Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 1101850 | [Utilization of luminescent monospecific sera against human immunoglobulins G, A and M for | 1975 | A method for obtaining luminescent sera, consisting in isolation of pure antigens G, A, M from human gamma-globulin and blood serum from patients with myeloma disease is suggested. A native antiserum was obtained by immunization of rabbits with water-insoluble polycondensate of antigens "sewn" with glutaric aldehyde. Adsorption of antisera as well as specific antigens was carried out with antigen- and antibodyimmunosorbent, the latter being obtained with the help of both glutaric aldehyde and sefarose 4B treated with cyanogen bromide. The sera had a specific titre in the precipitation reaction against their own antigens 1:32 and were highly specific. A globulin fraction was obtained by sedimentation with polyethylene-glycol. Marking of the specific protein with fluoresceine isothiocyanate was carried out using the dialysis method with subsequent purification on sefadex and DEAE-cellulose. The application of the abovementioned sera made it possible to ascertain the character of distribution of deposits of immunoglobulins in glomeruli in systemic lupus erythematodes, glomerulonephritis and in the cells of the synovial fluid sediment in rhematoid arthritis. | |
| 6972984 | Stimulation of human neutrophil chemiluminescence by soluble immune complexes and antibodi | 1981 Aug | Activation of the neutrophil surface membrane may occur with a variety of stimuli, leading to enhanced glucose oxidation via the hexose monophosphate shunt and production of toxic oxygen radicals such as superoxide anion. This metabolic stimulation may be accompanied by light emission or chemiluminescence. The objective of these studies was to determine the effects of soluble and insoluble immune complexes, IgG aggregates, and rabbit anti-neutrophil serum on neutrophil activation as measured by chemiluminescence. Also, the uptake of immune complexes by neutrophils was determined and was correlated with the solubility of the complexes and the levels of induced chemiluminescence. The results indicate that stimulation of neutrophil chemiluminescence and uptake of immune complexes by neutrophils were both closely correlated with the percent precipitation of the immune complexes. Insoluble immune complexes that were avidly ingested by neutrophils also stimulated maximum levels of chemiluminescence. Nevertheless, elevated levels of chemiluminescence were induced by soluble immune complexes that were poorly ingested by neutrophils. Similarly, soluble aggregated IgG induced elevated levels of neutrophil chemiluminescence in a dose-dependent manner. Aggregated IgG induced up to sixfold higher levels of neutrophil chemiluminescence than did equal amounts of monomeric IgG. Finally, rabbit anti-neutrophil serum also caused increased levels of neutrophil chemiluminescence. Since previous studies have shown that sera from some patients with rheumatoid arthritis and Felty's syndrome have increased amounts of neutrophil-binding IgG due to the presence of both immune complexes and neutrophil-reactive antibodies, the effect of these sera on neutrophil chemiluminescence was studied. Positive correlations were observed between the level of neutrophil chemiluminescence induced by the sera and the amount of serum IgG neutrophil-binding activity (r = 0.72, p less than 0.001) as well as the level of immune complexes determined by C1q binding (r = 0.76, p less than or equal to 0.001). Such neutrophil activation by immune complexes or antibodies may reflect production of toxic oxygen radicals and could subsequently affect neutrophil function. | |
| 6994696 | [Immunofluorescence study of reactions between the serum of patients with rheumatoid arthr | 1980 | By means of immunofluorescence using sera against three classes of human immunoglobulins (IgG, IgM, IgA), localization of the components (or one of the components) responsible for reaction of sera from patients with rheumatoid arthritis (RA) with connective tissue antigens was found to be in the cytoplasm of different kinds of cell elements of the connective tissue present in joints. Sera from RA patients collected at different stages of the disease reacted with connective tissue cell elements in titers of 1 : 64 to 1 : 1024, whereas sera from normal subjects (in 33% of specimens) reacted only to 1 : 8--1 : 16. | |
| 6335864 | Cold insoluble globulin: relationship with lymphocytotoxins in autoimmune diseases. | 1984 Oct | Lymphocytotoxic activity is known to better occur at low temperatures and to be relatively enriched in cryoglobulins. As the cold insoluble globulin, Fibronectin, is a main component of cryoglobulins, experiments were carried out to find out whether Fibronectin was involved in the mechanism of cytotoxicity or not. No direct or indirect (complement-mediated) effects were seen. A significant association was observed between lymphocytotoxic activity and circulating immune complexes and between Fibronectin positivity and cytotoxicity in PEG-precipitates of sera from three groups of patients affected with SLE, RA and Ps.A. respectively. As Fibronectin is a cryoprecipitagogue protein, possible implications of these findings are discussed. | |
| 1262375 | Spine fusion for atlanto-axial instability. | 1976 Apr | A follow-up study was done on fifty-seven spine fusions for atlanto-axial instability, classified as Type I - deficient odontoid (twenty-eight patients), Type II - deficient ligaments (twenty-three patients), and Type III - rotatory fixation (six patients). The average age of the patients at the time of the fusion was thirty years, and half had had significant trauma. Symptoms had been present for an average of 12.3 months before fusion. Preoperatively pain was the predominant complaint in twenty-nine; neurological complaints, in twenty-four; and instability in the rest. Of the forty-six Gallie fusions, there was one non-union and of the eleven occipitocervical fusions, two non-unions. Follow-up averaging 4.2 years revealed resolution of symptoms in almost all patients. | |
| 6334939 | The pathogenesis of arthritis in Lyme disease: humoral immune responses and the role of in | 1984 Jul | We studied 78 patients with Lyme disease to determine how immune complexes and autoantibodies are related to the development of chronic Lyme arthritis. Circulating C1q binding material was found in nearly all patients at onset of erythema chronicum migrans, the skin lesion that marks the onset of infection with the causative spirochete. In patients with only subsequent arthritis this material tended to localize to joints where it gradually increased in concentrations with greater duration of joint inflammation. In joints, its concentration correlated positively with the number of synovial fluid polymorphonuclear leukocytes. Despite the prolonged presence of putative immune complexes, rheumatoid factors could not be demonstrated. These observations suggest that phlogistic immune complexes based on spirochete antigens form locally within joints during chronic Lyme arthritis. | |
| 6106345 | Ultrastructural changes of skeletal muscles in polyarteritis nodosa and in arteritis assoi | 1980 | Muscle biopsies from two cases of polyarteritis nodosa (PN) and one of arteritis in assoication with rheumatoid arthritis (RA) were examined by electron microscopy. The histological changes were similar in all three cases. The endothelial cells of the small blood vessels were often hypertrophied. Inflammatory reaction was present mainly in the vicinity of the blood vessels. Individual muscle fibres showed mostly nonspecific degenerative changes. In a case PN, however, annulate lamellae were present in a small number of the muscle fibres. The annulate lamellae have been reported, to our knowledge, in the human skeletal muscles only in a few cases of polymyositis. In addition, two cases, one of PN and of arteritis with RA, showed fine filamentous inclusions in the muscle fibres. Changes were also noted in the motor end-plate. A sural nerve biopsy in a case of arteritis with RA showed changes both in axons and myelin sheaths, in addition to the changes in the blood vessels similar to those in the muscle. | |
| 314005 | Laboratory criteria for a diagnosis of systemic lupus erythematosus. | 1979 Sep 7 | Patients with systemic lupus erythematosus (SLE) and other connective tissue diseases were surveyed for the presence of antibodies to normal DNA, antibodies to a ribonuclease-insensitive acidic nuclear protein, and immune deposits in normal skin. While 80% of patients with SLE had abnormal values for at least two of these three tests, none of the patients with other connective tissue diseases had more than one abnormal value. The presence of RBC autoantibodies was found along with one of the other abnormal laboratory tests in 76% of patients with SLE, including 14% of patients not found to have two abnormal tests. None of the other patients tested had RBC autoantibodies. These findings suggest that a set of laboratory tests can be constructed as criteria for a diagnosis of SLE that would be as specific as the presently employed American Rheumatism Association criteria. | |
| 145832 | Gold-induced immune complex nephritis in seronegative rheumatoid arthritis. | 1977 Dec | Proteinuria, with or without the nephrotic syndrome, developed in 8 patients with seronegative rheumatoid arthritis after the institution of gold therapy. Light microscope examination of renal biopsies showed normal findings in 7, and a focal increase in the mesangial matrix of one glomerulus in the eighth. In all patients immunofluorescence showed deposits of IgG and C3 along the glomerular basement membrane, indicative of immune complex nephritis. The renal biopsies of 5 patients were studied with the electron microscope and subepithelial deposits were detected in all. The Rose-Waaler test for the detection of IgM-rheumatoid factor (IgM-RF) was repeatedly negative in all patients. These results suggest that the development of gold nephropathy may be related to an absence of IgM-RF in serum. | |
| 6897816 | Transient thyrotoxicosis occurred after cessation of steroid therapy in a patient with aut | 1982 Oct | A 51-year-old woman with chronic lymphocytic thyroiditis showed transient thyrotoxicosis at seven weeks after cessation of steroid therapy for treatment of rheumatoid arthritis. Radioactive iodine uptake was only 3% even in the thyrotoxic state. Aspiration biopsy, performed just before the development of hypothyroidism, revealed histological features compatible with chronic lymphocytic thyroiditis. At two months after the onset of thyrotoxicosis, hypothyroidism developed, and she was kept in a euthyroid state by 1-thyroxine replacement therapy. Irreversible hypothyroidism was confirmed by the recurrence of hypothyroidism after cessation of thyroid replacement therapy at one year after the onset of thyrotoxicosis. The causal relationship between the cessation of steroid therapy and the occurrence of transient thyrotoxicosis is discussed. | |
| 3840985 | The histopathologic spectrum in Mycobacterium marinum infection. | 1985 Dec | Review of nine culture-positive cases of Mycobacterium marinum infection revealed a broad range in the histopathologic features of lesions produced by this organism. Four synovial lesions and five cutaneous infections were observed. A range of inflammatory changes were seen in both synovial and skin lesions, varying from mostly acute inflammation with suppuration to a more chronic process with numerous, well-formed granulomas. Organisms were observed in the biopsy sections of only one of the nine cases. Therefore, culture of the biopsy tissue at 30 degrees C is crucial in establishing the diagnosis. These cases emphasize the importance of considering mycobacterial infection and performing cultures even when granulomatous changes in the synovium or skin are subtle. | |
| 868500 | Operative treatment of ulnar nerve neuropathy in the elbow region. A clinical and electrop | 1977 | Thirty-three patients with ulnar nerve neuropathy due to a lesion in the ulnar groove were operated upon during a 5-year period. All the patients were analyzed preoperatively. One-third of the patients had a history of alcohol abuse. Twenty-five of the patients were subjected to anterior transposition of the ulnar nerve. The results after transposition have been compiled according to etiology, duration, age and alcohol abuse. All seven patients with only subjective symptoms improved; 11 out of 18 (61 per cent) with motor and/or sensory loss also improved but only six (33 per cent) recovered completely. Overall, 21 patients (84 per cent) were improved by the operation. In 11 of the transposition cases the ulnar nerve was examined electrophysiologically before and after operation and an improvement of the motor conduction velocity within the elbow segment of the nerve was found in 10 cases. | |
| 785344 | Laboratoire d'Hygi'ene de la Facult'e de M'edecine de Lyon Universit'e Claude-Bernard, Dom | 1976 Jun | The objective of this study was to investigate the false-positive reactions in the Kline, FTA200 and FTA-ABS tests in acute disseminated lupus erythematosus, polyarthritis and controls. Though the false-positive Kline tests are frequent in autoimmune diseases, false-positive FTA200 when made on non lyophilised treponema are uncommon. On the other hand, when lyophilised treponema are used as substrate for the immunofluorescence, false-positive FTA200 and FTA-ABS are more common in the patients with autoimmune diseases than in controls. The false-positives had two patterns of fluorescence "beaded" or "homogeneous". The present investigation suggests that the false-positive reactions of the beaded type are due to the presence of anti-DNA antibodies in the sera. | |
| 7086091 | Total joint replacement at the base of the thumb--preliminary report. | 1982 May | Twenty-nine patients have been studied after trapeziometacarpal total joint replacement with a system of the author's design using a titanium metacarpal component and a polyethylene trapezium component cemented to bone with polymethyl methacrylate. Replacement was performed for arthritis, failure of attempted arthrodesis, previous Silastic or total joint arthroplasty failure, or postparalytic fibrous ankylosis of the joint. Twenty-six patients have achieved a good range of painless motion since the study was initiated in 1974. There have been no cases of implant fracture or infection, but three cases have demonstrated loosening at the cement--bone interface. The results appear to warrant cautious optimism at this time. | |
| 371338 | Renal transplantation in amyloidosis. | 1979 | During a three-year period renal transplantation was performed in 12 patients with amyloidosis. This disease was primary (or the cause unknown) in two cases and secondary in ten. In the latter cases the primary disease was rheumatoid arthritis in six, ankylosing spondylitis in one, osteomyelitis in two and tuberculosis in one. Five of the 12 patients were alive one year after transplantation. Two years after transplantation four out of seven were alive. Graft survival was the same. At the end of the three-year period five patients were alive. In two of these cases renal biopsy showed amyloid deposits in the transplant two and three years, respectively, after the transplantation. | |
| 6147499 | Anti-DNA antibody idiotypes in systemic lupus erythematosus. | 1984 Aug 25 | Monoclonal anti-DNA antibodies prepared by the hybridoma technique were used for an analysis of idiotypes of anti-DNA antibodies in systemic lupus erythematosus (SLE). Serum levels of one idiotypic marker, 16/6/R, were higher than normal in 40 of 74 patients (54%) with active SLE, compared with only 6 of 24 patients (25%) with inactive SLE, 9 of 38 patients (25%) with rheumatoid arthritis, and 4 of 96 normal subjects (4%). Levels of the 16/6/R idiotypic marker were determined in serially collected serum samples from 12 patients with SLE. Concordance was found between idiotype levels and clinical activity in 8 of the 12 patients. Levels of the 16/6/R idiotype tended to correlate with levels of antibodies to double-stranded DNA, but in some cases the clinical status was reflected better by the idiotype levels than by the levels of anti-double-stranded-DNA antibodies. Measurement of idiotypes of anti-DNA antibodies may provide information valuable in monitoring the clinical course of patients with SLE. | |
| 6384036 | Cytokine modulation of chondrocyte metabolism--in vivo and in vitro effects of piroxicam. | 1984 Jun | Changes in the macromolecular proteolycan (PG) and collagen of the cartilage matrix may culminate in irreparable tissue destruction. Molecular modifications appear to result from: (A) exogenous proteinases, (B) endogenous chondrocyte proteinases whose synthesis and release is modulated by exogenous non-enzymatic cytokines (CKs) and (C) quantitative and/or qualitative alterations in chondrocyte PG and collagen synthesis which are potentially induced by exogenous CKs. Studies have recently been initiated to determine the effect of piroxicam on the synthesis and activity of such metabolic regulatory CKs in patients with rheumatoid arthritis and osteoarthritis, and in age-, sex-, and race-matched controls. Therapeutic doses of piroxicam alone had no effect on the anabolic or catabolic function of chondrocytes. Current studies concern the effect of piroxicam on: (a) spontaneous and lectin-driven production by peripheral blood monocytes and T-cells of trypsin-sensitive, heat-labile CKs (interleukin 1, lymphokine) which, in a protein- and RNA-synthesis-dependent manner, induce a concentration and duration of substrate exposure dependent release of chondrocyte PG- and collagen- degrading neutral proteinases in cartilage organ and chondrocyte suspension culture systems; (b) spontaneous and lectin-driven synthesis by peripheral blood T-cells of lymphokines capable of suppressing chondrocyte PG, glycosaminoglycan, protein, collagen and nucleic acid synthesis in a quantitatively reversible manner; (c) pathological synovial membrane synthesis of such catabolic-inducing and anabolic-modulatory CKs. These experimental model system are reviewed together with preliminary data on the effect of piroxicam. | |
| 6091238 | Analogues in immunology. | 1984 Apr | A number of immunological functions has been found to be endowed with biological rhythmicity. Variations of peripheral blood lymphocytes, immediate and delayed hypersensitivity responses, and reactivity of the immune system to antigenic challenge have consistently shown circadian bioperiodicity. By employing a panel of monoclonal antibodies specific for the various lymphocyte subsets, we have been able to detect rhythmic variations in the number of total T lymphocytes, as well as of the T helper (TH) and the T suppressor/cytotoxic (Ts) subsets. Available evidence clearly indicates such circadian variations to be due to compartmentalization of circulating lymphocytes in several lymphoid organs. Biological rhythms (circadian, circaseptan, etc.) have been demonstrated in several immunological situations of clinical interest, including variations of TH and Ts cells in patients with rheumatoid arthritis incidence of allograft rejections, antitumoral effectiveness of chemoimmunotherapy, and allergic symptoms. In addition, the recent availability of synthetic analogues of some thymic peptide hormones and of the synthetic ACTH 1-17 (Synchrodyn 1-17 is greatly contributing to the elucidation of the role of such hormones in the immune regulation process. A preliminary account is reported of the experiments performed with the aim of raising conventional rabbit antisera to the peptide analogue ACTH 1-17, as well as of the consistently negative results which were obtained when 578 human serum samples (from patients treated with such synthetic peptide) were screened by a radioimmunoassay for the occurrence of anti-ACTH 1-17 antibodies. It is emphasized that the recognition of the rhythmic variations of several immunological mechanisms and of the crucial role played by the analogues of adrenal corticotropic and thymic hormones in immune regulation has favoured significant advances in the fast growing area of chronoimmunology. | |
| 393464 | A comparative study of fenbufen and indomethacin in patients with rheumatoid arthritis. | 1979 | A short-term, double-blind crossover study was completed in 29 patients with definite rheumatoid arthritis to compare the antirheumatic activity of indomethacin (100 mg/day) with that of fenbufen (800 mg/day). The patients were randomly allocated to two groups and treated for a period of 6 weeks with each of the drugs. Subjective and objective assessment of rheumatic activity showed that indomethacin produced significant improvement only in morning stiffness and walking time; however, following fenbufen a significant improvement in walking time, grip strength and joint swelling resulted. Both the observed and the patients assessed the fenbufen period as significantly better than the baseline and that following indomethacin. | |
| 2998407 | Antiarthritic drugs containing thiol groups scavenge hypochlorite and inhibit its formatio | 1985 Nov | We investigated the effect of antiarthritic drugs containing thiol groups, such as D-penicillamine, tiopronin (N-[2-mercaptopropionyl]glycine), sodium aurothiomalate, and aurothioglucose, on the chlorinating activity of myeloperoxidase purified from human leukocytes. Hypochlorite, the reactive product of the reaction catalyzed by myeloperoxidase, was effectively scavenged by these antiarthritic drugs, and in addition, D-penicillamine and tiopronin inhibited myeloperoxidase itself. The above-mentioned effects of these drugs were observed at concentrations that occur in the serum of rheumatoid arthritis patients treated with these agents. We suggest that the therapeutic effect of these antiarthritic drugs may be due to the protection of tissues against the reactive HOCI released by activated granulocytes at inflamed sites. |