Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 1921820 | Neutropenia due to low-dose methotrexate therapy for psoriasis and rheumatoid arthritis ma | 1991 Oct 7 | OBJECTIVE: To review experience with neutropenia related to low-dose methotrexate therapy in patients with psoriasis and rheumatoid arthritis. DESIGN: Retrospective review of medical records. SETTING: A 509-bed Melbourne teaching hospital. PATIENTS: Five patients admitted in 1987 and 1988, with neutrophil counts of less than 1 x 10(9)/L, given low doses of methotrexate for psoriasis or rheumatoid arthritis. MAIN OUTCOME MEASURES: Death, or length of hospital admission. FINDINGS: Four patients were women, and one a man; three had been treated for psoriasis, and two for rheumatoid arthritis. Ages ranged from 56 to 91 years. The eldest patients, aged 77, 81 and 91 years, died. The other two were discharged after 43 and 48 days. Prior to or shortly after admission, four patients were treated with penicillin antibiotics which may have interfered with methotrexate excretion. CONCLUSIONS: Methotrexate clearances (related to creatinine clearance rates and presumably low) were probably reduced sufficiently by concomitant therapy to result in neutropenia. Practitioners using methotrexate should be aware of drug interactions resulting in delayed methotrexate excretion. Blood counts should be monitored after changes in therapy, especially in patients with impaired renal function, such as the elderly. | |
| 2584657 | Crossed intrinsic transfer. | 1989 Nov | A retrospective analysis of the long-term results of the crossed intrinsic transfer operation is presented. Twenty-one patients (30 hands) with rheumatoid arthritis and one patient (1 hand) with systemic lupus erythematosus were examined clinically and radiographically. The average follow-up was 12.7 years. The average postoperative ulnar drift for all fingers was 5 degrees. The magnitude of ulnar drift did not increase over time. The average active range of motion for the metacarpophalangeal joints was 47 degrees and for the proximal interphalangeal joints it was 58 degrees. The average radial deviation deformity of the wrist in the resting position was 2 degrees. These variables did deteriorate with time. Extensor carpi radialis longus to extensor carpi ulnaris tendon transfer with crossed intrinsic transfer produced the same result as crossed intrinsic transfer alone. The outcomes for crossed intrinsic transfer attached to the lateral band were similar to outcomes for transfers attached to the collateral ligament of the metacarpophalangeal joint. The crossed intrinsic transfer procedure effectively provides long-term correction of ulnar drift in the rheumatoid hand. | |
| 1976277 | Restriction fragment length polymorphism of a lymphocyte surface antigen, Blast-1, in Japa | 1990 May | Analysis of polymorphic systems, demonstrating differences among ethnic groups, provides a valuable tool for biology and medicine. Blast-1 is a member of the immunoglobulin superfamily and an activation-associated glycoprotein expressed on the surface of mononuclear cells. Blast-1 demonstrates DNA polymorphism in healthy controls and patients with rheumatoid arthritis (RA). The sizes of polymorphic restriction endonuclease fragments of genomic DNA encoding Blast-1 were 2.4 and 1.9 kb. In normal controls, the frequency of the homozygote for the 2.4 kb fragment (L-L) was 0.69 and 0.47, and that for the 1.9 kb fragment (S-S) was 0.04 and 0.11 in Caucasians and Japanese, respectively. The frequency of the heterozygote for both fragments (L-S) was 0.27 and 0.42 in Caucasians and Japanese, respectively. The frequencies of the L and S alleles were 0.83 and 0.17 for Caucasians, respectively, and were 0.68 and 0.32 for Japanese, respectively. The difference in the allele frequency between Caucasians and Japanese was significant. In Japanese patients with RA, the frequency of L-L, L-S and S-S types was 0.45, 0.45 and 0.10, respectively. Lung fibrosis in Japanese RA patients was associated with an increase in the L-S and S-S types and a decrease in the L-L type. The present study indicates that the investigation for gene polymorphisms of Blast-1 among distinct ethnic groups is important because Blast-1 appears to be a genetic marker for the manifestation associated with RA. | |
| 2576478 | Molecular analysis of HLA-D region genes in seropositive rheumatoid arthritis. | 1989 Nov | Analysis of HLA DRB1 and DQB1 Bam HI RFLPs revealed four DRB1 (4.8, 5.2, 6.0 and 7.0 kb) fragments and a 3.2 kb DQB1 fragment to be significantly increased in Caucasians with seropositive RA compared to healthy individuals. The 4.8, 5.2 and 7.0 kb DRB1 fragments were found in 86.5% of RA patients and in 56% of the controls (p = 10(-3), relative risk (RR) = 5.0), while the 6.0 kb fragment was found in 79% of RA patients compared to 32% of controls (p = 2 x 10(-5), RR = 8.0). The 3.2 kb DQB1 fragment was observed in 63.5% of RA patients versus 38.0% of controls (p = 10(-2), RR = 2.8). Analysis of these fragments relative to HLA phenotypes revealed that the 4.8, 5.2 and 7.0 kb DRB1 fragments were strongly correlated with DR4, -7, -9 and -w53 serotypes, the 6.0 kb RFLP with DR4 and the 3.2 kb DQB1 fragment with DR1 and DQw1. Using probes specific for the 5' or 3' regions of the DRB1 gene, the 5.2 and 6.0 kb DRB RFLPs were mapped to the 5' end and the 4.8 and 7.0 kb RFLPs to the 3' end of the DRB1 gene. A probe generated from the second exon of the DRB4 (DRw53) gene recognized only the 5.2 and the 6.0 kb RFLPs corroborating the 5' location of these RFLPs. Family studies further confirmed that these RFLP's segregated with HLA phenotypes. | |
| 1673266 | HLA DQ beta restriction fragment length polymorphism and rheumatoid arthritis. Association | 1991 Mar 16 | Two variants of the HLA-DR4-linked DQw3 allele, namely DQw7 and DQw8, were analysed in patients of mixed ancestry (Cape Coloureds) with rheumatoid arthritis and in healthy individuals from the same population group using a DQ beta-specific cDNA probe. The DQw7 allele, identified by 3,4 kb Hind III or 3,7 kb and 6,9 kb Bam HI DQ beta-specific restriction fragments, was expressed in 93% of DR4-positive patients (N = 15), compared with 12.5% DR4-positive normal individuals (N = 8). This DQ variant showed a highly significant association (relative risk = 98; P less than 0.0001) with rheumatoid arthritis in this population group and may play a role in their susceptibility to this disease. | |
| 1952510 | [Diseases associated with renal amyloidosis. Apropos of 216 cases]. | 1991 | Diseases associated with renal amyloidosis were evaluated in 216 patients observed between 1956 and 1989. The percentage of secondary amyloidosis decreased from 72 to 47%, where it has remained for the past 20 years, despite the clear diminution in the tuberculosis rate; this is due to its increased incidence of association with rheumatoid diseases. Dysglobulinemia was found in more than 75% of the patients with AL amyloidosis. Amyloidosis associated with this dysglobulinemia, of which 30% were myelomatous, represented more than 25% of the cases observed during the past decade and constituted, with rheumatoid arthritis, the two most common etiologies. | |
| 3708903 | Spectrotypic analysis of IgM and IgA rheumatoid factors. | 1986 Mar | The spectrotypes of IgA and IgM rheumatoid factors (RF) were analysed in whole serum as well as immunoglobulin fractions and purified RF from patients with one of three autoimmune disorders. As predicted from the pI ranges of normal human serum IgM and IgA in agarose, IgM RF had near neutral pIs, whereas IgA RF showed more acidic (lower) pI values. Serum IgA and IgM RF from patients with rheumatoid arthritis or the sicca syndrome showed considerable charge heterogeneity whereas IgM RF from mixed cryoglobulinaemia or rheumatoid vasculitis patients showed monoclonal and oligoclonal banding patterns respectively. Clonotypic analysis was best achieved with isolated light and heavy chains from highly purified RF. The IgA RF spectrotypes from unmodified paired serum and saliva IgA were clearly different whereas after desialation, similar pI values and, in one case, similar spectrotypes were observed. These observations are compatible with the hypothesis that serum and saliva IgA RF are derived from similar clonal precursors. The methods used in these studies may also be of use in the analysis of IgA and IgM antibody diversity in a number of other situations. | |
| 2075369 | Two-dimensional electrophoresis of synovial tissue, synovial fluid and serum in patients w | 1990 | Using the technique of two-dimensional (2D) electrophoresis with consecutive silver staining, we investigated samples of serum, synovial fluid and synovial tissue obtained from 19 patients suffering from rheumatoid arthritis (RA) or non-RA arthritis. From these experiments we have drawn the following conclusions. 2D electrophoresis of serum, synovial fluid and synovial tissue extracts taken from patients suffering from joint diseases is a reproducible method. Repeated runs of the same sample reveal an essentially constant protein spot pattern. The time period between surgery and tissue preparation did not influence the number of protein spots when less than 15 h was involved. The protein spot number is always lower in synovial fluid than in either synovial tissue or serum in RA and non-RA patients. The mean value for the number of spots is 68 for the inflamed tissue irrespective of the cause of arthritis (RA and non-RA group taken together) and 47 for the control group. This difference is significant. We were able to definitely identify 7 spots in the tissue extract. We did not find RA-specific protein spots in either serum, synovial fluid or tissue extracts from the synovial membrane. The only significant difference between RA patients and either non-RA or control group patients concerning the protein spot pattern is the increased size of the immunoglobulin spot (mainly IgG) in RA. In addition, we discuss possible reasons for failure of the 2D electrophoresis technique to detect disease-specific protein patterns. | |
| 1917191 | Longterm analysis of Sheehan total knee arthroplasty. | 1991 | Sheehan total knee arthroplasty was carried out in 405 knees at two centres. The patients were followed up from two to eleven years. The clinical results were assessed, the complications documented and the influence of specific factors on the result was evaluated. The principal complications arose from the patella and the femorotibial articulation. The low rate of loosening and the achievement of satisfactory alignment lead the authors to support the concept of a long stem. | |
| 2380520 | Convex condylar arthroplasty of the basal joint of the thumb: failure under load. | 1990 Jul | Twenty-five convex condylar silicone arthroplasties were done for treatment of arthritis confined to the basal joint of the thumb. The procedure was done in 19 patients with osteoarthritis and in 6 patients with rheumatoid arthritis. The average follow up was 4 1/2 years. This series of condylar arthroplasties is compared with a series of 24 Swanson trapezial arthroplasties, all done for treatment of osteoarthritis, with an average follow up of 5 1/2 years. The convex condylar arthroplasty is associated with a 15% failure rate in patients with osteoarthritis coupled with radiographic findings of significant bone resorption around the stem of the prosthesis in 84% of the remaining patients. The three failures were in the osteoarthritic thumbs and not in the rheumatoid thumbs. Fusion and tendon interposition arthroplasty are described as options for revision. We believe that the Swanson convex condylar hemitrapezium replacement should be reserved for the low-demand rheumatoid thumb. | |
| 3572409 | Infected total knee arthroplasty. A protocol for management. | 1987 | All cases of deep wound infection following total knee arthroplasty presented to the senior author between April 1977 and February 1984 were reviewed in an attempt to develop a protocol for salvaging a functional, painless knee. Two of 23 knees were eliminated because of extensive soft tissue loss. The remaining 21 infected total knee arthroplasties were analyzed. A determination of the chronicity of the infection and an evaluation of any radiolucencies were essential in determining the type of treatment used. Three distinct methods of surgical management were identified: radical debridement with retention of the initial prosthesis, one-stage reimplantation, and two-stage reimplantation. A functional uninfected total knee arthroplasty was salvaged in 18 of 21 patients, with an average follow-up period of 46 months. | |
| 2429246 | [The knee joint]. | 1986 Aug | The knee is very important in the treatment of rheumatic diseases. Differentiating between the indications for different forms of therapy is dependent upon knowledge of the prospects for success. Synovectomy, preventive and palliative procedure, is the most important joint-saving measure, as confirmed by the long-term results presented here. Synoviorthese and arthroscopic operative procedures are alternatives and have expanded the spectrum. However, their long-term effectiveness has not yet been proved. Rheumatic, recurring synovitis has not yet been defined satisfactorily. Additional soft tissue operations should always be included in preoperative planning and determine the form of therapy. Alloarthroplasty of the knee is the foremost reconstructive measure, and excellent long-term results have already been achieved in some cases. It should be remembered, however, that modifications of individual models occasionally have a considerable influence on the results. Particularly for rheumatic knee joint deterioration, knee prosthesis models that guarantee long-term success should be given preference if the time and effort expended are justifiable. Continuous passive mobilization seems to be the most valuable postoperative measure. | |
| 1978706 | [Preliminary study on the cellular immune abnormality in rheumatoid arthritis]. | 1990 May | The cellular immunological abnormalities in 21 patients with rheumatoid arthritis (RA) were studied with flow cytometer. In the peripheral blood monocytes (PBM) from patients with active RA the number of HLA-DR positive cells were remarkably increased. The activity of NK cells and Leull C+ cells reduced to 1/2-1/3 of the normal limits. The AMLR and mitogen-inducing reaction decreased significantly. These data suggest that the changes of cellular immune reaction and immunoregulation may be related to the pathogensis of RA. | |
| 3442969 | Juxta-articular adiposis dolorosa associated with rheumatoid arthritis. Report of 2 cases | 1987 Sep | Juxta-articular adiposis dolorosa, i.e., painful fatty deposits around the joint may occur in postmenopausal obese women. The association of the syndrome with osteoarthritis has been described. The present report describes 2 women suffering from rheumatoid arthritis with painful fatty deposits around the knees. Both responded to local injection of corticosteroid. The possible pathogenesis of the source of pain is discussed. | |
| 3789478 | [Autoimmunity to collagen, experimental arthritis and rheumatic disease]. | 1986 | Auto-immunity to type II collagen is now recognised as a good experimental model of arthritis. The possibility of a post-immunization treatment with collagen to suppress or abrogate the arthritic response is particularly interesting in the therapeutical trends suggested by such a possibility. In human pathology, if the pathogenic role of anti-native collagen type II auto-antibodies is well demonstrated in relapsing polychondritis, in the contrary, this hypothesis does not seem relevant to rheumatoid arthritis. In this disease, the role of the auto-immunity to native collagen appears secondary. | |
| 2380229 | The Roper-Day total shoulder replacement. | 1990 Jul | A simple unconstrained shoulder prosthesis has been used in 22 patients (25 shoulders) with incapacitating pain and severely damaged joints who, because of age or generalised rheumatoid disease, have limited functional requirements. All reported good and lasting pain relief. Improvement in range of movement was modest, but there was marked improvement in function. In this group of patients, rotator cuff damage is common, but does not preclude a satisfactory result. | |
| 3389926 | Identification by cell electrophoresis of a subpopulation of polymorphonuclear cells which | 1988 May | Two and occasionally three electrophoretic subpopulations of polymorphonuclear cells (PMNs) were identified in the blood of normal healthy subjects and patients with rheumatoid arthritis (RA). Most of the PMNs from both groups of subjects were found in the population with the highest surface charge; the remainder being in the other distributions, which were collectively termed the slow cell population. There was a significant increase in the percentage of rheumatoid PMNs (mean 42%) ascribed to the slow population when compared with PMNs from normal subjects (mean 17%). A similar increase in the slow cell population was also seen in patients with Felty's syndrome (mean 38%) and scleroderma (mean 51%), but not in patients with Behçet's syndrome (mean 18%). Synovial fluid aspirated from the knee joints of patients with RA contained PMNs with the lowest surface charge. With nylon fibre as an adherence substrate cells of a low surface charge were found to be more adherent than those of a high surface charge. An alteration in the electrophoretic distribution of PMNs may represent changes that are related to the expression of functionally related membrane ionogenic groups. | |
| 3286079 | False aneurysm of the common femoral artery secondary to migration of a threaded acetabula | 1988 Jun | A 66-year-old woman with severe rheumatoid arthritis had revision of a protrusio acetabuli component with a threaded socket. Subsequent anteromedial migration of the socket and thread laceration of the common femoral artery resulted in a pseudoaneurysm and massive pelvic erosion seven months later. Vascular reconstruction and excisional arthroplasty were necessary. Later, gracilis and gluteal muscle flaps were swung to fill the resultant pelvic cavity. The hip was nonreconstructable. Ten months later the wound healed and the patient was ambulating in a walker. This represents a previously unreported cause of vascular injury complicating total hip arthroplasty (THA). A comprehensive review of the English and European literature illustrates the various mechanisms of vascular trauma in this setting and provides a rational basis for prevention. | |
| 3783180 | Mood changes associated with "end-of-dose deterioration" in Parkinson's disease: a control | 1986 Oct | Fluctuations of mood and psychic activation were assessed in relation to "mobile" and "immobile" periods in 18 patients with Parkinson's disease presenting typical "end-of-dose deterioration". Twelve subjects with chronic but active rheumatoid arthritis presenting increased physical disability due to severe morning joint stiffness with a repetitive pattern of mobile and immobile periods acted as controls. The overall degree of disability and its fluctuations were close enough in the two groups for fair comparison of changes in affective behaviour. Temporary immobility was accompanied by adverse changes of mood and activation, which were significantly more marked in the Parkinson's disease group than in the controls, though to a lesser extent in those Parkinson's disease patients rated depressed even in their mobile periods. The possible correlation between mood changes and fluctuations of central dopaminergic function in the Parkinson's disease patients is discussed. | |
| 3485430 | Collagen antibodies in rheumatoid arthritis. Significance of antibodies to denatured colla | 1986 Feb | The frequency, specificity, and HLA associations of antibodies to collagen were examined in 54 patients with severe rheumatoid arthritis (RA) and in 67 control subjects, using native and denatured bovine type II collagen as reactants in a solid-phase radioimmunoassay. Reactivity to denatured collagen was significantly higher in the RA patients than in the controls (P = 0.004). Reactivity to native collagen was substantially lower than reactivity to denatured collagen and was similar in RA patients and controls. DR4 positive RA patients had significantly greater reactivity to denatured collagen compared with DR4 negative RA patients (P = 0.03), but levels of antibody to native collagen were similar among DR4 positive and DR4 negative patients. These data lend support to the idea that denatured collagen is an important secondary reactant in immune-mediated perpetuation of RA. |