Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 3878240 | Serum amyloid P component and autoimmune parameters in the assessment of arthritis activit | 1985 Sep | The concentration of immunoglobulins, anti-ssDNA, anti-dsDNA, anti-TNP antibodies, IgM rheumatoid factor, C3, immune complexes and serum amyloid P component (SAP), in the serum were measured in 68 male and female MRL/lpr/lpr mice, a strain affected with a systemic autoimmune disease. The degree of lymphoproliferation was assessed by the spleen weight. Spontaneous secretion of immunoglobulins and anti dsDNA antibodies were measured in spleen cell cultures. All mice presented age related increases or decreases (C3) in the level of measured parameters. Inflammatory lesions were detected, by light microscopy in the joints of all mice. There was a significant correlation, in both sexes between the serum level of SAP and the severity of the polyarthritis, as assessed by light microscopy. In female mice the levels of anti-dsDNA antibodies, immunoglobulins, either measured in serum or in the spleen compartment, and circulating immune complexes also showed correlation with the activity of the arthritis, but neither of these variables correlated as closely with arthritis scores as did serum SAP. | |
| 300311 | Enhancement of in vitro immunoglobulin synthesis of human lymphocytes by lysosomal enzymes | 1977 Feb | The effect of human peripheral blood polymorphonuclear leucocyte (PMN) extracts and PMN granule lysates on in vitro immunoglobulin (Ig) synthesis by autologous peripheral blood mononuclear cells was studied. The mononuclear cells were cultured for 3 days with or without autologous plasma. Newly synthesized Ig in the culture supernatants was measured using 14C-labelled amino acids by an immune coprecipitation method. Upon addition of a PMN extract to plasma-free cultures Ig synthesis was stimulated, the mean stimulation index (SI) of cultures from thirteen individuals, including nine normals, three patients with rheumatoid arthritis and one with psoriatic arthritis being 1-8 +/- 0-2 in comparison with control cultures (P less than 0-05). By contrast, in 10% fresh autologous plasma, PMN extracts yielded a mean SI of 0-9 +/- 0-1 indicating inactivation of the active extracts by plasma inhibitors. In experiments using PMN granule lysates containing high concentrations of beta-glucuronidase and cultured in RPMI 1640, the mean stimulation index was 3-2 +/- 0-7. Stimulation of Ig synthesis was also produced by trypsin. Stimulation of Ig synthesis was also produced by trypsin. Stimulating factors in PMN extracts were inhibited by Trasylol, a protease inhibitor. These results indicate that trypsin and proteolytic lysosomal enzymes in PMN increase Ig synthesis of human peripheral blood mononuclear cells. They suggest a possible new role of PMN in the potentiation of immunoglobulin synthesis. | |
| 970232 | A five-year survey of human Yersinia enterocolitica infections in Hungary. | 1976 | Human infections associated with Yersinia enterocolitica in Hungary in the years 1969-1974 have been surveyed. During this period the public health laboratory network isolated 1355 strains from 1096 persons. The number of isolates according to sero-groups was: 1343 O3, 6 O9, 2 O1, 2a, 3 and 1 strain for O5, O6, O1O and O15 each. (ii) A total of 2192 serum specimens from patients gave positive agglutination reaction with antigen O3 in 26.8%, with antigen O9 in 3.2%. (iii) Fifty-six per cent of bacteriologically positive persons had enteritis. Other clinical forms (pseudoappendicitis and other abdominal complaints, erythema nodosum, rheumatoid arthritis) were encountered in O 1-2.7%. Symptomless excreters of Y. enterocolitica amounted to 23.1% of all positive persons. (iv) Patients with enteritis and symptomless excreters were rather evenly distributed between 10 and 60 years of age; 1--9-year-old children were affected more frequently (47.7% of all positive persons). Six distribution was, males: females = 1.5 : 1. In seasonal incidence yersiniosis differed from other enteric diseases: it showed a peak in the autumn-winter months. Sporadic cases and family outbreaks were the most frequent; epidemic infections in nurseries were also recorded. (v) Out of 59 animal strains 39 group O3 cultures were isolated from pigs, which may be assumed as the most important reservoir of yersinosis in Hungary. | |
| 6604529 | The effects of IgM rheumatoid factor on EAM and EAG rosette formation with Fc receptor-bea | 1983 Sep | IgM rheumatoid factors (IgM-RF) and IgM from normal donors (IgM-C) were tested for their effects on rosette formation of Fc receptor (FcR)-bearing lymphoid cells with IgM- or IgG-coated bovine red blood cells (EAM or EAG). All cell populations tested bearing FcR for IgG (FcRG) showed reduced numbers of EAG rosettes formed in the presence of IgM-RF; IgM-C had no effect on EAG rosette formation with FcRG-bearing cells. T cells or L cells bearing FcRM showed reduced numbers of EAM rosettes in the presence of any IgM preparation. In addition, IgM-RF facilitated EAG-rosette formation with TM cells. These studies confirm that native IgM can bind to FcRM, blocking EAM rosettes, and that IgM-RF can modulate the binding of an IgG-containing immune complex to FcR-bearing cells. | |
| 6603311 | The prevalence of antibodies to histones induced by procainamide in old people, in cancer | 1983 Apr | Antibodies to histones were found to be most commonly responsible for the positive antinuclear antibody (ANA) test in asymptomatic patients treated with procainamide, in old people, in patients with neoplastic diseases, and in young women affected with a rheumatoid-like disease. Only in a very few patients were antibodies to dDNA and nucleoproteins demonstrated. Antibodies to nDNA were not found. The antibodies to histones were demonstrated by two methods: absorption of ANA-positive sera with a histone solution and subsequent performance of an ANA test; and acid elution of histones from thyroid sections followed by histone reconstitution. | |
| 493891 | Joint involvement in systemic sclerosis. | 1979 | Eleven out of 24 patients with systemic sclerosis had radiological features of inflammatory polyarthritis. Juxta-articular osteoporosis was present in 9 patients, erosions in 5 and loss of joint space in 6 patients. Only one patient had a positive rheumatoid factor. Titres of rheumatoid factor, anti-nuclear factor, and plasma viscosity were comparable in patients with and without radiological abnormalities. Measurements of grip strength and finger-palm flexion also were similar in the two groups. However, in individual cases joint or tendon sheath disease was associated with loss of function. | |
| 4093917 | Retinal vasculitis in mixed connective tissue disease. A fluoroangiographic study. | 1985 Dec | The eyes of 20 consecutive patients with mixed connective tissue disease (MCTD) were studied by ophthalmologic and retinal fluoroangiographic examinations (RFA) and compared with the findings in 18 consecutive patients with primary Sjögren's syndrome (SS) and 50 with systemic lupus erythematosus (SLE). Six of the 20 MCTD patients had abnormal RFA with thickening of capillary walls that stained and progressively leaked fluorescein. None of these lesions could be seen in ophthalmoscopy, where only one patient had cotton wool spots that in RFA were found to correspond to focal areas of ischemia, probably resulting from capillary lesions. Only one patient with primary SS showed capillary leakage, whereas 13 patients with SLE had RFA abnormalities that included microaneurysms in 9, capillary lesions in 6 and drusen in one. No microaneurysms were found in MCTD patients. The differences between MCTD, SLE and SS patients may reflect differences in the quality, quantity, frequency and chronicity of immune complex formation and deposition in these 3 diseases. | |
| 6718953 | Immunoglobulin deposits in the epidermis of patients with primary Sjögren's syndrome. A c | 1984 | In biopsies from macroscopically healthy skin, IgG deposits were demonstrated in the intercellular areas of the epidermis by a direct immunofluorescence technique, in 17 of 25 consecutive patients with primary Sjögren's syndrome. Intercellular epidermal deposits of IgA were found in 4 and of IgM in 1 of the 25 patients. Deposits of IgG in the dermal blood vessel walls were found in 9 patients, IgA in 1 and IgM in 3 patients. In 3 of 23 patients with secondary Sjögren's syndrome intercellular IgG deposits were found in the epidermis, and in 2 of these 3 patients IgA deposits were likewise demonstrated. No immunoglobulin deposits were found in the dermo-epidermal junction zone in patients with primary and secondary Sjögren's syndrome. No deposits of immunoglobulin were found intercellularly in epidermis or in dermal blood vessel walls in 23 sex and age matched healthy controls. Blood samples obtained simultaneously from all patients and controls showed no correlation of serum IgG levels with deposits in the skin. We conclude that investigation for IgG deposits in the intercellular area of the epidermis and/or in the dermal vessel walls in macroscopically healthy skin may be of diagnostic help in patients with chronic inflammatory connective tissue disease. | |
| 7451656 | Human histocompatibility antigen associations in subacute cutaneous lupus erythematosus. | 1981 Jan | We have identified a clinically distinct subset of lupus erythematosus patients marked by the presence of a histologically proven, nonscarring variety of cutaneous LE (subacute cutaneous LE) in which there is a very high frequency of the human leukocyte antigens (HLA) B8 and DR3. Differences in the configuration of their skin lesions allowed a separation of the patients into two clinical subgroups; annular and papulosquamous. HLA-B8 was increased in the annular subgroup (81%, corrected P (Pc) < 0.007) and combined group (65%, Pc < 0.004). HLA-DR3 was present in all 11 of the annular patients (10%, Pc < 0.00008). In addition, HLA-DR3 was present in increased frequencies in the papulosquamous subgroup (60%, Pc < 0.04) and combined group (77%, Pc < 0.00008). Thus, HLA-DR3 positive individuals have a relative risk of 10.8 for developing subacute cutaneous LE of either type and an even greater relative risk (67.1) for the annular variety. The HLA phenotype A1, B8, DR3 was also found more commonly in the annular (73%, P < 0.00008) and combined patient groups (46%, P < 0.004). These HLA associations, which are stronger than ever before reported for any form of LE, did not result from the concurrent presence of subclinical Sjögren's syndrome. Thus, subacute cutaneous LE can now be added to the growing list of HLA-B8, DR3-associated diseases that have autoimmune features. | |
| 81177 | Human triclonal anti-IgG gammopathy. II. Determination of the antigenic specificity patter | 1978 Sep | The specificity and reactivity patterns of monoclonal IgG, IgA and IgM anti-IgG autoantibodies isolated from the serum of one patient (Gil) have been determined for IgGs of the four gamma chain subclasses. The haemagglutination produced by the interaction of the Gil anti-IgGs and anti-Rh IgG coated erythrocytes was inhibited by a panel of intact IgGs, their polypeptide chains, and enzymatic fragments which included purified heavy chain constant region domains. Intact IgG1, IgG2, and IgG4 produced the same patterns of reactivity with the Gil anti-IgGs. When partially reduced and alkylated IgG1 heavy chains and its tryptic digests were tested, these were much more reactive than Fc fragments isolated from IgG of the four subclasses which were weaker inhibitors, and gamma chain constant region domains which were totally non-reactive. In all instances and by use of two anti-Rh antisera, the specificity patterns obtained for the Gil anti-IgGs were identical. The data combined with previous knowledge of the identity of the Gil light chains suggests that the antibody combining sites of these molecules are very similar if not identical. | |
| 1070338 | Oral swelling in Sjögren's syndrome. | 1976 Nov | A case is reported of a 69-year-old female with Sjögren's syndrome who presented with a problem of interest to the oral surgeon. The clinical features and cryosurgical management of a rare cause of palatal swelling are described. The confusion that exists in the literature concerning the nomenclature of chronic inflammatory salivary gland lesions is discussed. | |
| 1062746 | Sialochemistry in Sjögren's syndrome. | 1976 Feb | The value of sialochemistry in the study of Sjögren's syndrome was explored by comparative examination of a spectrum of parotid components in twelve subjects with a positive diagnosis of the disease and twelve control subjects with normal gland function. The subjects with Sjögren's syndrome all exhibited a marked reduction in flow rate and phosphate concentration and a marked elevation in sodium and chloride concentration. The concentration of IgA was somewhat elevated (commensurate with reduced flow rate); the levels of IgG, IgM, and albumin were normal. The major functional abnormality in the parotid gland in Sjögren's syndrome appears to be luminal transport in the ductal region; leakage of serum components is minimal. Sialochemistry can be helpful in differentiating Sjögren's disease from other diseases of the salivary gland and in assessing degree of pathologic change. | |
| 6508097 | The use of topical Healon tears in the management of refractory dry-eye syndrome. | 1984 Sep | We evaluated 28 patients with dry-eye syndrome in the setting of either primary or secondary Sjogren's syndrome, with topical Healon tears. In 26 of the 28 patients, we noted both subjective and objective improvement characterized by decreased itching, burning and foreign body sensation, decreased mucus stranding and Rose-Bengal staining of the cornea and conjunctiva, and increased corneal luster and tear break-up time. | |
| 6508046 | [Lymphocytic interstitial pneumonia in Gougerot-Sjögren syndrome with scleroderma]. | 1984 | The authors report the case of a 70 year old woman with a 15 year history of the Gougerot-Sjogren syndrome, Raynaud's phenomenon and sclerodactyly. Progressive respiratory symptoms in this case were not due to pulmonary fibrosis secondary to scleroderma but to diffuse interstitial lymphocytic pneumonia (ILP) secondary to the Gougerot-Sjogren syndrome. The authors emphasize the diagnostic value of the significant, permanent lymphocytosis in the aspirate after broncho-alveolar lavage. The differential diagnosis is difficult because the radiological and clinical signs of pulmonary complications of the Gougerot-Sjogren syndrome and of scleroderma are very similar. Reports of the triple association of ILP-Gougerot-Sjogren syndrome-scleroderma are uncommon in the medical literature. However, prospective studies suggest that paraclinical stigmata of the Sjogren syndrome are relatively common in patients with scleroderma. These observations suggest that some patients with clinical "pulmonary fibrosis" may in fact have ILP secondary to slowly progressive Sjogren's syndrome. | |
| 6420564 | Sjögren's syndrome with pseudolymphoma treated with chrysotherapy. | 1983 Dec | A 72-year-old woman presented with a 10 X 10 cm pseudolymphoma of the lung and Sjögren's syndrome. She was treated with weekly chrysotherapy for 4 years and had gradual diminution of the lung mass over this period along with normalization of her parotid gland scintiscan. In addition her rheumatoid factor titer (RF) of 1:160,000 fell to zero, IgM level became normal and lymphocyte mitogen response improved. On 5-year followup the patient remains stable with a negative RF, normal IgM and stable chest radiographs. | |
| 6838425 | Quantitation of tear lysozyme levels in dry-eye disorders. | 1983 Apr | A simple, rapid, reproducible method of quantifying tear lysozyme levels with a dual-channel spectrophotometer was used to compare normal subjects and those with dry-eye syndrome. The method was sensitive (80%) and specific (85%) and had a predictive value of a positive result of 83%. One patient with clinical manifestations of dry-eye syndrome and paradoxically elevated levels of tear lysozyme was found to have underlying sarcoidosis. | |
| 6296990 | [Histochemical identification of lymphocytic infiltration in the rheumatoid nodule]. | 1982 Oct | The authors have used histochemical techniques to identify lymphocytic infiltrations in the rheumatoid nodule. They used the technique of alpha-naphtyl-acetate esterase (ANAE) for macrophages, ANAE plus Sodium Fluoride (NaF) for T-lymphocytes and 5-nucleotidase for B-lymphocytes. The results of this study reveal that the lymphocytic component of the rheumatoid nodule is mostly B-lymphocytes. | |
| 350346 | Sjörgren's syndrome treated with bromhexine: a randomised clinical study. | 1978 Jun 17 | Existing treatment for Sjögren's syndrome is unsatisfactory, and uncontrolled observations have suggested that bromhexine may be effective. Twenty-nine patients with Sjögren's syndrome were therefore assigned to two randomised double-blind crossover trials with bromhexine and placebo, each comprising two two-week periods. In the first trial bromhexine 24 mg/day was given by mouth; in the second the dose was increased to 48 mg/day. After each treatment period the Schirmer test response, break-up time, Bijsterveld score, and the time taken for the patient to eat a dry biscuit were recorded, as well as the patient's estimate of moistness in the eyes and mouth. In the second (higher-dose) trial values on the Schirmer test were significantly higher after bromhexine than after placebo and the break-up time was also increased after bromhexine, which suggested that the drug has a dose-dependent effect on lacrimal gland secretion in Sjögren's syndrome. It had no effect on salivary gland function. Bromhexine is therefore valuable in the treatment of Sjögren's syndrome. | |
| 869435 | Differential diagnosis of xerostomia by quantitative salivary gland scintigraphy. | 1977 May | A new and rapid method of quantitative salivary gland scintigraphy has been developed. The uptake of pertechnetate in the glandular tissue and in a reference area in the neck are measured. The ratio between these measurements gives an index of salivary gland activity. By using this activity index, quantitative information on the functional state of the parotid and submandibular glands can be obtained. In cases of xerostomia such measurements may be particularly helpful in differentiating Sjögren's syndrome and sialosis and hence in the choice of appropriate treatment. | |
| 1064976 | [The Sjögren-Gougerot syndrome]. | 1976 Jun | The Sjögren-Gougerot syndrome represents a rare disease of the collagenous tissue characterized by the three symptoms Xerophtalmy, Xerostomy, polyarthritis. Women after the menopause are the most affected. Oral symptoms addup to diverse dermatological manifestations. The bilateral parotidic swelling and systemic complications accompany the disease, and the immunological disorder is coupled with hypergammaglobulinemia. Prognosis is very reserved and treatment delicate. |