Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 1912168 | [Lymphocyte subpopulations in rheumatoid arthritis patients, their relatives and spouses]. | 1991 Apr | A study of lymphocytic sub-populations of patients with rheumatoid arthritis, their families and partners, is presented. The determination was carried out by means of monoclonal antibodies, analysing: T cells sub-population (CD3, CD4, CD8 and quotient CD4/CD8); B cells sub-population (B1); activated cells (OK1a); monocytes and NK cells (OKM1 and BMA 070). The results showed an increase of CD3 sub-population (T total lymphocytes) in patients with RA and their families. The meaning of these results is difficult to evaluate as lymphocyte sub-population is affected by several variables. The lack of modification of suppressor population, CD4/CD8 quotient and IKM1 cells in RA patients is probably due to the moderate activity of the disease. | |
| 3394105 | [Quantitative and qualitative changes in the fibronectin level in rheumatoid arthritis]. | 1988 | Blood plasma and synovial fluid (SF) of 48 patients with rheumatoid arthritis (RA) were studied. Plasma fibronectin (FN) concentration measured by rocket immunoelectrophoresis was found to be almost the same in patients and in healthy donors (348.6 +/- 16.4 micrograms/ml and 354.3 +/- 10.2 micrograms/ml, respectively. The level of SL FN was shown to rise in RA patients up to 1069.7 +/- 157.2 micrograms/ml, whereas in healthy persons and in posttraumatic synovitis it was equal to 420.0 +/- 112.6 micrograms/ml (p less than 0.05). Using cross-immunoelectrophoresis, the heterogeneity of synovial FN was revealed which was determined by complex formation with hyaluronic acid. It was found that both plasma and synovial fluid possessed higher gelatin-binding activity when compared with control samples as studied by the ability to agglutinate gelatinized particles. Direct correlation was shown between disease development rate and bioassayable plasma and synovial fibronectin. | |
| 3345294 | Lack of evidence for the presence of neutrophil autoantibodies in the serum of patients wi | 1988 Jan | Sera of 22 patients with Felty's syndrome and 14 patients with rheumatoid arthritis were tested in assays routinely used for the detection of neutrophil antibodies (i.e. immunofluorescence, agglutination and cytotoxicity tests) as well as in the antibody-dependent lymphocyte-mediated granulocytotoxicity test. One or more of the routinely used assays were positive in a high percentage of the sera (77% and 64%, respectively). The antibody-dependent lymphocyte-mediated granulocytotoxicity test was only positive with sera of three patients with Felty's syndrome. Positive results in the immunofluorescence and agglutination tests could be attributed to the presence of immune complexes in the sera, whereas positive antibody-dependent lymphocyte-mediated granulocytotoxicity tests were probably due to the presence of HLA alloantibodies. It is concluded that serological tests routinely used for the detection of neutrophil autoantibodies should be interpreted with caution in patients with Felty's syndrome. Our results also indicate that the neutropenia in Felty's syndrome is rarely, if ever, due to neutrophil-specific autoantibodies. | |
| 3532352 | Psychological aspects of pain in rheumatoid arthritis: a review. | 1986 | Pain in rheumatoid arthritis (RA) is reviewed from a socio-cognitive perspective. Questions are raised about the usefulness of the distinction between organic vs non-organic pain patients, and alternative explanations for the behaviour of seronegatives is presented. A case based on physiology and behaviour is made for more studies of the acute and prechronic stages of RA. Four major areas of methodology are considered: studies of lay explanations about RA show that people hold relatively accurate views about the nature of pain in RA, and where causal explanations are given for pain and illness, this is conducive to good mental health. A section on the measurement of pain addresses issues about the applicability and standardisation of scales, and welcomes the move from the exclusive use of quantitative to qualitative multidimensional measures. The pain language of RA is described. Experimental studies of pain in RA appear to be of limited use. A review of activities and functional disability indicates that cognitive and behaviour modification techniques appear to have most promise in motivating chronic patients to be more active, and to comply with medication, as well as improving mental health. In a discussion of successful therapies, the principles of reducing uncertainty and increasing perceptions of control are the underlying features. However the reliability of cognitive therapies in the treatment of RA so far remains unproven. | |
| 10149645 | Total ankle arthroplasty: new concepts and approaches. | 1991 Apr | A new type of total ankle arthroplasty developed with the use of a CAD-CAM computer was used in a series of 27 ankles between 1985 and 1989. Follow-up in 19 ankles ranging from 18 months to 5.5 years postoperatively indicates that the design of this implant appears to avoid the problems of subsidence and malleolar impingement. Based upon the results in this series, it appears that the best candidates for use of this type of total ankle implant are patients with rheumatoid arthritis. | |
| 3625467 | Appraisals of control and predictability in adapting to a chronic disease. | 1987 Aug | In a sample of 92 patients with rheumatoid arthritis, we examined interrelations among various control appraisals, illness predictability, psychosocial adjustment, mood, and illness status. Perceiving greater personal control over the disease and symptoms and perceiving greater health-care-provider control over symptoms were associated with greater illness predictability. Patients reported more personal control over their symptoms than over the course of the disease and thought that their health care providers had more control over disease course than they did themselves. Multiple regression analyses showed that perceiving greater personal control over one's medical care and treatment was associated with positive mood and psychosocial adjustment. Negative mood was also associated with the belief that providers have greater control over the patient's daily symptoms. Patients who had a more severe disease and expressed greater personal control over its course reported greater mood disturbance and were rated as exhibiting less positive adjustment, but those who had more severe daily symptoms and expressed greater personal control over their symptoms reported less mood disturbance. These findings are discussed in terms of the possible benefits of patients' active participation in their care and the implications of perceiving personal and others' control over more or less controllable aspects of the illness, especially when the illness is more severe. | |
| 2475988 | [Group-specific antigens of streptococci in the make-up of circulating immune complexes in | 1989 | The level of "A" and "B" streptococcal antigens in the circulating immune complexes, structure and the level of antibodies to the group specific streptococcal polysaccharides in the patients with minimal activity and latent course rheumatism and in the patients with rheumatoid arthritis of different duration is studied. It is shown that the concentration of the "A" group streptococcal antigens in the circulating immune complexes is higher in rheumatic patients, than in healthy subjects and tonsillitis patients. The high "B" group streptococcal antigen content is found in the patients with rheumatoid arthritis. A high titer of antibodies to streptococcal polysaccharides of "B" group, especially in the synovial fluid was revealed. A possibility of streptococcal antigens and their antibodies' participation in the development of pathologic process is discussed. A value of obtained indexes in the diagnosis of small activity rheumatism and early stages of rheumatoid arthritis is shown. | |
| 3673159 | [Skin changes in an acute attack of polyarthritis--Aschoff's nodules and rheumatic granulo | 1987 Aug 15 | Exanthematous skin eruptions in an acute attack of polyarthritis histologically showed Aschoff's nodules or rheumatic granulomas, which are usually found in the myocardium but not in the skin. | |
| 3827331 | Demonstration of an unidentified 48 kD polypeptide in circulating immune complexes in rheu | 1987 Feb | Circulating immune complexes (CIC) were isolated from 25 patients with rheumatoid arthritis (RA) by anti-C1q affinity chromatography. The components were detected by silver stained sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels and identified by the Western blot. The CIC were composed of 20 different polypeptides, including albumin, immunoglobulin, complement, and acute phase reactants. Two components (molecular weight 48 kD and 45 kD respectively) remained unidentified. The 48 kD polypeptide was found in CIC from six out of 14 patients (43%) with extra-articular RA, but from none of eight patients with vasculitic complications of other connective tissue diseases. All immunoreactants were more frequently found in the patients with extra-articular features of RA. Although these results emphasise that most CIC in RA are composed of endogenous proteins, the 48 kD polypeptide is a candidate for an extrinsic antigen in RA. | |
| 2880632 | Sulphasalazine alone or in combination with D-penicillamine in rheumatoid arthritis. | 1987 Feb | Thirty patients (22 women) with active rheumatoid arthritis participated in an open study of 6 months' treatment with either enteric-coated sulphasalazine (SASP) or SASP plus D-penicillamine (DPA). Patients were assessed at regular intervals using a number of clinical and biochemical tests designed to detect specific antirheumatic activity. There were significant improvements in clinical and laboratory variables with both regimens consistent with second-line activity. Improvements were greater and more numerous with combination therapy. At the end of the trial period, there were nine 'responders' in the SASP/DPA group but only six in the SASP group. Neither efficacy nor toxicity could be related to patient acetylator status. Nausea and dyspepsia were frequent problems with both treatment regimens but dysgeusia and thrombocytopenia were confined to the SASP/DPA group. Study withdrawals were twice as common with combination therapy. These results suggest that a combination of SASP and DPA is more potent than SASP alone but at the expense of poorer patient tolerance. | |
| 2727042 | Effect of antirheumatic drugs on lymphocyte membrane fluidity in rheumatoid arthritis: a f | 1989 | We have demonstrated significant differences in 1,6-diphenyl-1,3,5-hexatriene fluorescence polarization values between lymphocyte membranes of untreated rheumatoid arthritis patients and lymphocyte membranes of patients treated with antirheumatic drugs, such as hydroxychloroquine and auranofin. No difference has been detected in rheumatoid arthritis patients treated with auranofin associated with 6-methylprednisolone. The results indicate that some drugs used in the treatment of rheumatoid arthritis induce changes of lymphocyte membrane fluidity. The positive correlations between fluorescence polarization values and objective indices of the disease activity, as erythrocyte sedimentation rate, C-reactive protein, fibrinogen level, and alpha 2-globulin, suggested that fluorescence polarization could be used in the study of the pharmacological action of antirheumatic drugs and in monitoring antirheumatic therapy. | |
| 3028287 | Deficient control of in vitro Epstein-Barr virus infection in patients with ankylosing spo | 1986 Dec | Infectious Epstein-Barr (EB) virus obtained from the B95-8 marmoset cell line was used to infect mononuclear cells from healthy controls and patients with rheumatoid arthritis (RA) and ankylosing spondylitis (AS), and outgrowth of B cells into lymphoblastoid cell lines was assessed by visual microscopy and uptake of tritiated thymidine over a 28 day period. When undiluted virus was used lymphocytes from both patients with AS and RA and from normal controls outgrew into lymphoblastoid cell lines (LCLs) by day 28 of culture. At dilutions of 1/10, 1/20, and 1/40, however, the control cells showed regression of proliferation at approximately day 14 of culture, whereas the cells from patients with AS and RA continued to proliferate and outgrew into LCLs (transformation scores of cells from patients with AS compared with controls at day 28 p less than 0.05 in all cases; thymidine uptake at a 1/40 dilution at day 28, patients with AS compared with controls p less than 0.01). Hence these results suggest that there is a defect in the cellular response to EB virus induced B cell proliferation in patients with AS similar to that seen with cells from RA donors. | |
| 3264771 | Heterogeneity of T cell receptor idiotypes in rheumatoid arthritis. | 1988 Sep | The nature of the T cell response in the rheumatoid synovium was investigated by using monoclonals MX9 and 42/1C1, which recognize the V beta 8 and V beta 5 T cell receptor gene families respectively. The blood and synovial T cells of ten patients with rheumatoid arthritis were compared. The majority (8/10) had different numbers of V beta 5 and V beta 8 cells in the joints from those in the blood, indicating that the T cells in the joints were not a sample of those in the blood. In three patients both V beta 5 and V beta 8 cells in the joint were augmented in number, suggesting that the T cells selectively retained in the joint were not members of a single clone, but derived from many clones. Some patients had increased levels of V beta 5 or V beta 8 alone in the joint indicating that heterogeneity existed between patients. These results are not consistent with the preferential or dominant use of a single V beta gene family in the T cells involved in the rheumatoid arthritic joints. | |
| 2071618 | Survivorship analysis of the uncemented porous-coated anatomic knee replacement. | 1991 Jul | Ninety-six patients who had a total of 108 replacements with an uncemented porous-coated anatomic knee prosthesis were followed for an average of sixty-four months (range, thirty-nine to ninety-three months). At the time of the most recent follow-up, twenty-one implants (19 per cent) had failed, all due to problems with the tibial component. A patellar component was not used, and no noteworthy patellar problems were encountered after the operation. There were no infections about the prostheses, and no femoral implant was revised. The most common cause of failure was collapse of the anteromedial part of the tibial plateau, which occurred in fourteen knees. The prosthesis loosened without collapse of bone in two knees, and five knees were revised because of gross wear of the polyethylene. When the time of failure was defined as the point at which revision of the prosthesis was recommended, the cumulative rate of survival was 84 per cent (95 per cent confidence interval, +/- 7 per cent) at five years and 77 +/- 10 per cent at six years. When the time of failure was defined as the point at which the knee replacement was actually done, the five-year rate of survival was the same. No significant differences were demonstrated between groups that were stratified by age, sex, weight, or primary diagnosis. We therefore do not recommend the use of an uncemented porous-coated anatomic knee replacement of the design that was evaluated in this study. | |
| 1848332 | Characterization of platelet-derived growth factor beta-receptor expressing cells in the v | 1991 Mar | Platelet-derived growth factor (PDGF) beta-receptor expression in normal and rheumatoid synovia was investigated by double immunofluorescence staining of frozen sections and by in situ hybridization. In the inflamed synovia, PDGF beta-receptor mRNA was present in vascular cells, as well as in discrete stromal cells. PDGF beta-receptor expressing cells in rheumatoid synovia were characterized by double immunofluorescence staining using the PDGFR-B2 monoclonal antibody at a concentration at which this antibody merely stained granular accumulations of PDGF beta-receptors. Granular accumulations of PDGF beta-receptors were articulate in blood vessel cells, but also appeared in discrete stromal cells. Thus, the overall distribution of cells having granular accumulations of PDGF beta-receptors was similar to the distribution of cells expressing PDGF beta-receptor mRNA. Double immunofluorescence stainings showed that: (a) a majority (greater than 90%) of resident macrophages did not express granular PDGF beta-receptor staining, but macrophages were often juxtaposed to PDGF beta-receptor-positive cells; (b) T lymphocytes did not express PDGF beta-receptors, but these cells were frequently found in the proximity of cells stained by PDGFR-B2; (c) in some blood vessels both HLA-DR expressing cells and PDGF beta-receptor expressing cells could be visualized, whereas in other blood vessels, cells expressing only one of these activation markers could be detected; (d) smooth muscle cells in blood vessels contained PDGF beta-receptors; and (e) capillary endothelial cells in the inflamed synovia recurrently displayed granular PDGF beta-receptor staining. The granular accumulations of PDGF beta-receptors may reflect internalization of the receptor as a result of paracrine or autocrine ligand stimulation. In support of such a possibility are the findings that elevated levels of PDGF B chain mRNA were detected by in situ hybridization in the inflamed synovia, and that cells expressing PDGF B chain mRNA were distributed similarly to cells expressing PDGF beta-receptor mRNA. Taken together, the results indicate that PDGF has a role in the inflammatory process in rheumatoid synovitis, most likely by stimulating proliferative events in the vasculature. | |
| 2904267 | Structure of T cell antigen receptor beta chain in synovial fluid cells from patients with | 1988 Dec | We attempted to identify a clonal proliferation of T cells from synovial fluid samples from patients with rheumatoid arthritis, using techniques of restriction fragment length polymorphism. We used probes for the beta chain of the T cell receptor to analyze restriction fragments prepared from the genomic DNA of synovial fluid mononuclear cells from 10 patients and synovial fluid T cell preparations from 5 additional patients. The results demonstrated unarranged (germline) T cell receptor gene fragments of DNA in all cell preparations, indicating the lack of clonality of rheumatoid arthritis synovial fluid T cells. | |
| 2029206 | Circadian rhythm in pain, stiffness, and manual dexterity in rheumatoid arthritis: relatio | 1991 Apr | Fourteen patients with rheumatoid arthritis (RA) self rated their pain and stiffness on separate 10 cm visual analogue scales and performed bead intubation coordinometry (BIC) on six occasions each day for seven consecutive days. In addition, 14 healthy controls matched for age and sex also performed BIC measurements according to the same schedule. Data were analysed using least squares and cosine vector techniques. Significant circadian rhythms in patients with RA were detected in pain, stiffness, and BIC, and in controls in BIC. Pain was least in patients with RA at 1700 and stiffness at 1724. Peak BIC performance occurred almost simultaneously in RA (1544) and control (1528) subjects and for subjects with RA occurred within the 95% confidence interval of least pain and stiffness. These data suggest that the inferior performance of subjects with RA may be an accentuation of the normal physiological variation seen in healthy controls, but may be modulated by the patient's level of pain or stiffness, or both. | |
| 1927356 | Occipito-cervical fusion in rheumatoid arthritis. | 1991 | Between 1977 and 1988 twenty-five patients with rheumatoid arthritis involving the cervical spine were treated by an occipito-cervical fusion. Twenty-two patients are included in this study. The main indications for surgery were intractable pain and progressive neural impairment. The operative procedure consisted of an H-bone graft fixed with steel wire in 19 cases, fusion of occiput-C2 six times and of occiput-C3 thirteen times. A Hartshill-Ransford (5, 12) loop was used in three cases: occiput-C3 twice and occiput-C7 once. The mean duration of follow-up was 3 years 8 months in 17 patients. Improvement of symptoms and signs was achieved in 88%. There were 2 post-operative deaths. The high rate of pseudarthrosis in our series was confirmed by several authors. The loop technique provides a more stable fixation and will be our technique of choice in the future. | |
| 3245825 | Pancytopenia induced by low-dose methotrexate for rheumatoid arthritis. | 1988 Aug | Four cases of pancytopenia related to low-dose weekly pulse methotrexate therapy for rheumatoid arthritis are described. All patients were aged above 60 years and had renal impairment. In every case marrow recovery followed withdrawal of methotrexate. However, one patient developed pneumonitis and died. Cholangitis, respiratory infection and increase in methotrexate dose were precipitating factors. Pharmacokinetic data indicated prolonged tissue drug exposure in two cases studied; dose-related toxicity was further supported by successful resumption of methotrexate in reduced dosage in two cases. It is possible that methotrexate dose should be modified during intercurrent illness in patients older than 60 years who have renal impairment. | |
| 3532402 | [Changes in the levels of soluble HLA antigens and their light chains (beta 2 microglobuli | 1986 | An increase in the level of soluble HLA-A and B antigens in the blood serum of patients with rheumatoid arthritis and systemic lupus erythematosus was observed using IKO-1 monoclonal antibodies and polyspecific alloantisera. This increase was accompanied by the elevation of the concentration of beta 2-microglobulin (the light chain of the molecules of HLA-antigens of the 1st class). A degree of the concentration of soluble HLA-antigens and beta 2-microglobulin depended on a degree of disease activity, a variant of a clinical course and the presence of visceral involvement. |