Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 7065112 | Demonstration of AA-protein in formalin-fixed, paraffin-embedded tissues. | 1982 Feb | AA-protein was identified by SDS-acrylamide electrophoresis in amyloid fibrils fixed in formalin after isolation from fresh-frozen tissues obtained from patients with familial Mediterranean fever (FMF) amyloidosis and idiopathic AA-amyloidosis and, following deparaffination, rehydration and homogenization of embedded formalin-fixed tissues of old autopsy cases of the hereditary amyloidosis of FMF and amyloidosis acquired in association with tuberculosis, bronchiectasis, and rheumatoid arthritis. That AA-protein is unaltered by formalin was firmly established by agar gel diffusion using specific rabbit anti-AA serum. By contrast, AL proteins could not be demonstrated either in formalin-fixed amyloid fibrils derived from fresh-frozen tissues of a patient with presumably AL-amyloidosis dominated by cardiomegaly and one with AL-kappa amyloidosis or in blocks of cases of familial neuropathic amyloidosis, multiple myeloma, and idiopathic amyloidosis with cardiopathy. AA-protein is not denatured by formalin and retains its typical electrophoretic, chromatographic, and immunologic characteristics even 30 years after fixation and paraffin-embedding. | |
| 6211763 | The drug treatment of osteoarthritis. | 1982 | Recent recognition of the importance of inflammation and the efficacy of anti-inflammatory drugs in osteoarthritis has increased their importance in the routine management of the disease. Anti-inflammatory drugs do more than just relieving pain; they reduce the duration of morning stiffness, stiffness after sitting and the number of tender joints. Patients usually prefer them to simple analgesics. The choice of anti-inflammatory drugs is determined largely by individual variation in response so that it may be necessary to try a number of different compounds before finding one which suits a particular patient. Intra-articular steroids are disappointing in that though effective, their action is very brief. Intra-articular orgotein may have a useful role in the treatment of osteoarthritis. Simple analgesics are useful for patients with mild or intermittent pain when regular treatment is inappropriate. Specific therapy, like penicillamine for rheumatoid arthritis or allopurinol for gout, is urgently required. Better understanding of the pathogenesis of the disease may make this possible. | |
| 7247193 | Subconjunctival lymphoma: a review of six suspected cases and the use of immunologic surfa | 1981 Apr | Six cases of subconjunctival tumor clinically compatible with the appearance of subconjunctival lymphoma were evaluated. Two of the patients, Cases 1 and 2, were under treatment with either prednisone or gold therapy for severe rheumatoid arthritis. Patient 3 manifested further evidence of systemic disease (auxillary lymph node). Of the remaining cases, patient 4 had no further evidence of systemic involvement, whereas patient 5 was suspected of having a gastrointestinal tumor. The last case, a patient known to have pulmonary lymphoma, presented with dry eye and multiple subconjunctival lymphomatous masses. Immune surface marker studies demonstrated a B-cell monoclonal subconjunctival lymphoma, suggestive of an improved survival when compared to null cell-type lesions. Immune surface marker studies offer a new commercially available method for classifying and prognosticating subconjunctival lymphomas. | |
| 6780020 | Clinical usefulness of urinary 3-methylhistidine excretion in indicating muscle protein br | 1981 Jan 31 | Urinary excretion of the post-translationally modified amino-acid 3-methylhistidine, derived from the contractile proteins actin and myosin, was measured in patients with conditions associated with nitrogen loss. The ratio of 3-methylhistidine:creatinine excretion, a measure of the fractional catabolic rate of myofibrillar protein was increased in severe injury, thyrotoxicosis, neoplastic disease, prednisolone administration, and sometimes Duchenne muscular dystrophy. In myxoedema, osteomalacia, and hypothermia the ratio was decreased; and starvation, elective operations, and rheumatoid arthritis had little effect. Provided that the diet is meat free, measurement of urinary 3-methylhistidine may provide useful information on the cause of protein loss. | |
| 7246618 | Cohen syndrome: further delineation and inheritance. | 1981 | Four sibs, 2 males and 2 females, were found to have the Cohen syndrome. All had moderate mental retardation, microcephaly, hypotonia, and narrow hands and feet with elongated fingers and toes; 3 were short of stature (2.0-3.5 SD below the mean) with weight between 10th and 50th centile and truncal obesity. Most of the facial characteristics of the syndrome were present: exotropia, prominent ears, short philtrum, and high nasal bridge. Each manifestation varied in severity from one sib to the other. The younger girl also had rheumatoid arthritis. Mild delay of puberty was described in 3 of the sibs. However, one of them has delivered a male infant with normal appearance whose psychomotor development has been normal (as of 9 months). No endocrine problems were documented in the sibship. All patients had normal chromosomes. The data on this sibship support the hypothesis of autosomal recessive inheritance of the Cohen syndrome. Microcephaly and short stature should be stressed as frequent manifestations of the syndrome. The variable expressivity, even among sibs, may be responsible for the paucity of reports on the mildest forms of the Cohen syndrome. | |
| 6971085 | [New aspects of the theory inflammation (immune inflammation)]. | 1981 | Immune inflammation (II) arises on the basis of immunopathological mechanisms and acquires certain qualitative and quantitative features. It develops in a sensitized host following a specific immune response. Several kinds of II are to be distinguished differing from each other by the prevalence of disorders in cellular or humoral immunity, the pattern of the antigen, involvement in the response of immunoglobulins of different classes, complement components and a set of mediators. According to Sell's scheme, six immunopathological mechanisms are distinguished which underlie the development of early (like immediate type hypersensitivity) or late (like delayed type hypersensitivity) immune inflammation. Sometimes humoral and cellular mechanisms alternate in II which is reflected in the morphological picture of inflammation. This is illustrated by an example of the study of tissue inflammatory reactions in the synovial membrane in rheumatoid arthritis. Immune inflammation shows a trend to self-reproduction and continuous course with periods of remission and exacerbation of different durations. Timely recognition of immune inflammation is important both theoretically and practically. | |
| 7371240 | Surgical implications of biomechanics of the foot and ankle. | 1980 Jan | Because of the nature of the alignment the joints of the lower extremity, any significant loss of alignment or function will place increased stress on the remaining joints. An ankle arthrodesis must be carefully aligned into slight valgus and neutral dorsiflexion, plantar flexion and the same degree of external rotation as the opposite leg. A subtalar arthrodesis should be aligned in 5 degrees of valgus. The forefoot should be placed in a plantigrade position when arthrodesis of the subtalar or transverse tarsal joint is contemplated. The stability of the longitudinal arch and forefoot is dependent in part upon the integrity of the metatarsophalangeal joints and as such, neither a resection of the metatarsal head or base of the phalanx should be carried out except under extreme disease states, such as rheumatoid arthritis. If an arthrodesis of the first metatarsophalangeal joint is contemplated, it is important that the joint be aligned in approximately 5 degrees to 10 degrees of valgus and 15 degrees to 25 degrees of dorsiflexion, depending upon the heel height that the patient anticipates using. When considering a tendon transfer about the foot and ankle, the relationship of the tendon to the axes of the subtalar and ankle joint requires special consideration. | |
| 399898 | Human leucocyte elastase and cathepsin G: structural and functional characteristics. | 1979 | Two of the major enzymes present in an released from neutrophil granulocytes are the endoproteinases elastase and cathepsin G. While the former is believed to be one of the major causative agents responsible for tissue destruction in emphysema and rheumatoid arthritis, little is known about the function of cathepsin G. We have recently developed simple procedures for isolating the isoenzymes of each type of proteinase as well as for their specific controlling plasma inhibitors. We have also prepared synthetic substrates and inhibitor analogues. Some sequence studies have been initiated and the results indicate homology of these enzymes not only with each other and with the pancreatic proteinases but also between cathepsin G and proteolytic enzymes present in muscle and mast cell tissue. Significantly, both types of enzyme can degrade the structural protein myosin, as well as elastin and proteoglycan. However, their relative importance in muscle protein turnover or muscle disease has not yet been clarified. | |
| 56040 | [Lupus syndrome during treatment with isoniazid]. | 1975 Oct 23 | In a young girl, aged 17 years, there occurred, 9 months after starting treatment with isoniazid and rifampicin, clinical signs of rheumatoid arthritis with facial erythema of vespertilio type. The presence of L.E. cells and antinuclear antibodies with a high titer, the rapid disappearance of all the clinical signs on stopping isoniazid, and the transient exacerbation of the syndrome on giving a single dose of isoniazid, gave the diagnosis of systemic lupus erythematosus induced by isoniazid. 18 months after stopping the drug, the patient is apparently cured, but there persists in the serum antinuclear antibodies in low concentration (1/200). A study of acetylation of isoniazid in this patient and her 3 sisters, showed that they were all of the slow acetylating phenotype. The late prognosis of this disease and the physiopathological value of the slow acetyl phenotype are worth discussion. | |
| 4093919 | Fibroblast growth factors in scleroderma. | 1985 Dec | Sera from patients with progressive systemic sclerosis (PSS), compared to normal sera, contained increased fibroblast growth promoting factor(s) (FGPF) for autologous and heterologous PSS skin fibroblasts and fibroblasts from normal donors. The relative stimulatory effect of FGPF was unaltered by dilution, mixing with normal serum, or media refeeding. The mean value of 42 PSS sera tested showed growth promoting activity significantly greater than the mean level of activity obtained with 42 sex matched normals (p less than 0.001). FGPF in sera from 25 patients with rheumatoid arthritis did not differ from normal controls. No correlation was seen between growth enhancement and patient age or medications. FGPF was present in serum plasma and is unlikely from platelets because serotonin and purified platelet factor produced no equivalent growth stimulation. FGPF was nondialysable, heat labile and insensitive to ribonuclease A. Disulphide bond integrity was required since 20 mM dithiothreitol destroyed activity. FGPF was excluded by an XM-50 filter after Sephadex G100 gel filtration. | |
| 4030250 | Ocular inflammation in autoimmune MRL/Mp mice. | 1985 Sep | Congenic mice of the MRL/Mp strain spontaneously develop an autoimmune connective tissue disease that shares immunologic and histopathologic features with the human disorders systemic lupus erythematosus, rheumatoid arthritis, and systemic vasculitis. The autoimmune disorder in these mice is markedly accelerated by the recessive gene lpr. Older MRL/Mp-lpr/lpr mice develop significant inflammatory ocular disease, including choroiditis, scleritis, and orbital vasculitis. Animals of both the MRL/Mp-+/+ and MRL/Mp-lpr/lpr substrains develop lacrimal gland inflammatory infiltrates. The MRL/Mp mouse provides a potential model for ocular inflammatory disease and for Sjögren's syndrome. | |
| 3897532 | Nuclear matrix antibodies in rheumatic diseases. | 1985 Apr | We describe a new type of human antinuclear antibody (ANA) reacting with the nuclear matrix by indirect immunofluorescence technique. Cultured cells of human and animal origin were used as substrate. Before assay, cells were extracted with buffers to remove other components of the nuclei leaving the matrix intact. Two types of immunofluorescence staining patterns were seen: homogenous and speckled. When human embryonic fibroblasts were used as targets, sera from patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) contained significantly more nuclear matrix antibodies of the homogenous type than the control group. These antibodies were often, especially in SLE, species cross-reacting. (When PTK-2 cells were used as substrate the difference between SLE and RA was also significant [60 vs 8%]). Antibodies with speckled staining pattern were seen only in the patient sera. Their highest incidence (35%) was found in sera from patients with the primary sicca syndrome. The nuclear matrix may be a major target for ANA in rheumatic diseases. | |
| 3982369 | Urinary bladder tumors following cyclophosphamide therapy: a report of two cases with a re | 1985 | Sterile hemorrhagic cystitis following cyclophosphamide (Cph) therapy is a relatively frequent and well-documented phenomenon. On the other hand, cancer of the urinary bladder associated with Cph therapy is rarely observed. We present two cases and summarize 32 patients reported in the literature. Thirty patients had malignant disease elsewhere, 27 of them nonsolid tumors. The other four patients received treatment with Cph for nonmalignant disease (systemic lupus erythematosus--3; rheumatoid arthritis--1). The bladder tumor developed several months to years after treatment. In 20 patients hemorrhagic cystitis antedated the tumors. We conclude that hemorrhagic cystitis in patients on Cph therapy should indicate at least temporary withdrawal of the drug. The decision to resume treatment should be taken with extreme caution considering the risk of the development of bladder cancer. | |
| 6547388 | Inflammation-mediated osteopenia in the rat: a new animal model for pathological loss of b | 1984 Jul | We have developed a rat model of inflammation-mediated osteopenia. Generalized loss of trabecular bone occurs in the rat after sc injection of nonspecific irritants such as talcum (magnesium silicate) and cotton wool (Cellulose). Although it appears likely that a systemic mediator of bone resorption is responsible for these effects, the loss of bone was not due to increased PTH secretion, since it occurred in parathyroidectomized rats, nor due to excessive 1,25-dihydroxyvitamin-D3 production. In parathyroidectomized rats, this inflammation was associated with significant increase in serum calcium within 4-7 days independent of its cause. Identification and characterization of this mechanism may provide insight into the bone loss associated with chronic inflammatory diseases such as rheumatoid arthritis and periodontal disease. | |
| 6606278 | [Enzyme breakdown of immune complexes]. | 1983 Sep | 4 types of immune complexes according to the Heidelberger curve were prepared as an in vitro model for immune complexes in rheumatoid arthritis and collagen diseases. Immune complexes of high and moderate antigen excess and immune complexes of high and moderate antibody excess were incubated with increasing concentrations of a mixture of enzymes or papain or pancreatin. The concentration of immune complexes was determined by a solid phase C1q radioimmunoassay before and after incubation. Up to 90% of the complexes of antigen excess were disintegrated even by low doses of enzymes (5-10 mg%); total cleavage appeared at 80 mg% enzyme concentration. Complexes of the antibody excess zone were gradually disintegrated by enzyme concentrations gradually increasing from 5 to 80 mg%, where total cleavage appeared. Single enzymes showed less cleavage activity than enzyme mixtures. Although enzymes were administered to supernatants of the Heidelberger precipitation containing soluble immune complexes as well as enzyme inhibitors, enzymatic activity was not impaired and immune complexes were disintegrated. The results of these investigations are discussed in regard to treatment with enzymes. | |
| 6342551 | Epidermal nuclear immunoglobulin deposits in some connective tissue diseases: correlation | 1983 Apr | In-vivo nuclear deposits of IgG were demonstrated by direct immunofluorescence in epidermal cells of normal skin from 6 patients with serum antibodies to an RNase-sensitive extractable nuclear antigen (ENA). Addition of complement to the skin sections showed that C3 could bind to epidermal cells with IgG deposits. A skin biopsy from a patient with polymyositis and serum antibodies to ENA, but without nuclear IgG deposits, showed nuclear binding of C3 after addition of complement to the skin sections. The clinical diagnoses of patients with immunofluorescent staining of epidermal cells were mixed connective tissue disease (MCTD) 4 cases, systemic lupus erythematosus (SLE) 2 cases, and polymyositis 1 case. No epidermal nuclear IgG deposits could be demonstrated in 5 cases of SLE, 2 cases of MCTD, one case of polymyositis, or 15 cases of rheumatoid arthritis without antibodies to ENA. | |
| 6291808 | An improved radioreceptor assay for TSH receptor antibodies. | 1982 Oct | An improved receptor assay for TSH receptor antibodies is described in which detergent-solubilized TSH receptors and 125I-labelled TSH are used. Normal human serum and immunoglobulin concentrates from normal serum showed little effect on the interaction between labelled TSH and detergent-solubilized receptors whereas immunoglobulin concentrates from some Graves sera caused marked inhibition of TSH binding. Precipitation with polyethylene glycol was the most convenient way of preparing immunoglobulin concentrates and using this technique the assay could be completed in a few hours. The coefficient of inter assay variation at 51% inhibition of labelled TSH binding was 3.7% and analysis of serum samples from patients with Graves' disease (n = 42), Hashimoto's disease (n = 26), multinodular goitre (n = 9), rheumatoid arthritis (n = 10) and normal donors (n = 35) suggested that the assay could detect TSH receptor antibodies in about 80% of patients (treated and untreated) with Graves' disease. | |
| 7093001 | [Serological types of Yersinia enterocolitica isolated from humans and from environment]. | 1982 Feb | A total of 1061 Y. enterocolitica strains isolated from patients with different clinical manifestations of yersiniosis (acute intestinal disease, hepatitis, generalized infection, meningitis, rheumatoid arthritis, chronic intestinal disease), from foodstuffs, from animals and from open water reservoirs have been studied. This study has revealed that the strains of serovars 09 and 03 are mainly isolated from humans (66% and 30.5% of cases, respectively). The strains of serovar 05B occur in 3%, and serovar 08 in 0.5% of cases. The clinical manifestations of infection have not been found to depend on the serovar of the strain causing this infection. All Yersinia serovars produced infections in children and adults with equal frequency. Y. enterocolitica strains belonging mainly to serovars 09, 03 and, less commonly, 05B and 08 circulate in different areas of our country. The same serovars of Yersinia have been isolated from foodstuffs (milk, meat, vegetables) and washings obtained from various objects of the environment, which indicates their role as the factors of infection transfer. | |
| 7099337 | Plasma exchange in the successful treatment of drug-induced renal disease. | 1982 | A 20-year-old girl with rheumatoid arthritis who has been treated with D-penicillamine for 7 months presented with fulminating Goodpasture's syndrome and a focal necrotising glomerulonephritis. A 35-year-old man who had been taking a low dose of phenytoin for epilepsy presented with nephrotic syndrome and a mesangiocapillary glomerulonephritis. CH50, C3 and C4 were low and a high level of immune complexes was detected. Both patients had severe lesions which were unlikely to recover on immunosuppression alone so were treated with intensive plasma exchange. 2 patients are described with serious drug-induced renal disease who recovered with intensive plasma exchange and immunosuppression. | |
| 289923 | Condylysis in a patient with a mixed collagen vascular disease. | 1979 Sep | Condylysis has not previously been described as a complication of a mixed collagen vascular disease. A case is presented in which apertognathia and mandibular retrognathism occurred secondary to condylysis in a 26-year-old woman with features of rheumatoid arthritis, systemic lupus erythematosus, scleroderma, and Sjögren's syndrome. The disease was manifested by polyarthritis, morning stiffnes, subcutaneous nodules, and acrosclerosis. Important laboratory findings included RA slide latex negative, increased DNA binding, ANA positive, ENA negative, and an abnormal parotid scan. The malocclusion secondary to condylysis was corrected by surgical procedures usually employed for the treatment of mandibular retrognathism and apertognathia on a developmental basis. |