. Of two colonies studied, only one showed a significant increase in lining cell numbers in synovial tissue. Exercised animals had a similar distribution and severity of disease to those of matched controls. All lesions described were distinguishable from non-specific inflammatory lesions in normal control mice and MRL/++ mice on assessment of unmarked sections. The relation between these connective tissue lesions and the changes found in human chronic synovitis is discussed. | |
| 3442971 | Coexistence of cardiac lesions and thrombophlebitis in a patient with rheumatoid arthritis | 1987 Sep | A 41-year-old Japanese female developed polyarthritis with fusiform deformity. Diagnosis of rheumatoid arthritis was made and anti-inflammatory drugs were prescribed. Later thrombophlebitis in legs and cardiac lesions developed. The clinical course and treatment of this patient are briefly discussed. | |
| 3495479 | Bf and C3 polymorphisms in rheumatoid arthritis. | 1987 | Properdin factor B (Bf) and complement C3 polymorphisms were studied in 225 unrelated rheumatoid arthritis (RA) patients from North-East England. Patients were subdivided on the presence or absence of significant titres of rheumatoid factor and antinuclear factor. No association with the C3 system was detected. For the Bf system, a significant excess of Bf SS and deficiency of Bf FS phenotypes was observed in seropositive RA patients lacking antinuclear antibodies. This finding suggests that auto-antibody-defined subgroups of RA may be genetically heterogeneous with respect to Bf and confirms the status of Bf SS phenotype as a marker for RA susceptibility and/or severity. | |
| 2237673 | [Methylprednisolone-pulse therapy in a patient with rheumatoid arthritis and diffuse inter | 1990 Apr | A forty-eight year-old female with rheumatoid arthritis developed cough, sputum and dyspnea. Chest X-ray film demonstrated bilateral diffuse interstitial pneumonia and pulmonary fibrosis. Laboratory findings were as follows: ESR 29 mm/h, CRP 3.86 mg/dl, RA test (+), RAHA (-) and WBC 7200/mm3. Marked hypoxemia (po(2)45 Torr) was demonstrated by blood gas analysis. Asymptomatic pulmonary fibrotic lesions which preceded articular symptoms were identified on her previous chest X-ray films. Methylprednisolone-pulse therapy (1g/day, for 3 days) was repeated three times with a 2-week interval. The treatment rapidly improved both pulmonary symptoms and chest X-ray findings. Although the fibrotic shadows on chest X-ray did not completely disappear, her conditions have been maintained well under the treatment with prednisolone (10mg/day) plus D-penicillamine (200mg/day). | |
| 2535323 | Effects of chronic and acute corticosteroid therapy on zinc and copper status in rheumatoi | 1989 Jun | The modifications in plasma, erythrocyte and urine zinc and copper concentrations induced by chronic or acute corticotherapy were studied in patients suffering from rheumatoid arthritis and the results compared with those from controls. Patients not treated with corticosteroids had low plasma zinc and high plasma copper, which significantly correlated with inflammatory parameters (erythrocyte sedimentation rate and C-reactive protein); no change was observed in erythrocyte and urine zinc and copper. Oral long-term treatment with corticosteroids (less than 10 mg prednisolone/day) was associated with a further decrease in plasma zinc only in patients with moderate inflammation. Changes induced by such a treatment were less intense than those due to severe inflammation. The administration of intravenous high doses (1 g methylprednisolone/day) resulted in a rapid decrease in plasma zinc with a return to baseline levels two days after withdrawal. The modification was associated with a sustained increase in urine zinc and copper without changes in diuresis. | |
| 1911537 | HLA-DR4Dw4-restricted T cell recognition of self antigen(s) in the rheumatoid synovial com | 1991 Jul | The pathogenesis of joint destruction in rheumatoid arthritis remains ill defined, although it is thought to be the result of tissue damage mediated by T cells. This prompted us to isolate and characterize in vivo activated T cells from rheumatoid arthritis synovial fluid in an attempt to determine their specificity. Heterogeneous synovial fluid cells, containing both adherent and non-adherent cell types, were recovered from joint aspirates and cultured in the presence of IL-2. After 2 weeks, the non-adherent cells were phenotyped as CD3-positive and TCR alpha beta-positive T cells. Polyclonal T cell lines were derived from four rheumatoid arthritis patients; of these, two proliferated, in a dose-dependent manner to only autologous synovial fluid in the presence of autologous or DR4Dw4 histocompatible antigen presenting cells. T cell proliferation to the synovial fluid could be inhibited by monomorphic anti-HLA-DR monoclonal antibody, but not by anti-DQ or anti-class I antibodies. T cell clones were established by limiting dilution of a synovial T cell line in the presence of autologous synovial fluid and DR4Dw4 histocompatible accessory cells. Examination of the antigen specificity of these T cell clones demonstrated that they were reactive with a component of synovial fluid. The results of these experiments suggest the presence of an MHC class II-restricted antigen in the rheumatoid arthritis synovial compartment that induces proliferation of in vivo activated T cells. | |
| 2511262 | [Studies on the blood circulation at the proximal site of the tibia in rheumatoid arthriti | 1989 Sep | It has not been well studied the blood flow of the bone of patients with rheumatoid arthritis. For this reason, the author of this report studied the circulatory state of the venous system at the proximal portion of 28 tibiae from 22 patients with rheumatoid arthritis and one from a healthy male as a control. In this study, we tried to correlate the clinical symptoms with local destruction of the joint and the bone atrophy. We injected 1 ml of saline into the medullary cavity through the cortical bone, then measured the time required for the back-flow of 5 drops-leakage of bone marrow blood. In addition oxygen pressure and carbon dioxide gas pressure of the marrow blood were evaluated, phlebography was also taken. Delayed venous circulation shown on the phlebogram tended to coincide with high rates of back-flow. When the venous circulation surrounding and within the bone marrow was fair, many of our patients were found to have mild pain associated with gradual degeneration and moderate atrophy of the bone. On the other hand, when the venous circulation of the above area showed stasis, most of our patients were found to have progressive destruction and atrophy of the bone. This study has concluded that inflammation and swelling of the synovial membrane produces circulatory disturbances in and out of the joint. These disturbances result in bone atrophy and the aggravation of clinical symptoms including intensified pain. | |
| 3217068 | 99Tcm-labelled leucocyte imaging in active rheumatoid arthritis. | 1988 Dec | A simplified technique of labelling leucocytes with technetium-99m is described and applied to patients with active rheumatoid arthritis. The clinically active and less active knees in seven patients were imaged and the uptake of labelled leucocytes was measured. The measurements were repeated after local steroid injection into nine painful knees. A 50-80% reduction in leucocyte uptake localized to the region of the synovium was demonstrated in the eight knees which showed clinical responses and a rise of 8% in the non-responder. There was a variable response in the knees that were not injected. 99Tcm leucocyte imaging in rheumatoid arthritis is able to assess objectively joint inflammation and its response to treatment. | |
| 1839749 | [Comparison of patients with rheumatoid arthritis and chronic backache from the aspect of | 1991 | 36 patients with rheumatoid arthritis and 44 patients with chronic low back pain were psychologically tested by the MMPI-201, Self-concept of Invalidism Scale, Impact of Pain on the Patients' Lives Scale, and "Pain Games" Scale. Patients with rheumatoid arthritis show significantly higher values at the depression and hypomania scale, while those with chronic low back pain manifest significantly higher values at the hysteria scale of the MMPI-201. This latter group also manifests significantly higher values at the Self-concept of Invalidism Scale and at the "Pain-Game" Scale. | |
| 1757944 | Soluble interleukin-2 receptor in juvenile rheumatoid arthritis. | 1991 Sep | Juvenile rheumatoid arthritis (JRA) is a chronic, relapsing, inflammatory childhood disease characterized by arthritis and systemic inflammation. At present there is no rapid, efficient laboratory method of assessing disease activity and degree of immune activation. We measured serum soluble interleukin 2 receptor (sIL-2R) levels in 85 samples from 72 patients (22 samples from patients with systemic JRA, 34 from polyarticular patients, 29 from pauciarticular patients, of which 10 were HLA-B27 positive). The mean sIL-2R level from patients was 1565 U/ml, which is significantly elevated compared to control values of 594 U/ml (p less than or equal to 0.005). The highest levels were seen in patients with systemic JRA (mean value 2121 U/ml) while the lowest values were seen in HLA-B27 positive (+) patients (mean value 899 U/ml). Patients with clinically active disease had significantly elevated levels (mean value 1745 U/ml) compared to patients with inactive disease (mean value 846 U/ml, p less than or equal to 0.01). Highest levels were seen in patients with active systemic JRA (mean value 2419 U/ml) while patients with pauciarticular JRA and B27 + JRA had the lowest sIL-2R levels (1167 and 1045 U/ml, respectively). sIL-2R levels were elevated in all subgroups of clinically active patients compared to controls (p less than or equal to 0.0005). Three of the 4 patients with serial sIL-2R measurements showed falling values during the period of clinical remission. Using regression analysis and likelihood ratio tests, we found a significant correlation between sIL-2R levels and both disease activity and joint count (p less than or equal to 0.005).(ABSTRACT TRUNCATED AT 250 WORDS) | |
| 3401649 | Observations on the causes of rheumatoid arthritis. | 1988 | Inflammatory, symmetrical peripheral polyarthritis is observed in a small proportion of patients following a variety of parasitic, viral and microbial infections. The development of such a post-infective arthritis disorder may depend on a sequence of events, which need not be confined to one extrinsic pathogenic organism. | |
| 2967945 | Prediction of pain behavior and functional status of rheumatoid arthritis patients using m | 1988 Apr | This study examined the extent to which the psychological variables of depression, anxiety, and helplessness predicted the pain behavior and functional status of 64 rheumatoid arthritis (RA) patients beyond what could be predicted on the basis of demographic and medical status variables. Pain behavior was evaluated using a standardized observation method, and functional status was assessed using a modified Health Assessment Questionnaire (MHAQ) and rheumatologists' ratings. Regression analyses revealed that a modified rheumatoid activity index and/or disease duration were significant predictors of levels of guarding, rigidity, and total pain behavior. The psychological variables examined did not predict independently RA pain behavior. The rheumatoid activity index explained a significant proportion of the variance in functional status ratings and MHAQ daily function scores. Age, disease duration and depression also were independent predictors of functional status ratings. Thus, depression had a significant relationship with physician ratings of functional status but not with patient self-reports of disability. Psychological factors not examined in this study that might influence RA pain behavior and self-reports of functional status are discussed. | |
| 2095004 | Rheumatoid arthritis may be primarily associated with HLA-DR4 molecules sharing a particul | 1990 Nov | Genomic typing of in vitro amplified DNA with sequence-specific oligonucleotide (SSO) probes was performed for DRB1, DQA1, DQB1, DPA1 and DPB1 alleles in 54 random Norwegian rheumatoid arthritis (RA) patients and 181 healthy controls. DRB1 alleles encoding the serological specificity DR4 were found in 80% of the patients, compared to 34% of the controls (relative risk = 7.9, p less than 0.0001). All DR4-positive RA patients carried either DRB1*0401 (Dw4), 0404 (Dw14), or 0405 (Dw15), while no patients were found to carry DRB1*0402 (Dw10) or 0403 (Dw13). The frequency of the DRB1*0101 allele encoding DR1 was not increased, even among DR4-negative RA patients, and we were unable to detect any sharing of other class II alleles among DR4-negative patients. No contribution of any DQA1, DQB1, DPA1 or DPB1 alleles to RA susceptibility could be detected. The results suggest that in the Norwegian population RA is primarily associated with a shared sequence at residues 67-74 of the DR beta 1 chain, but only when this sequence is expressed on DR4 molecules. | |
| 3251351 | Synovial processes in rheumatoid arthritis. | 1988 | The clinical feature of rheumatoid arthritis (RA) is characterized by systemic-immunological, local-inflammatory phenomena. But it is the joint destruction which gives RA its dramatic course. Through the years we evaluated joint tissues of app. 14,500 patients with defined RA and besides the conventional inflammatory processes we could prove a mechanism which is responsible for the joint destruction and which is typical for RA. Following an exudative episode, compact, homogeneous cell masses can occur in the synovial membrane which consist of macronuclear, immature, synoviogenous cells. These masses can encroach on the adjacent structures of articular cartilage and subchondral bone which consequently enzymatically will be degraded and destroyed. Since these rapidly growing cell masses are avascular in their aggressive stage, they soon will collapse. The surviving cells "modulate" to fibroblast which start collagen synthesis and thus form the well-known pannus. In the area of the compact, homogeneous cell masses of synovial origin, there are no lymphocytes and plasma cells nor PMN or macrophages. Macrophages only occur after the breakdown of the cell masses and the beginning of pannus formation, this also is the case with lymphocytes and plasma cells. Thus, the immature synoviogenous cell masses are in contrast to the initial synovitis not of inflammatory character. Their cytological and aggressive behavior rather shows oncological analogies. This also corresponds to our proof of the expression of myc and ras to a high degree in the aggressive cell masses in RA. |