Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 2671367 | Antinuclear antibody in juvenile rheumatoid arthritis sera reacts with 50-40 kDa antigen(s | 1989 Jul | To identify whether sera containing antinuclear antibody (ANA) from patients with juvenile rheumatoid arthritis (JRA) recognize a common antigen, extracts of HeLa nuclei were prepared by incubating the nuclei with buffers of sequentially increasing salt concentrations (0.1 M, 0.3 M, and 0.5 M NaCl). Western blot analysis using sera from 82 patients with JRA revealed that 63% of ANA positive JRA sera reacted with 2 bands (50-40 kDa) in the 0.3 M extract whereas only 14% of ANA negative JRA sera had antibody to these bands. Antibody to the 50-40 kDa antigen(s) strongly correlated with the presence of ANA by immunofluorescence and was found in 70% of patients with iritis; however, it did not correlate with disease subgroup or disease activity. | |
| 2671366 | Abnormalities of immunoregulation in juvenile rheumatoid arthritis. | 1989 Jul | Immunoregulatory imbalances are thought to be involved in the pathogenesis of juvenile rheumatoid arthritis (JRA). We have found that a subset of patients with JRA demonstrate a marked expansion of B cells without an alteration in B cell subset distribution. However, there was actually decreased in vitro immunoglobulin production in response to stimulation with either pokeweed mitogen or hydrocortisone. These B cell abnormalities were found to correlate with a marked increase in the percentage of CD4 + CD45R + T cells, a T cell subset thought to be responsible for inducing suppression. In addition, there was a significant decrease in the percentage of CD4 + CD29 + T cells, a T cell subset thought to be responsible for inducing B cell immunoglobulin production. Our results suggest that the B cell abnormalities seen in JRA may be related to defects in T cell immunoregulation. | |
| 2213782 | Clonal analysis of joint fluid T lymphocytes in patients with juvenile rheumatoid arthriti | 1990 Aug | Synovial fluid (SF) lymphocytes from 4 patients with pauciarticular juvenile rheumatoid arthritis (JRA) and 4 patients with polyarticular JRA were examined for their phenotypic and functional characteristics. In all 8 patients there was a high proportion of activated SF T cells, together with an increased proportion of CD2+CD3- and the presence of CD3+CD4-CD8-WT31- lymphocytes. The functional analysis at the clonal level in 5 patients (427 clones) showed a relevant proportion of cytotoxic T cell clones, which were not confined to typically cytolytic phenotypes, but were also present among CD3+CD4+CD8- cultures. Compared to those with pauciarticular JRA, patients with polyarticular disease had a significantly higher proportion of T cell clones with cytotoxic activity. Although derived from a limited number of patients, our data suggest a direct involvement of T cells in the pathogenetic mechanisms that originate and maintain the articular damage, and the possibility of different or more pronounced T cell reactivities in the clinically more diffuse JRA types. | |
| 2326600 | Mandibular condyle lesions, jaw movements, and occlusal status in 15-year-old children wit | 1990 Feb | The purpose was to determine the number and severity of lesions in the temporomandibular area and their association with occlusal status and mandibular movements in 15-yr-old children afflicted with juvenile rheumatoid arthritis (JRA). The series comprised 121 JRA children, 78 girls and 43 boys, and a control group of 104 schoolchildren, 70 girls and 34 boys, taken for clinical examination. Of the JRA children, 55% had roentgenologically detectable lesions in the TMJ area. Lesions were found in 60% of the girls and in 42% of the boys, the ratio thus being 3:2. The largest group had grave lesions (48%), the second largest displayed flattening of the condyle (43%) and the smallest a flattened condyle with a minor lesion (9%). There were more bilateral lesions in the children with the severest lesions. Tendencies for Class II malocclusion, small vertical overbite and open bite were visible in the JRA children. Reduced maximal opening capacity, maximal protrusion, lateral movements of the mandible and pain in the TMJ area proved to be reliable signs predicting temporomandibular joint involvement in JRA children. Maximal mouth opening capacity was significantly restricted in the patients showing condylar lesions as compared with patients having no lesions or with controls. JRA children should be divided according to sex and the severity of the lesions. The JRA children without TMJ lesions had normal values for mandibular movements and were thus comparable to healthy children. | |
| 2664990 | [Computer-assisted evaluation of the symptoms of juvenile rheumatoid arthritis]. | 1989 Jan | Data on 371 patients with juvenile RA were processed on a special computer program. Special informative signs were determined for various forms of juvenile RA have been established. Efficacy of computer processing for the diagnosis of RA has been stressed. | |
| 3772655 | Childhood Lyme arthritis: experience in an endemic area. | 1986 Nov | We report 25 children with oligoarticular arthritis associated with Lyme disease. There were 16 boys (male/female ratio 1.8:1); ages ranged from 2 to 15 years. Thirteen (52%) children had no history of erythema chronicum migrans or other rash. Thirteen had temperatures as high as 41 degrees C for up to 2 months before the onset of arthritis. Twelve recalled definite tick bites. Ten (40%) children, of whom seven had no history of rash, were hospitalized for presumed septic arthritis. Another four had diagnoses of pauciarticular juvenile rheumatoid arthritis for as long as 3 years. Seven patients had less acute, recurrent episodes of synovitis. Two children had seventh nerve palsies 2 months before onset of arthritis. All patients had antibodies to the Lyme spirochete. In 14 patients, synovial fluid white blood cell counts ranged from 180 to 97,700/mm3 (greater than or equal to 76% polymorphonuclear leukocytes). Antibiotic therapy was effective in all patients; in 13, orally administered therapy alone resulted in elimination of synovitis and recurrent attacks. Lyme arthritis may be confused with acute bacterial septic arthritis or recurrent "pauciarticular juvenile rheumatoid arthritis," particularly when there is no history of erythema chronicum migrans. | |
| 2188388 | Juvenile arthritis and uveitis. | 1990 Jan | The association between juvenile arthritis and uveitis is reviewed. Some children with the HLA-B27 related spondyloarthropathies develop anterior uveitis. About 20% of patients with juvenile rheumatoid arthritis (JRA) who are negative for IgM rheumatoid factor develop a frequently bilateral, nongranulomatous chronic anterior uveitis. Risk factors for uveitis in JRA patients are: female gender, pauciarticular onset of arthritis, presence of circulating antinuclear antibodies, and the antigens HLA-DW5 and HLA-DPw2. Uveitis is rare after seven years or more have elapsed from the onset of arthritis. The visual prognosis in patients with uveitis is good in 25% and fair in 50%. The remaining 25% develop visual impairment from complicated cataract and/or secondary inflammatory glaucoma. The potential benefit of cytotoxic agents in the treatment of intractable uveitis is outweighed by the risk of serious side effects. The management of secondary inflammatory glaucoma is unsatisfactory, but the results of treatment of complicated cataracts by lensectomy-vitrectomy are good. | |
| 1790646 | Adult-onset Still's disease associated to toxoplasma gondii infection. | 1991 Sep | Adult-onset Still's disease is characterized by high spiking fever, evanescent maculopapular rash and arthritis. Several recent reports have associated its development with a variety of infectious agents. We describe the case of a 25-year old woman presenting high fever, maculopapular rash and seronegative polyarthritis associated with lymphoadenopathy, splenomegaly and neutrophil leucocytosis together with acute acquired toxoplasmosis. Other causes of systemic illness were excluded by appropriate laboratory, radiological and histological investigations. Clinical, radiological and laboratory findings as well as possible etiopathogenetic correlations among both pathological conditions are discussed. Toxoplasma gondii should be considered as a further possible triggering agent associated with the development of adult-onset Still's disease. | |
| 1920313 | Treatment of fulminant adult Still's disease with intravenous pulse methylprednisolone the | 1991 Jul | We describe a patient with fulminant adult onset Still's disease and multiple organ involvement. The acute flare persisted despite high doses of prednisone but responded to megadose intravenous pulse methylprednisolone therapy. This treatment modality may be useful in temporizing severe multisystem exacerbations of adult Still's disease. | |
| 2661570 | Psoriatic arthritis in the hand. | 1989 May | Psoriatic arthritis is a polyarthropathy distinct from rheumatoid arthritis and associated with onychodystrophy and skin lesions. Patients present with stiffness, flexion and extension contractures, digital swelling, or painful joints. Psoriatic arthritis mutilans is a rapidly progressive osteolysis resulting in severe deformity. Radiographs reveal widened joint spaces with interphalangeal joint destruction, resorption of the distal tufts, and frequently spontaneous fusion. Surgery involves mainly salvage procedures to relieve pain or improve position, as the increase in motion obtained usually is limited. | |
| 27319879 | Arthritis - joint action. | 1987 Nov 14 | Last month the Horder Centre for Arthritics in Crowborough, Sussex celebrated its coming of age. A charitable organisation founded 21 years ago by Miss Cecilia Bochenck, a sufferer from rheumatoid arthritis from the age of six, the Centre provides short and long term inpatient care as well as a service to outpatients. | |
| 2975541 | IL-2 production in the autologous mixed lymphocyte reaction of patients with juvenile rheu | 1988 Oct | Interleukin 2 (IL-2) production in the CD4/non-T cell autologous mixed lymphocyte reaction (AMLR) was estimated for patients with juvenile rheumatoid arthritis (JRA). A cellular IL-2 assay (CILA) consisting of co-cultures of indicator cells, CTLL-2, added to CD4/non-T cell AMLR cultures was used. As both CD4 and CTLL-2 cell proliferation are IL-2 dependent, results of the CILA assay were taken as a reflection of the total IL-2 in the co-culture. By this assay most patients with JRA had normal IL-2 production. However, five patients with polyarticular disease had both a low AMLR and low levels of IL-2 production. Three of those tested showed little improvement in the AMLR on addition of recombinant IL-2, suggesting decreased responsiveness to IL-2 as well. | |
| 2236686 | [Multicentric reticulohistiocytosis (lipoid dermato-arthritis). A radiologic study of 3 ca | 1990 Sep | The authors report their experience with 3 cases of multicentric reticulohistiocytosis observed over 6 years of outpatient radiological practice. The condition presents with the following radiological patterns: 1) clear-cut erosions of the articular surfaces, especially in the distal interphalangeal joints of the hands and in the metatarso-phalangeal joints of the feet, with symmetrical distribution (not necessarily); 2) osteolytic punched-out areas in the epiphyseal spongiosa, ranging in size from 1 mm to over 1 cm; 3) no osteoporosis, no osteo-proliferative or periosteal reactions, not even in the presence of large osteoarticular destructions; 4) frequent atlanto-epistropheal subluxation; 5) articular ankylosis at the sacroiliac joints only. The association of the above patterns and the relatively benign clinical course distinguish multicentric reticulohistiocytosis from rheumatoid arthritis, psoriasis arthritis, erosive osteoarthritis, and gout. A reliable diagnosis can be suggested on the basis of radiological findings alone, even before cutaneous or mucosal lesions appear--which are, at any rate, not sure to appear and typical of nails only. An unquestionable diagnosis can be made at histology of synovial and/or cutaneous nodules. Multicentric reticulohistiocytosis is considered an uncommon condition (nearly 100 cases in international literature to 1989); the authors believe it to be commoner though often misdiagnosed as a "variant of rheumatoid arthritis". | |
| 2811200 | [The risk of developing osteoporosis in rheumatic diseases]. | 1989 Jul | Although osteoporosis is frequently associated with rheumatic diseases, at present it is not clear if osteoporosis is due to local factors as for example disease and inflammation, or to a systemic factor related to the rheumatic condition. In this review the present stage of knowledge on the inverse relationship between primary osteoarthritis and primary osteoporosis, bone metabolism in rheumatoid arthritis, the effect of corticosteroids on bone in rheumatoid arthritis, and preventive and curative therapy for osteoporosis is discussed. | |
| 20144117 | Dispersed cell cultures of rheumatoid synovial membrane. | 1987 | The morphology of cells released from synovial tissues involved by rheumatoid arthritis and grown in tissue culture has been described. Cultures were well established and differentiated by the third day. In growth medium enriched with heat-inactivated rheumatoid serum syncytial cell masses frequently formed. This change particularly, was very like the characteristic cytopathic effect of a number of viruses. | |
| 20144119 | Electrodiagnostic investigation of the neuromuscular lesions in rheumatoid arthritis. | 1987 | 85 muscles, in 30 rheumatoid arthritis patients, were examined by EMG. The patients were subdivided into steroid and non-steroid treated groups. In both groups, the EMG demonstrated myopathic, polymyositic and neuropathic lesions, the first two predominating. Similar changes were found regardless of the size of the muscle examined. A relatively high number of myopathic changes were observed in early RA. The activity of the disease did not seem to play an important role. No correlation with the condition of the adjacent joint was found. No patient had a normal EMG in all the muscles examined. The conduction velocity indicated an entrapment syndrome in four patients. Creatine phosphokinase was normal in 23 patients, while aldolase was moderately high in 9 of 23 cases. | |
| 3822493 | The Varni/Thompson Pediatric Pain Questionnaire. I. Chronic musculoskeletal pain in juveni | 1987 Jan | The Varni/Thompson pediatric pain questionnaire (PPQ) represents an attempt to empirically assess the complexities of pediatric chronic, recurrent pain. This initial investigation targeted chronic musculoskeletal pain in children with juvenile rheumatoid arthritis. The PPQ provides a developmental step toward the comprehensive assessment of the pain experience in children with chronic pain. Further reliability and validity studies are needed to determine the generalizability of the PPQ with larger numbers of children with a variety of acute and chronic pain experiences across a diversity of settings. | |
| 2948515 | Defect of suppressor cell induction in patients with juvenile rheumatoid arthritis. | 1986 Dec | We studied the suppressor cell activity induced by concanavalin A (Con A) in 9 patients with acute febrile juvenile rheumatoid arthritis (JRA). The suppressor activity of JRA patients was higher than that of normal controls. However, the activity was significantly reduced by treating Con A-activated cells with mitomycin C (MMC) (P less than 0.05). On the other hand, the suppressor activity of normal controls and systemic lupus erythematosus (SLE) patients was not affected by MMC treatment. Two of 5 SLE patients showed low activity even before MMC treatment. The addition of the culture supernatant of Con A-stimulated peripheral blood mononuclear cells from a normal donor restored the induction of suppressor activity of JRA which was decreased by MMC treatment. The results indicated that patients with acute febrile type of JRA had reduced MMC resistant suppressor cell activity and that this was due to a defect in the ability of the cells to produce soluble factors needed to induce MMC resistant suppressor cells. | |
| 3778542 | Complement activation in synovial fluid and tissue from patients with juvenile rheumatoid | 1986 Nov | Synovial fluid (SF) and synovial tissue from 10 patients with juvenile rheumatoid arthritis were examined. The SFs were heterogeneous with respect to the degree of complement activation. Quantification of C3dg and the terminal complement complex revealed a positive correlation between activation of the early and the late parts of the cascade in all patients. The amount of C-reactive protein and the number of white blood cells in the SF correlated significantly with the degree of complement activation. Weak deposits of C3, C3dg, or terminal complement complex were observed in a few vessels in the synovial tissue from 5 of the patients. There was no correlation between complement activity in SF and in the corresponding tissue. Furthermore, there was no correlation between clinical activity in the joints and the degree of complement activation. It is concluded that there is a discrepancy between synovial tissue and synovial fluid with respect to complement activation. C-reactive protein may, to some extent, be responsible for activation in SF, and the accumulation of white blood cells may be due to complement activation products. | |
| 2313675 | Antinuclear antibodies in arthritic and nonarthritic children with uveitis. | 1990 Jan | We determined whether a positive test for antinuclear antibodies correlated with uveitis only in children with juvenile rheumatoid arthritis (JRA) or whether they also represented a serologic marker for isolated idiopathic chronic uveitis in children. We conclude that the immunopathogenesis of uveitis associated with JRA is different from that of idiopathic chronic uveitis. |