Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 6197377 | Beta-thromboglobulin in inflammatory synovial fluid. | 1983 Dec | Activated platelets release substances which potentially can contribute to joint lesions in inflammatory arthritides. To elucidate a possible participation of platelets in inflammatory joint reactions, the concentrations of the platelet protein beta-thromboglobulin (beta-TG) were measured in 90 inflammatory synovial fluids. Seven percent of the patients with rheumatoid arthritis and none of the patients with other inflammatory joint diseases (e.g., Reiter's disease, reactive or crystal arthritides) had beta-TG concentrations in synovial fluid exceeding the upper normal range of plasma beta-TG. The absent or very modest signs of local platelet activation were contrasted by the pronounced neutrophilic and monocytic activation, as assessed by the measurements of some granule proteins: lactoferrin, myeloperoxidase, lysozyme, and ferritin. No correlation was found between these inflammatory cell markers and beta-TG. A positive correlation (p less than 0.001) was noted between beta-TG and beta 2-microglobulin, which appeared in particularly high amounts in rheumatoid arthritis. This correlation may reflect a disturbed permeability of synovial membrane for LMW proteins or a related activation of platelets and lymphocytes. The present results do not give any evidence of platelet activation playing a major role in proliferative or destructive processes in arthritis. | |
| 7270335 | Immune enhancement of granuloma formation as an experimental model for the pannus of rheum | 1980 Dec | The pannus of rheumatoid arthritis is a proliferating mass of various cells and tissues, including follicles of lymphoid cels, and is the major agent of destruction of the surrounding structural tissues. Infiltrating lymphocytes are thought to be the prime stimuli for the growth of the pannus, through the secretion of of lymphokines. In contrast to this high turnover, destructive rheumatoid granuloma, most commonly used experimental granuloma models are of the non-immune, low turnover variety and thus give a rather poor reflection of clinical conditions. A model has been developed in our laboratory in which lymph node cells are removed from Freund's complete adjuvant sensitized rats and injected into sponge implants in normal syngeneic recipient animals, leading to enhancement of granuloma formation. This response has been characterized and shown to be related to lymphocyte activation and to the sensitivity of rats to polyarthritis induction. The lymphocyte transfer model may be useful in studying the action of drugs on lymphocyte mediated chronic inflammatory responses and the principle of lymphocyte transfer may be applied to other hypersensitivity granuloma models. | |
| 911353 | The activating effect of synovial fluid and washings of synovial membrane on autologous ly | 1977 Sep | The effect of synovial fluid and washings of synovial membrane on autologous lymphocytes from patients with rheumatoid arthritis and other diseases has been studied using a rapid method based upon the increase in intranuclear birefringence occurring in the early stages of lymphocyte activation. Retardation of polarized light indicating increased lymphocyte activation was seen in lymphocytes from patients with rheumatoid arthritis but not in lymphocytes from patients with other diseases. | |
| 7259331 | Polymorphonuclear granulocytes in rheumatic tissue destruction. III. an electron microscop | 1981 Aug | Metatarsophalangeal and metacarpophalangeal joints from 3 patients with rheumatoid arthritis were investigated electron microscopically with regard to the occurrence of polymorphonuclear granulocytes (PMNs) at the pannus-cartilage junction. In all 3 cases PMNs could be detected at the junction and within the cartilaginous matrix. PMN cytoplasmic processes surrounded collagenous islands in the cartilage. From the morphological findings it is deduced that PMNs are cells capable of destroying cartilage in inflammatory joint diseases, in particular in rheumatoid arthritis. | |
| 3885876 | Effect of drug therapy on circulating and synovial fluid Ig-secreting cells in rheumatoid | 1985 Apr | A common immunological abnormality in rheumatoid arthritis (RA) is an increased spontaneous polyclonal B cell activation. In order to study the influence of drug therapy in RA on the functional activity of B cells we enumerated spontaneous plaque-forming cells (PFC) in peripheral blood lymphocytes (PBL) and synovial fluid lymphocytes (SFL) by a reverse haemolytic plaque assay. Spontaneous IgG-, IgM-, and IgA-PFC in PBL of 26 patients with classical erosive RA receiving either gold salts or D-penicillamine were similar to those observed in 20 healthy controls. In contrast, significantly higher numbers of IgG- and IgA-PFC, but not IgM-PFC, were found in PBL of nine patients with classical erosive RA receiving non-steroidal anti-inflammatory drugs (NSAID) alone. Furthermore, spontaneous PFC in SFL from 16 consecutive patients with RA receiving second-line drugs, as well as 17 patients with other forms of arthritis (non-RA) were generally low and significantly less than those observed in 20 RA patients on NSAID alone. Moreover, a wide individual variation in PFC, especially in relation to the IgG class, was recorded in the synovial lymphocytes. These studies imply that treatment with second-line drugs is associated with normalisation of B cell activity in RA patients, and that the effect can be detected at the cellular level both in blood and synovial fluid. | |
| 401699 | Fenoprofen, aspirin, and gold induction in rheumatoid arthritis. | 1977 Jan | Fenoprofen calcium (2,400 mg/day) or aspirin (3,900 mg/day) was administered in double-blind fashion to 20 rheumatoid patients during 6 months of gold induction therapy, and to 20 rheumatoid patients not receiving gold. Among both the gold-treated and nongold-treated patients, the fenoprofen and aspirin groups improved equally in all but one parameter of disease activity. Fenoprofen and aspirin did not differ significantly in the observed prevalences of abdominal discomfort, guaiac-positive stools, or peptic ulcers. Aspirin was associated with significantly higher mean serum glutamic oxaloacetic transaminase (SGOT) levels than fenoprofen, but only among patients undergoing gold induction. Comparison of efficacy parameters between patients treated with gold and patients treated with oral drugs alone revealed significant differences favoring gold. | |
| 4094952 | Multiple replacements of major joints in rheumatoid arthritis. | 1985 Jan | Multiple total joint replacements were performed in 51 handicapped rheumatoid patients. Three joints were replaced in 23 patients, four joints in 25 patients, five joints in one patient, and six joints in two patients, for a total of 186 replacements of elbow, hip, knee and ankle joints. By the time of followup, seven of the patients had died due to unrelated causes. Evaluation of the walking ability in the remaining 43 patients revealed that 20 out of 22 nonambulatory patients had become able to walk indoors and outdoors, and 21 patients who had been able to walk only indoors became able to walk outdoors as well. Major complications occurred in 11 patients: femoral neck fracture in three, supracondylar fracture of the femur in three, cervical myelopathy in two, and loosening of the femoral prosthesis in two patients. The factors responsible for poor results in two patients were cervical problems and loss of motivation. | |
| 1057866 | Synovial membrane and fluid morphologic alterations in early rheumatoid arthritis: microva | 1975 Jun 13 | Eight patients have been studied during the first 6 weeks of rheumatoid synovitis. All of them exhibited microvascular injury, which was manifested by gaps between endothelial cells, vascular occlusion, erythrocyte extravasation, or endothelial cell injury. In four patients, a variety of virus-like particles were found associated with the endothelium or perivascular cells. In two cases, particles were seen in electron-dense deposits in vessel walls. Lymphocytes and PMN infiltrated the synovial membranes, but plasma cells were uncommon. Evidence of phagocytosis was prominent in synovial lining cells and other large mononuclear cells, but not in PMN. These observations are consistent with injury to synovium and, specifically, synovial vessels as an early stage in RA synovitis. The virus-like particles require further investigation, because nonviral cell components remain very difficult to distinguish in electron microscopy tissue sections. | |
| 495563 | Performance of latex-fixation kits used for serologic diagnosis of rheumatoid factor in rh | 1979 Oct | Ten latex-fixation kits (both slide and tube) were evaluated by use of a reference serum containing 250 IU of rheumatoid factor (Communicable Disease Center). All tests were performed with serial dilution of serum; adjacent tubes differed by a factor of 0.10 log10. Geometric titers, means, standard deviations, and variance analysis were used to evaluate commercial kits. Three levels of variance were applied: (1) 0.18 or less for intralaboratory variation when the same serum is tested with the same kit; (2) 0.18-0.35 when various lots or batches of the same serum were used; (3) 1.0 or less when various commercial kits were tested with the same serum. The sensitivity of each commercial kit was determined by assigning to it a value (the minimum detectable unit) arrived at by dividing the number of units in the standard by the titer obtained. The technic presented could be applicable to other serologic tests, and thus provide a general method for their standardization and quality control. | |
| 6698957 | Anti-protein kinase NII antibodies in rheumatic autoimmune diseases. | 1984 Feb 25 | Antibodies against nuclear protein kinase NII were detected by radioimmunoassay in the sera of patients with systemic lupus erythematosus and mixed connective tissue disease but not in sera from normal individuals or from patients with rheumatoid arthritis. Immunoglobulins derived from sera containing the anti-kinase antibodies inhibited the activity of protein kinase NII in vitro but had no effect on the activity of cytoplasmic cyclic AMP-dependent protein kinase. | |
| 7352412 | A clinical evaluation of a card agglutination test for rheumatoid factor. | 1980 Jan | The Macro-Vue Card Test for Rheumatoid Factor (Hynson, Westcott, and Dunning) is a new commercially available card agglutination test designed for qualitative and quantitative procedures. Coded sera from 195 patients, six known positive sera, and two positive serum pools were tested using four commercial kits. The commercial kits evaluated included latex (Hyland RA Test, Behring-Rapi/tex RF Test), sheep erythrocytes (Wampole, Rheumaton), and charcoal (HWD-Macro-Vue RF Card Test) as carrier particles. Using the prescribed quantitative procedures, the percentages of positive and negative results for rheumatoid factor detected by each product were determined after the (serum) code was broken. The Macro-Vue test compares well with the Behring and Hyland latex dilution systems, and appears to detect more positive sera than the Wampole test. Titers obtained using the Macro-Vue test were more reproducible day to day with known positive sera than were titers obtained using any of the other kits. | |
| 946664 | Rheumatoid arthritis with rheumatoid heart disease and granulomatous aortitis. | 1976 Jun 7 | Aortic insufficiency and aortitis are frequent complications of ankylosing spondylitis but are considered rare in rheumatoid arthritis. A 49-year-old woman with severe rheumatoid arthritis had the cardiovascular changes common to both diseases. At autopsy, the heart and aorta showed granulomatous ane fibrinous pericarditis and epicarditis, "core" granulomas in the aortic valve cusps and mitral valve leaflets, and coronary arteritis. These are considered to be classical changes of rheumatoid carditis. In addition, there were mesoaortitis with obliterative endarteritis of the vasa vasorum and fibrosis of the aortic cusps with separation of the commissures. These are considered to be changes of ankylosing spondylitis. This case, therefore, represents a mixed form of cardiovascular involvement within the spectrum of the rheumatoid diseases. | |
| 6203543 | Fibronectin on the surface of articular cartilage in rheumatoid arthritis. | 1984 Jun | The presence of fibronectin on the surface of articular cartilage in rheumatoid arthritis (RA) was investigated. Cartilage samples were stained by the immunoperoxidase method using anti-human fibronectin antibody, and observed under light and electron microscopy. Fibronectin was present on the articular surface in 7 of 8 RA patients. The degree of staining varied greatly among the patients. Five of 8 patients were positive for fibronectin in 50% or more of the cartilage areas studied. In total, fibronectin was observed in RA. Fibronectin was not observed in cartilage samples of osteoarthritic joints or joints which were not diseased but had undergone trauma. Ultrastructurally, it was observed to be associated with collagen fibrils and amorphous substance in the matrix. The fibronectin-negative surface of the rheumatoid cartilage was usually thick ultrastructurally, compared with the fibronectin-positive surface, and the staining for fibronectin roughly correlated with decreased proteoglycans on the surface. The presence of fibronectin in the matrix appeared to be revealed by partial degradation of proteoglycans with proteolytic enzymes in the synovial fluid, as well as by the deposition of fibronectin onto the surface of rheumatoid cartilage. Fibronectin on the articular surface may play an important role in promoting pannus extension onto the articular surface in RA. | |
| 1167808 | Elevated plasma sialyltransferase in the cancer patient. | 1975 Mar | Sialyltransferase activity was measured in plasma samples using desialated fetuin as the acceptor and cytidine 5'-phosphate-sialic acid as donor. The data show an increased enzyme level in 56 of 65 cancer patients studied, as compared with normal control values. The enzyme was not significantly elevated during lacation or in liver cirrhosis, but it was elevated in rheumtoid arthritis. Of the patients with cancer, 35 showed plasma enzyme levels above any value encountered in rheumatoid arthritis plasmas. The determinants of enzyme level in cancer appear to be complex: monitoring of plasma sialytransferase may be of value in measuring tumor progression, metatastatic involvement, or success of therapeutic programs. | |
| 6826595 | Infection after total elbow arthroplasty. | 1983 Mar | Deep sepsis occurred after fourteen (9 per cent) of 156 elbow-replacement procedures in 140 patients. This high frequency of infection was attributed to several factors. First, the patients were drawn from a population that was at high risk of infection, because rheumatoid arthritis and post-traumatic arthritis were the indications for arthroplasty. Second, many of the patients had had prior surgery, which significantly (p less than 0.02) increased the risk of sepsis in those with rheumatoid arthritis. Third, some patients had surgery after the arthroplasty, which also seemed to predispose to deep infection (p less than 0.05). In one patient the elbow was salvaged by early débridement, and in two others reimplantation of a total joint replacement was successful after removal of the first prosthesis and control of the infection. Resection arthroplasty was required to arrest the infection in ten patients, eight of whom had a satisfactory result. The high incidence of this significant complication attests to the hazardous nature of the elbow-replacement procedure and should warn orthopaedic surgeons to be cautious when recommending this form of treatment. | |
| 1202606 | Co-cultivation of human cell lines with synovial fluids of patients with rheumatoid arthri | 1975 | We have interpreted the above findings as implying that the changes seen may have arisen as a result of co-cultivation, albeit accidental, of Chang cells and cells derived from RA synovial tissue or fluid. A further obvious approach to this problem is to attempt deliberately to repeat the co-cultivation of Chang cells with RA synovial fluids. | |
| 307389 | HLA-D and Ia antigens in rheumatoid arthritis and systemic lupus erythematosus. | 1978 Jun | The HLA-D region in the HLA chromosomal complex is concerned with cellular interactions and regulation of the immune response. Rheumatoid arthritis (RA) has been found to be associated with one of the HLA-D antigens. In two separate studies from this laboratory it was found that HLA-Dw4 was more frequent in RA patients than in normal controls. The patients were Caucasians suffering from erosive, rheumatoid factor positive RA. Combined results of the two studies showed HLA-Dw4 to be present in 52% of the 130 patients, compared to 13% in 119 controls (P less than 10(-6). Ia antigens were determined serologically in isolated B cells. The antigen Ia4, associated with HLA-Dw4, was increased in RA patients (70%), compared to normal controls (28%). In SLE, typing was difficult because of fragility of the B cells. Preliminary results in 14 SLE patients suggested that Ia4 was also increased in this disease. It seems likely that these associations are a reflection of genetic factors related to the immune response involved in the pathogenesis of RA and perhaps also of SLE. | |
| 722027 | Interpositional arthroplasty for rheumatoid carpometacarpal joint disease. | 1978 Nov | Over the past 3 years the Swanson great toe prosthesis was used in an interpositional arthroplasty at the carpometacarpal joint in 21 thumbs of 19 patients with rheumatoid arthritis and three thumbs of two patients with lumpus arthritis. In selected patients with advanced disease, this prosthesis has the advantage of allowing for correction of deformity while preserving carpal stock which may be important if a surgical procedure is required at the wrist. Stable capsular closure does not require reinforcement by adjacent tendon. Twenty-two thumbs of 19 patients were improved, although in three the prosthesis was malpositioned. Two patients required reoperation and removal of the prosthesis: one for infection, and one for failure to correct deformity. Overall functional results often were affected by the status of disease in other joints, but in general were acceptable. | |
| 6531714 | Psychological approaches to the management of arthritis pain. | 1984 | The present review examines the literature regarding the efficacy of cognitive-behavioral and other self-control interventions in helping arthritis patients reduce their pain and functional disabilities. The evidence indicates that self-control interventions have produced significant and positive changes in the pain and functional disabilities of patients with rheumatoid arthritis and arthritis secondary to hemophilia. However, the literature suffers from deficiencies with regard to the use of small subject samples; inadequate control procedures and follow-up assessments; failure to demonstrate that positive outcomes are related to changes in subjects' covert experiences or control of physiological variables; and reliance upon self-report measures of outcome. The review is followed by a description of a multidisciplinary study of the efficacy of a biofeedback-assisted, cognitive-behavioral group therapy program for rheumatoid arthritis patients that features several methodological improvements relative to previous investigations. The preliminary outcome data show that the cognitive-behavioral intervention is associated with reductions in pain behavior and self-reports of pain and disability. It is concluded that, although the self-control interventions have shown promising results, psychologists must demonstrate positive and reliable outcomes among large numbers of arthritis patients over extended periods of time if the interventions are to be viewed as credible by rheumatologists. | |
| 290815 | Trace element determination in serum by proton-induced x-ray emission. | 1979 | We report the use of proton-induced x-ray emission (PIXE) as an analytical method in clinical research. The experimental set-up and target preparation procedures are briefly discussed together with the methods of automatic data acquisition and analysis. Results from a clinical project involving rheumatoid patients receiving chrysotherapy are presented. |