Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 7050390 | Age dependence of spontaneous plaque forming cells in human peripheral blood in Graves dis | 1982 Jun | A protein A plaque forming cell (PFC) assay has been used to assess immunoglobulin synthesis by peripheral blood lymphocytes (PBL) from normal subjects and patients with Graves' disease and rheumatoid arthritis. In normal subjects the number of PFC detected immediately after lymphocyte isolation showed a marked decrease with increasing age. In addition, PBL from patients with Graves' disease or rheumatoid arthritis contained similar numbers of PFC to age-matched control subjects. These findings suggest that patients with these immunological disorders do not show polyclonal B cell activation. | |
| 321815 | Silicone lymphadenopathy and synovitis. Complications of silicone elastomer finger joint p | 1977 Apr 4 | We report two complications of silicone elastomer finger joint prostheses. In one patient, the prostheses broke, with silicone particles present in synovium ("detritic synovitis"). In another patient, silicone particles were found in an axillary lymph node five years after insertion of prostheses in the ipsilateral hand (prostheses were intact at the time). Microscopically, silicone particles in synovium and lymph node were identical to particles abraded from a new prosthesis. | |
| 6908792 | [Activated C3 in the synovial fluid of patients with rheumatoid arthritis and arthrosis (c | 1981 Jan | Cleavage products of C3 in synovial fluid were determined by the method of 2-dimensional electrophoresis (Laurell). Statistically significant differences were observed in rheumatoid synovial fluid compared with the osteoarthrosis group. Storage is of crucial importance for the in vitro activation of C3. In fresh synovial fluid there was a significant positive correlation between breakdown products of C3 and C3, C4 and C3-proactivator. After storage (even in -70 degrees C) this positive correlation in the rheumatoid synovial fluid changes into a negative one. This phenomenon is not observed in synovial fluid from patients with osteoarthrosis. There is evidence that in the rheumatoid synovial fluid C3-activating fractions in 40S, 19S and 2,5S are responsible for the generation of C3 cleavage products. | |
| 6384506 | Immune deposits in the skin of patients with rheumatoid arthritis. | 1984 Aug | The presence of immunoglobulin and complement deposits in cutaneous blood vessels and at the dermal junction was determined in 34 patients with rheumatoid arthritis (RA). Deposits of IgM and C3 were twice as common in the leg than the arm. The deposits were present in 7/14 patients with extraarticular disease and 1/20 patients with articular disease alone. Deposits of IgM were detected at the dermoepidermal junction in 4 patients with RA. All had circulating antinuclear antibodies. | |
| 6610932 | Quantitative and qualitative impairment of immunoregulatory cells in the circulation of rh | 1984 | T cells bearing receptors for the Fc portion of IgM or IgG, TM or TG cells respectively, in the peripheral blood of RA and OA patients were found significantly less often when compared with TM and TG cells in the peripheral blood of normal donors. Using culture combinations of TM, TG and B cells, we were able to demonstrate that the B cells in the peripheral blood of RA patients were constantly of low functional competence and the TMcells varied between highly competent and low competent. RATG cells in general suppressed normal B-lymphoblast differentiation. | |
| 230491 | Antibodies to Epstein-Barr virus-determined antigens in normal subjects and in patients wi | 1979 Nov | Prior studies have shown that patients with seropositive rheumatoid arthritis (RA) have an increased frequency of precipitating antibody against a nuclear antigen, the RA nuclear antigen, detected in human B lymphoblastoid cell lines infected by Epstein-Barr virus. The present investigations demonstrate that patients with seropositive RA also have specifically elevated titers of antibodies to another, better-characterized Epstein-Barr virus-associated B cell antigen, the Epstein-Barr nuclear antigen, which is detected by anti-complement immunofluorescence. Titers of these two antibodies were not affected by absorption of rheumatoid factor from serum. Furthermore, patients with RA did not have elevated titers of antibodies against the Epstein-Barr virus capsid antigen or to three other species of human herpesviruses: herpes simplex type 1, varicella-zoster virus, and cytomegalovirus. In both normal individuals and RA patients there was a significant association between the presence of antibodies to RA nuclear antigen and the titers of antibody to Epstein-Barr nuclear antigen. Thus, normal subjects with antibody to RA nuclear antigen had titers of antibody to Epstein-Barr nuclear antigen equivalent to those of patients with RA and significantly higher than normal subjects lacking antibody to RA nuclear antigen. One interpretation of these results is that patients with seropositive RA derive from a larger population with enhanced immune responsiveness to B lymphocyte nuclear antigens determined by the Epstein-Barr virus. | |
| 6318777 | Lack of activation of C1, despite circulating immune complexes detected by two C1q methods | 1984 Jan | The activation of C1 by circulating immune complexes in patients with rheumatoid arthritis was investigated. C1rC1s(C1-In)2 complexes in EDTA-plasma, reflecting C1 activation in vivo, were slightly raised in 35 of 57 patients with rheumatoid arthritis, though most patients had elevated levels of circulating immune complexes as measured with either the 125I-C1q binding test or the C1q solid phase assay. The activation of C1 by circulating immune complexes in vitro was investigated by measuring the generation of C1rC1s(C1-In)2 complexes during 60 minutes at 37 degrees C in diluted recalcified EDTA-plasma. In 16 of the 57 patients, a slightly increased C1 activation in vitro was observed. These patients tended to have high levels of circulating immune complexes. However, the majority of the patients with high levels of circulating immune complexes showed a normal C1 activation in vitro. Therefore, it was concluded that measurement of circulating immune complexes by either of the two C1q methods in patients with rheumatoid arthritis does not imply that these circulating immune complexes are able to activate C1. | |
| 6895909 | A comparative study on the rate of de-esterification of dexamethasone phosphate and dexame | 1981 Nov | Considering the importance of the 21-OH group in promoting the anti-inflammatory activity of the steroids, it is possible that differences in the rate of hydrolysis of steroid esters may influence the potency of pharmacological activity of the steroids (Polley and Mason, 1950, Goldfien, et al., 1955). In the present paper the rate of de-esterification of the steroid esters in human synovial fluids was studied in vitro. Hydrolytic rates of dexamethasone-21-sulfate (DS) and dexamethasone-21-phosphate (DP) in synovial fluids aspirated from the knee joint of 10 patients each with rheumatoid arthritis and osteoarthritis of the knee measured by radioimmunoassay. DP was hydrolyzed to the extent of 10%, 30%, 50%, 80% after incubation for 1, 3, 6, 24 hours respectively. On the other hand, DS was hydrolyzed to the extent of less than 10% even after incubation for 24 hours. The difference between the rate of de-esterification of DP and DS for each period was statistically highly significant (p less than 0.001). These results suggest that DP is hydrolyzed in synovial fluids much faster than DS and converted into free dexamethasone which may exhibit a powerful anti-inflammatory action. | |
| 6772541 | Acute nonlymphocytic leukemia in patients receiving chemotherapy for nonmalignant diseases | 1980 Mar | The occurrence of acute leukemia in patients receiving chemotherapeutic agents for malignant disease has been well established. Recent reports have suggested that chemotherapeutic drugs used to treat inflammatory conditions may have an oncogenic potential. From 1969 to 1977, 11 patients with a variety of collagen-vascular diseases who developed acute nonlymphocytic leukemia were seen at the Cleveland Clinic. Rheumatoid arthritis was the most common underlying disease, in addition to giant cell arteritis, polyarteritis nodosa, chronic glomerulonephritis, and scleroderma. All patients were treated with alkylating agents, and 10 of the 11 received multiple cytotoxic agents. According to the French-American-British classification there were six examples of M4 (myelomonocytic leukemia), with single examples of M1 (myeloblastic leukemia without maturation), M2 (myeloblastic leukemia with maturation), M5a (monocytic leukemia, poorly differentiated), M5b (monocytic leukemia, differentiated), and M6 (erythroleukemia). Cytogenetic studies were abnormal in five patients studied, showing varying degrees of aneuploidy. All patients died, and the mean duration of time from the diagnosis of leukemia to death was four and one-half months, with only one complete remission. | |
| 220878 | Peripheral rheumatoid ulceration and evidence for conjunctival collagenase production. | 1979 May | Two patients with rheumatoid arthritis and peripheral corneal ulcerations were successfully treated by conjunctival resection. The tissues removed were assayed by a variation of the radial diffusion method for tissue collagenases. We used an agarose matrix containing lathyritic rat skin collagen. Wells 3 mm deep were punched in the agarose-collagen and surgical specimens were placed in the wells. Spaces remaining in the wells were filled with balanced salt solution. The assay dishes were incubated for four days at 32 degrees C near 100% humidity. Under these conditions release of collagenase was detected by the clearing of diffuse zones in the gel surrounding the well. Conjunctiva proximal to the ulcer produced definite zones of lysis, whereas control specimens taken remote to the ulceration produced no lysis. This direct evidence for collagenase involvement offers an exploration for the beneficial effects of conjunctival resection. | |
| 7444304 | [Rheumatoid pleurisy. Apropos of 13 cases]. | 1980 Nov | The extra-articular manifestations of RA are numerous and various. Among the latter, pleural involvement is not exceptional, but it is often overlooked for it may be latent. There have been few recent studies of this relationship. The authors report 13 cases of rheumatoid pleurisy collected over the last 7 years in rheumatology and chest units. They recall the frequency of pleurisy, their constitutional background, their clinical and radiological appearances and emphasise the diagnostic interest of a biochemical study of the pleural fluid: a fall in the glucose content and of complement levels are the main changes. A study of the complement system carried out in 11 patients showed a fall in total complement and of the C3 and C4 fractions in the pleural fluid, which shows local consumption of immunological origin. This factor and the clinical picture may be important in diagnosis. The course of these pleural effusions is favourable, complications are exceptional; their treatment is mainly the basic treatment of RA. | |
| 4082355 | High tibial osteotomy in degenerate diseases of the knee. | 1985 Apr | Between 1970 and 1981, 64 patients underwent 77 tibial osteotomies for degenerate diseases of the knee at the Withers Orthopaedic Centre in Belfast. Records on 11 patients (12 knees) were either missing or inadequate, leaving 53 patients who underwent 65 tibial osteotomies for study. There were 23 males and 30 females, ranging in age from 23 to 75 years (mean 59.8 years). The predominant diagnosis was osteoarthrosis and the indication for operation in all cases was pain. With a follow-up of from two to ten years (mean 4.8 years), 39 knees were assessed as good, 15 as fair (improved, but still symptomatic), and 11 as failures. Patients with valgus deformity did worse than those with varus deformity. The importance of adequate pre-operative assessment is stressed, the operation itself is outlined, and the end result is seen to correlate closely with the degree of correction obtained. | |
| 6849750 | Protein binding of non-steroidal anti-inflammatory drugs in plasma and synovial fluid of a | 1983 Jan | 1 The protein binding of seven non-steroidal anti-inflammatory drugs (indomethacin, tolmetin, salicylic acid, ibuprofen, flurbiprofen, naproxen and GP53,633) and warfarin was investigated by equilibrium dialysis in simultaneous samples of synovial fluid and plasma from 12 arthritic patients. 2 The protein binding of all drugs studied except warfarin and flurbiprofen was significantly lower in synovial fluid than in plasma. 3 The decreased protein binding of these drugs is likely to explain the lower total drug concentrations found in synovial fluid in comparison to plasma. 4 The lower albumin concentration plays an important role in determination of reduced drug binding in synovial fluid compared to plasma and the fatty acid concentration in synovial fluid may also influence the protein binding of some of these drugs. | |
| 6293970 | In vitro induction of anti-intermediate filament antibody in lymphocyte cultures by Epstei | 1982 Oct | Serum antibodies reactive with intermediate filaments of the cytoskeleton (anti-IF antibodies) are often present in infectious mononucleosis, some other viral diseases, and rheumatoid arthritis. The mechanism of their production is not known, but it is possible that the formation of this and other autoantibodies result from polyclonal activation of B-cells. Peripheral blood mononuclear cells from subjects with or without serum anti-IF antibody were therefore cultured in the presence or absence of Epstein-Barr virus (EBV). IgM anti-IF antibody was produced in both unfractionated and T-cell-depleted cultures, but not in the supernatants of the same cells cultured without added EBV. | |
| 7039524 | Antibody to intermediate filaments of the cytoskeleton. | 1982 Feb | IgM antibodies against cultures of intermediate filaments (IMF) of the cytoskeleton were demonstrated by immunofluorescence in the sera of 94 (80%) of 118 patients with seropositive rheumatoid arthritis. These antibodies reacted with IMF in cultures of both human fetal fibroblasts and laryngeal carcinoma (HEp2) cells. Of 10 patients from whom paired synovial fluids were also available 8 had anti-IMF antibodies in both serum and fluid. In seronegative RA the incidence of anti-IMF was 40%, in ankylosing spondylitis 25%, in osteoarthrosis 16%, and in normal subjects 14%. Only a minority of RA sera positive for anti-IMF antibodies were also positive for smooth muscle antibody. Absorption experiments suggest that in RA anti-IMF is directed at the intermediate filament protein, vimentin. | |
| 6302818 | B-lymphocyte subpopulation which forms rosettes with mouse erythrocytes increased in rheum | 1982 | We studied the peripheral blood lymphocytes of 22 patients with rheumatoid arthritis (RA) for the presence of a subpopulation of cells which form rosettes with mouse erythrocytes. In normal subjects these cells have been characterised as immature B cells which are non-responsive to pokeweed mitogen. The mean percentage of mouse rosette-forming cells (MRFC) in the rheumatoid group was 13 +/- 10(mean +/- 2 SD), a significantly higher value than the control mean of 5% +/- 4% (P less than 0.001). The T- and B-cell percentages in the rheumatoid patients were normal. The ratio of MRFC: B cells derived from these results was 3:4 in RA and 1:4 in normal subjects. Pre-incubation of rheumatoid peripheral blood lymphocytes at 4 degrees C gave higher values of MRFC (19% +/- 10%) than pre-incubation at 37 degrees C (13% +/- 10%, P less than 0.02), but no such temperature effect was found in the control group. There was no correlation between MRFC and rheumatoid disease activity or the patients' drug regimens. We conclude that the threefold increase in mean MRFC in patients with rheumatoid arthritis indicates an abnormality in the circulating B-cell pool. | |
| 7019873 | Immunologic tests of value in diagnosis. 2. Complement. | 1981 Aug | Laboratory tests are available to assess the function of the complement pathway and to measure levels of individual complement components. The pattern of complement abnormalities is often helpful in suggesting diagnostic possibilities. For example, when when total hemolytic complement, C3, and C4 are all decreased, one of the rheumatoid diseases is likely. In addition, complement levels in spinal and synovial fluid may provide helpful diagnostic clues. | |
| 426883 | Steady-state serum salicylate levels in hospitalized patients with rheumatoid arthritis. C | 1979 Apr | When the total daily drug dose was individualized to produce a steady-state serum salicylate concentration between 20 and 35 mg/dl, clinically acceptable fluctuations of serum concentrations occurred during both twice daily and three times daily administration. In 6 rheumatoid arthritis patients receiving choline magnesium trisalicylate, mean steady-state serum levels were the same, and the ranges of hourly mean concentrations during 8 and 12 hour dosage intervals were 19 to 27 mg/dl and 17 to 30 mg/dl, respectively. Changing the dosing interval from 8 to 12 hours required a 50% increase in the fractional doses, but resulted in an increase of only 3 mg/dl in mean peak concentration and a ddecrease of 1 mg/dl in mean minimum concentration. | |
| 6681140 | Protease inhibitors in rheumatoid synovial fluid: a quantitative analysis. | 1983 Jul | The concentrations of the main endogenous inhibitors of granulocyte proteases (anti-leukoprotease, alpha 1-antitrypsin, alpha 1-antichymotrypsin, and alpha 2-macroglobulin) were estimated in paired samples of synovial fluid and serum/plasma from seropositive rheumatoid arthritics and controls. Rheumatoid synovial fluid contained significantly higher levels of all inhibitors except antileukoprotease. The influence of the synovial membrane on these concentrations was taken into account by comparing the ratio between the observed concentration and that predicted from a certain regression curve fitted to a set of non-inhibitory reference proteins of extra-articular origin (orosomucoid, albumin, and ceruloplasmin). Divergences were interpreted as the net result of intra-articular production or consumption of the inhibitor in question. The results suggested a consumption of antileukoprotease and alpha 1-antitrypsin in the rheumatoid joint, while the increased levels of alpha 1-antichymotrypsin and alpha 2-macroglobulin probably reflected the altered trans-synovial membrane protein flux with some reservation for alpha 2-macroglobulin. | |
| 6895043 | The synovial prostaglandin system in chronic inflammatory arthritis: differential effects | 1981 Aug | 1 The present study was undertaken to characterize the spectrum of arachidonic acid metabolites present in synovial effusions of patients with rheumatoid or psoriatic arthritis, and to compare changes in their concentration following a short-term treatment with 6alpha-methyl-prednisolone (6-MeP: 4-8 mg/day) or indoprofen (1.2 g/day), a nonsteroidal anti-inflammatory agent with proven synovial prostaglandin inhibitory effect.2 Measurements of prostaglandin E(2) (PGE(2)), thromboxane (TX) B(2), 6-keto-PGF(1alpha) and PGF(2alpha) were performed by radioimmunoassay techniques in synovial effusions obtained from 23 patients, and validated by thin-layer chromatographic analysis of the extracted immunoreactivity.3 PGE(2) and TXB(2) accounted for more than 60% of the total immunoreactivity in untreated patients. The absence of any constant ratio between the different arachidonic acid metabolites detected in synovial fluid is consistent with a heterogeneous cellular origin of these compounds.4 Indoprofen treatment was associated with a consistent reduction of synovial prostaglandin and thromboxane concentrations, ranging from 36% in the case of 6-keto-PGF(1alpha) to 90% in the case of PGE(2).5 In contrast, 6-MeP caused opposite changes on different metabolites originating via the cyclo-oxygenase pathway. Thus, 6-keto-PGF(1alpha) concentrations were reduced by 35%, PGF(2alpha) concentrations were increased by 30%, while PGE(2) and TXB(2) were unchanged following 6-MeP.6 Although the mechanism(s) underlying the failure of 6-MeP to reduce synovial PGE(2) and TXB(2) levels are uncertain, the results of the present study clearly indicate that therapeutic doses of steroidal and nonsteroidal anti-inflammatory drugs cause quite distinct changes in arachidonic acid metabolism, which might be relevant to their specific therapeutic actions and side-effects. |