Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 181972 | Adjuvant disease induced by mycobacteria, determinants of arthritogenicity. | 1976 Feb | Genetic, endocrine and immunological factors are probably involved in adjuvant polyarthritis. The nature of the vehicle and of the mycobacterial components administered also has a major influence. It was originally assumed that arthritogenicity and adjuvanticity of mycobacterial fractions such as wax D were intimately related. Our previous findings showed that the water soluble adjuvant (WSA) of M.smegmatis which could substitute for mycobacterial cells in Freund's complete adjuvant and induce delayed hypersensitivity was not arthritogenic in the Wistar rat. We have since observed that auto-immune diseases could be elicited by WSA. Therefore experiments were repeated using the very susceptible Lewis strain. The activity of cord factor and of various mycobacterial preparations suspended in mineral or in peanut oil was also evaluated in mice and in normal or hypophysectomized rats. Our present findings confirm the absence of arthritogenicity of WSA in the Lewis strain. They also indicate that cord factor with WSA does not suffice to induce a generalized adjuvant disease, but that a mycobacterial component which could be susceptible to lysozyme treatment is required also. However, the local inflammation of the injected limb was produced by a preparation of cord factor administered in mineral or even in peanut oil. This was observed in normal or hypophysectomized rats and in Swiss mice which were not susceptible to the generalized disease. | |
| 7330168 | Rheumatoid cervical myelopathy. | 1981 Summer | The mode of presentation and the factors which influenced the diagnosis and outcome in 31 patients with rheumatoid cervical myelopathy were reviewed. The presenting features included paraesthesiae and /or numbness (23 cases) weakness (six) flexor spasms (five) and incontinence (two). Isolated sensory loss presenting in a glove and stocking distribution was often misdiagnosed as a peripheral neuropathy. Multiple neurological deficits were eventually present in all patients and included spastic quadriparesis (17), spastic paraparesis (seven), bladder disturbance (nine), and cranial nerve deficits (six). Twenty-seven patients had impaired light touch and pin prick sensation but the sensory level did not correlate with the presumed level of spinal cord compressions. Twenty-one patients had dissociation between loss of position and vibration sensation. All patients had one or more cervical luxations; five patients had atlanto-axial luxation alone, five had subaxial luxation alone and the remainder multiple luxations. Neurological improvement was more frequent and of longer duration in those treated by occipito-cervical fusion than in those treated conservatively but fitness for surgery may have selected a group with a better prognosis. | |
| 537026 | Detection of soluble immune complexes by the technique of ADCC inhibition in human disease | 1979 Nov | Soluble immune complexes were detected using inhibition of antibody-dependent cell-mediated cytotoxicity (ADCC) in sera of patients with various diseases. Results were positive in 32/41 patients with rheumatoid arthritis (78%), in 27/38 systemic lupus erythematosus patients (71%), in 7/10 cutaneous lupus erythematosus patients (70%), in 6/8 mixed connective tissue disease patients (75%), in 11/26 membranous glomerulonephritis patients (42%), in 6/20 membranoproliferative glomerulonephritis patients (30%) and in 3/12 multiple sclerosis patients (25%). ADCC inhibition was compared with PEG precipitation technique and was found to be more sensitive for detecting soluble immune complexes. Various pitfalls are discussed. | |
| 640750 | Early rheumatoid-like lesions in rabbits injected with foreign serum: relationship to loca | 1978 | Immune complex localization in joint tissues and white cell effusion into the joint space as well as synovial lesions are shown to occur in rabbits injected intravenously with bovine serum. Synovial lesions of one knee were found to be associated with immune complexes in joint tissues of the contralateral knee (because of the experimental design very few rabbits had both immunofluorescence and histology performed on the same joint). There was also a week correlation between moderate and gross synovial lesions and cellular effusions of the same joint. Cartilage proteoglycan depletion was seen in some injected animals and this was related to lining cell hyperplasia. Rabbits from different breeding colonies displayed different incidences of lesions suggesting an environmental or genetic influence on the development of rheumatoid-like lesions. | |
| 3862730 | Enhanced phospholipase A2 and C activities of peripheral blood polymorphonuclear leukocyte | 1985 Nov | Increased concentrations of eicosanoids are found in synovial fluids (SF) from patients with rheumatoid arthritis (RA). SF polymorphonuclear leukocytes (PMN), which derive from peripheral blood, usually account for approximately 90% of the cells in RA SF. Since eicosanoid precursor fatty acids (FA) are liberated by phospholipases from membrane phospholipids, we examined phospholipase A2 and C activities in peripheral blood PMN from patients with RA. Peripheral blood PMN from patients with RA (RA-PMN) exhibit greater phospholipase A2 activities against phosphatidylcholine (PC) and phosphatidylethanolamine (PE), and greater phospholipase C activities against PC, PE, and phosphatidylinositol (PI) than PMN obtained from normal volunteers (N-PMN). | |
| 6603297 | A comparative study of complement components in polyethylene glycol precipitated immune co | 1983 Jun | Circulating immune complexes and complement components C1q, C3 and C4 were measured following polyethylene glycol precipitation of serum from patients with ovarian cancer (OC), patients with rheumatoid arthritis (RA) and control patients (CP). The C3 and C4 content of the precipitated material was significantly greater in those patients with OC in comparison to those with RA or CP, while the C1q content did not differ significantly between the two groups of patients. A statistically significant correlation between IgG, C3 and C4 levels was found in the immune complexes of both groups of patients. C1q levels correlated significantly with IgG immune complex levels in patients with RA and CP, but did not in patients with OC. These data illustrate that immune complexes from patients with OC differ from complexes precipitated from RA sera and normal sera in their complement content. It is suggested that the largely unsuccessful attempts to detect immune complexes in sera from patients with ovarian cancer, using assays dependent on the binding of C1q may be related to differences in solubility of complexes from these patients in comparison to complexes present in non-neoplastic conditions. | |
| 312567 | In vitro studies on lymphocytotoxicity to synovial cells and Chang cells in rheumatoid art | 1979 Mar | Lymphocytotoxicity to autologous or allogeneic synovial cells and Chang cells was studied in 27 patients with rheumatoid arthritis (RA), in 5 patients with osteoarthrosis of the hip or knee and in 17 healthy controls. Ficoll gradient-separated lymphocytes from the peripheral blood, T cells and non-T cells were used as effector cells. T lymphocytes were isolated as E-rosette forming cells 10 percent of which carried Fc- receptors. The differential counts for T and B cells in the peripheral blood of the RA and osteoarthrosis patients were approximately the same as in the blood of the healthy controls. The counts of Fc-receptor-bearing cells in the RA patients were, however, significantly higher. Cytotoxic reactivity of lymphocytes from RA patients, osteoarthrosis patients or healthy controls to synovial cells of autologous or allogeneic origin could not be demonstrated in our study, in which 125I-iododeoxyuridine labelled target cells were used in the microcytotoxicity test of Cohen et al. However, lymphocytes of the peripheral blood showed an increased cytotoxicity to Chang cells, an effect for which Fc-receptor bearing cells were responsible. Serum did not affect the cytotoxicity of lymphocytes. The results are interpreted as demonstrating an enhanced natural killer (NK) cell activity in RA patients; they do not indicate a specific cell mediated immune reaction to synovial cells. | |
| 6975682 | Immune complex-dependent T cell-mediated cytostasis (IDTC). | 1981 Jul | The growth of human lymphoid cell lines was suppressed by human peripheral T cells in the presence of immune complexes (IC). T cells bearing IgG receptors (TG cells) were, but T-non-G cells and non-T cells were not, active as effectors of cytostasis which was induced by IC-mediated bridging of the target and effector cells via Fc receptors. We propose that this phenomenon be called 'immune complex-dependent T cell-mediated cytostasis' (IDTC) and suggest its possible role in IC-mediated tissue injury, especially where T cell infiltration is observed. TG cells from healthy individuals suppressed Ig production and were cytotoxic effectors upon interaction with IC. However, the cytotoxic effectors seem to be distinct from the suppressors of Ig production since TG cells from rheumatoid arthritis patients showed dissociation between these two functions: they displayed cytostasis but had only little suppressive effect on the B cell response. | |
| 1124955 | Acute knee joint rupture after yttrium 90 injection. | 1975 Feb | Two cases of acute rupture of the knee joint are reported after the intra-articular injection of radioactive yttrium 90. It is suggested that this may be a complication of intra-articular radioactive injections. | |
| 7046292 | [Coincidence of lethal B-streptococcal disease of newborn with acute puerperal polyarthrit | 1982 | Reported in this paper is a lethal B-streptococcal disease of a newborns which grew manifest as meningitis. It had been caused by Type Ic B-streptococci. The same pathogen was isolated from the mother's vaginal secretion. The mother fell sick with acute allergico-infectious polyarthritis, ten days after birth. This coincidence between infection of the newborn and the mother's puerperal disease is discussed. | |
| 6363217 | Bacteriuria and primary biliary cirrhosis. | 1984 Feb | Significant bacteriuria was found in 19% of 87 women with primary biliary cirrhosis, whereas in 89 women with other types of chronic liver disease bacteriuria was present in only 7%. In 74 women with rheumatoid arthritis 8% were bacteriuric. Midstream urine specimens obtained from 144 consecutive women with primary biliary cirrhosis attending hospital over a two year period showed that 50 (35%) developed bacteriuria during 12 months of follow up. Bacteriuria was unrelated to age, raised serum bilirubin, drug therapy or urinary pH but was more common in patients with late stage (fibrotic) disease as judged by histological criteria. Fifty seven per cent of bacteriuric primary biliary cirrhosis patients suffered more than one urinary infection. Fifty nine per cent of the 156 bacteriuric episodes were asymptomatic. The types of organism isolated, the antibiotic sensitivity patterns and cure rate were similar to those reported in bacteriuric women without other underlying disease. The reinfection rate (34%), however, was double that reported for bacteriuric episodes in 'problem' women with recurrent bacteriuria, indicating a special susceptibility to urinary infection. The most common isolates were E coli (70%), which did not show abnormal adhesiveness to uroepithelial or buccal cells of normal women, or to those of primary biliary cirrhosis patients. Patients with primary biliary cirrhosis have not been reported to be more susceptible to infection in general. Bacteriuria, however, was common throughout all clinical stages of primary biliary cirrhosis. Thus there may be a unique association between bacteriuria and primary biliary cirrhosis. | |
| 6893815 | Examination of prevalence rates of possible risk factors in a population with a fracture o | 1980 Nov | No statistical increase in the prevalence of either diabetes, rheumatoid arthritis or primary hyperparathyrodism was found among the hip fracture patient population. Since the relative risks for these diseases is small, the statistics suggest that these conditions are either noncontributory or represent only a minor risk factor in the overall pathogenesis of hip fractures. About 20% of the hip fracture patients had a history of other identifiable risk factors such as thyrotoxicosis, hemiplegia, malabsorption syndromes and corticosteroid therapy. Of these conditions only thyrotoxicosis could be evaluated by comparison of prevalence rates, and a significant increase was found among the fracture patients. A highly significant correlation was found between the side of a previous hemiplegia and side of he subsequent hip fracture; this may be due to the development of disuse osteoporosis in the hemiplegic limb. Recent reports have shown that a reduction in the number of hip fractures is associated with a high calcium intake or prophylactic estrogen therapy. Preventive therapy in patients with hemiplegia, thyrotoxicosis, or other predisposing conditions leading to osteopenia might result in a further reduction of the hip fracture rate. | |
| 7425534 | Purification and quantities of immunoglobulins of rheumatoid synovial tissues. | 1980 Sep | Synovial tissue of rheumatoid arthritic origin was incubateed with radio-labelled amino acids and the immunoglobulin fraction of the products isolated using either a diethylaminoethyl (DEAE)-cellulose column or an affinity column of antihuman immunoglobulin coupled to Sepharose. The affinity column method provided a simple, one-step procedure for obtaining quantitative yields of substantially pure immunoglobulin. The elutions from the affinity columns utilized guanidine-HCl allowing the elutions to be performed quickly and under conditions which apparently did not denature the immunoglobulins except perhaps immunoglobulin M. Other solvent conditions for dissociating antibody-antigen complexes were shown to be not suitable and resulted in large volumes of eluates. The affinity columns could be reutilized many times without apparent loss of capacity to bind immunoglobulins. | |
| 52191 | Effect of the common bile acids on the fibrin/fibrinogen fragments in rheumatoid synovial | 1975 | Fibrin/fibrinogen degradation products observed in rheumatoid synovial fluid exhibit resistance to plasmin proteolysis. In the present study, the influence of the common bile acids on the plasmin digestion of these degradation products in 16 rheumatoid synovial fluids were quantitated immunologically by radial immunodiffusion, and qualitatively estimated by crossed immunoelectrophoresis. Addition of chenodeoxycholic acid, deoxycholic acid, and their taurine and glycine conjugates in concentrations of 3.33 mumole/ml of a mixture of rheumatoid synovial fluid and plasmin resulted in complete plasmin degradation. Cholic acid and its taurine and glycine conjugates were effective only in concentrations of 4.44 mumole/ml. A detergent, such as Triton X-100, had little or no effect on the plasmin digestion. Other proteins capable of influencing fibrinolytic activity, such as plasminogen and the inhibitors alpha1-antitrypsin and alpha2-macroglobulin, were not affected by the two detergents. The bile acids are thought to influence the fibrin/fibrinogen degradation products by unfolding the protein at a molecular level, by virtue of their properties as steroid detergents, leaving the fibrin/fibrinogen degradation products susceptible to plasmin digestion. | |
| 4640945 | Effect of rheumatoid factor on complement-mediated phagocytosis. | 1972 Dec | The frequency and amount of IgM rheumatoid factor (RF) in the blood of patients with rheumatoid arthritis (RA) correlate with the severity of the disease and the number of complications. Though previous studies of RF in subacute bacterial endocarditis have shown that RF inhibits phagocytosis of microorganisms by granulocytes, the presence of low levels of complement (C) in blood and synovial fluid of patients with the highest titers of RF suggests that an interaction between RF and C may contribute to the inflammatory process in RA. We thus employed a quantitative methodology to examine the effect of RF on complement-dependent phagocytosis of sheep erythrocytes by rabbit granulocytes. Addition of 2500 molecules of IgM RF to sheep cells heavily coated with IgG antibody (195,000 molecules per cell) resulted in virtually complete inhibition of uptake of C3 (beta(1c)) and prevention of phagocytosis, an effect resulting from inhibition of uptake of C1 by the cells. When erythrocytes coated with only 34,000 molecules of IgG antibody were employed, phagocytosis was similarly inhibited. However the effect of RF on such cells was shown to be primarily mediated through inhibition of C4 rather than C1 uptake. Although the results do not exclude the participation of an IgM RF of higher avidity, present only in the tissues in rheumatoid inflammation, circulating IgM RF probably does not play a potentiating role in rheumatoid inflammation. | |
| 6184021 | Effect of sera from patients with rheumatoid arthritis on normal lymphocytes: a possible i | 1982 Dec | The ability of rheumatoid sera to support concanavalin-A transformation of normal lymphocytes was inversely related to serum C1q binding activity. When C1q binding activity of the sera was removed by absorption with staphylococcal protein A, subsequent lymphocyte response increased to the level found in immune-complex negative sera. Gel filtration of a small number of sera suggested that the suppressive material had a molecular weight in the range 1.8-4.9 x 10(5) daltons. Aggregated human gammaglobulin suppressed con-A transformation of normal lymphocytes in a dose-dependent fashion. These results suggest that immune complexes present in rheumatoid sera can suppress lymphocyte responsiveness. The relevance of this observation to be clinical features of rheumatoid arthritis is discussed. | |
| 7362666 | The toxicity pattern of D-penicillamine therapy. A guide to its use in rheumatoid arthriti | 1980 Feb | One hundred and one patients with rheumatoid arthritis were followed prospectively to assess the efficacy and toxicity of therapy with D-penicillamine. After a mean total followup of 11.5 months (38 patients have completed 2 years of followup) there was a 70% overall improvement rate with 2 complete remissions. Sixty-one patients developed 84 separate toxic reactions, 36 of which required drug withdrawal. Skin rashes (27/84), proteinuria (15/84), low platelets (14/84), and taste abnormalities (10/84) were the most common side effects of therapy at a mean D-penicillamine dose of 463 mg/day. The majority of toxic reactions (85%) occurred in the first 6 months, but proteinuria and thrombocytopenia were more common in the 6 to 12 month treatment period. Previous gold toxicity was a risk factor for developing D-penicillamine toxicity (10/13). Our observations suggest that D-penicillamine related toxicity is a major problem even at 500 mg/day, but the drug can be used with an increased safety margin after 9 months of continuous therapy. | |
| 6671913 | Advantages of metal-backed acetabular components for a total hip replacement: a clinical a | 1983 | A group of 53 hips in which metal-backed acetabular components had been inserted with the use of bone cement were reviewed roentgenographically at an average of 6.5 years following insertion, with a minimum follow-up of 5 years. Of this group, two were excluded for special reasons. Among the remaining hips, with an average age of 41 years at the time of insertion, the incidence of acetabular component loosening was 6%. In fact, the only acetabular component loosening occurred in three patients whose surgery was done at the age of 25 years or younger. No patient over the age of 25 years demonstrated a loose acetabular component. By selecting the subset of patients age 45 years and under, we could make a comparison with a similar group of patients reported by Dorr and Takai, in whom acetabular components had been inserted without metal backing. The group with the metal-backed acetabular components had a statistically significant reduction in acetabular component loosening. These clinical data, taken in conjunction with the finite-element studies reported in Chapters 11 and 12, make a very strong case for the use of the metal-backed acetabular component. Our clinical practice, and our recommendation, is to use a metal-backed acetabular component in every cemented total hip replacement, if possible. | |
| 7455586 | Cyto-nucleo-nucleolar variables estimating the lymphocyte reactivity in chronic internal d | 1980 Oct | To estimate the degree of lymphocyte reactivity, 11 cyto-nucleo-nucleolar variables were determined in the lymphocyte population from 30 healthy blood donors and 103 patients with chronic internal diseases with immune component such as: rheumatoid arthritis, chronic hepatitis, Hodgkin's disease or chronic lymphocytic leukemia. The data obtained showed that all the cyto-nucleo-nucleolar variables investigated were different in chronic hepatitis from all the other groups, both as regards the relative and absolute areas (low for the cell and nucleus and high for the nucleoli), and as regards the ratios of cell/nucleus and nucleus/nucleolus areas. | |
| 6604065 | Effect of stimulated neutrophils from the synovial fluid of patients with rheumatoid arthr | 1983 Jul | Neutrophils from the synovial fluid (SFN) of 10 patients with active rheumatoid arthritis (RA) were investigated to determine the generation of oxygen intermediates (OI) (O2-, H2O2, OH .), chemiluminescence, and lysosomal enzymes (lysozyme and beta-glucuronidase). Lymphocytes from healthy individuals were cocultured at 37 degrees C for 17 hr with SFN from the patients and the number of OKT4+, OKT8+, and OKT3+ cells and the response to mitogens were determined. A markedly increased OI and slightly elevated lysosomal enzyme levels were observed in SFN from patients. Coculture of lymphocytes with SFN resulted in a decreased number of OKT4+ and OKT8+ cells and a greatly reduced response to Con A and mildly diminished response to PHA, while OKT3+ cells were not affected. The simultaneous addition of superoxide dismutase and catalase restored the impairment of monoclonal antibody reaction and lymphocyte responsiveness almost to control levels. It is suggested that the disturbed immunoreactivity of synovial fluid lymphocytes from RA patients may be due to increased OI generated by stimulated neutrophils. |