Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 7025175 | How frequently should anti-inflammatory drugs be given? A study with indoprofen. | 1981 Aug | Indoprofen, despite its relatively short plasma half-life, was just as effective given twice daily as when the same daily total was given in four divided doses. There was a trend in favour of the twice daily regime for changes in morning pain and the duration of morning stiffness. Preferences were equally divided between the two regimes and efficacy was the usual reason for patients preferring one or other. Side-effects were no more frequent with the twice daily regime. Pharmacokinetics are no substitute for clinical experiment in planning the dosage regime of a non-steroidal anti-inflammatory drug. | |
| 4038355 | Synthesis, secretion, and deposition of fibronectin in cultured human synovium. | 1985 Sep | We examined fibronectin synthesis, secretion, and deposition in vitro by primary explants of rheumatoid synovium. Primary cultures initiated from tissue with monocytic infiltrates had higher levels of fibronectin synthesis; addition of dexamethasone at concentrations known to stimulate other tissue fibroblasts increased fibronectin synthesis and secretion. Newly synthesized fibronectin recovered from primary rheumatoid culture medium had a higher apparent molecular weight (240-245 kd), on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, compared with fibronectin recovered from passaged normal and rheumatoid cultures (230 kd). Primary rheumatoid explant cultures had a characteristic morphology which correlated with fibronectin deposition. Dense deposits of fibronectin extracellular matrix covered overlapping synoviocytes adjacent to esterase-positive mononuclear cells. Dexamethasone-treated cultures showed little fibronectin deposited as extracellular matrix and did not develop overlapping cellular networks. Characteristic patterns of fibronectin synthesis and deposition in primary rheumatoid cultures appear to result from interaction between fibroblastic and monocytic cells. This culture system may provide a model by which to study interactions between cells and extracellular matrix components that regulate synovial cell function. | |
| 6314493 | Scanning immuno-electron microscopy of a monoclonal, Epstein-Barr Virus(EBV) transformed h | 1983 | Selected lymphocytes from a patient with rheumatoid arthritis were infected with Epstein-Barr virus (EBV) and the emerging cell line (RF-AN) has now been successfully maintained in culture for more than two years. Multiparameter studies, including surface markers, ultra-structure and scanning immuno-electron microscopy, were utilized to characterize and evaluate this unique human cell line. Like other B-lymphoblastoid cell lines, RF-AN cells display multiple microvilli, contain surface and intracytoplasmic immunoglobulin (IgM lambda) and continuously produce monoclonal IgM lambda in vitro. The latter serves as an autoimmune antibody directed against IgG and is termed rheumatoid factor (RF). When RF-AN cells were incubated with human or rabbit IgG conjugated to polystyrene (latex) microspheres, almost all cells were labeled with the marker. However, cells did not label with goat IgG conjugated to latex. RF-AN cells were also labeled with latex microspheres coupled to goat antisera directed against human IgM or lambda chains but not with goat anti-human IgG or anti-human kappa chains. It is suggested that this mode of immunomicroscopy is reliable and may provide more useful data than other standard immunological techniques. | |
| 209184 | Gold-induced thrombocytopenia. | 1978 Summer | Ten patients with rheumatoid arhritis treated with gold sodium thiomalate developed thrombocytopenia without bone marrow asplasia. There was no life-threatening blood loss, but petechiae, purpura, or echymoses were seen in eight patients. The serum gold levels monitored in one patient did not exceed levels seen in patients without thrombocytopenia. In three cases peripheral blood lymphocytes were cultured in the presence of gold and tritiated thymidine incorporation was significantly increased. Eight patients responded to steroid therapy, one patient to BAL and pencillamine, and one patient to vincristine. | |
| 303237 | Pharmacokinetic studies of indoprofen in healthy volunteers and in patients. | 1977 Sep | The pharmacokinetics of indoprofen in healthy subjects after single oral and i.v. administrations is reviewed. During repeated administration of indoprofen to 6 normal subjects (200-mg tablet every 8 hours for 6 days) no variations in the disposition of the drug were found in comparison with single dose administration. In 6 inpatients, with rheumatoid arthritis, the pharmacokinetics of indoprofen was studied after single oral (tablet) and i.m. administration. As for oral doses, no difference in main kinetic parameters was detected between the patients and normal subjects except for a higher volume of distribution in the former population. The bioavailability of the drug given by i.m. injection was not significantly different from that observed after oral administration. | |
| 391529 | Flurbiprofen: a review of its pharmacological properties and therapeutic use in rheumatic | 1979 Dec | Flurbiprofen, a phenylalkanoic acid derivative, is a non-steroidal anti-inflammatory, antipyretic, analgesic agent advocated for use in rheumatoid arthritis, degenerative joint disease, ankylosing spondylitis and allied conditions. Published data suggest that flurbiprofen 120 to 150 mg daily is comparable in effectiveness with therapeutic doses of aspirin (3 to 4 g) in rheumatoid arthritis, but generally causes fewer side effects. Flurbiprofen 150 to 300 mg appears to be comparable with 75 to 150 mg of indomethacin in rheumatoid arthritis and degenerative joint disease, and comparable with phenylbutazone or indomethacin in ankylosing spondylitis. In comparison with other non-steroidal agents, flurbiprofen appears to be at least as effective as naproxen, ibuprofen or sulindac, but generally causes more side effects than these drugs. However, as no one of the non-steroidal anti-inflammatory agents is the most suitable drug for all patients requiring such therapy, flurbiprofen should be considered along with other drugs of its type in the arthritic patient. | |
| 4053441 | Specificity of antibodies in rheumatoid arthritis. I. A controlled study of humoral antibo | 1985 Jul | Rheumatoid arthritis (RA) sera were compared in a matched, controlled study to non-RA sera for their ability to react in ELISA with antigen preparations extracted from bovine nasal cartilage. Antibodies to matrix proteins, proteoglycans and whole extract were significantly higher in RA sera than in non-RA with the reactivity for matrix proteins giving the largest difference. There was no significant difference between RA and non-RA antibody levels for collagen and collagen alpha chains. By SDS-PAGE, large pore composite gel electrophoresis, and uronic acid analysis, the matrix protein fraction contained 8 major proteins as well as two electrophoretic species of low density proteoglycans distinct from the major high density cartilage proteoglycans. Further fractionation provided a proteoglycan-free preparation containing six major proteins of 66-13 kd with which the RA sera were still highly reactive. | |
| 86201 | Demonstration of electrophoretic heterogeneity of serum beta 2-microglobulin in systemic l | 1979 Mar | A sensitive crossed radioimmunoelectrophoretic method (CRIE), originally developed to study lymphocyte-associated beta 2-microglobulin (beta 2m), was applied in the study of serum beta 2m in patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). In six of seven patients with SLE and nineteen of twenty-seven patients with RA a considerable electrophoretic heterogeneity of serum beta 2m was found. In addition to the normally seen symmetric beta 2m precipitate, a beta 2m precipitate exhibiting complete immunochemical identity was found in the alpha-electrophoretic region. Binding of isolated 125I-labelled beta 2m to the abnormal precipitate was demonstrated in crossed immunoelectrophoresis. After gel filtration of sera exhibiting the above-mentioned beta 2m binding, all beta 2m was eluted in low molecular weight fractions corresponding to free beta 2m. By application of appropriate antisera and a glycoprotein-binding lectin in intermediate gels in CRIE, it was shown that the possible beta 2m-binding ligand is not an antibody, not a major constituent of normal human serum, and not unmodified HLA alloantigen. The abnormality was not restricted to patients with high disease activity but was found more frequently and was more pronounced (mean score 1.6 arbitrary units against 0.57 arbitrary units, P less than 0.01) in such patients. Thus our data exclude the possibility that autoantibodies to beta 2m were present in serum from patients with SLE and RA. | |
| 6652387 | Phototoxic reactions to piroxicam, naproxen and tiaprofenic acid. | 1983 Nov | Photosensitivity investigations have been carried out using an irradiation monochromator on 31 patients taking one of seven different nonsteroidal anti-inflammatory agents for the treatment of rheumatoid and osteoarthritis. Six patients, who were taking either piroxicam, naproxen or tiaprofenic acid, experienced adverse immediate reactions of erythema and flaring, together with an urticarial response in four of these six patients. No phototoxic response was observed in patients taking either indomethacin, ketoprofen, benorylate or ibuprofen, although firm conclusions about the non-phototoxic nature of these four drugs cannot be drawn from this pilot study because of the small numbers of patients investigated. | |
| 532371 | [Pathomorphosis of the stratum synoviale]. | 1979 Nov | The stratum synoviale is a sort of central point in the synovial system. That is the reason why we also find corresponding modifications in the stratum synoviale in most of the joint diseases. They will be discussed in the appropriate shortness, summarized in tables and their histological appearance form will also be partly represented. | |
| 7224742 | Rheumatoid nodulosis. A relatively benign rheumatoid variant. | 1981 Apr | Subcutaneous nodules and rheumatoid factor (RF) are criteria used to diagnose rheumatoid disease. Their presence correlates with disease severity and poorer prognosis. They have been reported, however, in patients with little arthritis and no systemic disease. We studied four such patients, in whom (1) RF was present in high titer; (2) nodules were often extensive (nodulosis) and involved elbows, hands, and feet, with a predilection for tendons; and (3) roentgenograms showed large, subchondral bone cysts without cortical erosion of correlation with nodule location. The conditions of three of these patients had been previously misdiagnosed as gout or xanthoma. Our findings were similar to those in seven other patients described in earlier reports. We suggest that nodulosis, bone cysts, and elevated RF with little active arthritis constitute a relatively benign variant of rheumatoid disease. | |
| 7449526 | [Tissue typing of blood lymphocytes in normal Chinese and diseases (author's transl)]. | 1980 Mar | The histocompatibility antigens were determined in 170 normal Chinese by a modified micro-lymphocytotoxicity test of Terasaki using 26 typing sera obtained from Behring Laboratories and Stanford University, and the data were compared with those obtained from 36 systemic lupus erythematosus, 30 rheumatoid arthritis, 17 ankylosing spondylitis as well as 45 leprosy patients. In normal individuals HLA-A2,A11 and A9 were dominant in locus A, the frequency were 42.35%, 41.76% and 32.35% respectively. HLA-Bw17, B13 and B5 were dominant in locus B, the frequency were 55.29%, 19.41% and 14.70% respectively. In systemic lupus erythematosus, the frequency of B8, Bw38 and A3 were slightly higher than normal (relative risk > 2); the frequency of Bw21 and B7 were little lower (risk of Bw21 < 0.5, frequency of B7 > 5% in normals but none in patients). In rheumatoid arthritis, the frequency of A28 and A10 (Aw25+26) were slightly lower than normal (risk < 0.5). In ankylosing spondylitis, the frequency of B27 was extremely high (risk = 44.92), Aw24 was also rather high (risk > 2); the frequency of B5, Bw35 and A10 (Aw25+26) was low (risk < 0.5), Bw15 and Bw21 > 5% in normals but none in patients. In leprosy, the frequency of B18 was relatively high (risk > 2); A3, Aw30+31+32, B27 and Bw35 were somewhat low (risk < 0.5). Because of the small sample size, however, the differences were not significant by Chi square analysis except the high frequency of B27 in ankylosing spondylitis (corrected P < 0.001). | |
| 6983117 | In situ characterization of mononuclear cells in rheumatoid tissues, using monoclonal anti | 1982 Oct | The reactivity of monoclonal mouse anti-human antibodies specific for mononuclear cell surface antigens were studied by the indirect immunofluorescence technique in frozen synovial tissue sections from patients with rheumatoid arthritis (RA). Most of the proliferating synovial lining cells were positive for HLA-DR antigens and monocyte-specific antigens, since they reacted with the OKIa1 and OKM1. Cells positive for HLA-DR and monocyte antigens were also seen scattered or in small nests in the synovial stroma, probably representing synovial cells or monocytes/macrophages. Some of the HLA-DR-positive cells may also be B lymphocytes or activated T cells. Endothelial cells were also HLA-DR antigen-positive. Monoclonal antibodies with specificity for all T cells (OKT3), for helper/inducer cells (OKT4), and for suppressor/cytotoxic cells (OKT8) reacted with cells often located in follicle-like structures around vessels. Cells with the T4 phenotype tended to be located in the centre of the follicles, whereas the T8 positive cells were more peripherally situated. In most instances fewer cells were positive for the T8 than for the T4 marker. In some instances there was as many T8-positive cells as T4-positive cells. Complete lack of T-lymphocyte subpopulations was not seen. | |
| 6212555 | Comparative efficacy of etodolac and placebo in rheumatoid arthritic patients. | 1982 May | Twenty-four patients with active rheumatoid arthritis were studied in a 4-week double-blind, placebo-controlled, parallel group trial. They were treated with a low dose (25, 50, and 100 mg twice daily) or high dose (100, 200, or 300 mg twice daily) of etodolac or with placebo. In both groups four patients received placebo and eight the active drug in a fixed-titration regimen. Doses were increased weekly and kept at the highest level during the last 2 weeks. Clinical and laboratory assessments were completed before drug and on days 8, 15, and 29. Seventeen patients completed 29 days and seven discontinued the study earlier: six on placebo and one on low dose. Etodolac-low dose was significantly more effective than placebo in nine of ten clinical assessments and in all ten at high dose. Etodolac was well tolerated. All patients had negative tests for occult blood at all times. Etodolac was an effective anti-inflammatory agent and appeared to be safe in doses of 50-600 mg per day. | |
| 6572296 | [Our experience with the effectiveness and tolerance of ibuproxam in rheumatology]. | 1983 Feb 25 | The tolerance and effectiveness of Ibuproxam were investigated in 54 patients with arthrosis, R.A., ankylosing spondylitis, and fibrositis. Statistically significant reduction of pain at the end of the treatment was accompanied by decreased limitation of joint movement and morning stiffness. Simultaneous investigation of clinical and biological tolerance showed that the drug is infrequently responsible for side-effects that are always of negligible importance. Oesophagogastroduodenoscopy in subjects with a prior history and/or signs of active pathology of the upper digestive tract did not reveal exacerbation or recrudescence of lesions, nor the appearance of acute lesions. | |
| 7438618 | Hematogenous infection of total joint implants: a report of multiple joint infections in t | 1980 Oct | Three patients had hematogenous infections caused by the same organism (as determined by identical antibiotic sensitivity profiles) in more than one joint. In each of the patients, the same infecting organism had also been recovered from a previous distant infection. Evidence that sepsis resulted from late hematogenous contamination is overwhelming. All three cases clearly had at least some late total joint infection which was not from organisms introduced at the time of total hip arthroplasty but from hematogenous contamination from a distant site occurring at a late date unknown to the surgeon. Patients with a total joint arthroplasty should probably have systemic antibiotic coverage to protect the prosthetic joint against hematogenous contamination from distant sites of infection and from potential bacteremia induced during medical or dental procedures. | |
| 160073 | Out-patient physiotherapy: patterns of provision. | 1979 Nov | A total of 1014 physiotherapy out-patients and their therapists were interviewed at 10 hospitals in Oxfordshire and Devonshire, including a District General Hospital, a Geriatric, and a sample of associated Community Hospitals in each of the two regions. Over 70% of these patients were suffering from long-term disabilities. The proportion of this type of patient varied between the hospital types, and this variation was similar in the two regions. The overall frequencies with which the different physiotherapy treatments were employed were, for the most part, similar in all departments regardless of hospital type or regions involved. Exercises and heat were the predominant treatments everywhere. The standard frequency of attendance was twice or three times a week. One third of the patients used hospital transport; most patients attended a hospital reasonably close to their homes. | |
| 522026 | [Radiological opacities after intra-articular injection of osmic acid. Relationship with t | 1979 Oct | Abnormal radiological opacities are sometimes observed after intra-articular injection of osmic acid. These opacities are radio-opaque because osmium is a heavy metal (atomic number = 76). They are usually found near the suprapatellar pouch which is the usual injection site. A parasynovial injection (or back flow from the joint cavity) of some of the osmic solution, followed by concentration and fixation of the osmic deposits at this level, seems to be the cause of these radiological opacities. This could be the reason for the poor clinical results encountered in some cases of osmic acid therapy followed by such deposits. | |
| 887037 | Aspirin, phenacetin, and the kidney: a rheumatism clinic study. | 1977 Jun 25 | In a rheumatism clinic study in Brisbane, the consumption of aspirin and phenacetin compounds (APC) containing more than one kilogram of aspirin and phenacetin was significantly associated with overt renal papillary necrosis (RPN) and with a renal score, but when more than one kilogram of aspirin was taken without phenacetin there was no such association. Analysis and comparison with the parallel New Zealand study showed an overall risk of RPN of at least 10-6% when more than one kilogram of aspirin was taken in APC form, and 0-3% when aspirin was taken without phenacetin. An additive interaction of phenacetin and aspirin is postulated. It is concluded that phenacetin use should be severely restricted. | |
| 123703 | The clinical response of 328 private patients to acupuncture therapy. | 1975 Apr | It is the attempt of this study to determine the efficacy of response to acupuncture at 24 hours, one week, and three week intervals at the end of a series of treatments in 328 patients. Treated for a variety of disorders, approximately 60% had satisfactory responses of up to 3 weeks duration. The 328 patients were classified into 13 categories of problem disorders: headaches, cervical pain, lumbar pain without radiation, lumbar pain with radiation, isolated sciatica, parathoracic pain, knee pain, elbow pain, shoulder pain, gereralized musculoskeletal pains (rheumatism), neurological disorders, and a general miscellaneous category. The problem disorders were then arranged into etiological categories. In general for each problem disorder, except for neurological, the average response ranged in the 60-65% range at the third week interval. |