Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 2491398 | [Anti-RANA antibodies and rheumatoid arthritis]. | 1989 | Anti RANA antibodies were determined by the method of indirect immunofluorescence in 51 patients with the classic resp. definitive form of rheumatoid arthritis (ARA) and in 19 healthy persons. Anti RANA antibodies were found in 92% of the patients with rheumatoid arthritis and in 16% of the control group. The reactive nuclear antigen is present in human B lymphocyte tissue cell cultures transformed by the EPSTEIN-BARR virus--Raji cells. The anti RANA antibody test is very suitable for use in the diagnosis of rheumatoid arthritis. | |
| 3415629 | The role of monocytes in rheumatoid synovitis. | 1988 | In non-specific and rheumatoid synovitis, the use of specific monoclonal antibodies against antigenic determinants of cells of the immune system showed that the characteristic changes of rheumatoid synovitis are located in the synovial internal layers. The monocytes were OKM1, OKM5, S100, OKDR positive, while the subintimal monocytes in non-specific synovitis were OKDR negative. We suggest that, in rheumatoid synovitis, the previously activated monocytes are transported by the bloodstream and pass through the so-called "sinovial barrier" to arrive in the subintimal layers ready to interact with T helper lymphocytes and initiate the immune response mechanisms responsible for lesions in rheumatoid synovitis. | |
| 3712050 | Indium-111 leukocyte imaging in patients with rheumatoid arthritis. | 1986 Mar | This study evaluates the usefulness of labeled leukocyte imaging in patients with rheumatoid arthritis. In 33 patients, the incidence of pain and swelling in 66 wrist joints and 66 knee joints was compared with the accumulation of [111In]leukocytes. No accumulation of [111In]leukocytes was seen in any of the patients' wrists (0/12) or knee joints (0/14) when both pain and swelling were absent. In contrast, 93% (25/27) of wrist joints and 80% (24/30) of knee joints with both pain and swelling were positive by [111In]leukocyte scintigraphy. There was little correlation between the stage of the disease, as determined by radiography, and [111In]leukocyte accumulation. This study suggests that [111In]leukocyte imaging may be a reliable procedure for monitoring the activity of rheumatoid arthritis, especially for confirming the lack of an ongoing inflammatory response. | |
| 3246133 | Quantitative computed tomography of the rheumatic knee. | 1988 Dec | A good noninvasive means to quantitate the extent and course of bone loss in rheumatoid arthritis (RA) is currently not available. We report on ways of assessing arthritic bone changes by means of quantitative computed tomography (QCT). The visualization of lesions and the quantitation of bone density diminutions are based on a stack of forty thin computed tomograms and 3D reconstructions. A pilot study shows that QCT may be a useful tool in the noninvasive assessment of the progression of RA and that it may help to evaluate the efficacy of therapeutic procedures. | |
| 2217041 | Spontaneous rheumatoid fistula of the elbow joint. | 1990 Aug | A case of rheumatoid arthritis, whose presenting problem was a non-infective fistula of the elbow joint, is described. Penicillamine therapy was ineffective, but synovectomy led to healing. | |
| 1895263 | Response to glucocorticoid treatment in rheumatoid arthritis: in vitro cell mediated immun | 1991 Jun | Variable treatment responses to glucocorticoids occur in patients with rheumatoid arthritis (RA). In renal transplantation and asthma treatment responses correlate with in vitro tests of glucocorticoid immunosuppression. We compared in vitro methylprednisolone suppression of concanavalin A stimulated cellular proliferation with clinical responses to methylprednisolone in patients with RA. Patients found to be glucocorticoid sensitive by in vitro testing had significantly greater improvements in joint score and soluble interleukin 2 receptor (sIL-2R) levels compared to control patients, indicating that individual responsiveness to glucocorticoid exists in RA. Similar in vitro and in vivo changes of sIL-2R levels suggests that they reflect cell mediated immune events in vivo. | |
| 3132576 | Stiffness of the rib cage in a subset of rheumatoid patients. | 1988 | In a previous investigation of lung function in patients with rheumatoid arthritis (RA), we observed that some patients had significant dysfunction of their chest wall mechanics without pleural disease that could contribute to their respiratory symptoms. To investigate further the chest wall functions of patients with RA, we selected 10 female lifetime nonsmoking patients with RA and compared them to 10 paid normal volunteers in detailed functional evaluations of lung and chest wall. Patients with significant airflow limitation were excluded from this study. We found a significant 7% reduction in total lung capacity in the RA patients without significant change in lung compliance. However, we found a significant reduction in rib cage compliance in patients with RA (0.044 +/- 0.006 L/cmH2O vs. 0.064 +/- 0.012 in controls), which was associated with a significant 38% reduction in expansion of the rib cage volume during various breathing maneuvers. These changes occurred in the absence of lung or pleural disease. This study documents that patients with RA have limited expansion of rib cage relative to abdomen, thus rib cage restriction and stiffness, which may contribute to reduced lung volume. The shift of thoracoabdominal breathing configuration observed in patients with RA will likely reduce the effectiveness of breathing muscles and thus could contribute to the dyspnea sensation experienced by patients with RA. | |
| 3401051 | Class-specific rheumatoid factors, DR antigens, and amyloidosis in patients with rheumatoi | 1988 Jul | Class-specific rheumatoid factors (RFs) were measured by enzyme immunoassay in 59 patients with rheumatoid arthritis complicated by systemic amyloidosis (RA+A), 47 patients with rheumatoid arthritis without amyloid (RA), 106 patients with other rheumatic diseases (juvenile rheumatoid arthritis, systemic lupus erythematosus, Sjögren's syndrome), and 55 blood donors. The patients with RA+A were characterised by a high prevalence of RF negativity; the IgM RF concentration was raised in only 18 of the 59 patients (31%, p less than 0.001 v RA), the IgG RF concentration in 20 of 59 (34%, p less than 0.001 v RA), and the IgA RF concentration in 24 of 59 (41%, p less than 0.001 v RA). A higher prevalence of HLA-DR4 (p less than 0.001) and a lower prevalence of DR2 (p less than 0.05) were found among 48 tested patients with RA+A when compared with a control panel consisting of 500 blood donors. No significant differences in the prevalence of DR1-DR7 or B27 antigens were observed, however, between patients with RA with or without amyloid. | |
| 2257452 | The cycling of combination antirheumatic drug therapy in rheumatoid arthritis. | 1990 Dec | In this open pilot study a combination of hydroxychloroquine, prednisolone and alternating months of treatment with sulphasalazine or oral weekly pulse methotrexate has been investigated in 16 patients with rheumatoid arthritis (RA) refractory to a total of 67 disease suppressive medications. Results at 3 months indicated significant improvements in visual analogue score for pain, joint count, Ritchie index, scale of disability related to activities of daily living, ESR, rheumatoid factor and C-reactive protein. This degree of improvement, however, was not maintained 6 and 12 months after commencement of treatment. Pain score, Ritchie index and ESR were the only parameters demonstrating significant improvement at 12 months. Therapy was terminated in eight patients, half due to lack of efficacy and half because of side effects. | |
| 1749945 | A double-blind comparison of parenteral methotrexate and parenteral gold in the treatment | 1991 Oct | One hundred two patients with active erosive rheumatoid arthritis (RA) without malalignment or deformities (median disease duration, 14 months) entered a double-blind, randomized study to compare the effects of 50 mg gold sodium thiomalate (GST) with 15 mg methotrexate (MTX) administered intramuscularly for 12 months. Roentgenograms of hands, wrists, and forefeet were taken at baseline and after 6 and 12 months, and 32 joints were evaluated according to Larsen. Sixteen of 50 patients in the MTX group were withdrawn; one patient in the MTX group died of cerebral bleeding that was not related to treatment. Thirty-four GST patients and 44 MTX patients were evaluated for efficacy. Thirty-eight joints were counted. The number oftender and swollen joints, the Lansbury articular index, morning stiffness, activities of daily living (ADL) score, and erythrocyte sedimentation rate improved significantly in both groups without statistical intergroup differences. After 12 months, there was a significant deterioration of the mean Larsen index and the number of joints with erosions without intergroup difference. However, the radiological progression was retarded significantly during the second 6-month period in the gold group, whereas this effect was less pronounced in the MTX group. At 12 months, the progression rate was the same in both groups. | |
| 2970263 | Sex hormone status and osteoporosis in postmenopausal women with rheumatoid arthritis. | 1988 Aug | Sex hormones have important effects on bone, especially in postmenopausal women. These hormones may be of particular significance in patients with rheumatoid arthritis (RA), who have a high frequency of osteoporosis. To examine this, we measured estrogen and androgen concentrations and bone mineral density (BMD) in 49 postmenopausal women with RA and 49 normal postmenopausal women. Compared with the controls, postmenopausal RA patients had significantly reduced levels of estrone (median 18 pmoles/liter versus 49; P less than 0.001), dehydroepiandosterone sulfate (DHEAS) (median 0.3 mumoles/liter versus 2.0; P less than 0.001), testosterone (median 0.6 nmoles/liter versus 0.95; P less than 0.001), and femoral BMD (mean 0.72 gm/cm2 versus 0.80; P less than 0.002). Prednisolone therapy in 22 patients (mean dosage 8 mg/day) was associated with reductions in estrone and testosterone levels; however, DHEAS and femoral BMD were also decreased in RA patients who were not receiving corticosteroids. Reduced DHEAS levels in postmenopausal women with RA may increase their risk of osteoporosis. | |
| 2691148 | Mechanisms of bone and cartilage destruction in rheumatoid arthritis: lessons from the str | 1989 Sep | Rheumatoid arthritis is a proliferative and erosive disease which has been described as tumor-like. Streptococcal cell wall-induced arthritis in the LEW/N rat is also tumor-like and closely simulates the features of joint destruction that develop in rheumatoid arthritis. This article discusses the mechanisms of bone and cartilage destruction in streptococcal cell wall arthritis with particular emphasis on the tumor-like behavior of synovial connective tissue cells and the role of cytokines, such as platelet-derived growth factor, transforming growth factor beta and interleukin 1, in regulating this abnormal behavior. | |
| 2251507 | Proposition: methotrexate should not be the first second-line agent to be used in rheumato | 1990 Oct | Although methotrexate (MTX) is an effective antirheumatic drug, it cannot clearly be defined as a disease modifying antirheumatic drug (DMARD), when this term is characterized by its effect on radiographs or laboratory data. Current data, in the form of small studies or case reports, show that MTX's hepatic toxicity is not yet fully defined, that its acute pulmonary toxicity is significant, that systemic fungal infections may be associated with MTX use in rheumatoid arthritis (RA), that unexplained significant weight loss can be a problem, and that the consequence of drug interactions with MTX are not yet fully known. Thus, although clearly an effective antiinflammatory drug in RA, the place of MTX in the RA armamentarium is not fully defined. For this reason, MTX should not at present be used as the first second-line agent in RA after nonsteroidal antiinflammatory drugs (NSAIDs) fail. | |
| 3305938 | Matrix metalloproteinases 1, 2, and 3 from rheumatoid synovial cells are sufficient to des | 1987 May | A neutral metalloproteinase has been isolated and purified from adherent rheumatoid synovial cells in culture. This protease, named matrix metalloproteinase 3, (MMP-3) degrades gelatin, proteoglycan, fibronectin, type IV collagen, laminin, and the N propeptide of type I procollagen. It can be separated from MMP-2 (a potent gelatinase), and MMP-1, an interstitial collagenase. MMP-3 is released from cells as a proenzyme of 55 Kda. Activation by trypsin or organic mercurials produces 2 active species of 45 Kda and 28 Kda. The enzyme contains zinc as an intrinsic component and requires calcium for conformational stability. In concert, active MMP-1, -2, and -3 can destroy all significant structural proteins of joint structures. | |
| 3529373 | Antibodies to extractable nuclear antigens (ENA) in rheumatoid arthritis assayed by ELISA. | 1986 | A clinicopathological study of autoantibodies in the sera of 53 RA patients was performed. Antibodies to extractable nuclear antigens (ENA) were assayed by ELISA and were found in 42% of the subjects, all bound to RNAase sensitive ENA, and these antibodies were significantly associated with the presence of tendon nodules (p less than 0.05). Antibodies to dsDNA were found in 16%, and rheumatoid factor (RF) was present in 81%; neither of these antibody groups were associated with any of the clinical abnormalities examined for. Comparisons between anti-ENA, anti-dsDNA and anti-immunoglobulin autoantibody parameters in RA subjects revealed ENA and dsDNA antibody levels to be significantly mutually related (p less than 0.01) but both were independent of RF levels. We concluded that in RA, ENA antibodies constitute a unique autoantibody subset, that may result from an immune response to an autoantigen directly linked with the aetiopathogenesis of RA. | |
| 2312565 | The effect of a stem on the tibial component of knee arthroplasty. A roentgen stereophotog | 1990 Mar | We studied the effect of a metal tray with an intramedullary stem on the micromotion of the tibial component in total knee arthroplasty. Of 32 uncemented Freeman-Samuelson knee arthroplasties performed in London and Gothenburg, nine had a metal backing and stem added to the tibial component. Micromotion of the tibial components, expressed as migration and inducible displacement, was analysed using roentgen stereophotogrammetric analysis up to two years follow-up. The addition of a metal back and a 110 mm stem to the standard polyethylene component significantly reduced both migration over two years and inducible displacement. | |
| 3628760 | Rheumatoid arthritis of the cervical spine: early and progressive radiographic features. | 1987 Oct | One hundred thirty-three cervical spine radiographs of 50 patients with rheumatoid arthritis were examined. The patients were a representative random sample chosen from among 675 patients followed up for more than 10 years. The frequency of occurrence for each radiographic finding was correlated with the duration of rheumatoid arthritis. The six radiographic findings that occurred in 30% or more of patients with a disease duration of less than 10 years included odontoid erosions, subaxial subluxation, superficial apophyseal joint erosions, apophyseal joint sclerosis at levels C-4 through C-7, and diskovertebral joint narrowing and osteophytosis at C-4 through C-7. Familiarity with these early findings in the cervical spine may be helpful in both diagnosing and managing rheumatoid arthritis. Subaxial subluxation and superficial apophyseal joint erosions did not progressively increase in frequency with increasing duration of disease, but the other four features did. Other features that were uncommon in early disease became significantly more frequent in later decades. | |
| 2254883 | Clinical, laboratory and radiographic features in early rheumatoid arthritis. | 1990 Oct | We evaluated disease status in relation to age, sex and disease duration using some short term indices of disease activity, laboratory tests, and radiological features in 315 patients with rheumatoid arthritis of duration varying from 3 to 36 months (mean 12 months). No differences were observed among various age groups in disease duration, female/male ratio, incidence of radiologic lesions and other indices of disease process. Some clinical markers of the disease process such as involvement of the flexor tendons of the hands and Ritchie's index (score greater than 9) were significantly more frequent in the women (p less than 0.0013 and p less than 0.04, respectively). In the patients with disease of recent onset women were slightly more numerous (56%) than men; however, in those with disease duration of 36 months there were significantly more women (72%) (p less than 0.039), suggesting a greater tendency to chronic disease in this sex. Radiological lesions of the small joints of the hands, feet, and/or wrists were found in 37% of the cases with disease duration of up to 4 months and in 91% at 36 months (p less than 0.0001). The lesions were associated significantly more frequently with Ritchie index (p less than 0.02) and with laboratory indices of inflammatory activity (erythrocyte sedimentation rate greater than or equal to 25 mm/h) (p less than 0.001) and immune response (latex test greater than or equal to 80) (p less than 0.0001). Logistic regression analysis showed that the duration of illness is the most important factor correlating with radiologic lesions. | |
| 2200205 | [Proteolytic activity of blood cells in rheumatoid arthritis]. | 1990 May | Collagenolytic and neutral caseinolytic activities were studied in lysates of neutrophils, mononuclear cells and thrombocytes of patients with rheumatoid arthritis considering the steps and duration of the disease, before and after medical treatment. 5 healthy persons and 18 patients with rheumatoid arthritis were investigated. Specific caseinolytic activity (per 1 mg of protein) was increased in the patients neutrophils and monocytes 2.2- and 3.2-fold, respectively, while the activity per 10(6) cells was increased 1.5- and 2.2-fold, respectively. Specific collagenolytic activity was increased 1.4-fold in neutrophils and monocytes, whereas the activity per 10(6) neutrophils was increased by 20% and per 10(6) monocytes--near normal values. In thrombocytes collagenolytic activity was decreased by 40% and caseinolytic activity was similar to controls. Concentration of protein was decreased 1.5-fold in neutrophils and monocytes of the patients as compared with healthy persons. Proteinases are of importance in pathogenesis of rheumatoid arthritis and collagenolytic activity correlated most distinctly with development of the disease. After medical treatment collagenolytic activity was normalized in the patients cells studied, while caseinolytic activity was markedly decreased but remained above control values. Experiments with proteinase inhibitors enabled to detect major types of neutral proteinases in blood cells of patients with rheumatoid arthritis and to find some differences in the enzyme patterns in various blood cell populations. | |
| 3042079 | Antibodies to Proteus in rheumatoid arthritis. | 1988 | Increased levels of Proteus antibodies were found in patients with rheumatoid arthritis and coeliac disease, when compared to normal controls or patients with SLE and sarcoidosis. |