Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 6721882 | Human IgG aggregates induce selective stimulation of IgM rheumatoid factor synthesis by rh | 1984 May | Peripheral blood mononuclear cells (PBM) from patients with active rheumatoid arthritis (RA) contain precursor B lymphocytes specific for IgM rheumatoid factor (IgM-RF) synthesis. In this study, human IgG aggregates (HaIgG) were used to stimulate monocyte-depleted PBM from 46 patients with RA and 21 normal controls. The cells were incubated with HaIgG and pokeweed mitogen for 72 hours, washed, and then cultured in microwells for an additional 11 days. HaIgG induced an increase in IgM-RF synthesis by RA cells with optimum response at 0.1 microgram/ml (P less than 0.001). Total IgM synthesis remained unchanged. In contrast, HaIgG did not stimulate IgM-RF production by normal cells. Specificity of the IgM-RF response was shown by the concomitantly increased IgM-RF/IgM ratios, while IgM anti-trinitrophenyl antibodies did not increase. Aggregation of the IgG was required for it to be an effective stimulus. The findings suggest that circulating immune complexes in RA patients may provide the stimulus for sustained production of IgM-RF in vivo. | |
| 770084 | Prolonged treatment with azapropazone. | 1976 | In an open study of 40 patients with chronic rheumatoid polyarthritis, azapropazone was given alone in daily doses between 1200 mg. and 1800 mg. over a period of 6 months. Six patients were withdrawn, 2 because of side-effects (1 with an allergic-type rash; 1 with ankle oedema) and 4 because azapropazone alone did not provide adequate control of their pain and discomfort. Objective assessments showed that 18 patients had a 'good' or 'very good' response to treatment, and a further 16 showed moderate improvement. These findings were supported by the patients' subjective assessment of the change in their condition from the start of the trial. In addition, 22 patients showed a significant decrease in erythrocyte sedimentation rate. There was no evidence of adverse effects on any of the haematological and biochemical parameters measured, and the incidence of side-effects was low. | |
| 1020400 | [Screening of collagen diseases in the years 1961-1972 from a selected population sample]. | 1976 Dec 1 | In our article is referred to the recognition of collagenosis in the demarcated whole district with 100,000 inhabitants. The occurrence of these diseases is lower than the occurrence of other rheumatic diseases. Our results as well as the observations of other authors confirm that the lupus erythematodes and the diffuse sclerodermia are not rare diseases even in our circumstances and with their evolution tendency they shorten the lives of the affected persons. The high recognition coefficient with large probability corresponds to the real incidence of these two diseases, which can be achieved only by an observation of many years and repeated controls of the correctness of the diagnosis. | |
| 773034 | [Evaluation of phenylalkanoic acid derivatives in the management of rheumatic diseases as | 1975 Jan | The pharmacology and toxicology of the phenylalkanoic derivatives are discussed with particular reference to Ibuprofen. Clinical trials of Ibuprofen are reviewed and their methodology assessed. A more rational approach to anti-inflammatory drug evaluation is suggested and the need for closer international co-operation in establishing a standardized approach to clinical trial methodology is emphasised. | |
| 7255126 | [D-penicillamine induced myasthenic syndromes in rheumatoid arthritis. Two cases (author's | 1981 May 2 | The authors report on 2 personal cases, and review 48 published cases of myasthenia induced by D-penicillamine (D-P) treatment in patients with rheumatoid arthritis. The clinical symptoms were not different from those of myasthenia gravis, and no correlation could be found between the total cumulative dose of D-P and the onset on the myasthenic syndrome. In 71% of the patients the neurological deficiency regressed after D-P was withdrawn, but in some cases anticholinesterase treatment had to be continued and thymectomy was contemplated. The most remarkable biological abnormalities were anti-striational antibodies (found in 58% of the cases) and anti-acetylcholine receptors antibodies (found in 4 out of 7 patients tested). These findings are in favour of a genuine myasthenia and against a myasthenic syndrome due to neuro-muscular blockade. While the mechanisms underlying the emergence of these antibodies remains unknown, their presence throws new light on immunological disorders in rheumatoid arthritis. | |
| 6758110 | Quantitation of cells secreting immunoglobulins after elution from rheumatoid synovial tis | 1982 Nov | Mononuclear cells (MNC) eluted from rheumatoid synovial tissue of 16 patients with rheumatoid arthritis were examined for immunoglobulin-secreting cells (ISC). Immediately after elution and separation synovial tissue MNC contained considerable numbers of ISC. IgG and IgA ISC were more abundant than IgM ISC. At the same time there were low numbers of ISC in blood. Synovial tissue ISC were lost during incubation with pokeweek mitogen (PWM), possibly because tissue MNC were already maximally triggered in vivo. This was in contrast to blood MNC, in which the number of ISC increased significantly during incubation with PWM. | |
| 312088 | Immunological characteristics of lymphocytes in synovial fluid and peripheral blood in pat | 1978 | The investigation has been carried out on 30 patients with rheumatoid arthritis. Rosettes (E, EA, EAC) technique and immunofluorescence test were used to detect the surface markers of lymphocytes. Cytotoxic activity of T and K cells in vitro was established. It has been proved that cells occurred to a larger extend in synovial fluid than in the peripheral blood. B cells and lymphocytes with Fc receptors were only a few in synovial fluid. The percentage ofFc receptors bearing cells was higher in the peripheral blood of patients with rheumatoid arthritis than that in peripheral blood of the control group. Cytotoxic activity of K-cell was low in synovial fluid. PHA-cell mediated cytotoxicity was low in peripheral blood as well as in synovial fluid of patients with rheumatoid arthritis. | |
| 219787 | Mitochondrial oxidative activity in human rheumatoid synovial lining cells. | 1978 Dec | The activities of two mitochondrial enzymes, succinate dehydrogenase and cytochrome oxidase, have been measured by quantitative cytochemistry and microdensitometry in the synoviocytes of rheumatoid and non-rheumatoid synovial lining cells. Although both tended to be higher in the former, there was no statistically significant difference in the activities of either enzyme in these tissues. However, when cytochrome oxidase activity was measured without exogenous cytochrome c, the activity in the rheumatoid synoviocytes was highly significantly elevated. It is suggested that these findings may indicate only that the cytochrome c-cytochrome oxidase complex in the rheumatoid cells is more stable, possibly because of the increased availability of phospholipids in these cells. | |
| 6715830 | Proplast stabilized stemless trapezium implant. | 1984 Mar | Forty-five stemless Proplast-covered silicone rubber trapezium implants were used in 40 patients after trapeziectomy for either osteoarthritis, rheumatoid arthritis, or trauma. Three implants were later removed from two patients because of traumatic dislocations. One implant was removed from a patient after trauma because of persistent pain. Five patients were followed from 40 to 44 months; nine patients were followed from 31 to 39 months; 15 patients were followed from 20 to 29 months; six patients were followed from 14 to 19 months; five patients were followed from 6 to 9 months. The average follow-up time was 24 7/10 months. Before surgery 5% of the patients had continuous spontaneous pain, 35% of the patients had pain with all motion, and 60% of the patients had pain with limited normal daily use. At this time of our report, 65% of the patients are without symptoms, 21% of the patients have slight intermittent pain, and 14% of the patients have pain with strenuous use only. Tripod pinch improved 243% in male patients and 113% in female patients. All patients have good circumduction and metacarpophalangeal (MP) and interphalangeal (IP) joint motion. The stemless feature permits relatively free metacarpal joint motion without great stress being applied to the capsule that is covering the implant. | |
| 4057484 | Remitting seronegative symmetrical synovitis with pitting edema. RS3PE syndrome. | 1985 Nov 15 | Eight elderly men and two elderly women presented with symmetrical polysynovitis of acute onset involving most of their appendicular joints and flexor digitorum tendons associated with pitting edema of the dorsum of both hands and both feet. Onset of seven of the ten cases could be pinpointed almost to the hour. Rheumatoid factors were absent from serum samples in all, and no radiologically evident erosions developed. Clinical and laboratory signs of inflammation and the edema disappeared gradually in each case. Treatment consisted of aspirin or other nonsteroidal anti-inflammatory drugs. Hydroxychloroquine, 200 to 400 mg/day, was given in six and gold therapy in two cases. Painless limitation of motion of the wrists and/or fingers persisted in all, although the patients were both unaware of and unhampered by this abnormality. Six of eight cases where typing was possible were positive for HLA-B7, CW7, and DQW2 (relative risk for B7, 9.5). Three cases of this syndrome were found in a consecutive series of 52 men diagnosed as having definite "rheumatoid arthritis," and thus represent a distinctive condition with an excellent prognosis. | |
| 6346800 | Secretion of IgM-rheumatoid factor and IgM by blood lymphocytes in rheumatoid arthritis. | 1983 Feb | A direct plaque forming cell (PFC) assay for the detection of complement fixing IgM-rheumatoid factor (RF) secreting lymphocytes was evaluated. The specificity of the RF-PFC assay was demonstrated by the inhibitory action of exogenous human IgG. The sensitivity of the RF-PFC assay was similar to that of a reverse PFC assay detecting all cells secreting IgM (IgM-PFC). In blood from 75% of seropositive patients with rheumatoid arthritis (RA) RF-PFC were demonstrated, median value 6.8 (range: 0.4-1477) RF-PFC/10(6) mononuclear cells. Practically no RF-PFC were detected in seronegative RA patients and controls. Despite the fact that most IgM-RF undoubtedly is produced outside the blood, a positive correlation was found between the number of RF-PFC and the Waaler-Rose titer in serum. The number of circulating IgM secreting cells did not differ significantly between seropositive RA patients, seronegative RA patients and controls. Comparison of RF-PFC and IgM-PFC in seropositive patients revealed that in mean 7% of IgM-secreting cells in blood secrete IgM-RF. | |
| 6185339 | beta 2-Microglobulin and beta 2-microglobulin-binding proteins in inflammatory diseases. | 1982 | Beta 2 microglobulin (beta 2m) is a 11,800 daltons polypeptide non covalently associated with the heavy chain of class I histocompatibility antigens (HLA-A, B and C) at the surface of nearly all cells. Serum beta 2m levels are passively controlled by the glomerular filtration rate. Increased beta 2m production resulting in elevated serum levels despite normal renal function have been reported in malignancies of the lymphoreticular system (e.g. multiple myeloma) and in various autoimmune or chronic inflammatory diseases, including rheumatoid arthritis. In some situations beta 2m levels were shown to be positively correlated with absolute lymphocyte counts in the peripheral blood or with the score of mononuclear cell infiltrates in biopsy specimens. Together with the demonstration that activated T lymphocytes release beta 2m in culture, these data support the hypothesis that increased production of beta 2m in vivo could represent a non specific indication of lymphocyte activation. Follow-up studies of individual patients are needed to define the clinical situations in which beta 2m determination may improve the immunological monitoring, with special reference to the early diagnosis of relapses and the assessment of individual response to treatment. | |
| 3880180 | Felty syndrome: autoimmune neutropenia or immune-complex-mediated disease? | 1985 | Immunofluorescence on polymorphonuclear cells (PMN) of patients with Felty syndrome (FS) revealed increased amounts of IgG, IgA, and IgM bound to the PMN surface compared with PMN of patients with rheumatoid arthritis alone. A positive correlation was found between the score for surface-bound immunoglobulins on FS-PMN and the results of the Clq binding assay in FS sera. After preincubation with sera from 20 patients with FS, immunofluorescence on PMN from healthy controls (HC) showed that these cells had bound IgG, IgA, and IgM. However F(ab')2 fragments of IgG from FS sera did not bind to PMN, although the antigen-binding reactivity of the F(ab')2 fragments was maintained as shown by control experiments. Immunoglobulins eluted from FS-PMN failed to bind to HC-PMN, whereas the corresponding IgG of patients with autoimmune neutropenia was bound. Gel filtration of FS sera on Sepharose 4B showed that the binding of IgG in FS sera to PMN did not coincide with the 7S peak but occurred mainly in fractions containing larger material. No binding of IgA and IgM to HC-PMN was found after incubation with FS sera pretreated with polyethylene glycol (PEG) to precipitate immune complexes. These results indicate that in sera of patients with FS the PMN-binding reactivity of IgG, IgA, and IgM is due to the binding of immune complexes containing these immunoglobulins and not to presence of autoantibodies directed to antigens on the neutrophil surface. | |
| 6626699 | Systemic bioavailability and pharmacokinetics of methylprednisolone in patients with rheum | 1983 Jul | The absolute and relative bioavailability of two methylprednisolone formulations (capsules and suspension) was determined along with its pharmacokinetics in four arthritic female patients, following an unconventional high-dose pulse of 1 g. Plasma concentrations of the drug were measured by a sensitive and specific high-performance liquid chromatographic (HPLC) procedure. The disposition of methylprednisolone from plasma following intravenous (i.v.) infusion of its succinate ester appeared monoexponential with a mean half-life of 2.4 h and an apparent volume of distribution (Vd) of 50 l (0.87 l/kg). The total body clearance (Cl) averaged 15.12 l/h. Absolute bioavailability was assessed by comparing the areas under the plasma concentration time curves (normalized to dose) following oral administration of capsule or suspension with those of intravenous administration. No significant difference (p greater than 0.2) was observed when systemic availability (f, expressed in per cent) following administration of drug in capsule (f = 49.35 per cent) was compared with that obtained following the administration of drug in a suspension (f = 58.26 per cent). The difference in the observed and predicted f may be due to incomplete absorption, hepatic and/or extrahepatic metabolism of methylprednisolone. Subjective evaluation showed no side effects of this high-dose pulse therapy in any of the patients. | |
| 7191308 | [Influence of acemetacin on DNA repair and DNA synthesis in vitro and in vivo (author's tr | 1980 | The effects of [1-(p-chlorobenzoyl)-5-methoxy-2-methyl-indole-3-acetoxy]-acetic acid (acemetacin, TV 1322, Rantudil) on DNA repair (unscheduled DNA synthesis) and semiconservative DNA synthesis have been investigated in vitro in mouse spleen cells and in vivo in human leukocytes. Quantitative determination of DNA repair was made possible by a computer program and additional measurement of parameters of uptake kinetics. in vitro results showed no influence on DNA synthesis and therapeutically relevant concentrations had no influence on DNA repair. 6 female patients with diagnosticized rheumatoid arthritis received acemetacin 4 x 30 mg daily for three months. During the duration of treatment there was no significant change in DNA repair capacity or in semiconservative DNA synthesis in comparison to pre-treatment values. | |
| 6751841 | The pathology of small airways in chronic airflow limitation. | 1982 | Small airways' disease is an ambiguous term that has been used in three ways: 1. to describe the lesions in the peripheral airways in patients with severe chronic airflow limitation; 2. to describe patients with isolated severe bronchiolitis and 3. to describe patients with mild chronic airflow limitation or the lesions in the small airways of such objects. Emphysema is probably the most important variable in causing chronic airflow limitation. The lesions in the peripheral airways in patients with severe chronic airflow limitation include narrowing and loss of small airways, inflammation, goblet cell metaplasia and intraluminal mucus. The first two lesions are best related to the development of cor pulmonale. In emphysema the peripheral airways are greatly distorted and lack alveolar support, and stenotic areas decrease more in dimension than the rest of the airways. Isolated severe bronchiolitis is best defined in patients with rheumatoid arthritis and causes a characteristic clinical syndrome of rapidly progressive airflow limitation. The most important cause of mild chronic airflow limitation is inflammation of the peripheral airways. This may act by displacing the surface active layer of the airway or, more likely, by inducing muscular hyperactivity. This may result from a direct action on muscle by mediators or may act through a vagally mediated reflex. | |
| 6978604 | Chronic immune synovitis in rabbits. II. Modulation by anti-inflammatory and anti-rheumati | 1981 Dec | Synovitis was induced in rabbits sensitized to bovine serum albumin (BSA) by a modification of the method of Dumonde and Glynn. Agents were administered starting on the day of initial BSA joint challenge and every weekday (14-17 doses) for 17 to 21 day periods. Synovial tissues were then excised and evaluated either (1) histologically for inflammatory cell infiltration, or (2) both histologically and for total IgG, anti-BSA, and beta-glucuronidase levels in homogenates of portions of the same tissues. By the intraperitoneal and oral routes, methylprednisolone (1 mg/kg/day), azathioprine (25-40 mg/kg/day) and cyclophosphamide (10 mg/kg/day) produced significantly decreases of 40-100% in some or all of the parameters measured. Non-steroidal anti-inflammatory drugs, including phenylbutazone, ibuprofen and acetylsalicylic acid at oral doses of 75 or 100 mg/kg/day, were ineffective as were colchicine at 1 mg/kg/day and indomethacin at 5 mg/kg/day. Thus, as we have measured it, this model of rheumatoid arthritis is not affected by those agents considered to be of limited effectiveness in this chronic disease, but is ameliorated by corticoids and some slow acting agents. | |
| 7394475 | [Idiopathic immunoglobulin deficiency in juveniles and adults. Catamnestic studies]. | 1980 Apr 19 | In 1974 and 1975 7000 patients' sera were tested for levels of IgG, IgA and IgM. In 330 patients at least one of the three Ig classes was low. In most instances secondary immunodeficiency was present in association with myeloma, etc. However, 81 patients above 8 years of age fulfilled the criteria of idiopathic late onset Ig deficiency. In 44 of these patients clinical follow-up and repeated measurements of Ig levels were possible 1--8 years after the initial diagnosis. Selective IgA deficiency was present initially (15 patients) most frequently and persisted most often (14 patients). 4 patients had initially low IgG and 6 patients low IgM, findings which were only rarely confirmed later on. In 19 patients 2 or 3 Ig classes were initially low, with persistence of Ig deficiency in 12 individuals. In no instance had clinical symptoms appeared in the first two years of life. The following diseases were documented in the 44 patients studied (28 individuals with persistent and 16 with transitory Ig deficiency): recurrent infections (16 patients), atopic disease (8 times), rheumatoid arthritis (6 times), epilepsy (4 times), SLE (3 times) and enteropathies (twice). Seven patients also had a malignancy, 4 diabetes, and 2 hyperthyroidism. | |
| 786193 | Treatment of rheumatoid arthritis: a review including newer and experimental anti-inflamma | 1976 Sep | Treatment of rheumatoid arthritis is often frustrating for patient and physician alike because of its chronic nature and our lack of knowledge about precise cause. It can be confused by the vast array of anti-inflammatory drugs now available, with new agents being added all the time. We present here an outline for a systematic treatment program that must be tailored to each patient's needs. It emphasizes a broad approach on the part of the physician and indicates that drugs comprise only one part of treatment. We review the drugs currently used in the treatment of rheumatoid arthritis and include discussion of those newer antiinflammatory agents that should be available shortly. A rational scheme for the use of these drugs is proposed. | |
| 6378209 | Rheumatoid myositis. Clinical and histologic features and possible pathogenesis. | 1984 Jul | Thirty-one patients with rheumatoid arthritis were consecutively studied for evidence of muscle involvement, using muscle biopsy and electromyography. The patients were initially separated into 4 clinical categories: inactive peripheral joint disease (6 patients); active peripheral joint disease (10 patients); systemic disease and a disproportionately elevated sedimentation rate for the degree of mild synovitis (SERD) (11 patients); or elevated creatinine phosphokinase level (4 patients). In addition to routine histology, muscle tissue was examined for de novo synthesis of IgM and IgM rheumatoid factor, and by indirect immunofluorescence for the presence of immunoglobulin and complement deposits. Our results indicate that: muscle fiber necrosis occurs frequently in patients with rheumatoid arthritis; rheumatoid myositis, defined as muscle fiber necrosis and mononuclear cell infiltration, is a distinct entity and occurs particularly in patients with SERD or an elevated creatinine phosphokinase level; and only muscle from patients with rheumatoid myositis exhibited de novo synthesis of rheumatoid factor and significant quantities of IgM, indicating that local immune events may be important in the pathogenesis of this entity. |