Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 7293525 | [Distribution of immunoglobulin classes in joint capsules in osteoarthritis (arthrosis) an | 1981 Jul | The distribution of the various classes of immunoglobulins (IG) in synovial membranes in rheumatoid arthritis (RA) and in osteoarthritis (OA) is analyzed. Both plasma cells and extravascular deposits of immunoglobulins are stained using class-specific antisera and the peroxidase-antiperoxidase technique. It can be shown that the local humoral immune response in RA-Synovialitis occurs at a higher level than that of OA; inflammatory infiltrates are denser and plasma cells are activated to a higher degree which is evident in the greater intensity of cytoplasmic staining. While there is no difference in the relation between singular classes of IG in these two groups of diseases, the fraction of plasma cells synthesizing an immunoglobulin is greatly elevated in RA-Synovialitis and there is a correlation of this to the morphological degree of RA-Synovialitis. This applies even more to extravascular deposits of IG with rheumatoid-factor-activity. The determination and staining of extravascular immunoglobulins with a polyvalent anti-human-serum or with antisera specific for the gamma, alpha, and mu-antigens of heavy chains as well as the demonstration of rheumatoid-factor activity of these IG constitute a possibility to histochemically discriminate between RA-Synovialitis and OA-induced synovialitis. In OA, intensely stained areas of IG with rheumatoid-factor-activity located extravascularly are never found. | |
| 822265 | [The long term treatment of rheumatoid arthritis with niflumic acid or indometazin in doub | 1976 Jul 9 | In a double-blind study 80 patients with active rheumatoid arthritis in slightly progressed state, who had not received systemic therapy, were treated with niflumic acid and indometazin in doses of 1000 mg resp. 100 mg per day for a period of 6 months. The investigation showed good results in the patients treated with niflumic acid. In particular were observed relief of pain, shortening of morning stiffness, improvement of grip-strength, decrease of sedimentation rate. In a number of cases an assuage of the basic disease was reached which was almost equal to remission. In some aspects niflumic acid was more effective than indometazin. Of particular value are the good tolerance and the lack of sideeffects subjectively as well as objectively. Niflumic acid is a particular suitable non-steroid containing preparation for a broad range of medical applications. | |
| 808994 | Characterization of IgG complexes in patients with rheumatoid arthritis. | 1975 Jun 13 | Readily demonstrable IgG complexes are a characteristic feature of the serum and joint fluid of most patients with rheumatoid arthritis. The complexes that occur in the joint fluids have been implicated in the activation of the complement system. Studies with highly purified radiolabeled joint fluid complexes, dissociated in acid buffers, showed that isolated 7S fractions contain all the components necessary to readily reassociate and reform rapidly sedimenting complexes. Furthermore, quantitation of anti-IgG activity in the dissociated 7S fraction demonstrated that the preponderance of molecules possessed anti-IgG activity. These findings provided evidence that IgG anti-IgG molecules account for the major proportion of the precipitable IgG complexes. Quantitation by analytic ultracentrifugation revealed considerable amounts of complexes not detected by precipitation with the monoclonal anti-IgG reagents, which thus emphasizes the existence of a spectrum of IgG complexes with differing physical and biologic properties. Sodium sulfate, used at varying concentrations, proved to be useful for differentially enriching various fractions of the complexes. | |
| 6134490 | A double-blind one year clinical trial comparing alclofenac with gold in rheumatoid arthri | 1983 | Twenty-seven patients with rheumatoid arthritis entered and 20 completed a one year double blind trial in which the effects of alclofenac 1 g 3 or 4 times daily were compared with gold injections 50 mg weekly intramuscularly to 1 g then 50 mg monthly. Previous anti-inflammatory medication was continued. Assessments were done every 3 months. On both drugs there was a delayed continued improvement over at least the first 6 months in mean pain scores, morning stiffness, grip strength, articular index and an overall grading system. The mean ESR fell markedly in patients on gold, but in those on alclofenac it showed no consistent change. Several measurements showed improvements statistically compared with initial values in both groups after 6 months. Rheumatoid factor, and hand and feet X-rays, were not influenced by either treatment. Side effects were rather similar on both drugs, and 4 patients in each group developed a rash. | |
| 326444 | Rheumatoid factor plaque-forming cells in rheumatoid synovial tissue. | 1977 May | Cells producing rheumatoid factor (RF) were readily detected in vitro by means of a haemolytic plaque assay system employing sheep erythrocytes (SRBC) sensitized with reduced and alkylated rabbit IgG anti-SRBC antibody as target cells. Rheumatoid factor-producing, plaque-forming cells (RF-PFC) were observed in all of the synovial tissue cell preparations from seropositive rheumatoid arthritis patients studied. The numbers of RF-PFC varied considerably without any direct correlation with serum titres of RF antibody activity. However, high numbers of RF-PFC were never found in patients with low rheumatoid factor titres whereas, high and low numbers of RF-PFC were found among the patients with high RF titres. Synovial tissue cell preparations from a group of seronegative patients, with only one exception, failed to exhibit RF-PFC. | |
| 7391624 | [Histochemical study of lysosomal enzymes in the synovial membrane of the rheumatoid arthr | 1980 Feb | Synovial membrane obtained from the patients, in number of twenty one, suffering from the rheumatoid arthritis of classical type, was surveyed from the stand-point of enzyme histochemistry concentrating on lysosomal enzyme. Examined enzymes were leucine aminopeptidase, acid phosphatase and beta-glucuronidase. The activities of respective enzymes were compared with clinical and pathological activities of disease in each cases. It has been known that these lysosomal enzymes indicate high activity not only in chemical analysis of synovial membrane, but also histochemical observation. The origin of these enzymes, however, has not yet been determined as being derived of the synovial tissue or inflammatory cells. In the present investigation, it has been clarified that the enzyme activities were well localized at lining cell layer, but no activation of enzyme in the inflammatory cells in tissue. Therefore, it is suggested that lysosomal enzyme activity may be one of the factors which act on the destructive process in the rheumatoid arthritis. | |
| 6680663 | Antigen Dd reactivity in selected diseases. | 1983 | The distribution of detectable antibodies against antigen Dd has been studied in rheumatoid arthritis, goitre, nephrotic syndrome, cirrhosis, gastrointestinal tract diseases, neurological diseases, liver and gall bladder diseases, breast cancer, respiratory diseases and cardiovascular diseases. Except in rheumatoid arthritis, breast cancer and nephrotic syndrome, where the incidence of antigen Dd-reactivity did not differ much from that in the control group, in all other disease it was significantly lower. | |
| 518142 | Maintenance dose of penicillamine in rheumatoid arthritis: a comparison between a standard | 1979 Oct | There is much individual variation in the response of rheumatoid arthritis (RA) to penicillamine, some patients deriving benefit from very small doses. A dose of 750 mg daily is widely regarded as standard, and, while their RA commonly responds, many patients discontinue treatment because of adverse reactions to penicillamine. A more flexible prescribing policy might be more successful in the long term and was tested in 1 group of 20 patients, another receiving a 'standard' regimen, each beginning treatment at a low dose level. Of those who were given increases of dose only if response was poor 17 completed 1 year of treatment on an average maintenance dose of 308 mg daily, but only 11 of the other group on an average dose 613 mg daily. Proteinuria, which was found only in the latter group accounted for 6 withdrawals, all at doses of 625 mg daily or above. The reduction in rheumatoid activity appeared to be of about the same degree among the members of both groups who completed 12 months of treatment. Penicillamine should be given initially in a low dose and this should be raised only if there is lack of response after at least 4 weeks. | |
| 6637276 | [Indication of mycoplasmas in the synovial fluid of rheumatoid arthritis patients]. | 1983 Sep | Specimens of synovial fluid taken from patients with rheumatoid arthritis were tested for the presence of mycoplasmas and mycoplasmic antigens. In 30% of cases the direct inoculation into cell-free media permitted the detection of mycoplasma-like agents which could not be subcultured on solid media for identification. Mycoplasmic antigens were detected in the tested material with the same frequency by means of the immunofluorescence test. The use of cell cultures made it possible to isolate and identify mycoplasmas. M. arthritidis and M. fermentans, as well as their association, were identified in the immunofluorescence test and in cell cultures. | |
| 804494 | Antibodies to cellular antigens in Sjögren's syndrome. | 1975 May | An extract of human lymphocytes from continous cell culture was used as the antigen source to detect antibodies in sera of patients with Sjögren's syndrome (SS). Using double diffusion in agarose, 85 per cent of a selected group of patients had precepating antibodies. Three precipitating antigen-antibody systems were detected and were shown to be different from those described previously in othe systemic rheumatic diseases. The SS precipitating antibodies were temporarily classified as precipitins, A, B, and C. SS patinets with sicca syndrome but without clinical rheumatoid arthritis had precipitin systems A and/or B, and SS patients with associated rheumatoid arthritis had precipitin system C. Serum reactants were demonstrated by immuno-electrophoresis to migrate in the gamma globulin region. The precipitating activity of the serum factors was not destroyed by treatment with 2-mercaptoethanol and was not removed by absorption of rheumatoid factor from the sera. The reactivity of the lympuocyte antigens was destroyed by treatment with trypsin but not by deoxyribonuclease or ribonuclease. | |
| 9537052 | Immunopathologic role of proteoglycan antigens in rheumatoid joint disease. | 1980 | Cell-mediated immunity to proteoglycan antigens was assessed by leucocyte migration inhibition and by lymphocyte stimulation tests in patients with rheumatoid arthritis or with ankylosing spondylarthritis, in patients with relapsing synovitis after a single trauma to their knee joints, and in healthy donors. Both tests revealed a sensitization in most of the patients examined with various proteoglycan antigens derived from human cartilaginous tissues, rheumatoid synovial fluid, and species-common antigen of bovine nasal cartilage. Antibodies against proteoglycan antigens of human articular cartilage were detected by solid-phase radioimmunoassay in eleven out of twenty-nine sera from patients with rheumatoid arthritis and in four out of six rheumatoid synovial fluids. The results suggest that the cartilage antigenic components released by an inflammatory process or trauma may trigger a vicious circle of chronic inflammation and joint destruction. | |
| 161817 | Rheumatoid synovial lymphocytes lack concanavalin-A-activated suppressor cell activity. | 1979 | Synovial lymphocytes eluted by enzyme treatment from eleven patients with rheumatoid arthritis (RA) were investigated for the presence of concanavalin A (Con A)-activated suppressor cell activity as compared with that of peripheral blood lymphocytes of twenty normal donors. In addition, two patients with psoriatic arthritis and juvenile rheumatoid arthritis (JRA) were also investigated. Synovial lymphocytes from the eleven RA patients showed a mean augmentation of 28 +/- 13.30, and thus clearly lacked suppressor activity, whereas the mean suppression in the lymphocytes from the twenty normal donors was 13 +/- 14.40. Synovial lymphocytes from one patient with JRA and one with psoriatic arthritis showed a normal suppressor activity. | |
| 506657 | [Radiosynoviorthesis with yttrium-90-silicate. Results compared to aurum-198-colloid and w | 1979 | The results of the radiosynoviorthesis with yttrium-90 silicate in 36 joints, are reported. In comparison to the radiogold therapy in 64 joint, yttrium-90 was a little more effective. Additionally, the body distribution of radioactive yttrium after radiosynoviorthesis of knee joints, was measured in 6 patients. It could be shown that the uptake of the regional lymphnodes was between 4 and 5% of the yttrium administered. The radiation dose of the regional lymphnodes certainly exceeds 1000 rad. The results point to the importance of a careful selection of patients for radiosynoviorthesis. | |
| 4053439 | Computerized axial tomography in investigations of the rheumatoid knee. | 1985 Jul | It is essential when deciding on the therapeutic approach to the rheumatoid knee--medical or surgical--to make a precise and thorough assessment of the joint situation as a whole. In order to overcome the limitations of traditional X-ray methods, the Authors used computerized axial tomography (C.A.T.) to study the knees of twenty rheumatoid arthritis patients. They analysed three particular sections: the first at the level of the subquadriceps bursa, the second through the femoral condyles, and the third through the proximal tibio-fibular joint. C.A.T. permits an assessment of the conditions of the muscles and any hypertrophy of the synovial membrane; this kind of investigation reveals them clearly. It was possible to make an accurate investigation of the femoro-patellar joint and of lesions involving the femoral condyles and tibial plate. The findings indicate that C.A.T. in the study of the rheumatoid knee provides information on the soft tissues that cannot be obtained by traditional radiological methods. It also shows with greater precision the extent and morphology of bone lesions to the joint heads, an extremely important question in planning the use of prostheses. | |
| 7391154 | Rapid determination of indomethacin and salicylic acid in serum by means of reversed-phase | 1980 Mar 14 | A method for the quantitative analysis of indomethacin and salicylic acid in blood serum and urine by high-performance liquid chromatography is described. A C18-bonded silica was used as the stationary phase and mixtures of ethanol, n-butanol and aqueous buffer as the mobile phase. Before injection the serum is deproteinized and extracted in one step. The recovery of the extraction was found to be 88% and 77% for indomethacin and salicylic acid, respectively. The relative standard deviations of the analysis for 0.5 micrograms indomethacin and 5 micrograms salicyclic acid per ml serum were 3.6% and 3.2%, respectively. The detection limits for indomethacin and salicylic acid were 2 ng. This corresponds for both substances to 0.1 micrograms/ml serum for an injection volume of 100 microliters. The method enables simultaneous determination of possibly formed metabolites. A number of concurrently administered drugs do not interfere with the analysis. The interactive effects of co-medication of indomethacin and salicylic acid on the serum concentration of indomethacin is demonstrated by measuring the pharmacokinetic curves. | |
| 310605 | HLA-B27-positive oligarthritis. | 1978 Sep | 68 cases with polyarthritis were selected from 406 HLA B 27 positive patients with various rheumatic diseases excluding ankylosing spondylitis (AS) or Reiter's disease. 23 fulfilled at least 5 criteria of the ARA for the diagnosis of rheumatoid arthritis (RA). 5 suffered from polyarthritis and psoriasis. The remaining 40 patients expressed an asymmetric oligarthritis especially of the lower limbs (knee, ankle) affecting predominantly young adult men. Sacroiliitis was observed in 10 cases. Joint erosions, rheumatoid factors and visceral manifestations were uncommon. The arthritic pattern of B 27 positive oligarthritis differed clearly from rheumatoid arthritis (n = 34) and psoriatic arthritis (n = 15), but was similar to peripheral joint involvement in AS (n = 32) except for the higher incidence of coxitis in AS. HLA typing is helpful not only in the early diagnosis of AS but also in the differential diagnosis of unclassifiable polyarthritis. | |
| 7458434 | Cell-mediated cytotoxicity for cultured autologous rheumatoid synovial membrane cells. | 1980 Dec | Cytotoxicity tests were performed on cultured rheumatoid synovial membrane cells with autologous lymphocytes as effector cells. Three of 26 cultures were positive when tested at primary or first passage level, but on subsequent tests became negative. Tests based on an isotope-labelling technique and a micro-cytotoxicity assay were compared; the results appeared to correlate well. The expression of neoantigens on synovial cells was not demonstrated. Cultures initiated in medium containing hydrocortisone did not give rise to a population of synovial cells susceptible to immune lysis by autologous lymphocytes. | |
| 1084547 | Suppression and stimulation of immunoglobulin synthesis by agents present in rheumatoid sy | 1976 May | Synovial effusions from 14 patients with rheumatoid arthritis contain factors capable of enhancing and suppressing the production of immunoglobulin when added to normal peripheral lymphocyte cultures in vitro. Utilizing a radioactive immune coprecipitation technique it could be demonstrated that enhancement was observed upon addition of relatively small amounts of synovial fluid, while addition of higher amounts resulted in suppression of immunoglobulin synthesis. Nine of ten non-rheumatoid joint effusions showed neither enhancing nor suppressing activities. The possible relationship of these events to lymphokine production in the joint is discussed. | |
| 327037 | Characteristics of mononuclear cell populations in chronically inflamed synovial membranes | 1977 Jan | Mononuclear cells infiltrating synovial membranes in chronic synovitis were characterised both in situ and in cell suspensions by surface markers and histochemical techniques. T-lymphocytes were the predominant infiltrating cell in rheumatoid arthritis as well as in other forms of chronic arthritis, including ankylosing spondylitis and arthritis associated with Crohn's disease. B-lymphocytes were found exclusively in rheumatoid synovial membranes. These cells were demonstrable both in true germinal centres and, focally and diffusely, in nodular mononuclear infiltrates lacking the histochemical characteristics of germinal centres. The synovial lining cells, unlike mononuclear phagocytes, had no demonstrable receptors for C3 and Fc. | |
| 567304 | Penicillamine-induced myasthenia gravis associated with antibodies to acetylcholine recept | 1978 Aug | A woman with rheumatoid arthritis was treated with penicillamine and developed myasthenia gravis. This drug-induced disease was associated with characteristic autoantibodies to acetycholine receptor. After discontinuing the drug, her symptoms improved and the antibody titers fell. Penicillamine is now being used much more frequently in the treatment of rheumatoid arthritis and it is likely that this complication will become more prevalent. |