Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 2724249 | Methotrexate therapy in rheumatoid arthritis: 2-year retrospective followup study. | 1989 Mar | Clinical and laboratory data in 124 patients with rheumatoid arthritis treated with methotrexate (MTX) were retrospectively reviewed over the initial 2 years after the start of treatment. Clinical improvement occurred in 103 (83%) patients after 12 weeks of treatment. At 2 years of followup, 60 patients (48%) continued to receive MTX with sustained clinical benefit. It has been discontinued in 64 (52%) patients (adverse drug reactions in 38, lack of clinical benefit in 15, and miscellaneous reasons in 11). Patients with adverse drug reactions had higher initial serum creatinine and blood urea nitrogen values than patients without adverse drug reactions. | |
| 3029230 | The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B l | 1987 Mar 12 | Rheumatoid peripheral blood mononuclear cells show an increased responsiveness to superinfection with Epstein-Barr virus (EBV). We have investigated the role of adherent cells in this hyperresponsiveness using two different methods of adherent cell depletion. Depletion of adherent cells from both rheumatoid and normal mononuclear cells, using either dextran bead columns or plastic petri dishes, produced inconsistent changes in the response of autologous non-adherent cells to EBV. The addition of supernatants of cultured rheumatoid adherent cells also produced an inconsistent change in response although normal adherent cell supernatants increased the responsiveness of autologous non-adherent cells to EBV. The inconsistencies observed are discussed with respect to adherent cell subpopulations present in rheumatoid and normal peripheral blood mononuclear cell preparations when using recognized methods of adherent cell depletion. | |
| 2357210 | [Patellar changes following knee joint endoprosthesis-plasty]. | 1990 Jan | We analysed the postoperative position of the patella and the degenerative changes in 80 implanted Guepar, Tillmann and GSB-prostheses of the knee. We did not found differences between the types of endoprostheses. We saw many dislocations of the patella, especially in patients with rheumatism, but only some patients had trouble with the operated knee. We can diminish these problems by modelling of the patella and by careful reconstruction of the muscle strength. | |
| 2689490 | A case of bullous pemphigoid induced by tiobutarit (D-penicillamine analogue). | 1989 Aug | A 61-year-old woman with severe rheumatoid arthritis developed bullous lesions on the trunk, conjunctiva, and oral mucosa during treatment with tiobutarit, a new antirheumatic drug analogus to D-penicillamine. Histology showed a subepidermal blister, and circulating antibodies to the basement membrane zone were detected. The bullous lesions improved rapidly after discontinuation of tiobutarit. However, 3 years later, she was re-exposed to tiobutarit and the bullous lesions recurred within a week. This is the first confirmed case of tiobutarit-induced BP. | |
| 3823508 | Recurrent acute renal failure with interstitial nephritis due to D-penicillamine. | 1987 | A 60-year-old man with rheumatoid arthritis, who developed acute reversible renal failure with nephrotic syndrome and tubulointerstitial nephritis in association with multiple-drug therapy, is described. The episode was ascribed to the nonsteroidal anti-inflammatory agent fenbufen, and the patient was reexposed to D-penicillamine within 6 months, reproducing the same renal lesion. There was no evidence of the glomerular lesions characteristically associated with D-penicillamine nephrotoxicity. D-penicillamine was the only drug therapy common to both episodes and it is concluded that it may cause tubulointerstitial nephritis with nephrotic syndrome. | |
| 3264319 | Monoreactive high affinity and polyreactive low affinity rheumatoid factors are produced b | 1988 Dec 1 | In patients with rheumatoid arthritis (RA), circulating CD5+ B lymphocytes, but not CD5- B lymphocytes, are increased in number and size, exist in an activated state, spontaneously proliferate, and secrete Ig that binds to the Fc fragment of IgG. By constructing continuous mAb-secreting cell lines from CD5+ B lymphocytes, the properties and dissociation constants (Kd) of these antibodies were determined. Two types of rheumatoid factors (RFs) with discrete reactivities were produced. The first type is polyreactive and binds with relatively low affinity (Kd, 10(-5) mol/liter) to the Fc fragment of IgG. These antibodies are similar to those produced by CD5+ B cells from healthy subjects. The second type of RF is monoreactive and binds with higher affinity (Kd, 10(-7) mol/liter) to the Fc fragment of IgG. These latter autoantibodies are produced by CD5+ B cells of RA patients, but not healthy subjects. Long-term longitudinal studies are needed to determine the role of these two types of RFs in the pathogenesis of RA. | |
| 2150722 | [Abnormalities in autologous mixed lymphocyte reaction-induced T cell activation cascade i | 1990 Oct | We have previously shown that when patients with active rheumatoid arthritis (RA) were examined for the ability of their lymphocytes to respond in the autologous mixed lymphocyte reaction (AMLR), profoundly reduced AMLR responses were found in the RA patients. The defects were mainly due to the impaired response of CD8+ and CD4+ Law8- subsets; however, both CD4+Leu8+ and CD4+ + Leu8- cells functioned normally as responding cells. The present study demonstrated that the abnormalities of RA CD8+ T cells in the AMLR were corrected when the AMLR cultures were set up in the presence of methyl-B12. In addition, a main target of the methyl-B12 effect observed was both CD8+Leu8+ and CD8+Leu8- cells. Thus, methyl-B12 may become a potential agent that would be able to control the pathophysiology of RA. | |
| 3545105 | Characterisation of nucleolar proteins as autoantigens using human autoimmune sera. | 1986 Dec | Sera from 271 patients with systemic rheumatic diseases were screened for antinucleolar antibodies using immunofluorescence. Antinucleolar antibodies were found in the sera of 73% of patients with progressive systemic sclerosis, 4% of patients positive for autoantibodies with rheumatoid arthritis, and 8% of patients with rheumatoid arthritis associated with sicca complex, but not in patients with systemic lupus erythematosus, Sjögren's syndrome, undifferentiated connective tissue disease, or in healthy donors. The antinucleolar sera (n = 20) were analysed by immunoblotting techniques. In four sera antibodies against nucleolar proteins with molecular weights of 35 kD, 37 kD, 69 kD, 92-93 kD, and 93 kD could be immunodetected. The nucleolar autoantigens were extractable from the nucleoli together with the preribosomal particle fraction and could be solubilised by ribonuclease (RNAse) treatment. Their presence in the nucleolus was sensitive to actinomycin D treatment of cells. The 37 kD autoantigen could be identified as nucleolar phosphoprotein B23. | |
| 3577342 | [Medium-term results following implantation of cemented Weller type endoprostheses]. | 1987 Jan | 102 cement-fixed endoprothesis of Type Weller I, which were followed up for six years on average, showed good results. We saw three aseptic loosenings and one septic loosening (1.5 years after implantation). Circumscribed progressive resorption of the cortical layer is a typical radiological sign of loosening of an endoprothesis. However, radiolucent lines do not prove aseptic loosening. Even stems with a collar may sinter without loosening. No correlation was found between the type of the hip affection (osteoarthritis, aseptic necrosis of the hip, rheumatoid arthritis etc.) and the result of the operation. Regarding our results we consider cement-fixation in total hip replacement as a good procedure in elder patients. | |
| 3619961 | Hyaluronate inhibition of cell proliferation. | 1987 Jul | The proliferation of rabbit synovial cells, 3T3 cells, or simian virus-transformed 3T3 cells in cell culture was inhibited by the addition of hyaluronate to the culture medium. This effect was markedly dependent on the molecular weight and concentration of the hyaluronate. At the molecular weight and concentration of hyaluronate present in normal synovial fluid, proliferation was inhibited. At lower molecular weights or concentrations, as found in rheumatoid synovial fluid, hyaluronate was significantly less inhibitory. Thus, the changes in synovial fluid hyaluronate that are associated with arthropathies may contribute to a favorable environment for rheumatoid pannus expansion. | |
| 3358798 | Arthritis-associated changes in flow cytometric characteristics of cultured synovial fibro | 1988 Mar | Synovial fibroblasts cultured from patients with rheumatoid or reactive arthritis and from controls were studied by flow cytometry, spectroscopy, and electron microscopy. Analysis of 29 cell lines revealed consistent differences between arthritic and normal fibroblasts. Cells cultured from inflamed synovial tissue exhibited higher autofluorescence than did control fibroblasts, and displayed exceptional light scatter properties in flow cytometry, indicating changes in cytoplasmic structures. Electron microscopic examination of the fibroblasts from arthritic synovial tissue revealed large numbers of round, swollen, laminated, mitochondrion-like bodies, which were not observed in the control fibroblasts. The changes observed by flow cytometry (light scatter and autofluorescence) coincided with the presence of the mitochondrion-like organelles. The strong autofluorescence observed in the arthritic fibroblasts resembled the fluorescence spectrum of mitochondrial flavoproteins. These data suggest that persistent metabolic and structural changes have occurred in the mitochondria of synovial fibroblasts and inflammatory synovial tissue. The usefulness of flow cytometry in identifying such cells is described. | |
| 3484935 | HLA haplotype sharing by siblings with rheumatoid arthritis: evidence for genetic heteroge | 1986 Feb | HLA haplotype sharing was studied in 35 sibships in which there were two or more members with rheumatoid arthritis (RA). Haplotype sharing RA siblings was random in 15 sibships which included members with clinical or immunological features of autoimmune thyroid disease. In the remaining 20 'non-thyroid' sibships the frequencies of RA siblings sharing 0, 1, or 2 haplotypes were 0.04, 0.48, and 0.48 respectively (p = 0.006). 67% of RA probands in the 'thyroid' families and 90% in the other families were HLA-DR4 positive. It is suggested that there is genetic heterogeneity in the pathogenesis of RA with at least two independent genes within the major histocompatibility complex (MHC) predisposing to RA. One gene is in linkage disequilibrium with HLA-DR4, while results of comparison of DR antigen frequencies in DR4 negative RA and control groups suggest that the other is in linkage disequilibrium with HLA-DR1 and 3. In the thyroid disease families both genes are frequently present and as either may predispose to arthritis, HLA haplotype sharing is random. The frequencies of HLA haplotype sharing in the 'non-thyroid' families suggest that there is a dominant susceptibility gene in linkage disequilibrium with HLA-DR4, whose frequency is 5% and penetrance about 20%. | |
| 3070764 | The Nottingham Health Profile: an analysis of its sensitivity in differentiating illness g | 1988 | A recent criticism of the Nottingham Health Profile (NHP) claimed it was an insensitive instrument for use in population surveys, because its modal response was zero, and domains measured by the instrument are insufficiently distinct. It is argued here that the NHP was designed specifically to uncover the chronically ill in populations and, further, research undertaken here indicates that the instrument is sufficiently sensitive to be successful in permitting variations within and between illness groups to be determined. However, the domains of mobility and pain, as measured by the instrument, are found to be confounded. | |
| 2758768 | The rheumatoid heel: its relationship to other disorders in the rheumatoid foot. | 1989 Jun | Four-hundred and eight rheumatoid feet are studied. The percentage of talalgia is 3.7%; for inferior exostosis it is 29.6%, and 30.8% for posterior exostosis. Posterior demineralisation is present in 12%, whereas for bony erosion it is 8.1%. Plantar spurs are very closely associated to flattened feet (p less than 0.0001). Likewise a very noticeable statistical association is noted between postero-superior calcaneitis and tarsal arthritis (p less than 0.0001), and calcaneitis was more frequent among marked valgus (p less than 0.001). These results are compared with findings of other works. Therapeutic consequences are evoked. | |
| 2384499 | Perioperative blood loss associated with total knee arthroplasty. A comparison of procedur | 1990 Aug | We reviewed the cases of thirty-four patients (thirty-eight knees) in whom prostheses with an identical design were used for primary total knee arthroplasty, and we compared the perioperative blood loss between the eleven knees in which cement was used and the twenty-seven knees in which cement was not used. The patients who had an uncemented prosthesis had a significantly greater mean blood loss, both intraoperatively (p less than or equal to 0.05) and during each subsequent eight-hour interval on the first postoperative day (p less than or equal to 0.05). The total for the forty-eight-hour postoperative collection also was greater (p less than or equal to 0.01), as was the cumulative loss for the entire study (p less than or equal to 0.01). When patients who had rheumatoid arthritis and osteoarthrosis were considered separately, the results were similar; that is, there was a significantly greater total postoperative blood loss in each of the two groups when cement was not used (p less than or equal to 0.025). A minimum postoperative hemoglobin concentration of ninety-five grams per liter was maintained; a greater percentage of patients (sixteen of twenty-seven) in whom cement was not used needed a transfusion as compared with two of eleven in the group in whom cement was not used (p less than or equal to 0.025), and they also needed more packed red-blood cells (1062 compared with 750 milliliters) (p less than or equal to 0.05).(ABSTRACT TRUNCATED AT 250 WORDS) | |
| 2141120 | [Peripheral inflammatory rheumatism during Horton's disease]. | 1990 May 12 | From a study of the course of Horton's disease in a population of 41 patients followed up for 3 to 14 years, the cases of 5 patients presenting with peripheral inflammatory arthritis were singled out and analyzed. In all five cases, the condition was a subacute, seronegative, symmetrical polyarthritis affecting mostly the wrists, the metacarpophalangeal joints and the knees. In 2 patients radiology showed articular lesions. As in other cases found in the literature, these raise the problem of rheumatoid arthritis-Horton's disease association or true "Hortonian" arthritis. | |
| 2970912 | Phenotypes of peripheral blood T lymphocytes in rheumatoid arthritis and juvenile rheumato | 1988 Jun | The percentage of T4 and T8 positive cells in peripheral blood of 78 patients with rheumatoid arthritis (RA) and 26 patients with juvenile rheumatoid arthritis (JRA) was determined using a rosette technique. The T4/T8 ratio +/- SEM (standard error of the mean) in the RA patients was increased, 2.02 +/- 0.09, as compared with normal blood donors, 1.71 +/- 0.06 (p less than 0.02). The subgroups of RA patients who had a significantly increased T4/T8 ratio were ANA positive patients (p less than 0.02) and patients on no medication (p less than 0.05). In the total group of JRA patients the T4/T8 ratio was 2.01 +/- 0.12 versus 1.75 +/- 0.08 in controls (p = 0.061). Polyarticular JRA patients had an increased T4/T8 ratio as compared with controls (p less than 0.05) while patients with the pauciarticular form had a normal ratio (p greater than 0.10). No correlation between the T4/T8 ratio and a disease outcome index, a laboratory activity index, ESR, WBC, platelet count, hemoglobin, serum albumin, age and disease duration was found (K less than +/- 0.20, p greater than 0.10). | |
| 2059237 | Insufficiency fractures of the distal tibia misdiagnosed as cellulitis in three patients w | 1991 Jul | We describe 3 patients with rheumatoid arthritis who presented with diffuse pain, swelling, and erythema of the distal aspect of the lower extremity, suggestive of either cellulitis or thrombophlebitis, but were found to have insufficiency fractures of the distal tibia. The value of technetium-99m diphosphonate bone scintigraphy in the early recognition of these fractures and a possible explanation for the associated inflammatory symptoms are discussed. | |
| 1796655 | [The role of cytokines and growth factors in rheumatoid joint destruction]. | 1991 Nov | Cytokines and growth factors are important mediators of inflammation and play a major role in both the physiological regulation of bone and cartilage metabolism, and in the destruction of joint-related structures. These complex biological regulatory events have to be regarded as net effects which are dependent on the individual actions of the different cytokines and their corresponding inhibitors in the pericellular environment of the cells present in the inflamed tissues. These effects can be antagonized on various levels by natural or artificial inhibitory molecules. The determination and characterization of cytokines and their inhibitors in body fluids and tissues may contribute to a better understanding of the basic mechanisms of the pathogenesis of inflammatory joint diseases, and may help to develop better modalities of therapy. The objective of the present review is to outline important actions of selected cytokines and growth factors on cells and the surrounding matrix of bone and cartilage in rheumatoid arthritis. It will focus on interleukin-1 (IL-1), IL-1 inhibitors, Tumor-Necrosis-Factor-alpha (TNF-alpha), TNF inhibitors, Interleukin-6 (IL-6), colony-stimulating factors (CSF's), Interferon-gamma (IFN-gamma), growth factors, eicosanoids and prostaglandins, all of which are important in the effector phase of tissue destruction. | |
| 1866571 | Serum IgE anti-cartilage collagen antibodies in rheumatoid patients. | 1991 | The presence of circulating IgG, IgA and IgM antibodies to native cartilage collagens in some patients with rheumatoid arthritis (RA) suggests that an autoimmune response to cartilage collagens may be involved in the pathogenesis of RA. However, the relevance of such antibodies to the pathological process remains unclear, and it is likely that many humoral and cellular derived factors combined to trigger events leading to the chronicity of the rheumatoid lesion. Since histological and biochemical studies have suggested the involvement of mast cells in the rheumatoid joint, we have studied the frequency of IgE antibodies directed against the cartilage collagens type II, IX and XI in patients with active rheumatoid disease. Of the 91 patients' sera tested, 32 had significant levels of IgE anti-cartilage collagen antibodies when compared with non-arthritic controls. Total serum IgE levels did not correlate with the presence of IgE anti-collagen antibodies, nor were any patients positive for IgE antibodies to fibronectin, a widely distributed extracellular matrix component. These results are consistent with an allergic type I hypersensitivity reaction to cartilage antigens in RA involving mast cell and basophil degranulation. |