Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 374734 | Stimulation of lymphocyte reactivity by a low molecular weight cutaneous antigen in patien | 1979 Jan | A low molecular weight cutaneous antigen was found to stimulate the release of macrophage migration inhibition factor from circulating lymphocytes of patients with diffuse scleroderma. The antigen had a molecular weight of approximately 3,500 and contained RNA and polypeptides, but no hydroxyproline. Lymphocytes from patients with the CREST syndrome, rheumatoid arthritis, and from normal controls did not respond to the antigen. An immune response to this antigen may be a factor in the pathogenesis of diffuse scleroderma. | |
| 87203 | Comparison of three immunoassays for immune complexes in rheumatoid arthritis. | 1979 May | Three widely used radioassays that depend on different principles for the measurement of circulating immune complexes (CIC) in biologic fluids are the monoclonal rheumatoid factor (mRF), Raji cell, and C1q binding tests. A comparison of the ability of these methods to measure immune complex-like material in 71 sera and 30 synovial fluids of 91 patients with rheumatoid arthritis (RA) was carried out by a group working in adjacent laboratories in a single institution. The highest number of abnormal levels in the seropositive group was detected by the C1q binding assay (91%). Levels of CIC by the mRF and Raji cell tests were elevated in 81% and 76% of the patients, respectively. The closest correlation was between the Raji and mRF tests (r = 0.44 and P = 0.002) although one depends on complement fixation and one does not. Though significant correlations between the levels of CIC determined by the C1q test and either the mRF (P = 0.2) or Raji cell (P = 0.3) assay were not found in this group, 59% of the samples had elevated levels by all three tests. The frequency of CIC in the sera of patients with seronegative RA was much lower, with the C1q test again giving the highest number of abnormal results (29% versus 16% and 12% for the Raji and mRF tests). In view of the technical problems associated with these tests, particularly lack of a uniform reliable standard, it is likely that all three tests measure the same material in most RA sera and that some of the differences observed are related to inherent variability in the tests themselves rather than intrinsic differences among the CIC detected in these samples. | |
| 7040465 | Evaluation of fluorescent antinuclear antibody assays, Crithidia luciliae substrate, and s | 1982 Apr | Sera from groups of patient with systemic lupus erythematosus, mixed connective tissue disease, rheumatoid arthritis, and progressive systemic sclerosis and normal controls were compared, using different antinuclear antibody assays. Hep-II cells, used as a substrate for the detection of antinuclear antibodies, appeared to be more sensitive than rat liver substrate. In addition, the fluorescent patterns were easier to identify on Hep-II cells. All systemic lupus erythematosus sera with antibodies reactive with kinetoplasts of Crithidia luciliae had binding greater than 43% for single-stranded DNA. Based on the high sensitivity of the Hep-II substrate and the relative specificity of high (greater than 43%) binding for single stranded DNA by sera from patients with systemic lupus erythematosus, it appears that these two tests are most useful in differential diagnosis and for the detection of systemic lupus erythematosus. | |
| 866906 | Lactoferrin in synovial fluid. | 1977 May | The synovial lactoferrin (LF) concentrations of 59 patients with active rheumatoid arthritis were determined. The median value of LF was 4.64+/-3.59 mg/100 ml, but in degenerative arthropathy the levels of the metal-protein were much lower and often not titratable. These variations in the LF concentration explain the low blood-iron levels in inflammatory states, even when the concentration of the metal-protein is not statistically correlated in inflammatory tests, IgG, complement fractions (of the normal or alternate pathway), or variations of other protein fractions of leucocyte origin. | |
| 6131862 | Vasculitis in primary vasculitides, granulomatoses, and connective tissue diseases. | 1983 Apr | Problems with the classification and diagnosis of vasculitides are discussed. The evidence related to the pathogenetic importance of immune complexes in vasculitis, both in experimental models and human disease, is reviewed. The finding, by immunofluorescence, of immunoglobulins and complement components in vessel walls has provided indirect evidence of a role of immune complexes in certain forms of human vasculitis. However, specific antigens have been demonstrated in very few instances, notably in some patients with hepatitis B infection. In most forms of human vasculitis there is no information about causative factors. The widely held belief that therapeutic drugs cause an appreciable proportion of cases of human vasculitis appears unfounded. | |
| 6124669 | Serum amyloid-A protein concentration in inflammatory diseases and its relationship to the | 1982 Jul 31 | Serum amyloid-A protein (SAA) is the putative precursor of amyloid-A (AA) protein which forms the fibrils in reactive systemic or secondary amyloidosis. By means of a novel immunoradiometric assay, the concentration of SAA was found to be greatly elevated in patients with rheumatoid arthritis and juvenile chronic arthritis and correlated with activity of their primary disease. However, in patients with systemic lupus erythematosus SAA levels were only modestly raised, even in those with severe active disease, unless significant intercurrent microbial infection was also present. In Crohn's disease SAA levels showed a pattern similar to that seen in rheumatoid arthritis, whereas in ulcerative colitis it resembled that of systemic lupus erythematosus. The level of SAA response in these different disorders corresponds with the incidence of reactive systemic amyloidosis in them. These observations support the view that major increases in SAA levels are a necessary condition for the deposition of this form of amyloid and suggest that prospective monitoring of the SAA concentration in predisposing diseases may help to identify those individuals who are most at risk for amyloidosis. | |
| 6376635 | Use of monoclonal antibodies and F(ab')2 enzyme conjugates in ELISA for IgM, IgA and IgG r | 1984 Jul 6 | Enzyme-linked immunosorbent assays (ELISA) were developed for the measurement of rheumatoid factors (RF) of IgM, IgA and IgG isotypes. Rabbit IgG was used as substrate and the results expressed as units by referral to standards. Rabbit antibody could not be used for detection of IgA or IgG RF without either pepsin-digesting the antibody or inactivating the IgM RF activity of the test sample. When a double antibody system of mouse monoclonal anti-human Fab gamma and goat (Fab')2 anti-mouse IgG was used for the IgG RF assay, pepsin treatment of serum samples was unnecessary. Raised serum IgA RF may be a reliable early marker of erosive rheumatoid arthritis (RA). | |
| 6730729 | [Ultrasound-Doppler analysis of arterial blood flow before and after cryotherapy]. | 1984 Mar | The reduction of oxygen consumption of the synovial membrane and the decrease of juxta-articular muscular tension are amongst others the aims of cryotherapy. The local cold application is followed by a reactive change in blood flow even in distant parts of the body. The knee region of 19 persons was cooled with a cryogel -pack for 15 min and the arterial blood flow of the a. dorsalis pedis was analysed with an ultrasound-Doppler-apparatus. Already in the rest period of 30 minutes before cooling the mean flow decreases and reaches a minimum after 10 min of cryotherapy. Subsequently continuing the therapy, blood flow velocity increases. The parameter independent of the angle is given through the pulsatility index PI (= maximum amplitude/mean flow). Since the mean flow is the denominator of this index the PI-value rises during the first 10 min of cooling and then decreases. The change of flow velocity for the mean value of the group was up to 1.0 cm/sec, i.e. 31% of the initial value. The maximum change of the pulsatility index was about 1.0, i.e. 29% of the initial value. There was no increase of the flow velocity during the test period for a control group of 22 persons with no cryotherapy. The difference was statistically significant (p less than 0.01). The cryotherapy selected includes a 15 minutes application of a cryogel -pack on a cloth, which leads to an average increase in blood pressure in the malleolar region of less than 5%. In spite of cryotherapy in all groups the mean blood pressure in the arm remains constant or decreases slightly because of the position of rest.(ABSTRACT TRUNCATED AT 250 WORDS) | |
| 6336226 | Subclasses of IgG produced in the rheumatoid synovium. | 1984 | The subclass distribution of IgG plasma cells was studied in sections of rheumatoid synovial tissue from 15 cases with seropositive rheumatoid arthritis and 8 cases with various types of seronegative arthritis, using the immunofluorescence technique and monoclonal IgG-subclass-specific antibodies. In most tissues, plasma cells of all the four subclasses were present, and IgG1 was usually the dominating subclass. The frequency of IgG3 plasma cells was usually higher than that of IgG2. The results are partly in contrast with previous observations and do not give evidence for a subclass-specific polyclonal B-cell activator, growth factor or differentiation factor. | |
| 7136590 | Wagner resurfacing hip arthroplasty: preliminary results. | 1982 Oct | Resurfacing arthroplasty according to Wagner was used in 40 hips (31 patients) with coxarthrosis or arthritis. The severe, disabling preoperative pain was eliminated in all patients. There was a considerable increase in range of movement, and the functional results were very good. Of the 24 patients previously in employment but disabled by their hip condition, 22 have returned to their former occupations. Complications occurred in only four cases. | |
| 7167694 | [Scleroderma with progressive facial hemiatrophy and atrophy of the other side of the body | 1982 | The authors report the case of a 28 year old woman suffering from a chronic polyarthritis with a linear sclerodermia and presenting a crossed atrophy involving the left side of the body and the right side of the face and neck. The polyarthritis began at age 6, with signs of systemic illness, resulting early in important joint disability and proceeding with exacerbations and remissions. From the onset of this polyarthritis the patient experienced in the left side of the body highly painful and frequent muscular cramps which became rarer with the occurrence and progression of atrophy on the same side. At age 15, the patient experienced similar cramps in the right face, followed by progressive right hemiatrophy. At age 28, examination showed crossed atrophy involving the left side of the body and the right side of the face, tongue and neck, associated in these territories with several atrophic plaques on the skin, circumscribed alopecia, as well as numerous joint sequella. The laboratory date yielded immunologic abnormalities. On the CT scan the brain was normal but the right facial hemiatrophy involving orbital region and pharynx was visible. On the muscular biopsy there were inflammatory changes in the atrophic territory. Skin biopsies were consistent with sclerodermia. Two clinical points should be emphasized. Firstly, the remarkably crossed topography of the atrophy: the upper limit of the left body atrophy corresponded with the lower limit of the right face and neck atrophy; secondly, the unusual features of the muscular cramps. The relationship between sclerodermic facial hemiatrophy and Parry Romberg syndrome are then discussed: the study of this case and the literature do not provide sufficient criteria to allow description of two separate entities. Finally, the pathogenesis of localized sclerodermia is considered; in this case the association with the immunologic abnormalities and chronic polyarthritis is in favor of the hypothesis of a systemic disorder. | |
| 1153805 | Prostaglandin synthesis by rheumatoid synovium and its stimulation by colchicine. | 1975 Jul | The synthesis of prostaglandins by rheumatoid synovial tissue in organ culture was studied utilizing radioimmunoassay, with antisera to PGB1, PGF1alpha and PGF2alpha. It was established that PGE2 and PGF2alpha were the major prostaglandins formed by analyses of culture media with the two antisera to PGF, before and after alkali treatment. Indomethacin at 5 mug/ml suppressed prostaglandin synthesis, usually to less than 1% of control cultures. Colchicine, 0.1 mug/ml resulted in marked stimulation of prostaglandin synthesis, in some cases over 10 fold. It is suggested, because of the colchicine effect, that the state of the microtubules may regulate the rate of prostaglandin biosynthesis. It is possible that prostaglandin E2 produced by rheumatoid synovia may contribute to the pathogenesis of the inflammatory reaction and lead to destruction of juxta-articular bone in rheumatoid arthritis. | |
| 6605750 | Phenotypic characterization of synovial tissue cells in situ in different types of synovit | 1983 Nov | Immunohistochemical double-staining was performed on frozen sections from synovial biopsies obtained at arthroscopy from 22 patients with different kinds of synovitis. Not only in specimens from patients with rheumatoid arthritis and seronegative chronic polyarthritis, but also in those from patients with synovitis due to psoriasis, trauma, or crystals, were seen many alpha Leu-1-positive T lymphocytes--in most cases predominantly of the alpha Leu-3a-positive T "helper" cell type--in close contact with HLA-DR-positive macrophages/dendritic cells. Presence of many HLA-DR-positive, often OKM1-negative, and irregular sublining cells characterized all forms of chronic arthritis. | |
| 6971132 | Antiglobulins in normal human sera which react with baboon Ig G. | 1980 | Antiglobulins of the Ig M class which react with the Ig G of baboons have been discovered in approximately half the number of normal human sera investigated by the technique of hemagglutination of red blood cells coated with baboon immune serum. The frequency of these antiglobulins is significantly higher among patients suffering from seropositive rheumatoid arthritis but there is no correlation with the presence of Milgröm-type antiglobulins. The inhibition of several sera containing these antiglobulins by the sera of baboon of different species is also reported. | |
| 6615974 | Failure of one design of surface replacement knee arthroplasty due to loosening deformatio | 1983 Jul | Twenty four knee prosthesis femoral and tibial components of the 'load angle inlay' design, removed for loosening and pain were examined in the scanning electron microscope, light microscope, and volume loss measured using a simple gauge. In all cases the deformation of the plastic (femoral) component was seen both by increase in the curvature of the inner surface and wear usually on the edge of the bearing surface. Two pairs were subluxed prior to removal from the patient and these not only produced gross deformation of the plastic components, but maximum volume loss of the components examined; the knees exhibited gross instability. The tibial (metal) components stood up remarkably well in all cases, both in wear and deformation which was minimal but sinking and rotation of the plateau did occur for the most part on the external side. Scratch patterns seen on the tibial components were clearly seen and gave an indication of the direction and amount of sliding between the components; in some cases these scratch lines were in more than one direction probably indicative of loosening and instability. Short deep scratches were usually indicative of bone and/or cement particles embedded in the plastic components, abrasive wear was seen on 92 per cent of the femoral components, and cracks were seen on two-thirds, usually parallel and close to the sides. | |
| 6235068 | Enhanced T helper cell function for the spontaneous production of IgM rheumatoid factor in | 1984 Jun | Co-culture experiments between T and B cells from normal subjects and rheumatoid arthritis (RA) patients were performed in order to assess the immunoregulatory effects of T cells on spontaneous production of IgM rheumatoid factor (IgM-RF). Cultures of normal B cells with normal, autologous or allogeneic T cells failed to synthesize IgM-RF. In contrast, RAT cells promoted IgM-RF production from normal B cells as well as from RA B cells. The 'helper' effect of T cells from RA patients for IgM-RF production was not HLA-DR4 restricted and could not be accounted for by an allogeneic effect. These observations suggest that activated T helper cells may contribute to the production of IgM-RF in RA. | |
| 309474 | A direct binding assay for rheumatoid factor serum antiglobulins using fluorescein-labelle | 1978 Oct | A simple routine method is described for the quantitation of antiglobulin (rheumatoid factor) in human serum irrespective of its immunoglobulin class. Papain Fc fragment of human IgG is labelled with fluorescein isothiocyanate and incubated with the serum. Bound Fc is separated from free Fc by precipitation with polyethylene glycol and measured by the fluorescence of the precipitate. Results are expressed as a percentage of the binding obtained with a normal pool serum. About 60% of seronegative rheumatoid sera and 96% of seropositive rheumatoid sera gave results more than 2SD above the mean value for normal sera. Only 17% of osteoarthritis sera gave positive results by the same criterion. A relationship is also deduced to show that if the test is carried out at a number of Fc concentrations a double reciprocal plot of bound Fc against free Fc enables both the total antiglobulin and the ratio of binding constants of antiglobulin for Fc and for IgG to be calculated. This ratio is approximately unity. Under the standard conditions the results correspond to a measurement of approximately 30% of the total antiglobulins present. | |
| 6252699 | [Antibodies to rubella virus in the blood sera and in the synovial fluid of children with | 1980 Jul | Sera and synovial fluids from children with rheumatoid arthritis (RA) were examined for antibody to some viral and mycoplasmal antigens. Blood sera from the patients were found to have a selective excess of antibody to rubella virus as compared with the age norm. Antibody of this specificity was found in synovial fluids in titers significantly higher than those in the blood. Titers of other antibodies in the synovial fluids were, as a rule, slightly lower than in sera. In the time course of intercurrent respiratory diseases, despite the lack of rubella introduction into the wards, children with RA showed variations in the levels of antibody to rubella virus much more frequently than to other infectious agents. The foregoing data are discussed from the point of view of the etiological association of juvenile RA with rubella virus persistence. The antigen of the latter was found in snyovial membrane cells of 4 children by means of immunofluorescence procedure. | |
| 6980249 | Reduced T lymphoid colony growth in patients with progressive systemic sclerosis and rheum | 1982 Aug | T cell colony growth in semisolid medium, a sensitive indicator of disease-associated lymphoycte defects, was found to be depressed in patients with PSS and RA. In both disorders, more pronounced abnormalities were observed after incubation with suboptimal concentrations of the polyclonal mitogen PHA. Depletion of monocytes by adherence to plastic surfaces of addition of the prostaglandin synthetase inhibitor indomethacin did not correct the growth abnormality observed in PSS. Reduced colony-forming activity could not be attributed to serum inhibitory factors. The cellular abnormalities detected in patients with these connective tissue diseases were less pronounced than those found in SLE patients. | |
| 7373629 | Intraarticular fragmentation of a new Parker-Pearson synovial biopsy needle. | 1980 Mar | An attempt to obtain a synovial biopsy with a previously unused Parker-Pearson needle resulted in intraarticular fragmentation of the tip of the biopsy needle. Since gross inspection of the needle carried out before the procedure did not show any abnormalities, we believe that the manufacturer's material might be defective and that the inner part of this instrument must be made stronger to avoid this complication. |