Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 3017876 | In vitro production of collagen by synovial fibroblasts from D-penicillamine-treated arthr | 1986 | In order to get further insight into the mechanism of D-penicillamine action on synovial tissue collagen synthesis, fibroblasts derived from drug-treated arthritic rabbits were cultured and labelled with radioactive proline. No evident correlation was found between the amount of newly synthesized collagen and the previous treatment of animals. In contrast, the prolyl-hydroxylase activity was reduced in cells from rabbits receiving D-penicillamine. This finding suggests that culture conditions may influence the collagen-synthesizing potentiality of the synovial fibroblasts without changing the level of enzyme activity. Therefore, the prolyl-hydroxylase activity could be considered here as a more reliable reflection of the in vivo situation. The ratio of type III to type I procollagens, as estimated by DEAE-cellulose chromatography, showed a rise in cultures from D-penicillamine-treated rabbits as compared to controls. This result indicates that long-term administration of the drug may alter the collagen composition of synovial tissue matrix in rheumatoid arthritis. The question remains, however, whether this alteration contributes to the beneficial effect of the drug. | |
| 2024642 | GM-CSF in the treatment of Felty syndrome. | 1991 May | Many therapeutic agents have been tried with variable success in the treatment of Felty neutropenia, but the reports are anecdotal. We now describe the second trial of recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF), in a splenectomized, infected patient with Felty syndrome. | |
| 2330924 | Use of the Saxon test as a measure of saliva production in a reference population of schoo | 1990 May | The Saxon test, a simple, reproducible, and low-cost technique to test for xerostomia in adults was applied to 347 schoolchildren who were free from any known disease. The values in children differed significantly from the values in adults and increased slightly with age. The test can easily be performed on samples kept for 8 days at 4 degrees C or at room temperature. | |
| 3473623 | Smell and nasal findings in patients with primary Sjögren's syndrome. | 1986 | As part of a clinical trial of the effect of Efamol on primary Sjögren's Syndrome (SS), 36 patients were interviewed about nasal symptoms and examined for sense of smell and nasal mucociliary clearance. The sense of smell was examined by quantitative olfactometry using coffee as a stimulant while mucociliary clearance was evaluated by the saccharin test. The findings were compared with those of an age and sex matched control group. 39% of the patients complained of dryness of the nose and 44% of nasal crust formation whereas none of the healthy controls had such complaints. In contrast no differences in sense of smell (subjectively as well as objectively) and mucociliary clearance could be demonstrated. Neither was there any correlation between mucociliary clearance and crust formation or dryness of the nose. Also the mucociliary clearance was not correlated to the "break up time" of the tear-film determined by the ophthalmologist. The present findings indicate that examination of the sense of smell and nasal mucociliary clearance is of little diagnostic value in primary SS and cannot be used for monitoration of disease activity. | |
| 2269793 | Traumatic and spontaneous dislocation of extensor tendon of the long finger. | 1990 Nov | Extensor tendon dislocation is an unusual problem in patients unaffected by rheumatoid arthritis. Sixteen cases of ulnar dislocation of the long extensor tendon of the long finger are reported. Five of these cases were classified as traumatic dislocation and 11 as spontaneous dislocation. All cases were treated by surgery. Differences in anatomic and clinical features between traumatic and spontaneous dislocation of the long extensor tendon are described. | |
| 3946008 | Selective absence of immunoglobulin A1 or A2 among blood donors and hospital patients. | 1986 Jan | A hemagglutination inhibition assay was developed for the determination of immunoglobulin A1 and A2 (IgA1 and IgA2) with subclass-specific rabbit antiserums. Among 93,020 apparently healthy blood donors, selective absence of IgA1 was found in six and that of IgA2 in 15, at a prevalence much higher than the absence of total IgA that was revealed by only one donor. Among 6800 hospital patients with various disorders, the absence of IgA1 was found in one with breast cancer and that of IgA2 in two with rheumatoid arthritis. | |
| 1716941 | Whipple's disease, familial Mediterranean fever, and adult-onset Still's disease. | 1991 Aug | Whipple's disease is a multisystem disorder thought to be caused by infection by rod-shaped bacilli. Early diagnosis remains difficult, because initial clinical features are nonspecific. Ultrasonography and computed tomographic scanning were used to demonstrate distinctive lymphadenopathy in Whipple's disease. Magnetic resonance imaging showed central nervous system lesions that were reversible with antibiotic therapy. Familial Mediterranean fever, or recurrent polyserositis, is an autosomal recessive disorder common among patients of Mediterranean heritage. Erysipelas-like skin lesions are commonly described. Other skin lesions, including Schönlein-Henoch purpura, nonspecific purpura, diffuse erythema, and angioneurotic edema are now reported. Renal complications, thought previously to be due primarily to amyloid, are also caused by immunoglobulin deposition resulting in mesangial proliferative glomerulonephritis. Adult-onset Still's disease is a systemic illness characterized by quotidian fever and a fleeting, salmon-colored rash. The long-term evolutions of juvenile-onset and adult-onset Still's disease were compared and found to be similar, except for the occurrence of amyloidosis in the latter group of patients. Prognosis of patients with articular features was worse than that of patients with extra-articular features. A multicenter survey of Japanese patients found few significant differences between Japanese and non-Japanese cases. Less well-recognized features of adult-onset Still's disease, including neurologic complications, uveitis, and peritonitis, are reported. | |
| 1989079 | Primary Sjögren's syndrome associated with inappropriate antidiuretic hormone secretion. | 1991 Jan 19 | A patient in whom primary Sjögren's syndrome and inappropriate antidiuretic hormone secretion were associated is reported. This is the first report of such an association. The possible pathophysiological mechanisms are discussed and vasculitis proposed as the underlying pathogenetic mechanism. | |
| 3050706 | Viruses and salivary gland disease: are there associations? | 1988 Aug | Viruses can cause sialadenitis and may be associated with other diseases of salivary glands, particularly immunologically mediated and neoplastic lesions. The evidence that such an association with Sjögren's syndrome is causal is reviewed here and shown to be fairly tenuous at present. | |
| 3473653 | Ultrastructural study of the lacrimal gland in a case of Sjögren's syndrome. | 1986 | A 78-year-old woman, affected by a Gougerot-Sjögren's syndrome, had a typical keratoconjunctivitis sicca associated with xerostomia. A biopsy of lacrimal and accessory salivary glands was studied by conventional histological methods. In addition, the lacrimal gland was studied by electron microscopy. The two varieties of gland showed lesions of the same intensity with a similar decrease of lactoferrin and lysozyme. However, the ultrastructural features noted in the lacrimal gland were suggestive of an immunological reaction different from that noted in salivary glands. The attrition of ductal epithelia by lymphocytes was obvious but was never followed by regeneration and ductal ectasia. The massive infiltration of lymphocytes in the lobules and the early sclerosis of gland tissue appeared to be the main factor. Apparently, the sclerosis was not consecutive to the activity of periductal myoepithelial cells but resulted from the activity of fibroblasts in the production of basal membranes and collagen connective tissue. | |
| 2628148 | [The protein-blood volume indices during moderate plasmapheresis]. | 1989 Dec | Quantitative characteristics of changes in the volume of circulating plasma and the amount of circulating protein were studied in rheumatoid arthritis patients before and after plasmapheresis of moderate intensity. Basing on the comparison of the precalculated and estimated parameters the authors have suggested 4 mathematical models equally describing the protein-volemic relationships in the body. Satisfactory correlation has been recorded between the estimated and precalculated values of protein concentration in the plasma after plasmapheresis that enables data collection in a short cut program. | |
| 3742305 | [Mortality after regular implantations of total hip prostheses]. | 1986 May | Operative and post operative (two months) mortality among 1280 patients undergoing total hip replacement is studied. Mortality rate was 1%. The main cause is pulmonary embolism, then mental deterioration and anaesthesia. Use of methylmethacrylate did not cause death. Rheumatoid arthritis could increase the risk. | |
| 3554884 | Failures with the Judet noncemented total hip. | 1987 Feb | Primary failures with 36 noncemented Judet total hip arthroplasties are reported. Steep-cup inclination in 7 cases resulted in two dislocations, two migrations, one skew insertion, and two painful hips. Moreover, two migrations were encountered in patients with rheumatoid arthritis. Femoral shaft fractures occurred intraoperatively in 7 cases and postoperatively in another 2 cases. In addition, major fractures of the greater trochanter occurred in 3 cases. The reoperation rate was 11/36, due to primary failures 8-37 months postoperatively. Consequently, the Judet design cannot be recommended for noncemented use. | |
| 2961576 | CD3+4-8-WT31-(T cell receptor gamma+) cells and other unusual phenotypes are frequently de | 1987 Dec | T lymphocytes (E rosetting cells) isolated from the joint fluid of four patients with juvenile rheumatoid arthritis (JRA) were first analyzed for surface antigen expression. Approximately 15% of cells were CD25+ (interleukin, IL, 2 receptor positive), in addition, a remarkable proportion of cells expressed the CD2+3- phenotype. CD3+ cells outnumbered the sum of CD4+ and CD8+ cells as well as the cells reactive with the WT31 monoclonal antibody (which recognizes a framework determinant of the alpha/beta T cell receptor). Purified T cells were cloned under culture conditions (1% phytohemagglutinin, PHA plus IL2) which allow clonal expansion of most peripheral blood T lymphocytes. Under these conditions proliferating cells ranged from 25 to 65%; clones (derived from microcultures containing 0.5 or 0.25 cells/well) were tested for cytolytic activity against P815 cells (in the presence of PHA) or against the natural killer (NK)-sensitive K562 target cells. Fifty-four percent and 73% of clones obtained from the two patients with the polyarticular form of the disease displayed cytolytic activity in the lectin-dependent assay. Cytolytic clones were 22 and 29% in the two patients with single joint involvement. About half of all cytolytic clones displayed NK-like activity. Surface antigen analysis revealed that, in addition to conventional CD3+4+8- and CD3+4-8+, a noticeable fraction of clones (50/202) displayed unusual surface phenotypes. In particular, 33/50 coexpressed CD4 and CD8 antigens; 7/50 were CD2+3-4-8- and displayed NK-like activity; 10/50 expressed CD3 but lacked both CD4 and CD8 antigen and did not react with the WT31 monoclonal antibody. In order to allow selective growth of IL2-responsive cells, T lymphocytes were also cloned directly in IL2. As much as 57% of all clones thus obtained (48/84) displayed cytolytic activity. Moreover, about half expressed unusual surface phenotypes including CD2+3-4-8-, CD3+4+8+ and CD3+4-8-WT31-. Given the accumulation at the site of the joint involvement of unusual T cells, most of which displayed cytolytic activity and were likely to represent cells activated in vivo (IL2 responsive), one may speculate that these cells may be involved in the injury process. | |
| 3117066 | Effects of gold sodium thiomalate on interferon stimulation of C2 synthesis and HLA-DR exp | 1987 Sep | Gamma-interferon (gamma-IFN) is a T cell-derived lymphokine that has potent macrophage-activating properties. It increases Fc receptor density, increases the formation and release of reactive oxygen intermediates, increases the synthesis and release of complement cascade proteins, especially C2 and factor B, and increases class II (HLA-DR) antigen expression. These effects may play a role in the potentiation of inflammation in rheumatoid arthritis. We examined the possibility that gold sodium thiomalate (GST), an effective treatment for rheumatoid arthritis, would inhibit gamma-IFN-mediated stimulation of monocyte/macrophages. GST in concentrations attainable in vivo was shown to inhibit both spontaneous and gamma-IFN-stimulated C2 production up to 50%. GST inhibition could be only partially overcome with increasing concentrations of gamma-IFN. In addition, GST inhibited gamma-IFN-stimulated HLA-DR expression at the highest concentrations tested (20-50 micrograms/ml). GST alone in low concentrations (0.1-5 micrograms/ml) was found to increase HLA-DR antigen expression as quantitated by several methods, including flow cytometry, cell surface enzyme-linked immunosorbent assay, and Western blotting. This GST-stimulated increase in HLA-DR antigen expression paralleled an increased ability of monocytes to present antigen. The mechanism by which low concentrations of GST stimulate HLA-DR antigen expression is unclear, but was shown by 35S-methionine cell labeling not to involve increased HLA-DR protein synthesis. | |
| 2355296 | High susceptibility of human articular cartilage glycosaminoglycan synthesis to changes in | 1990 Jul | The effect of sulfate concentration in the medium on glycosaminoglycan synthesis in articular cartilage of five different species was examined in relation to the physiological serum sulfate concentration in these species. Only the rate of sulfated glycosaminoglycan synthesis in human articular cartilage was sensitive to small deviations from the physiological sulfate concentration. A reduction in the sulfate concentration from 0.3 mM (physiological) to 0.2 mM resulted in a 33% reduction in glycosaminoglycan synthesis. In addition, we studied the effect of arthritic and "osteoarthritic" alterations in murine cartilage on the dependence of glycosaminoglycan synthesis on low sulfate concentrations. Arthritic and "osteoarthritic" cartilage had a similar dependence on the sulfate concentration in the medium as normal cartilage. Glycosaminoglycan synthesis in human articular cartilage appears to be very sensitive to the potential sulfate-depleting effects of drugs used in the treatment of rheumatoid arthritis and osteoarthritis. | |
| 2460538 | Substance P, neurokinin A, and neurokinin B induce generation of IL-1-like activity in P38 | 1988 Nov 15 | Near nanomolar concentrations of substance P induce production of IL-1 or an IL-1-like activity in the mouse macrophage cell line P388D1. Moreover, this could be accomplished with the carboxyl-terminal octapeptide substance P4-11, and could be inhibited with the substance P antagonist [D-Pro2, D-Trp7,9]-substance P. Two other mammalian neurokinins, neurokinin A and neurokinin B, were also found to induce secretion of IL-1-like activity in P388D1 cells. These findings suggest that activation of immune cells by neuromodulators can contribute to the maintenance of the chronic inflammatory state and the immunopathology observed in arthritic disease mediated by IL-1. The results also suggest that one approach to the treatment of rheumatoid arthritis might be to attempt to inhibit the local effects of immuno-modulatory neuropeptides, specifically the neurokinins, in affected joints. | |
| 2626126 | A case study of illicit preparation of antirheumatic analgesic with phenylbutazone as acti | 1989 Jun | The abuse of phenylbutazone among rheumatoid arthritis patients has recently become a subject of interest. Unscrupulous manufacturers take advantage of the miraculous analgesic property of phenylbutazone and deliberately add this toxic drug in their preparations without declaring its presence on the label. In a recent survey, many such illicit preparations were seized from Chinese medical halls in Johor and sent to the Department of Chemistry, Johor Bahru for analysis. Here a Gas Chromatograph Mass Selective Detector (GC-MSD) method was developed for the determination of phenylbutazone in illicit traditional preparations. | |
| 2474585 | Severe secondary amyloidosis in a dog with dermatomyositis. | 1989 May | A male collie aged 5 years 10 months, which developed dermatomyositis at 2 months of age, died from severe secondary amyloidosis. Amyloid deposition was most severe in renal glomeruli and produced renal failure. Amyloidosis has been reported in man with immune-mediated disorders including rheumatoid arthritis, systemic lupus erythematosus and dermatomyositis. It is possible that the inflammation in this case of familial canine dermatomyositis may have predisposed to the development of amyloidosis. | |
| 2476081 | Lack of antikeratin antibodies in patients with palmoplantar pustular eruptions and arthro | 1989 | Assessment of antikeratin antibodies (AKAs), using an indirect immunofluorescence technique with rat esophageal keratin as antigen, was performed in 14 patients with arthropathy and palmoplantar pustular eruptions, six of whom also had psoriasis vulgaris. Twelve patients had seronegative spondyloarthropathy. They were all AKA negative. Two patients had classical seropositive-erosive rheumatoid arthritis (RA), and were both AKA positive. This suggests that AKA is not related to the arthropathy associated with pustulosis palmoplantaris or psoriasis, or to the presence of pustular eruptions on the palms and soles. The finding of AKA in RA is in keeping with previous findings. |