Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 2648744 | Immune-inflammatory response in infected arthroplasties. | 1989 Feb | We studied the immunocytology of synovial fluid in purulent endoprosthetic infections using cell subtype-specific monoclonal antibodies in avidin-biotin-peroxidase complex staining. Two thirds of the monocytes were CD15-positive, whereas CD2-positive T lymphocytes only formed one third of all the mononuclear cells. The synovial fluid monocyte-activated T-cell ratio differed from findings in sterile inflammatory, reactive and rheumatoid arthritis. | |
| 1712867 | [Rapid assay of hyaluronic acid in serum]. | 1991 May | A method for measurement of hyaluronic acid (HA) level in serum was developed based on using "hyaluronic acid binding protein" (HABP)-coated polystyrene beads. After the beads and test serum being mixed, the mixture was incubated together with reaction buffer for 2 hours, and then the beads were washed. Subsequently, biotinylated HABP was added to the washed beads and incubated for 1 hour. Then peroxidase-conjugated avidin was added to the mixture and incubated again for 1 hour. After the beads being washed, a substrate solution was added to the washed beads and left for 1 hour. Then the reaction was stopped by adding 2N-H2SO4. The absorbance at 492 nm was recorded. The analytical range of HA in serum by this method was found to be between 10-800 micrograms/l, and the precision of the HA assay (CV%) was between 3.0-8.4 in the "with-in" assay (n = 10), and 4.8-8.9 in the "between" assay (n = 5). The analytical recovery of HA assay was between 92-115%. In this study, the results in screening of the serum HA level in RA patients (n = 107), OA patients (n = 16) and healthy subjects (n = 30) showed that the HA level of RA patients was demonstrated significantly higher than that of healthy subjects and OA patients. | |
| 2592387 | The fate of acetabular allografts after bipolar revision arthroplasty of the hip. A radiog | 1989 Dec | We reviewed the radiographs of thirty-one patients (thirty-two hips) who had had revision of the acetabular component of a total hip arthroplasty with a bipolar socket supplemented by allograft and were followed for twenty-four to forty-eight months. The grafts were categorized according to their consistency (solid or crushed bone), the location of the acetabular defect (peripheral [rim] or central), and the extent of the acetabular defect (contained--the medial part of the acetabular wall was intact, or non-contained--it was deficient). We recorded the time to incorporation of the graft, the amount of migration of the socket in the superior and medial axes, and the percentage of graft remaining at the time of the most recent follow-up. The time to healing was similar for all categories of grafts. The central, contained, solid grafts had less resorption than did the central, contained, crushed-bone grafts, as evidenced by less migration of the socket during follow-up. The non-contained grafts, in both peripheral and central locations, were associated with high rates of migration and of instability of the socket. | |
| 2359072 | High levels of antipeptidoglycan antibodies in psoriatic and other seronegative arthritide | 1990 May | Bacterial cell wall peptidoglycan, the arthritogenic factor in adjuvant induced arthritis, may also be involved in the etiology of some human rheumatic diseases. Patients with some seronegative rheumatic diseases like ankylosing spondylitis and Reiter's syndrome have elevated antibody titers to peptidoglycan. Using an ELISA with soluble peptidoglycan, we examined the sera of 110 patients with psoriatic arthritis, psoriasis without arthritis and a variety of other joint diseases. Antibody titers were significantly higher (p less than 0.001) among the 22 patients with psoriatic arthritis than the 16 patients with psoriasis without arthritis. Patients with other seronegative arthritides also had higher levels of antipeptidoglycan antibodies than patients with rheumatoid (seropositive) arthritis, osteoarthritis and crystal induced arthritis. Our results furnish additional support for the suggestion for a bacterial role in the pathogenesis of psoriatic and some other seronegative arthritides. | |
| 3626164 | A case of acute interstitial nephritis induced by flurbiprofen. | 1987 May | Recently, acute interstitial nephritis (AIN) presenting nephrotic syndrome and renal failure induced by nonsteroidal anti-inflammatory drug (NSAID) has been recognized with increasing frequency. We described here a 43-year-old woman who developed this type of nephropathy after taking NSAID for rheumatoid arthritis. Flurbiprofen (Froben) was assumed to be a causal drug based on a clinical course and a positive result of lymphocyte transformation test. Withdrawal of flurbiprofen therapy led no sufficient improvement, and high-dose steroid therapy done 15 months after the onset resulted in only a minor improvement. So far as we know, this was the second case of AIN associated with flurbiprofen and the youngest in NSAID-induced AIN with irreversible chronic renal insufficiency. | |
| 3581567 | Postoperative patellar complications with or without lateral release during total knee art | 1987 Jun | Between 1975 and 1984, 84 knees in which an intraoperative lateral release had been performed with total knee arthroplasty (TKA) were compared with 471 knees that, having sufficient patellar tracking at the time of surgery, had not required a lateral release. The lateral superior geniculate artery was routinely sacrificed in all knees in which a lateral release was necessary. The results showed no complications associated with a possible loss of blood supply secondary to the lateral release. No increased evidence of osteonecrosis, patellar bone-cement radiolucency, or patellar fracture was discovered. Clinically, there was no difference in pain, ambulation, or range of motion. Seventeen patellar fractures (3.6%) were observed in patients for whom a lateral release had not been performed, yet only one patellar fracture (1.5%) was encountered among the patients who had required a lateral release. In TKA, lateral release was associated with a relatively high degree of patellar tilting. | |
| 2957787 | Risk factors that may influence development of side effects of gold sodium thiomalate. | 1987 | Two hundred and seventy-seven patients who had received gold sodium thiomalate (GST), were included in a retrospective study to identify those factors that might have influenced the development of side effects. Fifty-one per cent of patients were excluded due to side effects, especially skin rash, which affected 59% of those excluded. The duration of disease when GST therapy was commenced, female sex, and a history of smoking were identified as factors influencing the development of certain side effects. There was a high incidence of skin rash in smokers. Of 99 smokers, 40% developed dermatitis, whereas in 109 non-smokers, only 17% developed a rash (p less than 0.001). Other factors such as age, weight, and concurrent drug therapy, biochemical, haematological and immunological data, do not predict development of toxicity to GST. | |
| 3238942 | [Pentose phosphate pathway of carbohydrate metabolism in various systemic diseases of the | 1988 Nov | Ratios of activities of oxidative and nonoxidative enzymes involved in the pentose phosphate pathway of carbohydrate metabolism were altered in blood plasma and cells of the patients with systemic impairments of connective tissue. In rheumatoid arthritis and systemic lupus erythematosus the enzymatic activity was increased in blood plasma and cells, while the most distinct activation of the enzymes was found in granulocytes. In systemic sclerodermia total activity of the enzymes involved in metabolism of pentose phosphates in granulocytes exceeded 2.6-fold their values under conditions of normal state, whereas activities of the other enzymes studied remained near normal values. Calculation of ratios (mu value) between activities of the pathway oxidative and nonoxidative enzymes showed that the mu value was increased 2.7-fold in rheumatoid arthritis, while this value was decreased in lupus erythematosus and systemic sclerodermia 4.2- and 2-fold, respectively. The mu values, calculated on the basis of estimation of total activity of dehydrogenases from pentose phosphate pathway and total pentose phosphate metabolizing activity, might serve as a convenient diagnostic criterion for estimation of the ratio between activities of oxidative and nonoxidative enzymes involved in pentose phosphate pathway in granulocytes used for differential diagnosis of systemic impairments of connective tissue (rheumatoid arthritis, lupus erythematosus and systemic sclerodermia). | |
| 2148640 | T gamma delta cells and their subsets in blood and synovial tissue from rheumatoid arthrit | 1990 Dec | We have examined the frequencies of T gamma delta cells in blood, synovial fluids, and synovial membranes of patients with rheumatoid arthritis (RA) and in blood from age-matched controls. Immunocytochemical and immunohistochemical techniques were used with monoclonal antibodies BB3 and A13 to define a major and minor blood subset of T gamma delta cells respectively. Together, these antibodies identify the majority (if not all) of the peripheral blood T gamma delta cells. Significantly lower levels of T gamma delta cells were found in the blood of RA patients compared with controls, whilst higher but not significant numbers were found in the synovial fluids of paired samples. Scattered T gamma delta cells were found only in some synovial membranes with a distribution similar to the T alpha beta cells. Analysis of the two different T gamma delta-cell subsets indicated a ratio of BB3 to A13 of about 5:1 in control and RA blood. However, this ratio was less than 1:1 in the RA synovial fluids and membranes. The migratory nature of the A13+ cells could account for their predominance in these sites. The possible pathological significance of these cells in the rheumatoid synovial fluid and synovial membranes is discussed. | |
| 3368786 | A molecular basis for MHC class II--associated autoimmunity. | 1988 May 20 | Class II major histocompatibility (MHC) molecules have an immunoregulatory role. These cell-surface glycoproteins present fragments of protein antigens (or peptides) to thymus-derived lymphocytes (T cells). Nucleotide sequence polymorphism in the genes that encode the class II MHC products determines the specificity of the immune response and is correlated with the development of autoimmune diseases. This study identifies certain class II polymorphic amino acid residues that are strongly associated with susceptibility to insulin-dependent diabetes mellitus, rheumatoid arthritis, and pemphigus vulgaris. These findings implicate particular class II MHC isotypes in susceptibility to each disease and suggest new prophylactic and therapeutic strategies. | |
| 1708263 | T-cell epitopes on the 70-kDa protein of the (U1)RNP complex in autoimmune rheumatologic d | 1990 Dec | High-titre IgG antibodies against the immunodominant 70-kDa protein of the (U1)ribonucleoprotein (RNP) complex are present in virtually 100% of patients with mixed connective tissue disease (MCTD), and less commonly in a variety of other autoimmune rheumatic diseases. As T-cell 'help' is assumed to be required for this potentially pathogenic form of immune response, investigations to define T-cell epitopes on the 70-kDa protein were undertaken. In prior studies we expressed the 70-kDa protein and a number of its fragments, spanning most of the molecule, as recombinant fusion proteins using the pGEX expression-vector system. These fusion proteins were used as antigens in the epitope mapping studies reported here. PBMC were isolated from patients with (U1)RNP-positive rheumatic diseases and from both normal controls and rheumatologic patients with other autoantibody reactivities, including those to Ro, La and dsDNA. Reactivity to the purified 70-kDa protein was assayed by thymidine incorporation and was evident only in anti-(U1)RNP positive patients but was not restricted to MCTD patients, being present also in patients with SLE and rheumatoid arthritis. The stimulation indices (SIs) observed were in the two- to five-fold range. Using the 70-kDa protein fragments, a T-cell stimulatory epitope was localized to the C-terminal 63 amino acids of the autoantigen. A T-cell line, derived from PBMC of a (U1)RNP positive patient with MCTD, also reacted predominantly with this C-terminal fragment but with an SI of approximately 15-fold. Thus, we have demonstrated the presence and specificity of autoreactive T lymphocytes to a defined peptide epitope in systemic rheumatic disease. | |
| 2775319 | Secretion of antibodies to types I and II collagen by synovial tissue cells in patients wi | 1989 Sep | Production of antibodies to IgG and to type I and type II collagen (CI and CII) was analyzed by enzyme-linked immunospot assay in patients with rheumatoid arthritis (RA) and patients with other inflammatory or degenerative joint diseases. Anti-CII-secreting cells, generally in high numbers, were found among mononuclear cells eluted from inflamed synovial tissue in 12 of 13 patients with seropositive RA and 9 of 14 patients with seronegative RA or with undetermined serum rheumatoid factor levels. In contrast, no anti-CII-producing B cells were present among synoviocytes from 4 patients with other joint diseases. In none of 7 RA sera did we find significant levels of anti-CII. Synovial B cells secreting antibodies specific for CI were observed less frequently in patients with RA. These results indicate that measurement of serum antibody levels is not adequate to assess actual autoantibody production in rheumatoid joints and that local autoimmune reactions to CII are common in RA, which implies that collagen-reactive T cells are present within the inflamed joints of RA patients. The possible role of a local collagen autoimmunity in RA is discussed, particularly in relation to its putative role in rheumatoid factor production. | |
| 2101241 | [Posterior osteosynthesis in C1-C2 instability]. | 1990 | This is a retrospective study of 8 cases of instability of the C1-C2 segment caused by R.A. or non-union and treated by posterior stabilization with wire loops and bone grafts. The authors reported good results in patients with mild recent neurological involvement and/or radiographic instability of the segment (posterior subluxation of the dens greater than 1/3 of the A-P diameter of C1 or tilting of the dens greater than 30 degrees). The death of a patient with non-union of the dens and severe neurological involvement due to respiratory palsy leads the authors to regard severe long-term neurological involvement (quadriparesis) a contraindication for this type of procedure. | |
| 2956956 | T cytotoxic and helper cells are markedly increased, and T suppressor and inducer cells ar | 1987 Jul | Synovial fluid lymphocytes and paired peripheral blood lymphocytes from 28 patients with rheumatoid arthritis were analyzed by 2-dimensional flow cytometry. We found that the composition of the lymphocyte population is different in synovial fluid compared with that in peripheral blood, in that there were increased proportions of Ia+ Leu-2a+/Leu-15- (T cytotoxic) cells and Leu-3a+/Leu-8- (T helper) cells, together with marked decreases in Leu-2a+/Leu-15+ (T suppressor) cells and Leu-3a+/Leu-8+ (T inducer) cells. These findings suggest that the unique composition of synovial fluid lymphocytes might be the result of T cell activation by some mechanism that is not known. | |
| 3745240 | The influence of tibial-patellofemoral location on function of the knee in patients with t | 1986 Sep | Function of the knee and patellofemoral symptoms were correlated with the position of the implant in 101 consecutive patients with 116 posterior stabilized condylar knee prostheses. All of the patients were followed for a minimum of two and a half years with sequential physical examinations, radiographs, and functional evaluation of the knee. In sixteen knees (14 per cent), clicking or catching of the patella in terminal extension or painless crepitation throughout the arc of flexion developed without lowering the functional knee-evaluation score. Pain or mechanical problems, or both, that lowered the functional knee-evaluation score occurred in another fourteen knees (12 per cent), within the first postoperative year. Of these fourteen, eight required revision solely for patellofemoral complaints. Critical analysis of the tibial-patellofemoral mechanical axis identified three surgical variables that were found to markedly affect the functional result of the prosthesis: the distance from the center line of the tibial prosthesis to the center line of the tibial plateau, a change in the position of the joint line of the prosthesis relative to the hip and ankle, and the patellar height, measured as the perpendicular distance from the inferior pole of the patellar implant to the joint line of the prosthesis. Functional knee scores, range of motion, patellofemoral pain or mechanical symptoms, the need for revision, and the necessity of manipulation could all be statistically significantly correlated with the three independent variables. In addition, a range of neutral alignment was developed.(ABSTRACT TRUNCATED AT 250 WORDS) | |
| 2333557 | Pigmented villonodular synovitis of the knee: computed tomographic appearances. | 1990 | Five patients with pigmented villonodular synovitis (PVNS) of the knee demonstrate the value of preoperative, contrast medium-enhanced CT scanning. The technique is shown to be particularly useful in locating recurrent lesions and in demonstrating that popliteal and posterior calf masses are due to PVNS affecting the knee joint itself. In two of the five patients PVNS was not considered a possibility before the CT scan, and none had evidence of bone erosion on plain radiographs. In none of the patients was the unenhanced CT scan able to demonstrate evidence of decreased radiolucency due to fat within the tumour or density greater than the adjacent skeletal muscle, indicating haemosiderin deposits. | |
| 2786784 | Constitutive production of interleukin 6/B cell stimulatory factor-2 from inflammatory syn | 1989 Aug | Interleukin 6 (IL-6) production from synovial tissues of various diseases was examined. Augmented IL-6 production was found in inflammatory synovium not only in RA but also in other kinds of synovitis, including psoriatic arthritis and Behçet's disease. Increased amounts of IL-6-mRNA were detected in rheumatoid synovium using a dot blot hydridization technique. Furthermore, there was a positive correlation between IL-6 production and accumulation of plasma cells in the synovium. These findings suggest that IL-6 plays an important role not only in immune response but also in active inflammation in various kinds of synovitis. | |
| 3055745 | [Can one increase the analgesic effect of Mobilisin ointment by iontophoresis? Placebo con | 1988 Jul | In a double-blind, three-way crossover trial, with placebo controls on 41 patients with inflammatory rheumatic disorders (mono- or oligoarticular forms), the analgesic action of Mobilisin ointment, with or without iontophoresis, was significantly stronger than after iontophoresis alone or after placebo. Iontophoresis did not potentiate the analgesic properties of Mobilisin. The pain was scored on VAS in intervals 30 min, 1, 2, 4, 6, 8 h after application. The duration of analgesic effect after Mobilisin procedures was 6.5 h on average, which was significantly longer than after iontophoresis alone or after placebo. | |
| 2023212 | Longterm treatment with nonsteroidal antiinflammatory drugs in rheumatoid arthritis: a pro | 1991 Feb | The longterm use of nonsteroidal antiinflammatory drugs (NSAID) was studied in a consecutive sample of 148 patients with recently diagnosed rheumatoid arthritis. A survival analysis was done in which treatment terminations due to side effects and to insufficient therapeutic effect were used as index causes. Cumulative drug 'survival' of indomethacin with treatment terminations due to untoward reactions as endpoints was significantly less compared to naproxen and diclofenac (p less than 0.001). No significant difference with termination due to lack of efficacy as index cause was observed (p greater than 0.90). No influence of sex or age on the drug survival with the different endpoints mentioned above could be established. | |
| 2662370 | [The HLA system in rheumatic practice: from structure to typing]. | 1989 Apr 30 | The HLA system regulates specific immune responses by presenting a processed foreign antigen (likely a peptide) to T lymphocytes. The peptide is associated with an HLA class I molecule (HLA A, B, C) when CD8 + T lymphocytes are activated to become cytotoxic. Alternatively when a peptide is associated with an HLA class II (HLA DR, DQ, DP) molecule CD4 + T cells are activated (helper T cells) which in turn stimulate B cells to produce specific antibodies. Recent studies have unravelled the three-dimensional structure of one HLA class I molecule and provided a framework for the 3D structure of class II molecules. This allow an understanding of the complex structure-function relationship of the HLA molecule with the antigen and the T cell receptors. Apart from transplantation the importance of the HLA system in medicine is due to its central role in auto-immunity. The HLA system is involved both at the genetic level (various disease susceptibilities are associated with particular alleles of HLA class I or class II) and at the phenotypic level (hyperexpression of HLA class I molecules and aberrant expression of class II molecules are observed on diseased tissues). Recently several molecular techniques have been developed for HLA typing including isoelectrofocalisation (IEF), two dimensional gel electrophoresis (2D PAGE) RFLP or oligonucleotide probing (ASO). They provide an accurate and precise way of typing and thus are very valuable in studying HLA and disease associations by defining the molecules and epitopes responsible for high genetic susceptibility or resistance to the relevant disease. |