Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 6342625 | Low-dose D-penicillamine therapy in rheumatoid arthritis. A controlled, double-blind clini | 1983 May | Two hundred twenty-five patients with active severe rheumatoid arthritis were admitted to a multiclinic, controlled, double-blind trial comparing the use of 500 mg D-penicillamine per day, 125 mg D-penicillamine per day, and placebo. One hundred seventy-one patients completed at least 30 weeks of therapy. The 500 mg D-penicillamine group demonstrated statistically significant improvement over the placebo group in grip strength, average circumference of swollen proximal interphalangeal joints, and patient assessment. While the trend was for greater improvement with the larger dose of D-penicillamine, there was no statistically significant difference among the 3 groups in duration of morning stiffness, walking time, physician's assessment, number of swollen joints, or scores for tender and swollen joints. The slight increase in efficacy of higher dose D-penicillamine was associated with increased toxicity. | |
| 6981388 | IgE and IgE-rheumatoid factors in circulating immune complexes in rheumatoid arthritis. | 1982 Aug | The sera of 21 patients with rheumatoid arthritis (RA), 11 patients with systemic lupus erythematosus (SLE), and 20 healthy subjects were analysed for the presence of IgE in immune complex fractions. These fractions were isolated by polyethylene glycol precipitation and gel filtration. Thirteen sera from RA patients contained IgE immune complexes (IC) and 11 of these were from patients with extra-articular manifestations. One SLE and none of the control sera contained such material. The serum IgE level did not correlate with IgE content of the IC fractions. Higher mean serum IgE levels were found in RA patients with extra-articular complications than in controls or RA patients with joint disease only, but the differences did not reach statistical significance. IgE anti-rabbit IgG (IgE rheumatoid factors) could be demonstrated in some IgE positive IC fractions. Antibodies to IgE, in 2 instances characterised as belonging to IgG class, were also found in ICs. This suggests the presence of anti IgE complexes. It is suggested that IgE, including some with rheumatoid factor activity, is contained in complexes which may be involved in some extra-articular manifestations of RA. | |
| 3873318 | Problems in ANA test interpretation: a comparison of two substrates. | 1985 | Comparison of serum antinuclear antibody (ANA) test results on commercial HEp-2 cell culture preparations and fixed mouse kidney sections demonstrated significant differences in end-point titers and pattern production. When manufacturer's suggested screening titers are used, there is also a significant difference in qualitative results and correlation with clinical status. With individual intralaboratory establishment of screening titer levels, some of these differences become less significant, although this study suggests that mouse kidney substrate slides are more sensitive in detecting nonspecific ANA, and that HEp-2 substrate slides are more specific in detecting ANA from cases of systemic lupus erythematosus. Antinuclear antibody substrate selection must be based on classic sensitivity-specificity considerations and the clinical correlation performance desired. Comparisons of interlaboratory or follow-up ANA results are invalid without consideration of substrate variations. Regardless of substrate utilized, each laboratory should establish its own individual screening titers in relation to suitable age groupings. | |
| 4036091 | [Cytochemical study of lymphocyte nucleoproteins in rheumatoid arthritis patients]. | 1985 | The purpose of the study is to investigate and juxtapose the cytochemical characteristics of ribonucleoproteins (RNP) and deoxyribonucleoproteins (DNP) in the lymphocytes of patients with rheumatoid arthritis (RA), of healthy lymphocytes and lymphocytes blast-transformed by phytohemagglutinins. Significant differences in the staining of lymphocytes for nucleoproteins were established in all 54 patients with RA studied. The unidirectional changes in the morphological form of the staining for RNP and DNP in blast-transformed lymphocytes and lymphocytes in RA is, most likely, in connection with the transition of the latter into active functional state and their participation in the forming of the immune inflammation. No significant difference was established in the dependence on the RA form--sero-positive or sero-negative. Regardless of the negative serological samples in the second form, there were some changes in the lymphocytes--carriers of immune reactions in organism. | |
| 389957 | Anti-cartilage antibody. | 1979 Aug | Antibody to cartilage has been demonstrated by indirect immunofluorescence on rat trachea in the serum of about 3% of 1126 patients with rheumatoid arthritis. Titres ranged from 1:20 to 1:640. The antibody was not found in 284 patients with primary or secondary osteoarthritis or in 1825 blood donors, nor, with the exception of two weak reactors, in 1314 paraplegic patients. In most cases the antibody appears to be specific for native type II collagen. Using this as an antigen in a haemagglutination test 94% of anti-cartilage sera were positive, whereas among 100 rheumatoid control sera there were only three weak positives. More than 80% of patients with antibody had some erosion of articular cartilage, but there was no correlation with age, sex, duration of disease, nor any recognisable clinical event or change. | |
| 264945 | High condylectomy for treatment of arthritis of the temporomandibular joint. | 1977 Apr | A review of 55 high condylectomies is presented with a discussion of diagnosis, rationale, technique, some simultaneous problems, and complications. An average two-year follow-up shows good results, and the procedure is thought to be useful when indicated. Many of our respondents, who classified themselves as improved, were actually cured of their arthritis, but had a previously diagnosed synergistic disorder. Muscle spasms and anxiety syndromes frequently required continued treatment after a 6- to 12-month interval. Despite the difficulty in diagnosis and the frequent occurrence of simultaneous disorders there is a definite place for the high condylectomy. Its use, however, is still restricted at this institution to that group of patients with persistent pain in the TMJ or severe functional disorders caused by actual pathologic conditions of the condyle. After surgery, all patients should be followed for at least two years for recurrence of any TMJ-related disorder. | |
| 443883 | Synthesis of sulphated proteoglycans by rheumatoid and normal synovial tissue in culture. | 1979 Apr | Synthesis of sulphated proteoglycans by cell lines derived from explants of 7 rheumatoid and 9 normal specimens of synovial tissue, as well as by 7 lines of skin fibroblasts from non-rheumatoid patients, was examined. Cells of all 3 types were cultured as monolayers. They were then disaggregated and their capacity to synthesise proteoglycan estimated in cell suspensions by the incorporation of [35S]-sulphate into CPC-precipitable material during 2 hours of incubation. Cell suspensions incorporated somewhat more [35S]-sulphate than corresponding duplicate monolayers. Synovial cells from rheumatoid patients incorporated 2 to 3 times as much [35S]-sulphate as synovial cells from normals. Skin fibroblasts, however, incorporated less [35S]-sulphate than rheumatoid or normal synovial cells up to the fifth passage. Thereafter their incorporation gradually increased to overtake that of synovial cells. About one-half to one-third of the total [35S]-sulphate labelled material was closely associated with cells from synovial tissues and fibroblasts respectively. | |
| 814535 | [Acute leukemia with megakaryoblasts. Incidence following immunosuppressive treatment of r | 1975 Nov 22 | The authors report the case of a man suffering from rheumatoid arthritis treated for almost 5 years with chlorambucil. Death occurred 9 years after the onset of the disease at the age of 55 years with a picture of acute megacaryoblastic leukaemia. | |
| 6292536 | Acute polyarthritis associated with active Epstein-Barr virus infection. | 1982 Dec 10 | Nine patients with an initial onset of symptoms of acute arthritis within the preceding four weeks were enrolled in a prospective serological study with clinical follow-up for six months to two years. Four adults with chronic rheumatoid arthritis and ten healthy adults were similarly studied. Serial titers measured included antibodies to Epstein-Barr virus (EBV) antigens, group B coxsackieviruses, rubella virus, cytomegalovirus, and herpes simplex virus. Serological evidence of active EBV infection was found in four of the patients with acute arthritis, none of the patients with chronic arthritis, and one of the ten healthy adults. There was no similar correlation between acute disease and presence of antibodies to the other viruses tested. We suggest that EBV may cause acute rheumatic illnesses more commonly than is currently appreciated but is probably not involved in the etiology of typical chronic rheumatoid arthritis. | |
| 6675351 | Studies of polyarthritis caused by mycoplasma arthritidis in rats. III. Histopathological | 1983 May | Mycoplasma arthritidis induced polyarthritis in the rat is characterized histologically by four distinct phases. Firstly, two days after infection, early lesions such as vascular changes and alterations in the lining cell layer of synovium are observed. This is followed four days after inoculation by an acute exudative phase with the deposit of fibrin and infiltration by polymorphonuclear granulocytes together with first signs of joint destruction by pannus-like granulation tissue. From about the third week onwards, lymphocytes and plasma cells predominate in the subsynovium, indicating subacute inflammation. In the fourth phase which lasts until the end of the one year observation period, chronic arthritis with destruction of cartilage and bone, ankylosis, and chronic inflammation, sometimes with acute recurrences, were observed in about 25% of the joints. Both the marked tendency to pannus formation and the longevity of the arthritis, make this an interesting, experimental model of chronic polyarthritis, particularly as it has been induced by a peptidoglycan-free microorganism. | |
| 4138924 | Analyses of lymphocytes from patients with rheumatoid arthritis and systemic lupus erythem | 1974 Nov | Large percentages of the lymphocytes from some patients with rheumatoid arthritis and systemic lupus erythematosus were densely covered with Ig demonstrable by immunofluorescence, which was occasionally present in the form of caps. The amount and character of the Ig staining depended largely on the procedures used in the isolation and washing of the lymphocytes. Cold-reactive antilymphocyte antibodies present in many sera wre primarily responsible for these variations. Overnight culture of the lymphocytes proved to be an efficient procedure for the removal of adsorbed antibody. Some evidence was also obtained for the presence of circulating immune complexes and exogenous rheumatoid factor molecules on the lymphocyte surface. Thus on freshly isolated cells the demonstration of surface Ig proved to an unreliable marker of bone marrow-derived (B) cells in these disease: the actual percent of B cells with intrinsic surface Ig was often markedly decreased. In patients with systemic lupus erythematosus, this reduction was in agreement with the low numbers of cells that had a receptor for aggregated IgG. The mean percentage of thymus-derived (T) cells in both diseases was slightly greater than the normal level.The concentrations of lymphocytes in joint fluids from patients with rheumatoid arthritis were often greater than levels found in blood. T cells primarily accounted for this increase. The T cells typically formed unusually dense rosettes with sheep erythrocytes. B lymphocytes were proportionally much diminished. Evidence was obtained for the existence of a major joint fluid lymphocyte population that lacked all assayed surface markers. | |
| 6813472 | The mechanisms of action of conventional chrysotherapy. | 1982 Jul | The discovery of chrysotherapy occurred 90 years ago. Gold salts (GS) as an antibacterial agent were used initially for tuberculosis (TB), but an infectious etiology was also suspected for rheumatoid arthritis (RA). Patients who received GS for TB and who also had RA noted improvement of their arthritis. The injectable GS regimen now commonly used evolved from the empirical use of GS for RA from 1920-1960. Originally, clinical experience perpetuated the use of injectable GS to modify RA and its progression. However, scientific data obtained in the last 2 decades have supported the efficacy of chrysotherapy. Studies of protein binding, blood and serum concentrations, tissue distribution, and the toxicity of GS all provided suggestions for its mode of action. As chemical and cellular mediators of inflammation were uncovered, the possible actions of gold have been refined. Because the immune system seems to perpetuate rheumatoid inflammation, the interactions with GS continue to be studied. The in vitro and in vivo actions of GS with the immune system so far have provided conflicting data for its immunological effect. Studies by models of mucosal immunity may lead to new insights in the mode of action for gold. | |
| 4004971 | The immunoreactivity, ligand, and cell binding characteristics of rheumatoid synovial flui | 1985 Jun | Fibronectin promotes macrophage adherence and expression of Fc receptors, is chemotactic for fibroblasts, and is an opsonin for fibrin and denatured collagen. These properties suggest a role for fibronectin in the modulation of joint inflammation. Since structural modification of the fibronectin molecule has been shown to result in loss or de novo acquisition of opsonic and chemotactic activity, we determined the functional and immunochemical properties of fibronectin isolated from the inflamed joint. Eighty-six percent of synovial fluids obtained from patients with active rheumatoid arthritis (RA) contained fibronectin fragments, and 39% of the fluids no longer displayed the dimeric form. Compared with native fibronectin, RA peptides were as active in promoting synoviocyte chemotaxis and in glycosaminoglycan binding, but displayed lower affinity for fibrin and gelatin. Although comparable with intact protein in augmenting monocyte attachment to gelatin, the RA synovial fluid peptides did not augment monocyte attachment to fibrin. Analysis of whole synovial fluid and isolated fibronectins by enzyme immunoassay showed that the increased fibronectin immunoreactivity, previously reported in RA synovial fluid, measures intact and nearly intact protein and does not measure extensively degraded fragments. | |
| 144507 | Clinical evaluation of D-penicillamine by multicentric double-blind comparative study in c | 1977 Nov | In order to evaluate clinical efficacy of D-penicillamine (DP) a double-blind study was conducted by the Metalcaptase Research Group consisting of forty-one rheumatological centers in Japan. A total of 179 patients with rheumatoid arthritis (RA) was divided into two groups; one treated with 5 mg (control group) and the other with 100 mg (drug group) of DP in capsule form. The trial lasted 24 weeks. Global judgment by physicians revealed that improvement was found in 27% in the controls and 65% in the drug group. Adverse reactions occurred in 34% of the controls and 49% of the drug group. Skin rashes, taste disturbances, gastrointestinal upset and proteinuria were frequent in the drug group, but severe or fatal reactions could not be seen in this trial. | |
| 1027236 | [Glycosaminoglycans of normal and pathologically altered articular cartilage]. | 1976 Jul | Glycosaminoglycans of tegmental cartilage of human caput femoris were separated into the fractions, which were identified as keratosulfate, hyaluronic acid, heparitin sulphate, chondroitin-4-sulphate, chondroitin-6-sulphate, dermatan sulphate and heparin. Content of glycosaminoglycans in articular cartilage of healthy persons and patients with deforming arthrosis was determined. Content of sulphatated polysaccharides (dermatan sulphate, chondroitin-6-sulphate, keratosulfate and heparitin sulphate) was shown to be distinctly decreased in the cartilage of the patients as compared with the amount of the polysaccharides in the same tissue of healthy persons. | |
| 4421738 | Simple analgesics for arthritis. | 1974 Oct 26 | A series of experiments has been carried out with single doses of simple analgesics in patients with rheumatoid arthritis using a consistent polyad design. This method proved to be both valid and useful. Pain relief scores were a better measure of the effectiveness of analgesics than preference.Aspirin, Codis, and Distalgesic were the most effective analgesics tested, with paracetamol, pentazocine, and Ciba 44,328 intermediate between these agents and placebo. Placebo given after an active analgesic was more effective than when given before; this phenomenon was not abolished by telling the patients that apparently identical tablets were, in fact, different or by making them different in colour. The effectiveness of soluble placebo depended on its colour, red being the most effective. | |
| 84676 | Detection of intermediate complexes by evaluation of the difference between gamma-globulin | 1979 Feb | A simple analysis based on techniques readily available in the routine clinical laboratory has been presented for identification of patients with moderate and high levels of intermediate complexes. If the gamma-globulin concentration determined by serum protein electrophoresis minus the IgG concentration determined by radial immunodiffusion exceeds 1.0 gm/dl, intermediate complexes of IgG should be present in concentrations greater than 0.5 gm/dl. In principle, any disease association with sufficient polymerization of IgG might cause an abnormal result with this analysis. Furthermore, sera from patients with liver disease and marked beta-gamma-bridging on electrophoresis may also give abnormal values. | |
| 428250 | Introduction of antifolics in psoriasis. A twenty-five year retrospect of antineoplastic a | 1979 Apr | Presented herein is a review of the beneficial use of the folic acid antagonist, aminopterin, in the treatment of psoriasis and other dermatologic disorders. | |
| 7017903 | Spontaneous release of leucocyte migration-inhibitory factor by mononuclear cells eluted f | 1980 | Spontaneous leucocyte migration-inhibitory factor (LIF) was assayed by an indirect system in which mononuclear cells (MNC) eluted from rheumatoid synovial tissues or isolated from peripheral blood of normal donors were cultured without antigen. The supernatants from these cultures and control supernatants, heated to 80 degrees C for 30 min to destroy the LIF activity, were used in the test. In seven out of eleven rheumatoid arthritis patients the eluted synovial MNC produced LIF spontaneously, whereas none of the twelve normal blood donors showed any such production. The ability to show spontaneous LIF production was primarily seen in the joint tissues from sero-negative patients. | |
| 758920 | PHA-induced cellular cytotoxicity. Inhibition by a nonimmunoglobulin factor present in ser | 1979 Jan | Phytohemagglutinin-induced cellular cytotoxicity by normal mononuclear cells (MNC) is inhibited by serum of patients with active untreated systemic lupus erythematosus (SLE) but not by that of patients with untreated inactive SLE or rheumatoid arthritis. Three-hour preincubation of normal MNC with SLE sera suffices to inhibit throughout the assay. Preincubation with phytohemagglutinin (PHA) before addition of sera does not prevent the inhibition. DEAE-cellulose chromatography, Sephadex G-200 fractionation and immunoelectrophoretic analysis have shown the nonimmunoglobulin nature of this factor. It is thermostable and not affected by deoxyribonuclease or ribonuclease treatment. The mitogen-induced cellular cytotoxicity-inhibiting factor seems to be independent of other serum factors capable of blocking PHA-induced blastogenic transformation. |