Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 2894204 | Circulating inhibitor bound elastase in patients with ankylosing spondylitis and rheumatoi | 1988 Jan | The plasma concentration of granulocyte elastase in complex with alpha 1 proteinase inhibitor was determined in 42 patients with ankylosing spondylitis (AS) and 33 patients with rheumatoid arthritis (RA). Significantly raised levels of plasma elastase were found in patients with RA, whereas patients with AS had normal values. No correlation was seen between the elastase values and erythrocyte sedimentation rate (ESR), serum haptoglobin, immunoglobulins, or polymorphonuclear cell (PMN) count in either of the patient groups. A correlation was found between the Ritchie index and plasma elastase in patients with RA. After three months' treatment with sulphasalazine a clinical improvement was seen and this paralleled a reduction of the acute phase reaction in both patient groups. A reduction of the circulating elastase values was seen in the patients with RA, whereas no change was seen in patients with AS. | |
| 3954460 | Facial rash with scarring due to granulomatous vasculitis in rheumatoid disease. | 1986 Jan | A 60-year-old man with destructive rheumatoid disease, multiple nodules, and vasculitis developed a facial rash with ulceration and scarring. Histology showed granulomatous vasculitis. The rash was rapidly controlled with oral corticosteroids. Granulomatous vasculitis has not previously been reported as causing this clinical picture in the absence of concomitant necrobiosis and palisading. | |
| 3704508 | [3 cases of obliterating bronchiolitis during treatment of rheumatoid polyarthritis with D | 1986 Jan | The authors report three cases of bronchiolitis obliterans occurring during treatment of rheumatoid arthritis with D-penicillamine after 4, 6 and 8 months of treatment and when the rheumatoid arthritis was in a state of remission. None of these patients (2 sero-positive and 1 sero-negative) present any past history of pulmonary or bronchial disease. The daily dosage of D-penicillamine never exceeded 600 mg. High dose corticosteroid therapy was unable to improve the clinical signs or the respiratory function tests, which were severely disturbed. After 15 months to 3 years, the respiratory signs deteriorated in 2 of the 3 patients. The onset of dyspnea and cough, in the absence of a seasonal infection, in a subject treated with D-penicillamine should raise the possibility of this very serious complication. | |
| 2696291 | [Immunohistochemical studies of the synovial membrane in rheumatoid arthritis and osteoart | 1989 Nov | The exact number of lysozyme- and alpha-1-antitrypsin-positive macrophages in the synovial membrane in rheumatoid arthritis and osteoarthrosis was investigated by the PAP-technique. There is a positive correlation between the number of macrophages in the intima, as well as in the adventitia of the synovial membrane, and the local immunological activity of rheumatoid arthritis. However, there exist significantly less positive macrophages in osteoarthrosis. The significant increase of macrophages in cases of rheumatoid arthritis is an expression of immunopathological mechanisms that have a pathogenetic importance for the induction and recidivity of arthritis. These facts justify therapy with antirheumatica to influence macrophages. | |
| 1781417 | Combined DMARDS in rheumatoid arthritis: past, present and future. | 1991 | The potential for combined therapy in rheumatoid arthritis is rapidly increasing considering the recent development of various new treatment modalities. However, an aggressive effort must be made to stage patients according to various clinical and immunological parameters. Only then can appropriate combined therapy be used effectively in this disease process. The development of such combined therapy could expand into other diseases such as scleroderma, systemic lupus erythematosus and multiple sclerosis. | |
| 2118848 | Phospholipase activity in synovial fluid from patients with rheumatoid arthritis, osteoart | 1990 Jul | Phospholipase activity was assayed in cell-free synovial fluid (SF) from patients with rheumatoid arthritis (RA, n = 28), osteoarthritis (OA, n = 10), and crystal-associated arthritis (C, n = 7) by measuring the release of either [14C]oleic acid or [3H]arachidonic acid from radiolabeled E. coli phospholipids. Activity measured by oleic acid release was not significantly different between the three groups of patients (RA = 571 +/- 43.3, OA = 460 +/- 54.7 and C = 718 +/- 162.6 pmol/min/mg). Arachidonic acid release was significantly (p less than 0.005) less in OA (31 +/- 7.3) than RA (61 +/- 4.7) which was similar to C (58 +/- 17.6 pmol/min/mg). Arachidonic acid release correlated significantly with the SF white blood cell count (r = 0.483, p less than 0.01). This study shows the importance of the type of substrate used to measure phospholipase activity and indicates that differences in the capacity to release arachidonic acid may exist between RA and OA disease states. | |
| 2214637 | [Secondary amyloidosis in elderly patients with rheumatoid arthritis]. | 1990 Jun | Examination of rheumatoid arthritis (RA) patients (52 males and 35 females) revealed secondary amyloidosis (SA) in 44.2% and 48.6% of patients, respectively. The disease was confirmed by amyloid deposits in biopsy specimens of gingival mucosa. As far as age-specific SA incidence is concerned, SA was more prevalent in elderly age-groups: 29.7% and 80% in males under and over 50, respectively; 15.4% and 68.2% in relevant women. The disease history in old patients was not long: 4.6 +/- 1.0 yr and 3.3 +/- 0.7 yr for males and females, respectively. Not a single case of amyloid deposit occurred in patients with systemic lupus erythematosus at the age over 60. The contribution of sex hormones and other factors to SA onset in RA is under discussion. An old age of RA patients and associated infection, especially urogenic, can predispose to SA development. | |
| 3488401 | Mediator and target cell variability in proliferative response of human adherent synovial | 1986 Jun | The proliferation of cultures of adherent synovial cells was studied in response to mediators generated from several sources. Monocyte enriched cultures produced factors in the 70-80,000 and 12-16,000 molecular weight range. Both of these areas of activity also stimulate prostaglandin (PGE) from adherent synovial cells and have interleukin 1 (IL-1) activity. The 70-80,000 region appears to be the 12-16,000 molecular weight activity bound to serum components. When stimulated with the same concentration of a single preparation of this monocyte derived factor, cell lines showed great variability, with inhibition of proliferation in 2 cell lines and the remaining exhibiting stimulation. There was no correlation of proliferation with the PGE producing capacity of the cells. The proliferation response showed no correlation to PGE production by particular cell lines. Lymphocyte enriched cultures produced a factor of 36-40,000 daltons that stimulates adherent synovial cell proliferation but does not have PGE stimulating or IL-1 activity. | |
| 3671222 | Pyogenic infection and rheumatoid arthritis. | 1987 Jan | Ten episodes of severe pyogenic infection occurring in nine patients with rheumatoid arthritis are reported. There was a wide range of presenting features including pyoarthrosis in 7 episodes. Three cases presented with meningitis, bacterial endocarditis and probable multiple abscesses respectively. Infection was caused by Staphylococcus aureus in 7 episodes and by Staphylococcus epidermidis, Streptococcus pneumoniae and beta-haemolytic Streptococcus in each of one episode. Three infective episodes were fatal. Pyogenic, especially staphylococcal, infection should be considered in patients with rheumatoid arthritis with unexplained illness with or without sudden deterioration in joint symptoms. It is important to recognize and treat infection rapidly. | |
| 3108571 | [Gamma 1 heavy chain disease with immune vasculitis and rheumatoid arthritis]. | 1987 Apr 15 | We are reporting about a case of gamma heavy-chain disease (Franklin's disease) with immunovasculitis and rheumatoid arthritis. The diagnosis was confirmed by the results of immunoelectrophoresis of the patient's serum and also by evidence of stimulated lymphocytes without light chain, but having gamma heavy-chain surface proteins. The immunofixation of the serum showed two protein bands of gamma heavy-chains with different loads. These results are confirmed by a two dimensional electrophoresis and isoelectric focussing of the serum proteins. The pathologic protein consists of at least two different heavy-chain proteins (mol wt 40,000 and 80,000) with isoelectric points between pH 5.5 and 7.3. In the urine of the patient pathological gamma heavy chain-protein was found only in a very low concentration. The predominant clinical symptom of the patient was a necrotizing vasculitis which became a therapeutical problem. In the immunofluorescence examination of the skin biopsy specimens, immunoglobulins and C3-complement could be detected in the stratum papillare. This fact would be compatible with the development of antibodies or immune complexes against deposited heavy-chain proteins. The arthropathy and the positive rheumatoid factor could similarly be explained by an immune complex mechanism. | |
| 2658386 | [Arthrocele of the knee joint--a differential diagnosis to deep venous thrombosis]. | 1989 Mar 15 | The symptoms of a thrombosis in deep veins of the calf in patients with rheumatoid arthritis should have thought of the possibility of a synovial cyst or synovial cyst rupture. Because of their too small specificity clinical findings do not allow to decide for one of the two diagnosis. Phlebography and/or arthrography effect the clarification which is necessary for an effective therapy with low risk. Arthrosonography gets increasing importance instead of arthrography. | |
| 2261710 | EPM-ROM Scale: an evaluative instrument to be used in rheumatoid arthritis trials. | 1990 Sep | Different tools are available for the measurement of functional status; however, only a few of them are based on the evaluation of the joint range of motion (ROM). This study is aimed at the design and evaluation of the measurement properties of a ROM scale to be used as an evaluative instrument in rheumatoid arthritis (RA) trials. The EPM-ROM Scale evaluates 10 distinct movements of the small and large joints. The score of each joint varies from 0 (full movement) to 3 (severe limitation) and the cut-off degrees of motion are, in general, based on the lack of ability to perform some determined activities of daily living. The test-retest characteristic of the scale was assessed by administering the scale twice, 5 days apart, to 35 RA patients. The product moment correlation was 0.775 (P less than 0.001). The cross-sectional construct validity of the scale was assessed by the concomitant scoring of the EPM-ROM Scale and the functional ability dimension of the Health Assessment Questionnaire (a reliable, valid and responsive instrument) in these patients. The product moment correlation was 0.518 (P less than 0.001). The evaluation of its longitudinal construct validity and responsiveness are now in progress. | |
| 2663990 | Transforming growth factor-beta production by synovial tissues from rheumatoid patients an | 1989 Aug 15 | The growth of synovial fibroblast-like cells from patients with rheumatoid arthritis and rats with streptococcal cell wall (SCW)-induced arthritis in vitro under anchorage-independent conditions is inhibited by transforming growth factor-beta (TGF-beta). Because this growth factor is present in rheumatoid synovial fluids, we studied whether this cytokine might be secreted by cells in rheumatoid synovial tissue. We show that synovial tissues from patients with rheumatoid arthritis and osteoarthritis, and rats with SCW-induced arthritis, contain TGF-beta-1 mRNA. TGF-beta, predominantly type 1, was spontaneously secreted in vitro by synovial tissue explants and synovial fibroblast-like cells. In addition, TGF-beta could be detected immunohistochemically in cells throughout rheumatoid and SCW-induced arthritic rat synovial tissues. Finally, exogenous TGF-beta induced collagen and inhibited collagenase mRNA levels by cultured synoviocytes. These data support an autocrine role for TGF-beta in the regulation of synoviocytes in rheumatoid arthritis and, in light of its demonstrated effects on the immune system, suggest that TGF-beta might also have important paracrine effects on infiltrating inflammatory cells. | |
| 2805423 | Complement biosynthesis in human synovial tissue. | 1989 Oct | Molecular biological and immunochemical techniques have been used to study the synthesis of complement components by synovial tissue from patients with rheumatoid arthritis or osteoarthritis and by normal synovial tissue from a patient undergoing patellectomy. Using Northern and dot-blot analyses, mRNAs coding for C1-inhibitor, C2, C3, C4 and factor B have been detected, but not for C5. Quantitative analyses of the data have not shown any significant differences in the steady state levels of any of the mRNAs in synovium from rheumatoid arthritis and osteoarthritis patients. When synovial membrane fragments from rheumatoid arthritis, osteoarthritis patients or normal synovium were cultured in vitro, synthesis of C1-inhibitor, C2, C3, C4 and factor B detected by ELISA and C2, C3 and factor B were shown to be functionally active. This study thus provides conclusive evidence that synthesis of complement components occurs locally within normal and inflamed synovial tissue. The local synthesis of complement within normal synovial joints may be of importance in their defence against infection, whereas in inflamed joints it may contribute to the inflammatory response. | |
| 3701726 | Histamine levels in human synovial fluid. | 1986 Feb | Histamine levels have been measured in plasma and synovial fluid (SF) obtained from patients with various arthritides, using a radioenzymatic assay procedure. The concentration of the amine in the plasma of these patients was, in general, found to be considerably greater than that seen in a group of control patients without inflammatory joint disease. SF always contained higher levels of histamine than corresponding plasma samples. The results of our preliminary study suggest that the production of histamine within the synovium may be an important factor in the genesis of joint effusions. | |
| 3324304 | Intestinal bacteria and rheumatic disease. | 1987 | The striking clinical and pathological similarities between certain naturally occurring infectious diseases in animal species and those of some human rheumatic diseases, such as rheumatoid arthritis (RA), have stimulated the search for a microbial etiology of the latter syndrome. A long series of microbial species, including aerobic and anaerobic intestinal bacteria, mycoplasma and several viruses have been put into focus. Most often, however, an initially positive report has been followed by several reports denying an etiological role of the microbial species in focus. However, the concept of a microbial trigger in the etiology and symptomatology of RA is still a subject of intense debate. Recent results have indicated a reversed effect of gram-positive vs. gram-negative intestinal bacteria on adjuvant-induced arthritis in germfree rats and microbial peptidoglycans have been shown to play a major role in this experimental model. It has been shown that the intestinal flora may include bacteria containing antigenic determinant(s) cross-reacting with some markers within the HLA-system. The intestinal flora may also influence upon several digestive and absorptive functions and thereby acting upon parameters of importance in the development of rheumatic disease. | |
| 1987761 | Monoclonal antibodies detect monocyte/macrophage activation and differentiation antigens a | 1991 Jan | Monoclonal antibodies (MAbs) to functionally heterogeneous populations of human rheumatoid arthritis (RA) synovial tissue macrophages and lipopolysaccharide (LPS)-activated U937 cells were generated. These MAbs were used to characterize macrophages in situ in the synovial pannus and to study relative antigen expression on the surface of cells isolated from the synovium and from normal peripheral blood. Monoclonal antibody 3D8, an anti-CD13 MAb, reacts with an antigen expressed on the surface of blood monocytes and is a monocyte activation-related antigen that is upregulated by exposure of monocytes to interferon-gamma (IFN-gamma) and LPS. The expression of the 3D8 antigen increases in parallel with MHC class II antigen expression and also is upregulated in culture as monocytes mature to macrophages. 3D8 antigen is expressed strongly on RA synovial tissue lining cells, which are thought to be composed of macrophages. 8D7 antigen expression, detected by MAb 8D7, increases on blood monocytes on cellular activation with LPS and interferon-gamma, but in contrast to the 3D8 antigen, does not increase with monocyte maturation in vitro. The 8D7 antigen is expressed differentially on density-defined macrophage subpopulations isolated from RA synovial tissue and is expressed more strongly on macrophages that are nonangiogenic than those that are angiogenic. | |
| 1696188 | Relationship between CD5+ B lymphocytes and the activity of systemic autoimmunity. | 1990 Aug | We studied the relationship between CD5+ B cells and the activity of the disease process in patients with autoimmune diseases. In rheumatoid arthritis (RA), levels of CD5+ B cells were associated with autoantibody production as determined by serum rheumatoid factor and antinuclear antibodies. In addition, CD5+ B cells were significantly correlated with C-reactive protein, and data from longitudinal studies showed a marked influence of corticosteroid treatment on numbers of CD5+ B cells. Patients with systemic lupus erythematosus (SLE) had slightly elevated levels of CD5+ B cells as compared with normals, but a close association with measures of an active disease was not observed. In a group of patients with type I diabetes mellitus, CD5+ B cells were detected in patients with anti-islet cell antibodies. Our results suggest that CD5+ B cells are related to the activity of the autoimmune process and can be modulated by therapy in patients with RA. Although CD5+ B cells do not seem to have a major role in SLE, polyclonal activation might affect this B cell subset as well in this disease. Further studies are needed to define the precise role of CD5+ B cells in organ-specific autoimmunity. | |
| 2257451 | A laboratory and clinical study of pneumatic 'grip strength' devices. | 1990 Dec | The use of inflated bags or cuffs to measure grip strength is now a well established technique. The method does have a number of problems. Bags of different diameter and volume were seen to give statistically significantly different pressure readings when squeezed by the same subjects. Different initial pressures (from 20 mmHg to 60 mmHg) also gave significantly different results both in laboratory tests on a materials testing machine and when patients with rheumatoid arthritis squeezed the bag. The technique of squeezing also affected the results. Despite the intrinsic drawbacks of the system, it is likely to remain in general use because of familiarity and convenience. We recommend the minimum details required when pneumodynamometer-derived data are published. | |
| 3673136 | [Catamnestic studies of the significance of synovial membrane needle biopsy for the diagno | 1987 Jul 15 | By means of a catamnestic study diagnostic evidence, number of complications and examination-conditioned stress of the patient caused by needle biopsy of the synovial membrane were tested. 4-11 years after a blind biopsy of the synovial membrane carried out on account of rheumatological indication 80 patients (48 males, 32 females) were examined and the results of the preliminary examination and the secondary examination were compared with the findings of the needle biopsy of the synovial membrane. While the needle biopsy of the synovial membrane in rheumatoid arthritis has a high diagnostic value in comparison to clinical and paraclinical findings, in the histomorphological ascertainment of the uric arthritis the catamnestic study shows the limits of the method due to place-depending differences of the findings of the crystalline deposit in the synovial membrane. The number of complications of the investigation technique was insignificant and had no influence on the duration of the inability to work. As a highly specialised diagnostic method the blind biopsy of the synovial membrane may contribute to the clarification of problem cases in institutions with rheumatological profile without possibility of arthroscopy. |