Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 2254899 | Synthesis and release of phospholipase A2 by unstimulated human articular chondrocytes. | 1990 Oct | High activity of proinflammatory, type II phospholipase A2 (PLA2) was found in synovial fluids (SF) in inflammatory arthritis. In search for the sources of this PLA2, we cultured human articular chondrocytes and cartilage explants from healthy, osteoarthritic and rheumatoid joints. All cultures, unstimulated by cytokines, released PLA2 extracellularly. Cultures obtained from the deep layers of the cartilage released more PLA2 than those obtained from the superficial layers. Deep layer explants released 0.38 to 18.16 pmol/min/mg protein PLA2/day, whereas superficial layer explants released 0.39-3.18 pmol/min/mg/day. Chondrocyte cell cultures continuously released PLA2, in the first day 909-46347 pmol/min/(10)6 cells and after 9-26 days of culture 166-2115 pmol/min/10(6) cells. PLA2 released from chondrocytes was calcium dependent and had optimum activity at pH 7.5. Cycloheximide markedly inhibited its release. Chondrocyte cultures also released muramidase (LZM) but there was no correlation between PLA2 and LZM release. It may be concluded that cytokine unstimulated human articular chondrocytes synthesize and release PLA2 extracellularly which is similar to that found in the SF. Thus, chondrocytes may possibly serve as one of the sources of intraarticular PLA2. | |
| 2490006 | [Caries incidence in rheumatic patients]. | 1989 Jun | The research concerned 97 patients of both sexes, aged from 17 to 89 years, with the history of rheumatic disease, who were treated over the period from 1 year to 24 years by steroid or nonsteroid anti-inflammatory drugs (NSAIL) or analgesics only. The aim of the study was to evaluate the frequency of caries in rheumatic patients treated by corticoids using Klein-Palmer parameters (DMF) and to compare them to the group that was administered NSAIL or analgesics. Student's test of proportions was used in the explanation of the obtained results. A statistically significant higher proportion of carious and extracted teeth was found. The same was found in relation to the number of parameters in patients treated by corticoids in comparison to those treated with NSAIL and analgesics. The majority of female patients was registered in all three groups. The values were slightly higher in patients treated by corticoids than in those who received analgesics only. | |
| 1657008 | Expression of metalloproteinases and metalloproteinase inhibitor in human arthritic synovi | 1991 Sep | The expression of messenger RNA encoding neutral metalloproteinases and the tissue inhibitor of metalloproteinases (TIMP) in human arthritic synovium was evaluated in situ, using RNA probes. Interstitial collagenase and stromelysin were expressed by synovial lining cells in patients with active rheumatoid arthritis (RA). Proteinase messenger RNA was found both in cells expressing mononuclear phagocyte antigens and in cells that were negative for the antigens. TIMP was also expressed predominantly along the synovial lining layer. In highly inflammatory RA, TIMP expression appeared less intense than that of the proteases. In osteoarthritic synovium, TIMP was expressed at easily detectable levels, whereas the expression of collagenase and stromelysin was less prominent. The balance between expression of the metalloproteinases and of the metalloproteinase inhibitor in synovium appears to be altered during inflammation. These results are consistent with the notion that synovium plays different roles in the cartilage damage of RA and of osteoarthritis. | |
| 2948604 | Total lymphoid irradiation of intractable rheumatoid arthritis. | 1986 Dec | Eleven patients with intractable rheumatoid arthritis were treated with fractionated total lymphoid irradiation at a total dose of 20 Gy. Lasting improvement in clinical symptoms was found in four patients during treatment and the remaining patients experienced a similar benefit within 2 months of irradiation. There was marked reduction in exacerbations and the number of joints involved. Morning stiffness, joint swelling and tenderness decreased. Complications included severe fatigue during treatment and acute bacterial arthritis in multiple joints in one patient. Four of the patients have since died, one of renal failure and another of cardiogenic shock following surgery 3 and 24 months after total lymphoid irradiation. Both had generalised amyloidosis. The third patient developed joint empyema and died of toxic cardiac failure. The fourth died 3 months after resection of a Kaposi's sarcoma complicated by wound infection which responded to treatment. Immunologically, total lymphoid irradiation resulted in suppression of the absolute lymphocyte count and a reduction in T-helper cells, while the number of T-suppressor cells remained unchanged. These data provide evidence of T-cell involvement in the pathogenesis of rheumatoid arthritis. Total lymphoid irradiation can induce sustained improvement in clinical disease activity, but severe, possibly fatal, side-effects cannot be ignored. | |
| 3172060 | Anticollagen antibody responses in DBA/1 (H-2q) mice associated with type II collagen-indu | 1988 Aug | We have previously shown that DBA/1 mice immunized with heterologous type II collagen showed remission of the subsequent collagen-induced arthritis (CIA) when pregnant, but experienced exacerbation postpartum. Measurement of anticollagen antibody (aCa) responses by ELISA in primiparous mice immunized at day 1 of pregnancy revealed no significant difference compared to aCa titres in virgin animals, apart from slightly increased titres following the primary immunisation. When mice received collagen challenge during early pregnancy, however, the date at which maximal antibody titres was reached was delayed by 5 days. Pregnancy initiated following the intraperitoneal boost caused a ten-fold suppression in aCa titres with a rise post-partum. Measurements of aCa levels in individuals which showed fetal resorption indicated that suppression of humoral responses was dependent on the presence of a viable conceptus. Antibody titres declined in all animals after a period of time, which was more prolonged in multigravidae where aCa titres were higher than in nulliparous and primiparous mice. The results show that although pregnancy alters aCa responses during the course of gestation, no long-term modification of humoral immunity occurs, an observation in agreement with the clinical findings in these mice and in humans. | |
| 3675661 | Glutathione peroxidase activity in patients with rheumatoid arthritis and in normal subjec | 1987 Oct | The effects of dietary supplementation with selenium were studied in 6 patients with severe, active rheumatoid arthritis (RA) and in 6 healthy control subjects. Initial concentrations of Se in red blood cells and in serum, and the activity of the Se-dependent enzyme glutathione peroxidase (GSH-Px) in red blood cells, serum, and granulocytes were significantly lower in RA patients compared with controls. During Se supplementation, however, the differences in Se levels and in GSH-Px activity between the 2 groups disappeared, except that, in RA patients, GSH-Px activity in granulocytes increased but remained significantly lower than in controls. | |
| 2510739 | Immunoglobulin kappa genotype confers risk of rheumatoid arthritis among HLA-DR4 negative | 1989 Nov | Because DNA polymorphisms of immunoglobulin kappa confer risk for rheumatoid arthritis (RA) and not all persons with RA have the HLA-DR4 marker, genomic polymorphisms of immunoglobulin kappa and HLA-DR beta were determined in white patients with RA. Compared with control subjects matched for DR beta genotype, the homozygous genotype of the constant segment of immunoglobulin kappa (C kappa) was more frequent in the subgroups of RA patients without the DR beta genotype corresponding to HLA-DR4 (relative risk 6.2, P less than 0.01) and patients without DR4 or DR1 (relative risk 6.7, P = 0.013), but not in the DR4+ RA subgroup. Therefore, RA may be a genetically heterogeneous disease, with HLA-DR4 marking one genetic subset and the homozygous C kappa genotype marking another. | |
| 2890243 | [Drug treatment of rheumatoid arthritis and osteoarthritis]. | 1987 Aug 1 | A review is given about more recent methods for therapy of the rheumatoid arthritis as well as of the osteoarthrosis. Of a large importance in the treatment of the rheumatoid arthritis and of the osteoarthrosis is the method of clinical tests of non-steroidal antirheumatic drugs and of the so-called disease-activity modifying substances. Own experiences and results of controlled, clinical open and double-blind studies were given. | |
| 1837860 | ["Rheumatism"--pathophysiologic principles]. | 1991 Aug | Joint diseases can be divided into inflammatory and non-inflammatory diseases as well as conditions with tumorous tissue proliferations. Inflammatory diseases primarily affect the synovial membrane; secondary cartilage and bone may be involved. Non-inflammatory diseases such as osteoarthrosis initially affect the cartilage; however, in the course of the disease an involvement of the synovial membrane and bone occurs. Juxta-articular ischaemic necrosis is complicated by a loss of cartilage and even a synovial reaction. Tumorous proliferations of the synovial membrane like pigmented villonodular synovitis and chondromatosis may also be complicated by cartilage and bone disturbances. It is the aim of this review to give an insight into the articular reactions that occur in these different disease processes. | |
| 1994911 | Local infectious complications following large joint replacement in rheumatoid arthritis p | 1991 Feb | We performed a 10-year retrospective analysis of the frequency of local postoperative infectious complications in methotrexate (MTX)-treated rheumatoid arthritis patients who underwent total joint arthroplasty. Sixty patients, who had a total of 92 joint arthroplasties, were receiving MTX. A comparison group of 61 patients with a combined total of 110 total joint arthroplasties were not receiving MTX. The 2 groups were compared for the occurrence of local postoperative infectious complications and poor wound healing. Eight patients in the MTX group experienced a total of 8 complications (8.7% of procedures). In comparison, 5 patients in the non-MTX group experienced a total of 6 complications (5.5% of procedures), a difference that was not statistically significant (chi 2 = 0.816, P = 0.366). Statistical analysis of many other variables revealed none that could be identified as risk factors for postoperative complications. These results suggest that treatment in the perioperative period with weekly low-dose pulse MTX does not increase the risk of local postoperative infectious complications or poor wound healing in rheumatoid arthritis patients who undergo total joint arthroplasty. | |
| 3778540 | Assessment of the potential pathogenicity of type II collagen autoantibodies in patients w | 1986 Nov | IgG subclass analysis by enzyme-linked immunosorbent assay of the autoantibody to native type II collagen, detected in 9 patients with classic or definite rheumatoid arthritis, demonstrated a predominance of IgG3 autoantibody. Gm allotyping revealed no obvious association with a particular phenotype. In comparative studies, IgG antibodies to the capsular polysaccharides of pneumococci and tetanus toxoid protein in these same patients consisted predominantly of IgG2 and IgG4. Purified type II collagen autoantibody from 3 of these patients activated complement C5 to C5a when bound to human cartilage in vitro, as measured by radioimmunoassay. These results represent direct evidence of a potential pathogenic role for this autoantibody in rheumatoid arthritis. | |
| 2004398 | [Rheumatoid factor in rheumatoid arthritis in old age]. | 1991 Jan 25 | Rheumatoid factors (RF) detected by agglutination methods (latex fixation test, haemagglutination test) were found in a group of patients with rheumatoid arthritis which started in advanced age (RAVV) less frequently than in a group with RA in middle age (RAMV). The author describes the ELISA method for assessment of RF which makes it possible to estimate immunoglobulin classes RF (IgM-RF, IgA-RF, IgG-RF). By this method RF were detected in class IgM in 15% of patients and in some immunoglobulin classes in 20 per cent where are RF negative when assessed by agglutination methods. The ELISA method detects probably also so-called "hidden" RF. In the group of healthy subjects above 60 years at least one class of RF rises to 17.5%. On correlation with the clinical status an association was found with the degree of affection according to the functional score, with the presence of rheumatoid nodes and muscular affection in RA. Patients with RA treated with Methotrexate and Sulfasalazine tend to have declining IgG RF levels. | |
| 1756690 | Evaluation of phenytoin in rheumatoid arthritis--an open study. | 1991 | In an open study, thirty-five patients with active rheumatoid arthritis were treated with phenytoin. Various clinical and laboratory parameters showed good improvement after phenytoin. Early morning stiffness was significantly reduced after 8 weeks (p less than 0.05); pain index (p less than 0.001), articular index (p less than 0.01) and grip strength (p less than 0.01) showed significant improvement after 24 weeks. There was significant reduction in erythrocyte sedimentation rate (p less than 0.01). Except for three patients (skin rash in one and gastric irritation in two), all others tolerated phenytoin well. The results of the present study support the usefulness of phenytoin as a disease-modifying drug for rheumatoid arthritis. | |
| 2165206 | [Radiosynoviorthese in the treatment of patients with rheumatoid arthritis]. | 1990 Jul | Radiosynoviorthese, a new method for the treatment of patients with rheumatoid arthritis, was developed. Altogether 260 patients with rheumatoid arthritis were treated. The therapeutic activity of radioactive colloid Au was administered intra-articularly to all the patients. Indications and contraindications for radiation therapy of rheumatoid arthritis were developed. Good short- and long-term results were noted in most of the patients after radiation therapy. Radiosynoviorthese as a method of local active therapy of affected joints with colloid Au in the multiple modality treatment of rheumatoid arthritis is effective; its prolonged stable therapeutic effect in patients is observed. | |
| 2106770 | Measuring costs and financial benefits in randomized controlled trials. | 1990 Mar | Economic assessment can be incorporated into clinical trials to evaluate and compare the costs and benefits of different health care programs. In this article the three main types of evaluation are discussed: cost-effectiveness analysis, cost-utility analysis, and cost-benefit analysis. The measurement of direct and indirect costs is described and specific examples are quoted. Full economic analyses are given for the use of naftidrofuryl in the treatment of acute cerebral hemisphere infarction and the use of auranofin in the treatment of rheumatoid arthritis. Economic evaluation is seen to be justified whenever a more expensive treatment is expected to produce greater benefit. Such analyses should consider quality of life and health status, as well as the more easily identifiable outcomes. | |
| 2200359 | Raised plasma renin and prorenin in rheumatoid vasculitis. | 1990 Jul | The value of plasma renin and its inactive precursor, prorenin, were examined as a marker for vasculitis in rheumatoid arthritis (RA). Plasma renin and prorenin rise when the renin-angiotensin system is activated; an isolated increase of prorenin may be a marker for microvascular complications in diabetes mellitus. Renin concentrations in plasma obtained from 34 patients with RA (seven with vasculitis, 27 controls) were measured under standard conditions, before and five days after stopping non-steroidal anti-inflammatory drugs; creatinine clearance was also measured. At first the median renin concentration in the patients with vasculitis was 19 (range 12-63) mU/l (normal less than 61 mU/l) and in the controls 9 (3-43) mU/l. The median prorenin concentration in patients with vasculitis was 233 (144-428) mU/l (normal less than 358 mU/l) and in the controls 144 (25-364) mU/l. Renin and prorenin concentrations increased significantly in both groups after withdrawal of nonsteroidal anti-inflammatory drugs. The creatinine clearance was similar in both groups and did not correlate with renin concentrations. In conclusion, it was found that, unlike patients with diabetes mellitus, patients with RA with vasculitis had slightly raised concentrations of both renin and prorenin. These findings signal activation of the renin-angiotensin system and might indicate early cardiac or renal involvement by vasculitis. | |
| 2679014 | Methotrexate in the treatment of rheumatoid arthritis. | 1989 Oct | Methotrexate is an effective agent for the treatment of rheumatoid arthritis. It is now widely prescribed for patients who have not tolerated or responded to gold compounds or penicillamine. Minor adverse reactions are common, and fatal pulmonary toxicity or cirrhosis can occur. The drug does not produce disease remissions, but continued administration helps reduce pain, stiffness and swelling. Within the past year, the Food and Drug Administration has approved methotrexate for use in treating rheumatoid arthritis. | |
| 3094463 | A three year follow up of patients allocated to placebo, or oral or injectable gold therap | 1986 Sep | Ninety patients randomly allocated to receive auranofin, matching placebo, or sodium aurothiomalate have been followed up for three years. Inefficacy led to cessation of treatment in 14 patients receiving auranofin, 27 receiving placebo, and one receiving sodium aurothiomalate. Twenty seven of the patients receiving placebo were reallocated within the study and 16 continued therapy at three years. This group showed similar statistically significant improvement in clinical and laboratory parameters at one, two, and three years to those on an active drug from the outset. Patients who discontinued auranofin because of inefficacy were offered sodium aurothiomalate therapy--eight patients in this group completed three years of treatment on sodium aurothiomalate and showed significant improvement in some but not all parameters. A hand radiograph erosion score showed a deterioration in 80% of patients remaining on auranofin, 75% of those on sodium aurothiomalate, and 80% of the original placebo group who continued an active drug for three years. Although more patients discontinued auranofin over the study period because of inefficacy, no difference could be shown between the degree of improvement in the subgroup who remained on auranofin and those receiving sodium aurothiomalate. No disadvantage in outcome could be shown for patients originally assigned to placebo. | |
| 1765971 | Rheumatic diseases in Alaskan Indians of the southeast coast: high prevalence of rheumatoi | 1991 Oct | A review of rheumatic diseases in the southeast coastal Indians of Alaska revealed high frequencies of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Both prevalence and incidence rates of RA were significantly higher and the peak age of incidence was younger in the southeast Alaskan Indian population than in Alaskan Eskimo groups and the United States population in general. The prevalence of SLE in the Alaskan Indian population was about twice that reported for most white populations. The frequency of seronegative spondyloarthropathic disorders was similar in the Alaskan Indian and Eskimo populations. Comparable studies of the prevalence of spondyloarthropathy in general have not been carried out in white populations. The prevalence rate of ankylosing spondylitis, one of the major types of spondyloarthropathy, did not differ significantly in the SE Indians from rates in predominantly white US populations. | |
| 1914633 | The 1958 and 1987 ARA revised criteria for rheumatoid arthritis in Chinese patients. A com | 1991 Aug | The American Rheumatism Association (ARA) 1958 and 1987 criteria for the classification of rheumatoid arthritis (RA) were applied to 111 consecutive RA patients and 54 patients with non-RA connective tissue diseases from three hospitals of tertiary level in Beijing. Comparison of the two criteria showed that the specificity was the same, being 88% for both, whereas the sensitivity varied from 94% of the 1958 criteria to 91% of the 1987 criteria. Factors affecting the sensitivity were morning stiffness for more than one hour and increase of number of swollen joints from one to three in the 1987 revised criteria. |