Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 6776937 | Influence of gold salts on the content of trace elements in serum of patients with rheumat | 1980 | In 25 patients with rheumatoid arthritis under Sanocrysin treatment the influence of gold salts on the content of trace elements was tested. The results show that in future the elements iron and copper during the Sanocrysin therapy should be taken into consideration. | |
| 1257685 | [Contribution of scanning electron microscopy to the study of normal and pathological huma | 1976 Jan | Scanning electron microscopy permits three-dimensional morphological study of the outer layer of the synovial membrane. This article concerns the study of 15 surgical samples of normal and pathological human synovial membranes: 3 normal synovial membranes, 6 inflammatory synovial membranes, 5 arthrosic synovial membranes, 1 tuberculous tenosynovitis. The samples were washed, fixed in a mixture of glutaraldehyde and iso-osmotic paraformaldehyde, dehydrated in increasing concentrations of alcohol and at the critical point, and then subjected to double metallization (aluminium and gold). Certain portions were treated with hyaluronidase. Hypertrophy of the villi, considerable in inflammatory synovial membranes, minimal in arthrosic synovial membranes, and absent in the normal synovial membrane, was confirmed. The outer layer is composed of cells of different sizes with spaces betwen, in which images indicating intercellular bridges can just be seen. The surface of the cells is irregular and covered with numerous microvilli. The arthrosic synovial membrane is poorly with large cells; in contrast in an inflammatory synovial membrane they are numerous, globular, and project from the surface. | |
| 778989 | A trial of micro-encapsulated and enteric-coated aspirin in rheumatoid arthritis. | 1976 May | In a trial of 48 patients with rheumatoid arthritis, enteric-coated aspirin (4.55 g daily( and micro-encapsulated aspirin (4.50 g daily) proved to be equally effective in reducing morning stiffness, relieving pain, increasing grip strength, reducing ESR, and reducing the need for additional analgesic tablets, compared with placebo. Reduction of joint tenderness was also found, but this was not statistically significant. Proximal interphalangeal joint circumference altered little during the trial. Tinnitus and deafness were commoner with enteric-coated aspirin, but gastric side-effects were similar. Of 39 patients completing the trial, there was an equal patient preference for enteric-coated aspirin and micro-encapsulated aspirin. Salicylate side-effects necessitated withdrawal of six patients from the trial and dose reduction in nine patients. It was concluded that the efficacy and side-effects in rheumatoid arthritis of both aspirin preparations were similar. | |
| 679615 | Synovial rupture of the knee joint: confusion with deep vein thrombosis. | 1978 Jul | Four patients were admitted to hospital with a provisional diagnosis of deep vein thrombosis. Arthrography of the knee joint subsequently demonstrated the presence of synovial cysts in all four patients with rupture into the tissues of the calf in two cases. These conditions are difficult to distinguish clincally and radiological investigation may be required to provide the correct diagnosis. Synovial rupture should be suspected in patients with knee effusions who develop acute pain and swelling of the calf, particularly if the patient has rheumatoid arthritis. | |
| 676428 | Simple method of circulating immune complex detection in human sera by polyethylene glycol | 1978 Jun | A rapid test for detection of circulating immune complexes in a small serum sample was developed to facilitate clinical diagnosis of immune complex disorders. The test is based on a selective precipitation of soluble circulating complexes of antigen-antibody in 3.75% concentration of high-molecular polyethylene glycol. Precipitation is followed photometrically at 450 nm, 1 cm cuv. after 1 h incubation at room temperature. Comparison of E450 values in groups of patients with immune complex disorders, such as rheumatoid arthritis, systemic lupus erythematosus and glomerulonephritis, with healthy controls or patients with non-immunological disorders revealed highly significant differences. Sera of all patients with high clinical activity of disease exhibited positive reaction. In 121 human sera the results of this examination were compared with the results of C 1 q binding test. There was 73.5% agreement between the results of both methods. Our test is more rapid, suited for routine clinical use. | |
| 6592880 | [Molecular biology studies of synovial fluid cells of patients with chronic polyarthritis | 1984 | In the course of molecular biological research on patients with classical rheumatoid arthritis (according to ARA criteria), an investigation was carried out on the influence of different doses of prednisolone on semiconservative DNA synthesis, the sedimentation of nucleoids before and after gamma-irradiation and protein synthesis in synovial fluid cells. A significant dose-dependent suppression of semiconservative DNA synthesis was demonstrated with 5, 10 and 25 mg prednisolone. Granulocytes and cell detritus were removed by Ficoll urografin separation. In the presence of high saline concentrations and non ionic detergents nucleoids were extracted from synovial cells. These contained, besides nuclear proteins, almost the complete nuclear RNA and DNA in a superhelical structure. After ultracentrifugation, the nucleoid sedimentation profiles were determined by saccharose gradients. The individual sedimentation profiles were determined and statistically evaluated before and after irradiation causing strand breaks by Co60 at a dose of 1 Gy. No difference in nucleoid sedimentation was seen at a dosage of 5 mg of prednisolone, but after administration of 10, 25 and 40 mg a decrease in sedimentation velocity was observed and this effect might be explained by the DNA destabilizing effect of a steroid receptor complex followed by DNA strand breaks which had migrated into the nucleus. In vitro, no significant inhibition of supercoiled DNA repair was found after gamma-irradiation when prednisolone was administered. This is an argument against a damaging influence of prednisolone on the DNA repair of the genetic material of synovial fluid cells, which are predominantly lymphocytes. For the investigation of protein synthesis, 3H-labelled L-aminoacids were incorporated into the cells, and the activity measured after repeated washings. Following the administration of the lower prednisolone doses (5 and 10 mg) the incorporation of 3H-labelled L-aminoacids increases, whereas a significant inhibition of protein synthesis is found with doses of 25 mg or more. The possible clinical relevance of this phenomenon is discussed. | |
| 901594 | Secretion of plasminogen activator by rheumatoid and nonrheumatoid synovial cells in cultu | 1977 Jul | The secretion of plasminogen activator by human synovial cultures established from explants was studied. Thirteen cultures established from patients with rheumatoid disease were hyposecretors of plasminogen activator. Seven cultures derived from patients with nonrheumatoid joint disorders all secreted high levels of this enzyme. Rheumatoid and nonrheumatoid cells contained comparable levels of intracellular activator. It is suggested that the secretion of plasminogen activator is a function of normal synovium and that a defect in this function in the rheumatoid synovium perpetuates the inflammatory process. | |
| 6586155 | Temporal arteritis presenting as a symmetrical peripheral polyarthritis. | 1983 Dec | An 83 year old female presenting with a symmetrical peripheral polyarthritis resembling rheumatoid arthritis, subsequently developed biopsy-proven temporal arteritis. This case report draws attention to this uncommon presentation of temporal arteritis and briefly reviews common presenting features of this condition. | |
| 876914 | Sarcoma complicating therapy with cyclophosphamide. | 1977 Jan | A patient is described who developed a poorly differentiated sarcoma after cyclophosphamide was used to treat his rheumatoid arthritis. This case emphasizes the importance ot considering neoplastic disease as a potential hazard associated with the use of immunosuppressive drugs. | |
| 6903082 | [A benign form of rheumatoid arthritis, associated with low IgG, IgM and IgA and C3-proact | 1980 May | In 31 case studies, 29 patients diagnosed with definite rheumatoid arthritis and 2 patients with probable rheumatoid arthritis were investigated. The duration of the disease averaged 8 years with 3 years being the minimum. These patients received either no basic therapy or had not been treated six months previous to the investigation. Patients with low levels of immunoglobulins were shown to have significantly better functional capacity (Steinbrocker) and significantly less synovitis, erosions and joint destructions despite longer duration of the disease than the control group with higher levels of immunoglobulins and C3-proactivator. All patients with this benign development of rheumatoid arthritis had an IgG-level below 1000 mg/100 ml (mean 767 mg/100 ml +/- 166) and low levels of IgA (mean 194 mg/100 ml +/- 73), IgM (mean 111 mg/100 ml +/- 53) and C3-proactivator (mean 23 mg/100 ml +/-6). 50% of these patients had positive rheumatoid factors. | |
| 7010518 | [Enzymo-immunologic determination of the class of antiglobulin factors]. | 1980 Oct | The authors describe an enzymo-immunologic method of determination of the class of rheumatoid factors (RF). This technic includes 3 main stages : 1) extraction of RF by fixation on aggregated rabbit IgG previously adsorbed on the walls of a plastic tube; 2) recognition of RF linked to the solid phase by anti-IgG addition, and addition of human IgA and IgM linked to glucose oxidase; 3) revelation of the enzyme activity linked to the solid phase : this enzyme activity directly depends on the levels of RF to be estimated. The results are expressed as a percentage of the fixation of a reference serum obtained from a patient with rheumatoid arthritis (RA). This serum presents an antiglobulin activity in the 3 major classes of immunoglobulins. The coefficients of variation of intra- and intersystem reproductibility lay between 5,6 and 17.8%. The specificity of the estimation was controlled by the localisation of the antiglobulin activity of the sera after chromatography on Sephadex G200 and by estimation of RF after their absorption by an aggregated IgG immunoadsorbant. This technic was applied to the identification of RF in RA (90 cases); in diseases not related to RA and in control subjects : the sensitivity of this method appears greater than that of technics previously described (immuno-fluorescence, Rose-Waaler reaction) in fact, only 12,2% of sera of patients with RA remain negative with this new test. However, this greater sensitivity does not seem to have altered the clinical specificity of the test : in fact, only 8,4% of the sera of patients with diseases unrelated to RA are positive by this method, which, moreover, may be explained by the age of these subjects (average age 71.5 years). | |
| 6274359 | Release of collagenase, neutral protease, and prostaglandins from cultured mammalian synov | 1981 Nov | Hydroxyapatite (HA) and calcium pyrophosphate dihydrate (CPPD) crystals were phagocytosed when added to cultured human rheumatoid or normal canine synovial cells. Collagenase and neutral protease secretion into the culture medium was increased 5- to 8-fold over control values in the presence of HA and increased 3-fold in the presence of CPPD crystals. HA but not CPPD crystals induced a 300-fold increase in human rheumatoid synovial cell culture fluid prostaglandin (PG) E2 levels and an 8-fold increase in PGF alpha levels. This mechanism may be important in the pathogenesis of the destructive arthropathies associated with HA and CPPD crystals. | |
| 2997867 | [Differential arthrographic diagnosis of the painful shoulder joint]. | 1985 Oct | 60 arthrograms of the shoulder were explored under morphologic and pathologic-anatomic aspects. Differentiation was made between acute and chronic pain. The possible findings in leaking of the contrast material out of the joint capsule are shown and discussed. Signs are quoted of degeneration of soft tissue and bone. The findings in patients with rheumatoid arthritis and a history of shoulder dislocation are summarised. Distension of the joint capsule without shoulder dislocation is described. Filling of the periarticular lymphatics is mentioned in different cases. | |
| 517549 | Effect of hemodialysis on plasma naproxen concentration. | 1979 Nov | A case describing the effect of hemodialysis on naproxen plasma levels in a 48-year-old anuric man with chronic glomerulonephritis is reported. The patient received 500 mg of naproxen daily for rheumatoid arthritis while undergoing hemodialysis thrice weekly. The patient's mean pre-dialysis plasma concentrations were similar to those seen in normal volunteers, suggesting no accumulation of the drug. Plasma concentrations were higher after dialysis, probably because of fluid loss during dialysis. Naproxen is not cleared from the body during hemodialysis and, therefore, a post-dialysis naproxen dose is not necessary. | |
| 266170 | Acute leukaemia during azathioprine therapy. | 1977 Mar | Various tumours, in particular lymphomas, have been reported in association with azathioprine treatment, both after renal transplantation and in other conditions. A case is reported here of acute myeloid leukaemia associated with azathioprine therapy for rheumatoid arthritis, and this is now the third case reported in the world literature of leukaemia arising during azathioprine therapy for a non-malignant condition. | |
| 645427 | Immersion technique in soft tissue radiography of the hands. | 1978 | Soft tissue radiography of hands using the technique of mammary radiography and immersion in a 2.5 cm layer of 1:1 water-ethanol solution is evaluated. Using immersion the average background density decreases with a factor of about 2.5:1, with little deterioration in resolution (MTF). The immersion procedure makes the demonstration and evaluation of soft tissue swelling and periarticular oedema easier. | |
| 7056032 | Synovial fluid and plasma fibronectin levels in rheumatoid arthritis. | 1982 Jan | 1. Plasma fibronectin levels were similar in 60 healthy subjects and 88 with the rheumatoid arthritis. 2. In 42 patients with rheumatoid arthritis synovial fluid fibronectin levels were significantly higher than plasma levels (P less than 0.001). Intermediate fibronectin levels were found in synovial fluid from six patients with psoriatic arthritis, eight patients with osteoarthritis and seven with seronegative arthritis. 3. Plasma and synovial fluid fibronectin levels were not related to indices of inflammatory activity such as the erythrocyte sedimentation rate, the Ritchie articular index or synovial fluid cell counts. Nor did fibronectin behave as an acute-phase protein. 4. Immunofluorescent studies showed that fibronectin was adsorbed on fibrinous debris in rheumatoid arthritic joints. 5. These findings suggest that there is local production of fibronectin by the synovium and suggest that measurement of fibronectin levels in the synovial fluid may serve as an indicator of the tissue response to rheumatoid arthritis. | |
| 7436574 | Circulating and intra-articular immune complexes in rheumatoid arthritis: a comparative st | 1980 Oct | The C1q binding assay and the nephelometric monoclonal rheumatoid factor assay were able to discriminate 79% and 57% respectively of rheumatoid arthritis (RA) patients from healthy blood donors. In addition these assays could distinguish those patients with active arthritis from those with inactive disease, and the C1q binding assay correlated significantly with other laboratory indices of the rheumatoid process, including the erythrocyte sedimentation rate, low molecular weight or 7S IgM, and the rheumatoid factor titre. High levels of C1q binding were also seen in rheumatoid vasculitis. Both assays gave higher mean values in synovial fluid compared with the corresponding serum, but it appeared from ultracentrifugal analysis and from a lack of a consistent correlation between these assays that each assay was measuring different forms of immunecomplex-like material which may be involved in the immunopathogenesis of this disease. The C1q binding assay is of some value in the laboratory assessment of rheumatoid arthritis and appears to offer greater advantages than the monoclonal rheumatoid factor assay, although the usefulness of this latter assay may be very dependent on the monoclonal rheumatoid factor used. | |
| 3000493 | Results of total knee replacement using an uncemented tibial component. | 1985 Fall | Fourteen patients (16 knees) underwent total knee arthroplasty with a noncemented tibial component. Clinical evaluation at one year and two years showed a level of function and pain relief comparable to that reported in studies of cemented prostheses. Radiographic studies demonstrated evidence of active bone implant consolidation. | |
| 2413204 | Hypothesis: the nervous system may contribute to the pathophysiology of rheumatoid arthrit | 1985 Jun | No current theory of the mechanisms involved in the pathophysiology of rheumatoid arthritis (RA) explains its important clinical features. We hypothesize that neural mechanisms are involved in this pathophysiology and they explain at least 3 clinical features: specific high risk joints are more likely to develop arthritis; specific high risk joints have more severe arthritis; and RA is bilaterally symmetric. If our hypothesis is correct, it will provide a rationale for the development of new therapies for what is now an inadequately treated disease. |