Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 811395 | [Circulating immune complexes in man; precipitation of immune complexes with polyethylene | 1975 Mar 3 | Use of an experimental model consisting of purified tetanal toxoid and sera of patients vaccinated with this antigen reveals that immunodiffusion characterisation of C1q bound to immune complexes (IC) precipitating with 2,5% PEG (6000) represents a very sensitive means of detection of such IC. The majority of sera of patients with disorders in which IC can be suspected gives positive reactions. Precipitates contain IgG and IgM bound to C1q and rheumatoid factor is constantly detected. The reaction is positive among 10% of healthy patients: the possibility of circulating IC free from any pathological cases is under study. | |
| 6353922 | Circulating immune complexes in pregnancy, preeclampsia, and autoimmune diseases: evaluati | 1983 Oct 1 | Sera from 86 individuals were tested for circulating immune complexes by the polyethylene glycol precipitation method and a Raji cell enzyme immunoassay (Raji-ELISA). These included normal nonpregnant control subjects, nonpregnant patients with autoimmune diseases, healthy women in the second and third trimesters of pregnancy, patients with preeclampsia, and women with pregnancies complicated by preexisting autoimmune diseases. Diseases such as systemic lupus erythematosus and rheumatoid arthritis were associated with increased levels of immune complexes in both pregnant and nonpregnant individuals. Circulating immune complexes were not observed in normal pregnancies or in preeclampsia. Although pregnancy itself is not an immune complex-associated state, the presence of immune complexes in autoimmune diseases may explain some of the complications observed during pregnancy in these patients. | |
| 1142009 | Selenium levels in human blood and tissues in health and in disease. | 1975 Aug | The levels of selenium in human sera and pancreatic, hepatic and synovial tissues were measured. An attempt was made to correlate the selenium level with certain disease states. Selenium was determined by nerutron activation analysis, using the 17.4 second half-life isotope 77mSe with a sensitivity of 2ppb. Serum-bound selenium was determined in normal individuals and individuals with various malignancies, and medical and surgical disorders. Tissue selenium was assayed in diseased and normal pancreases, livers, and synovial membranes. A wide variation was observed both in the serum selenium content of patients with a malignancy and in postmoren pancreatic and synovial showing histopathological changes. Significantly lower selenium values were observed in sera from cancer patients than from normal individuals. Higher values were generally observed in patients with primary neoplasms of the reticuloendothelial system. Higher tissue concentrations were obtained in synovia from patients with rheumatoid arthritis and in pancreatic tissues associated with histopathological changes. | |
| 6753142 | [The introduction of non-steroidal antirheumatics: is there a rational therapy]. | 1982 Aug 28 | The pharmacologic properties, clinical efficacy and tolerability of the newer non-steroidal anti-rheumatic drugs are compared. Guidelines for a prescribing policy are given in which a safety-first approach is adopted. Propionic acid derivatives or newer drugs with similar properties are proposed as initial therapy to control symptoms of rheumatoid arthritis or osteoarthrosis. Special problems such as compliance in chronic disease, treatment of ulcer patients with anti-rheumatic drugs, therapy in the elderly, treatment for juvenile rheumatoid arthritis and treatment during pregnancy are considered. | |
| 6302824 | [Strict correlation between anti-RANA antibodies and anti-EBNA antibodies apart from rheum | 1983 Jan | In order to study the relationship between anti-RANA antibodies (aRANA) and the antibodies capable of recognising other Epstein-Barr induced antigens, we examined the sera of 51 patients without rheumatoid arthritis for anti-RANA, anti-EA, anti-VCA, and anti-EBNA antibodies. These subjects were cases of Burkitt's lymphoma, infectious mononucleosis, naso-pharyngeal carcinoma, immune disorders, Hodgkin's disease and normal controls. A blind study was conducted in two separate laboratories. Anti-RANA was detected by indirect immunofluorescence on RAJI cells synchronised in phase G1. There was a very strict correlation between anti-RANA and anti-EBNA with no correlation between anti-RANA and anti-EA or anti-VCA. In another experiment, 15 coded sera from patients with classical rheumatoid arthritis were tested following adsorption of the rheumatoid factor. One serum was found to be devoid of both anti-RANA and anti-EBNA. These results demonstrate that anti-RANA is widely distributed outside of rheumatoid arthritis and they question the distinction between EBNA and RANA, as the antibody titres directed against these antigens are regularly linked together. | |
| 6626437 | Pharmacokinetic observations on piroxicam in young adult, middle-aged and elderly patients | 1983 Oct | Nineteen out of 21 patients with painful conditions of the locomotor system aged between 27 and 94 years completed a study in which they received 20 mg piroxicam daily for 14 days. Plasma piroxicam concentrations were estimated by high performance liquid chromatography. Pharmacokinetic analysis of the data showed the half-life and systemic clearance of piroxicam to be unaffected by age. The apparent volume of distribution in older patients was higher than that of younger subjects. Multiple regression analysis showed that creatinine clearance and plasma albumin concentration had insignificant effects on the systemic piroxicam clearance. Although improvement in joint pain and stiffness occurred during the 2 week study period, this could not be correlated with plasma piroxicam concentration. | |
| 6979559 | A simple, rapid ELISA method for the detection of DNA antibodies. | 1982 May | Employing an enzyme-linked immunosorbent assay (ELISA) technique the serum antibodies against native (double stranded) and denatured (single stranded) deoxyribonucleic acid (DNA) have been measured in various disease groups and a group of blood donor sera. The ELISA method has been compared with a radioimmunoassay method using native (double stranded) DNA is substrate antigen and a latex-fixation technique using particles coated with soluble deoxyribonucleoprotein (SNP). It is concluded that ELISA offers an economic and reliable alternative to isotope techniques for the assessment of antibody content in systemic lupus erythematosus (SLE) and related disease states for the clinical laboratory. | |
| 7033187 | Cytochemical detection of proteases in synovial fluid of arthritic joints. | 1981 | A cytochemical method can be used to detect neutral proteases in polymorphonuclear leukocytes (PMN cells) in synovial fluid smears obtained from arthritic joints. Using this method, incubation of fixed smears in NaCl-borate buffer causes release of neutral PMN proteases, the enzymatic activity of which id documented in the degradation of exudate protein in ring-shaped areas centered around the neutrophils(halo effect). Such halo formation can also be detected around certain large mononuclear cells, as well as around very small particles - which may represent thrombocytes. Application of trypsin inhibitors and various antirheumatic agents causes concentration-dependent inhibition of halo formation. | |
| 6183906 | Pemphigus erythematosus induced by thiopronine. | 1982 | A patient affected by rheumatoid arthritis developed pemphigus erythematosus after 14 months of treatment with thiopronine. The lesions healed spontaneously after 4 weeks' withdrawal of the drug. Whereas the pemphigus erythematosus induced by D-penicillamine is a quite rare but well documented side effect, we believe our case to be the first one reported as being due to thiopronine. It is very interesting to note that the two drugs are very similar with regard to chemical structure, mechanism of action, therapeutic indications and also side effects. | |
| 6189492 | Anti-proliferative properties of Clozic, a disease-modifying anti-arthritic agent. | 1983 Feb 1 | Cytostatic drugs have been used in the treatment of rheumatoid arthritis but are of limited clinical application due to their severe toxic side-effects. We have discovered that 'Clozic' (ICI 55897), an agent with disease-modifying properties in rheumatoid arthritis patients, inhibits the growth of a variety of mammalian cell types including a matrix-secreting cell culture derived from neonatal rat hearts. The inhibition of growth was reversible and no loss of cell viability occurred when measured by lactate dehydrogenase released into the medium or by vital staining, suggesting a cytostatic rather than a cytotoxic mechanism. Cytostatic activity was observed at ICI 55897 concentrations within the reported therapeutic plasma concentration range and was related to the concentration of unbound compound, since the effect could be reduced by increasing the albumin concentration in the medium. Other oxyalkanoic acids inhibited cell growth. Their inhibitory potency correlated with lipophilicity. The anti-proliferative potencies of R and S enantiomers of two oxyalkanoic acids containing asymmetric centres were similar. These observations suggest that the anti-proliferative effect of the oxyalkanoic acids is due to their interaction with lipophilic cell target sites. | |
| 6285243 | [Radionuclide therapy of malignant and benign extra-thyroid diseases]. | 1982 Apr | Basis, indications and results of the endolymphatic therapy with radionuclides, of the selective therapy with radiophosphorus and radiostrontium and of the intraarticular and endocavitary therapy with radiocolloids are described. Future scientific engagement and intensive basic research in radionuclide therapy is required. However, because of the expected therapeutic profit the efforts seem to be justified. | |
| 6382112 | Vascularized muscular and musculocutaneous flaps for management of osteomyelitis. | 1984 Jul | Thirty-three cases of recalcitrant osteomyelitis treated by free tissue transfer are reviewed. The immediate success rate was 76 per cent; however, on follow-up for at least 1 year, there was a 12 per cent rate of recurrent drainage. The authors conclude that this is a good operation for this problem but that it is not a panacea because if followed long enough, a significant number of patients will develop recurrent drainage. | |
| 679527 | The results of Charnley arthroplasty of hip performed for protrusio acetabuli. | 1978 May | The results of total hip replacement in protrusio acetabuli using the Charnley technique have been very satisfactory. The immediate and late complications have been in no way different from those in ordinary cases of osteoarthritis and rheumatoid arthritis as performed in this hospital. There was no difference in the successful results whether a bone graft or cement alone had been used, and nothing to suggest that wire mesh need be used even in the severe cases. The diagnosis of idiopathic protrusio is 4 times more frequent than that of secondary protrusio. There was an incidence of 10 females for every male suffering from protrusio acetabuli. About half of the patients required bilateral low friction arthroplasties. | |
| 1136557 | [Allergic cobalt reaction (metallosis) following knee arthroplasty with vitallium endopros | 1975 Apr | A case report is given on a woman with rheumatoid arthritis and deformed knee joints. She got a knee arthroplasty with a metal-to-metal Vitallium endoprosthesis ad modum Walldius. About one year after the arthroplasty she showed both a sterile fistulation from the knee joint and an eczema of the skin of the operated knee. An epicutaneous test for cobalt was positive. It is suggested that the metal-to-metal contact in the prosthesis and the proximity of the prosthesis to the skin is a possible explanation to the allergic reaction. | |
| 1095021 | Lymphokines in the rheumatoid joint. | 1975 May | Joint fluids and culture supernatants of synovial tissue were examined for the presence of two lymphokines. Migration inhibitory activity was found in 16 of 22 rheumatoid fluids (73%), in 3 of 15 osteoarthritis fluids (20%), and in 3 of 11 fluids from patients with various inflammatory arthritides (27%). Blastogenic activity was present in 14 of 15 rheumatoid fluids and in 1 of 7 nonrheumatoid effusions. The active materials eluted with the third peak from Sephadex G-200 and were therefore smaller than immunoglobulin or immune complexes. These findings suggest that lymphokine-like substances were present in effusions and synovial tissue of most patients with rheumatoid arthritis and some patients with other forms of chronic synovitis. | |
| 6972766 | Low molecular weight IgM in rheumatoid arthritis and other rheumatic diseases. | 1981 Jun | Low molecular weight (LMW) IgM was measured in the serum and synovial fluid of patients with rheumatoid arthritis (RA) and other rheumatic diseases. High levels were seen in RA, particularly in rheumatoid vasculitis and Felty's syndrome, and significant correlations occurred between LMW IgM and the rheumatoid factor (RF) level and other indices that reflected active or severe disease. LMW IgM-RF, measured by radioimmunoassay in those column fractions containing LMW IgM, correlated significantly with LMW IgM (P less than 0.005); preliminary experiments suggested that in some sera, a considerable proportion of the LMW IgM consisted of LMW IgM-RF. We conclude that LMW IgM and LMW IgM-RF may have important implications in the immunopathogenesis of RA and other rheumatic diseases. | |
| 6634274 | The hypermobility syndrome. | 1983 Nov | The hypermobility syndrome has been recognized as a definitive diagnostic entity among children referred to a Pediatric Arthritis Clinic with musculoskeletal complaints. The diagnosis of hypermobility was made by the ability of the patients to perform at least three of the following maneuvers: (1) extension of the wrists and metacarpal phalanges so that the fingers are parallel to the dorsum of the forearm; (2) passive apposition of thumbs to the flexor aspect of the forearm; (3) hyperextension of elbows (greater than or equal to 10 degrees); (4) hyperextension of knees (greater than or equal to 10 degrees); (5) flexion of trunk with knees extended so palms rest on the floor. Of 262 patients, 15 (5.7%) referred to an arthritis clinic between January 1979 and July 1981 were subsequently determined to have hypermobility as the basis for their rheumatic complaints. Three of these 15 had concomitant juvenile arthritis. The presenting complaint of pain was most often localized to the knees, hands, and fingers. Arthritis and elevated ESRs were not seen except in the three patients who had concomitant juvenile arthritis. All patients responded to physical therapy and nonsteroidal analgesic anti-inflammatory drugs. This is an entity not sufficiently well recognized as a source of musculoskeletal complaints in the United States. It will usually respond well to reassurance and symptomatic therapy. | |
| 3885062 | IgG, IgA and IgM rheumatoid factors in patients with glomerulonephritis. | 1985 | Rheumatoid factors (RF), autoantibodies to IgG, have been postulated to have some pathogenetic role in the development of some types of glomerulonephritis. A simple and sensitive solid-phase fluorescence immunoassay was employed to determine whether IgG, IgA and IgM RF were detectable in sera from patients with various types of glomerulonephritis, rheumatoid arthritis (RA) and those with various streptococcal infections. IgG, IgA and IgM RF were significantly increased in the majority of patients with RA, lupus nephritis (SLE), acute poststreptococcal glomerulonephritis (APSGN) and various streptococcal infections. The titers of IgG and IgA RF were significantly higher in patients with APSGN than in those with simple pharyngitis. IgM RF was increated in patients with IgA nephropathy (IgA-N) and in those with membranoproliferative glomerulonephritis type I (MPGN). No significantly high RF was observed in membranous nephropathy (MN) or chronic mesangial proliferative glomerulonephritis without IgA deposition (PGN). It is suggested that some autologous immune mechanisms may be involved in the pathogenesis of some types of glomerulonephritis. | |
| 216090 | [Selective IgA deficiency, DPH-treated epilepsy and polyarthritis (author's transl)]. | 1978 Oct 8 | A selected IgA deficiency was found in a 66 years old, DPH-treated epileptic female, suffering from polyarthritis. Familial survey brings information for genetic transmission of IgA decreased concentrations in her offspring, unrelated with HLA A, B, C system. | |
| 4602134 | Circulating antinuclear antibody and rheumatoid factor in coal pneumoconiosis. | 1974 Jul 20 | Circulating antinuclear antibody and rheumatoid factor have been measured in 109 coal miners with pneumoconiosis whose chest radiograph showed a range of abnormalities varying from simple pneumoconiosis of mild degree to advanced progressive massive fibrosis.At a screening dilution of 1/10 the overall incidence of antinuclear antibody was 17%. In almost half of the positive cases the titre was 1/40 or greater.The prevalence of antinuclear antibody was lowest in those with simple pneumoconiosis (9%) and highest in those with category C progressive massive fibrosis (27%). A similar but less striking trend was seen with rheumatoid factor, ranging from 6% in simple pneumoconiosis to 18% in category C progressive massive fibrosis. The trend of increasing frequency of autoantibodies with advancing radiographic category was most marked when the frequencies of antinuclear antibody and rheumatoid factor were combined. These autoantibodies were found in 13% of the miners with simple pneumoconiosis and 45% of those with category C progressive massive fibrosis (P for the trend=0.01). |