Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 1839528 | [Immunopathological study of synovium of rheumatoid arthritis]. | 1991 Aug | 46 patients with different rheumatic disorders were subjected to arthroscopic examination and the biopsied synovia were studied immunopathologically. Immunoglobulins, complements and fibrinogen deposition were detected by direct immunofluorescence, and rheumatoid factor (RF) deposition was detected by peroxidase-labelled denatured human IgG. Postoperative diagnosis of these cases were as follows: rheumatoid arthritis (RA) 21; seronegative spondylarthropathy 4; reactive arthritis 2; unidentified synovitis 4; osteoarthritis 8; and nonsynovitis conditions 5. It was shown that the positive rates of deposition of IgG, IgM, complement C3, C1q and C4, and RF in RA were 95.2%, 52.4%, 38.1%, 28.6%, 9.5% and 42.9%, respectively. Deposition was negative in all other rheumatic diseases. The positive fibrinogen deposition rate in RA was 42.9%; it was weakly positive in other disorders as well. The results indicate that the pathogenesis of RA is related to humoral immunity, and immunopathological study of the synovium has very important diagnostic significance in RA. On the other hand, deposition of fibrinogen lacks specificity for any rheumatic disease. The deposition of RF IgG, IgM and complement C3, C1q and C4 in the synovium of RA patients does not correlate well with clinical disease activity. The deposition of RF in the synovium also does not show paired correlation with blood IgM RF determination. This difference may be explained by the fact that the synovial RF examined in the present study may include all isotypes of RF and that hidden-RF in the blood may give a negative result. The synovium is the site of production of RF, but the degree of pathological change of the synovium of different joints in a single patient may not be uniform. The results of humoral immunity studies of the RA synovium may be influenced by the site selected for synovial biopsy. | |
| 2499551 | Intestinal permeability and antigen absorption in rheumatoid arthritis. Effects of acetyls | 1989 | Intestinal permeability was measured using cow's milk beta-lactoglobulin absorption (BLG) as a permeability marker in 14 patients with active and inactive rheumatoid arthritis (RA) under three different conditions: after a washout period, after treatment with acetylsalicylic acid (ASA) associated with disodium chromoglycate (DSCG), and with ASA only. No intolerance to cow's milk was present and serum IgE levels were in the normal range in 12 of 14 patients. IgG anti-IgE were present in 7 of 13 patients tested. When off treatment the intestinal permeability to BLG in RA patients was not increased as compared to controls, but we found a significative difference between active and inactive RA. ASA administration strongly increased BLG absorption, not prevented by DSCG pretreatment. In normal controls treated with a single dose of ASA we obtained similar results. Our results suggest that prolonged treatment with nonsteroidal anti-inflammatory drugs induces an increase of food antigen absorption, apparently not related to anaphylaxis mediator release, with possible clinical effects. | |
| 3418640 | The transsynovial lymphocytic ratio. Characterization of blood and synovial fluid lymphocy | 1988 Jun | Monoclonal antibodies and flow cytometry techniques were used to analyze and compare the distribution of lymphocyte subpopulations of peripheral blood and synovial fluid (SF) from 70 patients, 43 with rheumatoid arthritis (RA), 10 with ankylosing spondylitis (AS) or reactive synovitis, 10 with psoriatic arthritis and 7 with other inflammatory arthritic diseases. Patients with RA had significantly reduced number of CD8+ T cells and greater CD4/CD8 ratios in peripheral blood, a greater number of CD4+ T cells and lower CD4/CD8 ratio in SF. No significant difference was found between the groups with AS, reactive synovitis and psoriatic arthritis. The simultaneous analysis of peripheral blood and SF lymphocyte subpopulations allowed us to establish a transsynovial lymphocytic ratio which reflects CD4/CD8 variations on both peripheral blood and SF, 2 easily accessible compartments for physicians. This new ratio may distinguish RA from other inflammatory arthritic diseases. | |
| 1930329 | Phospholipase A2 is a major component of the salt-extractable pool of matrix proteins in a | 1991 Sep | Adult human articular cartilage contains a component with an apparent molecular weight of 16 kd, which is extractable with high ionic strength buffers. This protein, which, in addition to lysozyme, is one of the most prominent components in salt extracts of adult cartilage, is not detectable in cartilage from newborns. We performed N-terminal sequence analysis to identify the protein. The amino acid sequence obtained for the first 20 residues was identical to that reported for phospholipase A2 (PLA2) from human placenta and human synovial cells. The extractable PLA2 was found to be active. The lack of PLA2 in salt extracts from newborn cartilage observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis was confirmed by the very low levels of PLA2 activity detectable in these preparations. PLA2 was clearly present in cartilage extracts from an 18-year-old subject and a 19-year-old subject, suggesting that its accumulation begins at some stage during the adolescent growth period. The enzyme does not appear to be released from cartilage matrix under normal physiologic conditions, and it is possible that the accumulation of PLA2 in maturing cartilage is a result of the decreased matrix turnover associated with the termination of skeletal growth. Whether PLA2 is active in the cartilage matrix, its precise localization, and its effects on the resident chondrocytes remain to be determined. | |
| 2692819 | Diflunisal versus naproxen in the management of rheumatoid arthritis. | 1989 Nov | Diflunisal (500 mg orally, twice daily) and naproxen (375 mg orally, twice daily) were compared for efficacy and tolerability in a 12-week open-label study in 33 patients with active rheumatoid arthritis (RA). Both drugs resulted in marked reduction in the number of swollen, tender, and painful joints and comparable improvement in patients' assessment of disease activity and pain. There were no significant differences between the two medications in the measured indices of disease activity. No adverse experiences were reported by patients in either treatment group. The results indicate that both diflunisal and naproxen were equally effective and that both agents are generally well tolerated in this group of patients with RA. | |
| 2428100 | Circadian and diurnal variation of circulating immune complexes, complement-mediated solub | 1986 | Nine patients with active classical rheumatoid arthritis (ARA criteria) were studied with reference to circadian variation of immunological and clinical parameters. Complement-mediated solubilization (CMS) of immune complexes (IC) and the level of circulating IC were found to be inversely related with low CMS and increased IC levels in the morning, and vice versa in the afternoon. Bed rest and exercise did not influence these fluctuations. The C3d concentration in plasma was increased but showed no diurnal or circadian periodic fluctuations when the levels were corrected for fluctuations in plasma albumin concentration. Clinical assessment by means of pain score exhibited marked variations, with high scores in the morning, and lower in the daytime, whereas measurements of Ritchie's joint index showed no consistent pattern. The circadian variations in CMS, serum IC and clinical parameters indicate the need to collect blood specimens and perform clinical examinations of patients at a fixed time of day. | |
| 10290984 | Quality assurance review of a rheumatoid arthritis education program. | 1988 Dec | Quality assurance seeks to measure the degree of compliance to set standards. To determine compliance to a treatment plan of rest, prescribed joint exercise, and medication; 31 rheumatoid arthritis patients were telephoned 3 months after attending a rheumatoid arthritis education program. On follow-up, more than half of the patients reported being compliant. Those that were non-compliant listed reasons related to the affective learning domain rather than lack of cognitive learning. | |
| 2782974 | Correlation between synovial neopterin and inflammatory activity in rheumatoid arthritis. | 1989 Aug | According to recent investigations neopterin (a pyrazinopyrimidine derivative) is a biochemical marker that reflects the activity of the proinflammatory immunocellular system of the synovial tissue in rheumatoid arthritis (RA). Interferon gamma, derived from antigen activated T lymphocytes, stimulates macrophages to synthesise and release neopterin into the culture supernatant in vitro. To extend this in vitro model to a clinical level a sensitive new radioimmunoassay technique was used to measure neopterin concentrations in the synovial fluid (SF) of 17 patients with active RA, nine with osteoarthritis, and six with acute gout, and in that of 12 controls undergoing meniscectomy. The SF neopterin concentrations were significantly higher in patients with RA than in the other groups of patients, particularly the controls. Multivariant analysis showed that SF neopterin concentrations correspond better with the systemic inflammatory activity of RA than with the local disease activity of the knee joints. Thus the study strengthens the hypothesis that neopterin reflects the essential role of the activated immunocellular reaction in the pathogenesis of RA. | |
| 2970811 | The in vivo effect of triethylphosphine gold (auranofin), sodium aurothiomalate and azathi | 1988 Jul | The percentages of CD5+ (pan T), CD8+ (mainly T suppressor) and CD4+ (mainly T helper) cells were studied in 80 patients with rheumatoid arthritis (RA), grouped according to medication into four groups: 1) controls, not receiving remission-inducing therapy, 2) patients on oral gold (triethylphosphine gold, auranofin), 3) patients on parenteral gold (sodium aurothiomalate) and 4) patients on azathioprine. The total number of lymphocytes was not influenced by treatment with auranofin or sodium aurothiomalate, while the number of lymphocytes was depressed in the azathioprine-treated group (P less than 0.01). Neither treatment with azathioprine or parenteral gold, nor treatment with azathioprine influenced the CD4+/CD8+ ratio. | |
| 3442071 | [Thermologic studies in inflammatory rheumatic diseases--general presentation and personal | 1987 Nov 15 | The registration of the temperature of the body surface gains an increasing significance among others for the diagnosis of inflammatory rheumatic diseases. A criterion of the inflammation of the joint, the hyperthermy as well as the disturbances of the microcirculation may be made evident by means of the thermological methods. Own results in healthy persons and patients with rheumatoid arthritis as well as evidence of the literature confirm the possibilities of the measurements of temperature for the rheumatology. On account of the great inter- and intra-individual variability of the skin temperature an orientation at the temperatures of normal values of certain measuring regions is not reasonable. When the results of the temperature measurement are estimated, therefore, a comparison with temperature patterns of healthy persons must be done. Using dynamic measuring methods the evidence of thermological investigations may essentially be enlarged. The evaluation of the results of the measurement is increasingly performed with the help of computers. | |
| 2781473 | [Intra-articular administration of glucocorticoids in rheumatoid arthritis from the doctor | 1989 | Based on the opinions of 20 expert rheumatologists from two rheumatological hospitals a number of important assumptions are provided relative to the intraarticular administration of glucocorticoids in patients suffering from rheumatoid arthritis. The viewpoints appeared to be many and varied even within the frameworks of the same hospital. The ranging of some rehabilitation factors in certain parameters (efficacy, danger, availability, and so forth) demonstrated that the technique of intraarticular punctures is an effective and popular enough method for the treatment of patients suffering from rheumatoid arthritis. The doctors' assessments of the method were on the whole higher as compared to the patients' assessment (66 patients with rheumatoid arthritis). There were appreciable differences in the correlation ratios among the doctors as regards the assessments "effective--ineffective" and "I know it very well based on my own experience"--I know it poorly". These differences (from 0.10 to 0.89) evidence the existence of both authoritative doctors and doctors who rely on the generally accepted opinions rather than on their own experience, accounting for controversies in subjective estimates. | |
| 2681239 | Surgical treatment of the elbow in rheumatoid arthritis. | 1989 Nov | Major disability secondary to involvement of the elbow in RA is uncommon. The indications for surgery are pain and loss of motion unresponsive to medical management. With painful synovitis and decreased function, good results are obtained with synovectomy and radial head excision. Advanced joint destruction may require TEA which produces good to excellent results in 75 to 90 per cent of cases. Arthrodesis of the elbow is not a useful procedure in patients with rheumatoid arthritis and multiple joint involvement. Salvage procedures such as resection arthroplasty and distraction arthroplasty can produce fair results in most cases. | |
| 3266946 | The development of a C1q anti-C1q immunoadsorbent for removal of immune complexes from pla | 1988 Dec | An immunoadsorbent based on immobilized C1q has been developed to remove possibly pathogenic immune complexes from plasma deriving from patients suffering from systemic lupus erythematosus or rheumatoid arthritis. Traditional immobilization procedures based on, e.g., cyanogen bromide activation could not be used to produce an efficient adsorbent. However, by using antibodies directed towards C1q as handles for the immobilization of C1q it was possible to make an adsorbent that efficiently bound immune complexes in plasma. The capacity of the C1q anti-C1q adsorbent to bind artificial immune complexes such as aggregated human globulins or immune complexes from various plasma samples was evaluated. Both batch and column experiments were conducted. The typical capacity in batch was about 1 mg immune complexes/ml gel when incubated with patient plasma samples with high titers of immune complexes. Special attention has to be paid to leakage of undesirable components from the adsorbent. It was found that leakage of C1q occurred but it was not more than after covalent immobilization procedures such as cyanogen bromide. | |
| 3170771 | Representative sample of rheumatoid synovium: a morphometric study. | 1988 Aug | The synovium from 11 patients with rheumatoid arthritis, who were undergoing joint surgery, was assessed using histological and morphometric techniques. Histological examination confirmed previous reports that the intensity of the cellular reaction varied throughout the synovium, and the morphometric method reflected this variability sensitively. The method was shown to be reproducible and allowed areas of similar cellular density to be defined. From these defined areas a total of 2.5 mm2 of synovium equivalent to 12 fields at x250 required analysis to reflect the variation in the cellular reaction. It would be feasible to collect this amount of material using an arthroscope. | |
| 2285744 | Fatigue in rheumatoid arthritis. Conditions, strategies, and consequences. | 1990 Jun | Fatigue is a frequent and debilitating problem for people with rheumatoid arthritis (RA). In this descriptive study a Fatigue Interview Schedule was administered to 20 patients with RA to elicit symptom-specific information. Qualitative analyses resulted in the identification of descriptors of fatigue, conditions under which fatigue occurs, an intricate repertoire of strategies used to prevent and manage fatigue, and the consequences of chronic fatigue. | |
| 3427842 | Bilateral giant cyst of the shoulder. | 1987 Jul | The case of a 61 year old white female with a rapidly progressive rheumatoid arthritis who developed bilateral giant cyst of the shoulder is described here. Arthrographic investigation indicated that these giant cysts were true synovial cysts rather than "pseudocysts". | |
| 2661575 | Rheumatoid extensor tendon ruptures. | 1989 May | Rheumatoid tendon ruptures occur primarily by the effect of the tenosynovium on the tendon or secondarily by the effect of the tenosynovium on the bone. An understanding of these complications combined with a knowledge of the anatomy helps to explain the pathology and suggest possible treatments. | |
| 1703737 | Characterization of peripheral blood and salivary gland lymphocytes in secondary Sjögren' | 1990 Winter | Secondary Sjögren's syndrome (SS) is defined as a condition of patients with sicca symptoms in association with a connective tissue disease such as rheumatoid arthritis. This study was designed to investigate the peripheral blood and affected minor salivary gland (SG) tissue lymphocytes with monoclonal antibodies in patients with secondary SS having rheumatoid arthritis. Minor SG lymphocytes of the patients and normals were determined in the fresh-frozen sections of the minor SG biopsy samples using monoclonal antibodies with immunoperoxidase technique. Peripheral blood of secondary SS patients revealed significant reduction in CD3+ and CD8+ cells. CD4/CD8 radio, HLA-DR+ cells, and B-cells were unchanged. SG biopsies showed varying degrees of lymphocytic infiltration with predominance of CD3+CD4+ cells located at the periductal areas. CD8+ cells were found to be in low numbers within the infiltrates. IgG- and IgA-producing plasma cells were both numerous in the biopsy samples. Our findings suggest that there is an alteration of lymphocyte subpopulations at the local site of inflammation in the salivary glands without, however, a corresponding alteration in the peripheral blood. | |
| 3701743 | Mineral metabolism in postmenopausal women with active rheumatoid arthritis. | 1986 Feb | Serum and urinary variables of bone mineral metabolism were studied in 49 postmenopausal women with rheumatoid arthritis (RA). Results were compared to those in a sex, age and menopausal age matched control group. No patient took corticosteroids, or had any disease other than RA which might affect bone. Total serum calcium was low in patients with RA compared to controls (9.0 +/- 0.5 mg% vs 9.3 +/- 0.3 mg%, p less than 0.005), but was normal when corrected for albumin (9.5 +/- 0.6 mg% vs 9.3 +/- 0.4 mg%, NS). Serum phosphorus was significantly higher in patients with RA than in controls (3.6 +/- 0.3 mg% vs 3.3 +/- 0.4 mg%, p less than 0.001) as well as serum alkaline phosphatase activity (107.6 +/- 27.2 IU/l vs. 9.6 +/- 28.91 IU/l). Serum creatinine, vitamin D and parathyroid hormone levels were comparable in both groups. Urinary hydroxyproline and mucopolysaccharide excretions were higher in patients with RA than controls, both for fasting (respectively 0.089 +/- 0.028 vs 0.039 +/- 0.023, p less than 0.001 and 0.072 +/- 0.027 vs 0.047 +/- 0.019, p less than 0.001) and for 24 h values (50.3 +/- 17.9 mg vs 36.2 +/- 15.4 mg, p less than 0.001 and 54.6 +/- 26.0 mg vs 41.7 +/- 16.5 mg, p less than 0.05). Urinary calcium excretion was comparable in the 2 groups. Our findings of raised serum phosphorus and alkaline phosphatase activity, raised urinary hydroxyproline and mucopolysaccharides excretion suggest that in patients with RA there is a higher metabolic activity of bone. In none of the patients could any indication of osteomalacia or of parathyroid overactivity be found. | |
| 2319517 | Rifampin therapy in rheumatoid arthritis. | 1990 Feb | Several second-line antirheumatic agents possess both immunosuppressive and antimicrobial properties. Rifampin is an antimicrobial agent recently found to exhibit immunosuppressive activity in both animal and human studies. Intraarticular rifamycin SV, a rifampin derivative, has been reported to cause dramatic improvement in gonarthritis in 15 patients with rheumatoid arthritis (RA). These reports along with the personal observation of spontaneous improvement of arthritic symptoms in 2 patients with RA treated with rifampin at our institution, prompted us to conduct a pilot study using oral rifampin at 600-1200 mg daily in 8 patients with active, adult onset, seropositive RA. Although, no clinically important or statistically significant improvement occurred in any of the outcome variables measured (p greater than 0.12), the power of this study to detect such differences was limited. Alkaline phosphatase increased modestly in 7 patients. One patient developed an acute, drug induced, flu-like syndrome with marked elevation of liver enzymes which resolved promptly with drug withdrawal. We conclude that the potential effectiveness of oral rifampin therapy in RA is doubtful. |