Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 1700718 | Activation of T cell subsets in the peripheral blood of patients with Sjögren's syndrome. | 1990 Nov | Using 3-color flow cytometry, we determined the proportions of activated T cells (DR+), including CD4+ cells, CD8+ cells, and their subsets, in the peripheral blood of 17 patients with Sjögren's syndrome (SS). Activated T cells were significantly increased in CD4+ cells, CD8+ cells, and T suppressor inducer (CD4+, Leu-8+), T helper (CD4+, Leu-8-), and T cytotoxic (CD8+, CD11-) subsets, but not the T suppressor/natural killer subset (CD8+, CD11+), in patients with SS as compared with the controls. Furthermore, the proportions of activated T cells in the CD4+, Leu-8- subset, the CD8+ subset, or the CD8+, CD11- subset showed a positive correlation with serum gamma globulin levels in the SS patients. Our findings suggest that a certain immunoregulatory balance between T helper and T suppressor activities is maintained in SS patients, although this balance seems to occur at highly activated levels, and that quantitative changes of some lymphocyte subsets are important factors in maintaining this balance. We discuss the possibility that CD4+, Leu-8+ cells recirculate into peripheral lymph nodes, while CD4+, Leu-8- cells migrate into inflammatory tissues such as salivary glands, since the Leu-8 antigen is reported to be a homing receptor in peripheral lymph nodes. This process might be accelerated in SS, and each T cell subset may further participate in immunologic activation in the lymph nodes or target tissues. | |
| 2500291 | Immunosialadenitis (Sjögren's syndrome) and lymphoproliferation. | 1989 Mar | The association of primary salivary gland non-Hodgkin's lymphoma (NHL) and immunosialadenitis (myoepithelial sialadenitis, MESA) is well recognized. Within MESA the whole spectrum of lymphoproliferation starting with a prelymphoma transforming into an early lymphoma and later on into a manifest lymphoma can be observed. These lymphomas represent so-called low grade B-cell lymphomas of mucosa associated lymphoid tissue (MALT), an entity also including lymphoplasmocytoid immunocytoma according to the Kiel classification of NHL. In a few patients a transition into a high grade B-cell lymphoma may occur. The recognition of early stages of lymphomas and their distinction from reactive MESA is only possible by application of immunohistological methods. | |
| 1696100 | [Sialochemical studies in Sjögren disease]. | 1990 Jun | The concentrations of sodium, potassium, calcium, protein and lactoferrin and the enzymatic activity of salivary kallikrein, phosphohexoisomerase, alpha-amylase, lysozyme and IgA were measured in the parotid saliva of 12 patients suffering from Sjögren's syndrome. Compared with a control group (n = 31) remarkable differences were found in the composition of the parotid saliva in Sjögren patients. The possible reasons for these sialochemical alterations are discussed. | |
| 2528961 | Molecular cloning of cDNAs expressing SS-B/La protein. | 1989 Aug | Using serum from a patient with Sjögren's syndrome containing a high titer of anti-SS-B/La antibody, cDNA clones (a representative clone was called pA158) were isolated from a human fibroblast cDNA library in lambda gt11 expression vector. After subcloning of pA158 cDNA into an expression plasmid vector pEX-2, a large amount of the recombinant fusion protein with cro-beta-galactosidase (called pA158EX) was obtained in E. coli culture containing the recombinant pEX-2. Antibodies against pA158EX were purified from the patient serum by Sepharose 4B conjugated with the purified pA158EX protein. Immunofluorescent staining of HEp-2 cells with the anti-pA158EX antibodies showed a speckled nuclear staining. In immunoblot analysis, the anti-pA158EX antibodies reacted with 50 kDa protein that was compatible with SS-B/La protein. Immunoprecipitation of leukocyte lysate with the anti-pA158EX antibodies and the following RNA analysis showed that the antibody precipitated Y5 RNA. These findings indicate pA158 is a cDNA for SS-B/La protein. The purified fusion protein was used for enzyme-linked immunosorbent assay (ELISA). Optical density values of anti-SS-B positive sera were high, but those of anti-SS-B negative sera and healthy donor sera were low. In the Northern blot using human RNA and pA158 cDNA, a single band about 1.8 kb was recognized. A full-length cDNA was further obtained by screening of pcD library using pA158 cDNA as a probe. | |
| 3458307 | Gene interaction at HLA-DQ enhances autoantibody production in primary Sjögren's syndrome | 1986 May 30 | Primary Sjögren's syndrome is an autoimmune disorder characterized by dryness of the mouth and eyes. The human leukocyte antigen (HLA) locus DQ is related to the primary Sjögren's syndrome autoantibodies that bind the RNA proteins Ro/SSA and La/SSB. Both DQ1 and DQ2 alleles are associated with high concentrations of these autoantibodies. An analysis of all possible combinations at DQ has shown that the entire effect was due to heterozygotes expressing the DQ1 and DQ2 alleles. These data suggest that gene interaction between DQ1 and DQ2 (or alleles at associated loci), possibly from gene complementation of trans-associated surface molecules, influences the autoimmune response in primary Sjögren's syndrome. | |
| 2784865 | Circulating IgA- and IgM-rheumatoid factors in patients with primary Sjögren syndrome. Co | 1989 | IgM- and IgA-rheumatoid factors (RF) were determined by ELISA technique in 40 consecutive patients with primary Sjögren syndrome (primary SS), all fulfilling the Copenhagen diagnostic criteria. Increased concentrations of IgM-RF were found in 25 patients (63%) (median concentration: 85 IU/ml, range 11-302 IU/ml). Increased concentrations of IgA-RF were found in 22 of the patients (55%) (median concentration: 100 AU/ml, range 25-255 AU/ml). The levels of IgM- and IgA-RF were closely correlated (r = 0.82, p less than 0.001). IgM-RF (p = 0.037), but not IgA-RF (p = 0.17), was significantly correlated to the presence of antinuclear antibodies (ANA). In contrast, IgA-RF (p = 0.002), but not IgM RF (p = 0.068), was significantly correlated to the presence of hypergammaglobulinemia. The most common extraglandular manifestations were: arthralgia/synovitis (60%), myalgia/myositis (28%), Raynaud's phenomenon (20%) and pulmonary manifestations (15%). Concentrations of both IgM-RF (r = 0.43, p less than 0.01) and IgA-RF (r = 0.57, p less than 0.001) were correlated to the number of extraglandular manifestations present in each patient. | |
| 3946996 | Primary lymphomas of the major salivary glands. | 1986 Jan | The finding of a lymphoma of the major salivary glands (almost always parotid gland) has two diagnostic implications; it is only part of a known disseminated process, or it may be the first clinical-pathologic evidence of lymphoma. In the latter instance, the disease may be localized or, most often, be a precursory lesion to regional or systemic disease. Patients with Sjögren's or sicca syndrome are at increased risk for the development of lymphoma, and their lymphomas are biologically more aggressive. | |
| 3760566 | IgG subclasses of autoantibodies in systemic lupus erythematosus, Sjogren's syndrome, and | 1986 Oct 15 | The IgG subclasses displayed by autoantibodies were examined in patients with systemic rheumatic diseases. Solid-phase assays performed with purified antigens were combined with a set of four mouse monoclonal antibodies specific for each human subclass to provide quantitative data for all the major autoantibody specificities. IgG1 accounted for an average of 55% of the total antibody activity to native and denatured DNA, Sm antigen, and histone and constituted significantly more anti-SS-B and anti-nRNP (84% and 92%, respectively). The remaining antibody activity consisted largely of IgG3, and this subclass was particularly prominent with anti-histone and anti-Sm in patients with systemic lupus erythematosus. In contrast, IgG2 constituted 3 to 12% of the anti-native and anti-denatured DNA and less than 5% of the anti-SS-B/La activity in only three patients with Sjogren's syndrome. IgG2 was essentially undetectable in antibodies to Sm and RNP antigens. IgG4 was also uncommon, although this isotype was significantly more prevalent in anti-histone from patients treated with procainamide showed that the isotype distribution of anti-histone and anti-denatured DNA remained remarkably constant. However, during periods of large increases in autoantibody activity, a shift from predominantly IgG3 to predominantly IgG1 occurred, consistent with the interpretation that there might be a sequential activation of heavy chain constant regions as the immune response matures. The disproportionately high levels of IgG1 and IgG3 displayed by all the autoantibody specificities examined may indicate that a common immunogenic feature of autoantigens or a common control mechanism underlies the regulation of autoantibody expression. | |
| 3517176 | Detection of circulating Yersinia-immunoglobulin complexes by enzyme immunoassay (EIA). | 1986 May 22 | An enzyme immunoassay (EIA) was developed for the detection of Yersinia-immunoglobulin complexes of known Ig class. Immune complexes (ICs) were attached to polystyrene microtiter plates by rabbit anti-human immunoglobulins, and the existence of Yersinia enterocolitica O:3 antigens was demonstrated using Fab fragments of alkaline phosphatase (AP)-conjugated antibody against the same serotype. Simultaneous binding of Yersinia antigens and immunoglobulins was a prerequisite for the detection of ICs. The method will be valuable for research into the immunopathogenetic mechanisms leading to reactive arthritis after Yersinia infection. | |
| 2749166 | [Changes in the locomotor system in inflammatory intestinal diseases. III. Clinical aspect | 1989 Mar | This investigation is a follow up study of our previous study (Sborn. lék., 90, 1988, 140-146). In the same group of 119 patients with inflammatory intestinal diseases (Crohn's disease - 37, ulcerative colitis - 50, celiac sprue - 32) we followed further affection of locomotor system, peripheral arthritides. Inflammatory manifestations in peripheral articulations were manifest in 11 (9.2%) patients. Among common features of enteropathic arthritides belong: asymmetrical mono-oligoarthritis, in 67% of cases in lower extremities, without X-ray structural manifestations - erosive changes in the whole course, without functional deficit, inflammatory nature of the exudate, absence of rheumatoid nodes, RF negativity, episodic course. On the contrary in 3 (27%) patients the course was chronic. Incidence, relapse, activation of arthritis depends in 18% on activity and in 17% on intestinal complications of the underlying disease. Surgical treatment of small and/or large intestines lead in 60% of cases to remission of peripheral arthritis. In 1 patient (with celiac sprue) synoviorthesis with radioactive coloid 90Y was performed. | |
| 3626848 | Large granular lymphocyte leukemia. Report of 38 cases and review of the literature. | 1987 Sep | LGL leukemia results from a chronic, clonal proliferation of LGL. Chronic neutropenia with recurrent bacterial infection and splenomegaly are common clinical manifestations. Rheumatoid arthritis coexists in some of these patients, who thus resemble patients with Felty syndrome. Other hematologic abnormalities that may occur include pure red-cell aplasia and adult-onset cyclic neutropenia. Lymphoid infiltration of bone marrow, splenic red pulp cords, and hepatic sinusoids is characteristic; lymph node and skin involvement are rare. Multiple serologic abnormalities are frequently present, including positive tests for rheumatoid factor and/or antinuclear antibody, polyclonal hypergammaglobulinemia, and circulating immune complexes. Antineutrophil and antiplatelet antibodies are often present. Leukemic LGL exhibit phenotypic heterogeneity; the most common phenotype in our patients is CD2+, CD3+, CD8+, HNK-1+, CD16-. Despite markedly increased numbers of LGL, functional activity of the cells is usually decreased. The mechanism of cytopenias is uncertain: in pure red-cell aplasia, it appears to be due to suppressive effect on erythropoiesis by abnormal LGL, but in patients with chronic neutropenia it may be antibody-mediated. Although most patients appear to have a relatively benign clinical course, mortality from infections and progressive lymphoproliferation is substantial. Optimal therapy remains undefined. Some preliminary evidence suggests that LGL leukemia may be associated with infection with a retrovirus similar to HTLV-I. Although relatively rare, LGL leukemia is of interest because a better understanding of this disease process may contribute to our knowledge of autoimmune diseases, the immunoregulatory functions of LGL, and the mechanisms controlling normal hematopoiesis. | |
| 3329108 | Evaluation of tenoxicam in rheumatology--clinical trial results in Argentina and Brazil. | 1987 | The therapeutic activity of tenoxicam, a thienothiazine derivative with analgesic and anti-inflammatory properties, has been studied by 15 investigators in Argentina and Brazil. Twenty-nine clinical trials were performed in a total of 747 patients suffering from rheumatoid arthritis (270), cox- and gonarthrosis (190), extra-articular inflammation (250) and acute gout (37). Out of the patients studied, 507 received tenoxicam and 240 were given comparative preparations. In 76% of the patients 20 mg tenoxicam was given as a single daily dose. In most patients duration of treatment was either six weeks or six months. Therapeutic results were evaluated according to the evolution of pain in various conditions as well as that of the articular, clinical and functional status. Once treatment was concluded a global evaluation of efficacy and tolerance was performed. The statistical analysis showed a significant improvement, in comparison to baseline, in all parameters considered under the different conditions. Double-blind studies showed no significant statistical differences between tenoxicam and the comparative preparations. Tolerance to tenoxicam was considered excellent, granting that some patients referred to adverse effects of the gastrointestinal type, such as epigastric discomfort, pyrosis and flatulence of moderate intensity. Tenoxicam is a new non-steroidal anti-inflammatory compound which is well tolerated and has excellent activity in the treatment of diverse rheumatoid conditions. | |
| 1771320 | [Wegener's granulomatosis disclosed by clinical symptoms of Horton's disease]. | 1991 Sep | In a series of 60 patients with Wegener's granulomatosis, 2 had initially presented with clinical signs suggestive of temporal arteritis. One of these two patients was a 69-year old woman suffering from inflammatory pain in the shoulders, wrists and knees, myalgias in the lower limbs and intermittent jaw claudication. The other patient was a 60-year old man with febrile polyarthritis predominantly affecting the knees and shoulders, and hyperaesthesia of the scalp. In both cases biopsy of the temporal artery gave negative results. Corticosteroids provided a dramatic improvement, but a relapse corrected the diagnosis. Three similar cases have been reported, but only one had a histological lesion of the temporal artery. Cases of temporal arteritis associated with pulmonary granulomatosis raise the problem of classification with localized Wegener's disease. An initial presentation suggestive of temporal arteritis may hide other systemic diseases, notably rheumatoid arthritis, periarteritis nodosa or Chug and Strauss angitis; Wegener's granulomatosis must be added to this list. | |
| 1656616 | Topical capsaicin in dermatologic and peripheral pain disorders. | 1991 Apr | Topical capsaicin has been introduced in the U.S. and Canada as a cream indicated for temporary relief of neuralgia following episodes of herpes zoster infections and in the treatment of diabetic neuropathy. Although capsaicin is clinically used as an external analgesic for temporary relief of neuralgia, it has also been widely used as a research tool to study peripheral pain. Capsaicin apparently works to release substance P from sensory nerve fibers and after repeated applications, depletes neurons of substance P. Clinical investigations of topical capsaicin include trials in chronic pain syndromes such as postherpetic neuralgia, postmastectomy neuroma, reflex sympathetic dystrophy syndrome, diabetic neuropathy, rheumatoid arthritis, psoriasis, hemodialysis-associated itching, and vulvar vestibulitis. In addition, therapeutic benefits of capsaicin cream on apocrine chromhidrosis have been described. Further clinical studies are warranted in several of these conditions to establish the efficacy of topical capsaicin. Serious or unexpected adverse reactions from clinical use have not been reported to date. Considering the paucity of safe and effective treatments for the conditions mentioned above, capsaicin cream appears to warrant further clinical investigations to establish its efficacy in a variety of chronic pain syndromes. | |
| 2041168 | [Thrombotic thrombocytopenic purpura associated with myasthenia gravis]. | 1991 Mar | A case of thrombotic thrombocytopenic purpura (TTP) associated with myasthenia gravis (MG) is reported. A 56-year-old woman was admitted to our hospital on April 27, 1988, because of easy fatigue and double vision. She was diagnosed as having myasthenia gravis from her neurological and laboratory findings. On the 14th hospital day, she developed fever, jaundice, hematuria, purpura and consciousness disturbance. Hematological examination revealed marked anemia, thrombocytopenia, fragmented red blood cells, elevated bilirubin and LDH level. Coagulation studies were almost normal. She was diagnosed as having TTP and treated with corticosteroid, antiplatelet agents and plasma exchange. Clinical condition and laboratory findings improved by the 23rd hospital day. It has been reported that TTP is associated with various autoimmune disease such as systemic lupus erythematosus, rheumatoid arthritis, Sjögren's syndrome or idiopathic thrombocytopenic purpura. However TTP associated with MG appears the first report. This case may suggest that one of pathogenesis of TTP is autoimmune mechanism. | |
| 2147330 | [The interaction between synovial cytokines and peripheral nerve function: a potential ele | 1990 Sep | Cytokines of rabbit synovial origin were injected into 6 rats under the epineurium of the sciatic nerve. Five controls were injected with similar preparations lacking cytokines. After injection, rats were examined for a period of 7 days. For neurophysiological evaluation the tibial branch of the sciatic nerve was stimulated with supramaximal voltage impulses of constant duration. The responses were recorded at the dorsal root entry zone L1. F-wave latencies were recorded at the distal hind paw after stimulation of the tibial nerve. Filters were set at LF: 20 Hz, HF: 10 kHz. Synovial cytokines caused a significant decrease in amplitude and increase in latencies of the recorded nerve potentials. Our results indicate that interleukin-1, which is a major component of the synovial cytokine preparation, could play an important role in degenerative spine disease through a damaging effect on nerve function. This action would explain why radiculopathy and pain can develop without signs of nerve compression. The results are also relevant to patients suffering from osteoarthritis and rheumatoid arthritis. | |
| 1698985 | Anticardiolipin and antineutrophil antibodies in giant cell arteritis. | 1990 Jul | IgG anticardiolipin antibodies (aCL) were found in a patient with Takayasu's arteritis and then in 11 of 22 patients with temporal arteritis (TA) studied prospectively. In comparison IgG aCL were found in only one of 18 elderly controls with rheumatoid arthritis and one of 47 healthy elderly controls. In TA IgG aCL were more frequent in those with acute disease (8/12), in those with an accompanying history of polymyalgia rheumatica (9/13), p less than 0.05); and levels normalized with corticosteroid treatment. Antineutrophil cytoplasmic antibodies (c-ANCA) were present in one patient with TA who had no systemic disease. | |
| 2295184 | Experience with bipolar prosthesis in femoral neck fractures in the elderly and debilitate | 1990 Feb | Eighty-eight bipolar Bateman hip endoprostheses for medial femoral neck fractures were implanted. The average age of the patients was 75 years. Intrahospital results proved the morbidity and mortality rates to be well within acceptable limits. Thirty patients were followed during periods of 12-74 months (median, 33 months). According to Charnley evaluation, mobility was excellent and very good in 20 patients (86%). Good function was present in 63% of patients. The majority of patients belonged to category C as defined by Charnley. Mild pain was present in 19 patients (63%); in only two cases, involving severe rheumatoid arthritis, pain was clearly related to sinkage. Radiographically, no visible protrusion or socket wear was present. Periarticular ossification occurred in 19 patients, but this did not impair function. | |
| 1708848 | Titer and specificity of autoantibody to beta 2-microglobulin in sera from patients with r | 1990 | Sera from patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and mixed connective tissue disease (MCTD) possessed higher titer of antibody to human beta 2-microglobulin (beta 2m) than those from healthy controls and patients with Behçet's disease in the enzyme-linked immunosorbent assay. It was also confirmed by the immunoprecipitation method. Anti-beta 2m antibody in sera from those patients immunoprecipitated free beta 2m but not beta 2m in association with major histocompatibility complex class I antigen heavy chain. It was suggested that anti-beta 2m antibody in sera from either patients or healthy controls might be directed mainly against free beta 2m. The relationship between the anti-beta 2m antibody and anti-lymphocytotoxic antibody found in those patients is discussed. | |
| 2788544 | A survey of nonatopic and atopic children and adults for the presence of anti-IgE autoanti | 1989 Oct | The prevalence of naturally occurring anti-IgE autoantibodies was assessed by surveying 387 sera from atopic, nonatopic, and autoimmune patients. A significant number of children (28.5%) and adults (20%) with no history of allergy had high levels of autoanti-IgE. The level of autoantibodies to IgE in children with clinical asthma or atopic eczema was not significantly elevated over normal. Similarly, adults with atopic asthma, allergic rhinitis, or urticaria or sera from individuals with rheumatoid arthritis or systemic lupus erythromatosis showed no significant elevation of auto-anti-IgE. In contrast, 82% of adults with eczema had medium to high levels of auto-anti-IgE and the mean concentration in sera was significantly (P less than 0.01) raised. The relevance of auto-anti-IgE in atopic eczema is discussed. |