Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 3533338 | Disease distributions in patients with multiple patterns of nuclear staining detected by F | 1986 Jul | The present study examines disease distributions in patients with multiple nuclear immunofluorescent staining patterns detected by FANA testing. Among 4003 consecutive patient sera examined, we found that 813 yielded conventional well-defined single staining patterns, while 46 produced multiple pattern combinations. Homogeneous plus nucleolar was the most prevalent combination, and 5 other combinations were identified. Multiple FANA patterns occurred independently of FANA titers and antibodies to dsDNA and Sm. Compared to control patients with single FANA patterns, patients with multiple patterns were found to have an increased frequency of SLE and diseases of the scleroderma spectrum (DDS) while rheumatoid arthritis (RA) and non-immunologic disease were reduced in frequency. These specific correlations indicate that recognition of multiple FANA patterns adds useful diagnostic information to existing antinuclear antibody testing procedures. | |
| 3465105 | [Relation between pain experience and psychological markers in patients with fibrositis sy | 1986 Jul | We attempted to ascertain to what degree pain experience in two groups of rheumatic patients is influenced by personality traits and psychosocial characteristics. 20 patients with fibrositis syndrome and 20 patients with rheumatoid arthritis completed the Freiburger Personality Inventory, the Giessen Test and the Revised Multidimensional Pain Scale. There was no difference in mean scores of the three questionnaires between the groups but patients with fibrositis syndrome tend to characterize themselves as being psycho-somatically disturbed. The number of significant correlations between pain scales and the personality scales is distinctly smaller in the fibrositis group than in the arthritis group. This result is interpreted as an indication for alexithymia in the fibrositis group. | |
| 2835019 | T cell cytotoxicity to Epstein-Barr virus infected B cells: comparison of patients with rh | 1988 Apr | Specific T cell cytotoxicity to Epstein-Barr virus (EBV) infected B cells is reported to be abnormal in rheumatoid arthritis (RA). The regression phenomenon was used to determine whether the immunoregulatory defect in RA is restricted to T cells, B cells, or HLA type. Peripheral blood T and B cells from patients with RA and their HLA identical healthy siblings were mixed in varying ratios with and without EBV, and thymidine incorporation was measured on days 7, 14, and 21. The results suggest that the T cell abnormality is related to disease activity and that an inherent defect exists in the rheumatoid B cell which is independent of disease activity. | |
| 3948434 | Increased binding of D-penicillamine to monocytes in rheumatoid arthritis. | 1986 Apr | Long-term therapy of D-penicillamine (D-Pen) for rheumatoid arthritis (RA) is associated with a fall in rheumatoid factor, but many patients develop autoantibodies. In vitro binding of D-Pen to human peripheral blood monocytes was examined in 37 patients with RA and 75 healthy subjects. Mononuclear cells were reacted with D-Pen coupled to a fluorescein isothiocyanate-bovine serum albumin (BSA) conjugate in the presence of sodium azide and BSA, and analyzed by flow cytometry. Patients showed significantly higher D-Pen binding to monocytes than did healthy subjects. The proportion of monocytes binding D-Pen increased with age in the patients but not in healthy subjects. None of 6 patients who had D-Pen-induced autoimmune side effects was associated with increased D-Pen binding though patients with therapeutic responses showed high D-Pen binding. These results suggest that D-Pen binding to monocytes may be important in mediating therapy and inducing autoimmune side effects. | |
| 2912467 | The beneficial outcomes of the arthritis self-management course are not adequately explain | 1989 Jan | Evaluation of the Arthritis Self-Management Course revealed significant positive changes in the practice of behaviors that were taught and in health outcomes. However, utilizing a variety of statistical techniques, we were able to demonstrate only weak associations between changes in behavior and changes in health status. This suggests the need to examine the mechanisms by which health education affects health status. | |
| 1851075 | Differential stimulation by oxygen-free-radical-altered immunoglobulin G of the production | 1991 Apr | 1. The effect of free-radical-altered IgG (monomer and polymer u.v.-irradiated IgG), compared with that of native and heat-aggregated IgG, on the production rate of superoxide anion and hydrogen peroxide by granulocytes (polymorphonuclear leucocytes) from normal blood and granulocytes obtained from the blood and synovial fluid of patients with rheumatoid arthritis was studied. 2. Similar rates of superoxide production by granulocytes from normal blood at rest and in the presence of any form of IgG were found. In contrast, the rate of hydrogen peroxide production could be stimulated in a dose-dependent fashion by monomer or polymer u.v.-irradiated IgG. 3. The stimulatory effect of free-radical-altered IgG on the rate of hydrogen peroxide production did not occur in the presence of 2-deoxyglucose, which deprives the NADPH:O2 oxidoreductase of its substrate NADPH by inhibition of glycolysis and the pentose phosphate pathway. This points to a stimulatory effect on the direct divalent reduction of oxygen without intermediate superoxide production by this enzyme complex. 4. Granulocytes obtained from the blood and synovial fluid of patients with rheumatoid arthritis reacted differently to polymer u.v.-irradiated IgG. In the presence of this stimulus the rate of release of both superoxide and hydrogen peroxide was increased. Furthermore, these granulocytes synthesized superoxide and hydrogen peroxide at a higher rate than did granulocytes from normal blood in the presence of serum-treated zymosan but not in the presence of phorbol myristate acetate. 5. Taken together, these results indicate that the rate of superoxide and hydrogen peroxide production by the granulocyte NADPH:O2 oxidoreductase depends on the pathological condition of the donor and the type of stimulus used. | |
| 1723811 | Effect of gold therapy on CD5+ B-cells and TCR gamma delta+ T-cells in patients with rheum | 1991 | Circulating CD5+ B-cell levels in 15 patients with rheumatoid arthritis (RA) not receiving remittive therapy was significantly increased when compared to 17 normal controls (mean +/- SE: RA, 19.7 +/- 2.85%; controls, 11.6 +/- 1.67%; P less than 0.02). In contrast, 24 patients with RA receiving gold sodium thiomalate therapy (GST) had similar CD5+ B-cell levels (11.88 +/- 1.65) when compared to controls and significantly reduced levels when compared to the RA group not receiving remittive agents (P less than 0.01). Furthermore, TCR gamma delta+ T-cell levels were also assessed in these patients groups. These values were not significantly different between any of the groups (controls, 4.46 +/- 1.36%; GST, 6.88 +/- 1.73%; RA, 2.73 +/- 0.55%), although 42% of the GST treated group had gamma delta+ T-cell levels higher than the entire untreated RA group. No correlation was observed between the levels of TCR gamma delta + T-cells and CD5+ B-cells in any of these groups. These results suggested that therapy does influence the level of CD5+ B-cells and gamma delta+ T-cells in these patients. | |
| 2264912 | [Immuno-diagnostic findings in "secondary" Sjögren syndrome in chronic polyarthritis]. | 1990 Nov | In order to characterize typical findings in patients (pts.) with secondary Sjögren's Syndrome (SS) associated with rheumatoid arthritis (RA), 173 patients with RA were studied. Pathological screening-test results (Schirmer- and Saxon-test) were followed by ENT- and ophthalmological investigations and examinations in the field of internal medicine. Secondary SS associated with RA was diagnosed in 34 pts. (20%). Immuno-serological SS-A and/or SS-B-antibodies were found in 11 (32%) of 34 patients. 9 pts. (27%) had antinuclear antibodies. Abnormal titers of circulating immune complexes were present in the sera of 24 pts. (71%), salivary gland-antibodies in 3 pts. (9%). In 16 of 34 pts. (47%) minor salivary gland biopsy was performed. Lympho-plasma cell sialadenitis could be verified in all pts. Immuno-histological reactions to anti-IgG and/or anti-IgM and/or anti-IgA were detected in 10 (62%) of 16 pts. We could not find a single criterion to ascertain the diagnosis SS which still has to be made by considering all clinical and serological findings. Immunological findings may provide helpful and interesting diagnostic aspects which by long term observation may even gain importance; however, no differential therapeutic consequences can be seen at the moment. | |
| 2473844 | Characteristics of tumor necrosis factor production in rheumatoid arthritis. | 1989 Aug | The biological effects of tumor necrosis factor (TNF) include the enhancement of fibroblast proliferation, the secretion of collagenase and prostaglandin E2 (PGE2) by fibroblasts, and the resorption of bone and cartilage, suggesting a role for this cytokine in arthritic conditions. To investigate this, we measured the levels of TNF in synovial fluids and evaluated its secretion by synovial fluid mononuclear cells and tissues from patients with rheumatoid arthritis, osteoarthritis, and seronegative arthritis and normals. TNF was found to be secreted in all arthritic conditions but not in normals. The levels of TNF were highest in synovial fluid and correlated with interferon-gamma (IFN-gamma) levels but not PGE2. The production of TNF was stable in a single joint for 3 to 6 months. Using immunohistochemical staining, TNF was localized to mononuclear cells in the lining layer, sublining, and perivascular areas of synovial tissue. The secretion of TNF by rheumatoid synovial fluid mononuclear cells was inhibited by PGE2, while IFN-gamma enhanced its production in those cells which were spontaneously secreting TNF. Our data suggest that TNF may play a role in various arthritic diseases. | |
| 2711369 | Haplotypes bearing HLA-A, -B, and -DR: Bf and C4 genes in rheumatoid arthritis families. | 1989 Jan | We have compared haplotypes bearing HLA-A, -B, -DR; Bf and C4 genes in 54 rheumatoid arthritis (RA) and 24 control families. There was no statistically significant differences in C4A or C4B gene frequencies between RA and control groups, although there were trends for C4B*Q0 to be reduced and C4B2 to be increased in DR4 positive RA compared with DR4 positive controls. The lack of any strong association between C4 variants and RA overall makes it unlikely that the association between RA and genes within the MHS represents a direct effect of variants within the C4A or C4B loci themselves. On comparison of DR4-bearing haplotypes, the haplotype B15-BfS-DR4 was increased fourfold and the B44-Bfs-DR4 haplotype was less frequent in the RA group. When C4 variants were also considered, the haplotype B44-C4B*Q0-C4A3-BfS-DR4 was nine times less frequent in RA patients than in controls. The observation that different DR4 bearing haplotypes may confer either increased or decreased susceptibility to RA suggests either that it is unlikely that DR4 itself is involved in the disease process or that specific haplotypic combinations are important. Thirty-two RA patients were HLA-DR4 negative. No single DR4 negative haplotype was found to confer significantly increased susceptibility to RA. | |
| 3229077 | C4 and C5 body vertebral erosions in early rheumatoid disease. | 1988 Sep | Erosions at the antero-superior angles of C4 and C5 developed in 11 patients (22%) with rheumatoid arthritis of less than one year's duration were followed-up over a 10-year period. These erosions do not appear to have been described before. | |
| 3187821 | Rheumatoid arthritis impact survey. | 1988 Oct 15 | A survey was undertaken to determine the effects of rheumatoid arthritis on the daily lives of patients. A randomly selected group of 135 Indians and blacks with rheumatoid arthritis were interviewed. Only 24% were employed and 35% were dependent on financial support from the state. Twenty-two per cent did not know the name of their illness and 89% expressed a desire to learn more about it. The most important problems encountered were pain (96%), stiffness (80%) and financial (58%). Activities such as toilet after-care, combing hair, bathing and dressing presented problems for a significant proportion of patients. The majority (73%) reported improvement on therapy. The survey showed that in rheumatoid arthritis, there is a great need for education and counselling of patients, there is a high unemployment rate and there is also a need for total patient care by a team of health professionals. | |
| 2236626 | [Bactericidal properties of granulocytes and plasma in patients with rheumatoid arthritis | 1990 | 26 patients with rheumatoid arthritis complicate by infection of urinary tract, have been decreased bactericidal activity of granulocytes. No changes has been noted in the bactericidal activity of plasma. These results suggested that neutrophils in rheumatoid synovial membrane plays important role of inflammation processes. | |
| 3521611 | Rehabilitation for rheumatoid arthritis patients. A controlled trial. | 1986 May | For 1 year we obtained questionnaire and clinical measures from 2 cohorts of rheumatoid arthritis patients: 49 experimental patients who were admitted for an average of 13 days to a university-based rheumatology rehabilitation unit, and 43 control patients who received care from rheumatologists associated with another teaching hospital. At 1 year, after controlling for groups differences, the experimental patients demonstrated significant (P less than 0.05) improvement compared with control patients in several functional status, mental health, and disease activity measures. | |
| 2665044 | The therapy with cyclosporin A in rheumatoid arthritis. | 1989 Jan | Cyclosporin A (CyA) was administered in 6 cases of classical or definite, seropositive rheumatoid arthritis (RA), stages II or III, resistant to the antiinflammatory or nonsteroid therapy, or dependent on high Prednisone doses. An intramuscular daily dose of 1.5 g CyA for a three-week period had good clinical and biologic results. The first signs of improvement, occurring after 14 days of therapy, were manifested by a reduction of the articular inflammation, increase in amplitude of the articular mobility, decrease of the morning stiffness and lowering of the ESR rate. The short-term application of this treatment permits the reintroduction of the nonsteroid drugs and a lowering of the daily dose of Prednisone. | |
| 2736835 | Sjögren's syndrome in rheumatoid arthritis and progressive systemic sclerosis. A comparat | 1989 Mar | One hundred and eleven patients with rheumatoid arthritis (RA) and 44 with progressive systemic sclerosis (PSS) were prospectively evaluated for evidence of Sjögren's syndrome (Ss). The diagnosis was established when a patient with a lip biopsy focal lymphocytic infiltration score of greater than or equal to 2+ in Tarpley's scale had keratoconjunctivitis sicca (KCS) and/or xerostomia. Out of 44 RA and 10 PSS patients with positive lip biopsy, 34 and 9 had criteria for Ss respectively, suggesting a 31% prevalence of Ss in RA and a 20.5% in PSS. Six per cent of the RA patients spontaneously offered complaints of subjective xerophthalmia whereas 11.1% and 22.2% of those with PSS did so for subjective xerophthalmia and xerostomia respectively. However, specific questionnaire elicited subjective xerophthalmia in 38.2% and subjective xerostomia in 5.9% of the RA patients, whereas in 55.5% and 66.7% of the PSS ones respectively. Parotid gland enlargement was detected in 20.6% of the RA and in 44.4% of the PSS patients with Ss. Anti-Ro (SSA) antibodies were present in the sera of 23.5% and 33.3% of them respectively. Severe extraglandular manifestations were unusual in both groups. Our results suggest that, although both Ss in RA and that in PSS lack prominent exocrine gland symptomatology, certain differences between the two and similarities of the latter to primary Ss, would not justify the term 'secondary Ss' for the syndrome accompanying scleroderma, as it has been applied to that accompanying RA. | |
| 2735158 | [Detritus synovitis in chronic polyarthritis: a clinical and operation histologic evaluati | 1989 Mar | In rheumatoid arthritis (RA) joint inflammation is due to two processes: 1) the underlying inflammatory process (UIP) characterized by a lymphoplasmacellular infiltration of the synovial tissue, as well as pannus formation, and 2) the detritogenic synovitis (DS), a synovial response to articular wear products from cartilage and bone (detritus) that induces a preferentially fibrinous inflammation. In order to estimate the role of DS in the clinical presentation of such joints, 40 patients with RA undergoing knee-joint surgery on 48 occasions were evaluated for clinical parameters, radiological stage (Larsen), and histopathological characteristics of UIP and DS. The clinical parameters were comparable in knee joints with predominantly UIP or DS. However, DS was regularly seen in knees with advanced destruction according to Larsen's stages 4 to 5, while UIP occurres in joints even without radiological damage. In conclusion, it is assumed that the poor response of patients with advanced RA to so-called long-term drug therapy may be in part explained by the modifying influence of joint detritus on the underlying "rheumatoid" inflammatory process. | |
| 2688074 | Treatment profiles in different groups of RA-sufferers: description, analysis, evaluation. | 1989 | (1) 50 to 80% of all rA-sufferers treated by rheumatologists have received second line therapy in contrast to 7 to 27% at the community level. (2) Disease duration and disease activity do not seem to affect the treatment regimens of primary and other physicians working in or around Hannover. The same holds true for social and demographic characteristics of the patients. (3) RA-sufferers are generally undertreated by community based doctors with regard to RiD-therapy and disease activity. Approximately 80% of patients with formal indication for the use of RiDs have not received these drugs, despite the fact that a rheumatological department with an outpatient clinic providing more than 3500 consultations per year has existed for more than 20 years at the Medizinische Hochschule Hannover. This outpatient clinic is utilized by more than 60% of all general practitioners as well as internal and orthopedic physicians working in the city of Hannover. We thus have to admit a rather low community effectiveness of our service. (4) A sensitivity of the formal evaluation-scheme of 0.70 and a specificity of 0.80 imply 30% false-negative and 20% false-positive assessments. In view of the larger number of false-negative judgements one must assume an even greater difference between treatment-reality and rheumatological concepts. (5) Sofar we cannot offer any safe explanation for the reasons underlying this unsatisfactory situation. The predictors we have investigated up to now have not proven to be selective. (6) Nevertheless, practical steps are urgently required, in order to be rheumatologically more effective at the community level--at least in Hannover/FRG. | |
| 1953230 | Prednisone plus azathioprine treatment in patients with rheumatoid arthritis complicated b | 1991 Nov | The therapeutic effect of prednisone combined with azathioprine was studied in 28 patients with rheumatoid vasculitis. Nine patients with severe systemic vasculitis were treated initially with 60 mg of prednisone and 2 mg/kg of body weight of azathioprine daily. Clinical signs of vasculitis decreased in all patients. Nineteen patients with only cutaneous vasculitis entered a randomized controlled study comparing prednisone plus azathioprine treatment vs continuation of various conventional antirheumatic drugs. Although measures of both vasculitis and arthritis activity improved to a greater degree in the patients treated with prednisone plus azathioprine in the first 3 months of therapy, no significant differences between the results of the two treatments were observed at the end of the follow-up period. Prednisone plus azathioprine treatment was associated with a low incidence of relapse of vasculitis, few serious complications, and a relatively low mortality. We conclude that the combination of prednisone and azathioprine is effective in the treatment of severe systemic rheumatoid vasculitis; rheumatoid vasculitis with only cutaneous manifestations has a relatively good prognosis, and there is probably no indication for therapy specifically directed at the vasculitic process. | |
| 3264697 | Mechanism of anaemia in rheumatoid arthritis: demonstration of raised interleukin 1 beta c | 1988 Dec | The pathogenesis of the anaemia associated with rheumatoid disease is unclear. It has previously been shown that the degree of the anaemia correlates with the severity of the inflammatory disease and that serum from patients with arthritis inhibits erythropoiesis. This study was designed to examine whether interleukin 1 could be a mediator of the anaemia in rheumatoid arthritis. Radioimmunoassay of interleukin 1 beta in serum showed that patients with rheumatoid arthritis and associated anaemia had significantly higher interleukin 1 beta concentrations than patients with rheumatoid arthritis without anaemia. Pure recombinant human interleukin 1 alpha and interleukin 1 beta, in concentration ranges similar to those found in the arthritic patients, markedly suppressed the colony formation of the erythroid, but not the granulocyte-macrophage progenitor cells in cultures of normal bone marrow. Natural human interleukin 1 and recombinant interleukin 1 beta, but not interleukin 1 alpha, suppressed in a dose dependent manner the proliferation of the human erythroleukaemia cell line (HEL) in cultures, suggesting that the interleukin 1 effect is a direct one. The results show that interleukin 1 is a humoral inhibitor of erythropoiesis and suggests that interleukin 1 is involved in the development of anaemia in association with rheumatoid arthritis. |