Search for: rheumatoid arthritis methotrexate autoimmune disease biomarker gene expression GWAS HLA genes non-HLA genes
| ID | PMID | Title | PublicationDate | abstract |
|---|---|---|---|---|
| 7103708 | [Clinical aspects of the radioimmunological determination of serum ferritin]. | 1982 Jan | Personal experience confirms the diagnostic value of serum ferritin estimation in the iron deficient microcytic anemia, where there are low levels. The clinical significance of the test is restricted by the not negligible incidence of levels like that of iron overload in the hepatic and neoplastic diseases. In hepatic diseases the test resulted to be related to the changes of serum transaminases. In malignant neoplasms the high serum ferritin levels had been only sometimes associated with transfusional siderosis. | |
| 285374 | Vasculitis in a patient receiving naproxen. | 1979 Jan 24 | A case is described of a 44-years-old woman who developed cutaneous vasculitis, nephritis and paralytic ileus after three years' treatment with naproxen. All these features were thought to be attributable to a naproxen induced vasculitis and all resolved spontaneously after the drug was stopped. | |
| 6602706 | Multicentre trial of piroxicam in ambulant rheumatology. | 1983 | A total of 8299 patients suffering from rheumatoid arthritis, osteoarthrosis, ankylosing spondylitis or non-articular rheumatism were included in a large non-comparative multicentre study. In all, 98% of the patients reported some functional disability at baseline. At the final visit, only 20% had more pronounced incapacity. Pain, as assessed by the physician, was reduced by 44%. Overall, 70% of the patients having had recent treatment with a NSAID (n = 5489) rated current piroxicam treatment as better. The toleration of the piroxicam treatment was good. A total of 17% of the patients reported side effects, mainly from the gastrointestinal tract. Gastroduodenal ulcers were reported by 5 patients (0.06%). | |
| 506490 | Chronic synovitis with marked infiltration of granulocytes in a patient with an unusual de | 1979 Jul | The case described concerns a patient initially presenting the clinical symptoms of rheumatoid arthritis, including the presence of nodules, and slowly developing into systemic lupus erythematosus (SLE). Besides the signs of chronic inflammation in the synovial tissue, many granulocytes and PAS-positive macrophages were present. At electronmicroscopy the basal membranes of the vessels were multilaminated and the endothelial cells enlarged while many macrophages contained large clumps of electrondense material which could easily be interpreted as rough endoplasmatic reticulum (RER) of plasma cells. Immunoflourescence studies showed deposition of immunoglobulins and complement in the vessel walls of the synovium, thus suggesting an immune-complex pathogenesis. | |
| 6333007 | Use of the HLA B27 test in Auckland. | 1984 Oct 10 | An assessment was made of the probability of HLA B27 associated disease in 74 patients who had recently been tested. Six patients had definite, 31 possible, and 37 very unlikely HLA B27 associated disease. Thirteen patients were HLA B27 positive. Fourteen tests were ordered by rheumatologists (43% HLA B27 + ve) and 60 by other doctors (12% HLA B27 + ve) p less than 0.01. Non-rheumatologists ordered an excessive number of tests in patients with a low probability of HLA B27 associated disease. | |
| 786850 | Human complementary component C'3: an appraisal. | 1976 Aug 30 | Results obtained so far on the C3 polymorphism suggest that the system should be a valuable marker in population studies. The instability of the complement component C3 may, however, cause some practical problems in population genetic fieldwork, since a certain fraction of serum samples may be difficult to type with certainity due to storage alterations. Studies of the conversion rate and the concentration of C3 have shown that there is no significant difference between the phenotypes. And furthermore, the fact that there is good agreement with the Hardy-Weinberg distribution indicates that the conversion has had no appreciable selective effect on the phenotype distribution (Brönnestam et al., 1971). | |
| 4006302 | Studies on fibronectin in inflammatory vs non-inflammatory polymorphonuclear leucocytes of | 1985 May | We conducted studies dealing with the synthesis and release of fibronectin in vitro by polymorphonuclear leucocytes (PMNL). The specific purpose of our study was to look for any changes in these events as they happen in inflammatory vs non-inflammatory PMNL. We used PMNL isolated from the synovial fluid of patients with rheumatoid arthritis as a source of inflammatory cells and PMNL isolated from peripheral blood as a source of non-inflammatory cells. Marked differences were observed. Using 35S-methionine metabolic labelling and SDS-polyacrylamide gel analysis, we were first able to clearly observe an increased synthesis of fibronectin by inflammatory PMNL when compared to non-inflammatory PMNL. Furthermore, the release of fibronectin in vitro by these cells was increased by factors of up to 20 when compared to non-inflammatory peripheral blood PMNL. Experimental evidence was also obtained which strongly suggests that fibronectin exists in a stored form inside the inflammatory PMNL we used in this study. Finally, we observed that PMNL are capable of synthesizing a 95 kD gelatin binding protein which appears to be distinct from fibronectin. | |
| 219466 | Relationship between life stress factors and viral antibody levels in patients with juveni | 1979 | Serum specimens from 46 patients with JRA were tested for measles CF, HI, HLI, and RNP-GP antibodies, for rubella HI antibodies, and for adenovirus and herpes simplex virus CF antibodies. The mean antibody titres of the 16 JRA patients of the major conflict group (14) were generally higher than those of the 30 patients of the non-conflict group, although the differences do not reach the level of statistical significance. The interrelationships between life stress, emotions and immunological changes in JRA are discussed. | |
| 7005120 | Identification and functional characterization of monocytes in rheumatoid synovial fluid. | 1981 | Mononuclear cell suspension from synovial fluids of 13 patients with rheumatoid arthritis and 5 patients with juvenile rheumatoid arthritis contained on average of 1.5 and 1.3% cytotoxic plaque-forming cells, respectively, when tested against a monolayer of antibody-sensitized sheep erythrocytes. The plaque-forming ability was completely abolished after removal of cells which had phagocytosed carbonyl iron. The highest proportion of plaque-forming cells was found in the cell suspensions that contained the highest percentage of peroxidase-positive mononuclear cells. The plaque-forming activity was almost completely inhibited by human IgG. Cell suspensions from patients treated with corticosteroids contained the highest proportions of plaque-forming cells and peroxidase-positive mononuclear cells compared with patients not receiving such treatment. Our findings indicate that the plaque-forming leukocytes in rheumatoid synovial fluid are monocytes. | |
| 701459 | Determination of human immunoglobulin M rheumatoid factor by a solid-phase radioimmunoassa | 1978 Aug | A solid-phase radioimmunoassay for the rapid determination of human immunoglobulin M (IgM) rheumatoid factor (RF) has been developed. Preparation of the solid phase for the assay involved the formation of complexes between respiratory syncytial virus-specific human IgG antibodies and virus antigen on the surface of polystyrene balls. Binding of serum RF to IgG in the immune complex was subsequently detected by 125I-labeled mu-chain-specific antibodies to human IgM. The amount of radioactive indicator antibody bound was converted to units of RF by comparison to the standard curve for an RF reference-serum pool. This assay should prove useful in studies of the physiological role of RF, since it can effectively measure low levels of circulating RF. | |
| 7379397 | The synovial membrane in human coxathrosis: light and electron microscopic studies. | 1980 May | Changes of the synovium are in integral part of osteoarthritis. Most authors regard these changes as secondary to cartilage degeneration. However, synovitis is a very early feature in osteoarthritis, and increased knowledge of changes in the chemical composition of the synovia focused our attention on the histologic characteristics of osteoarthritic synovium. Light and electron microscopic studies of the synovial membrane from patients with osteoarthritis and rheumatoid arthritis of the hip joints were performed. Two distinct types of osteoarthritic synovitis were observed: an early proliferative from characterized by venous stasis with edema, free erythrocytes and hemosiderine deposits in the interstitial tissue, that suggested increased capillary permeability. In the late form, fibrous synovitis, the microscopic picture was dominated by dense fibrous tissue. Synovium from rheumatoid arthritis showed the same vascular changes as in osteoarthritis. In addition, the histologic picture was characterized by severe inflammatory changes. In osteoarthritis the signs of inflammation were moderate or absent. The development from proliferative into fibrous synovitis is probably the result of long-standing chronic venous insufficiency. The essential feature of osteoarthritic proliferative synovitis--venous stasis with increased capillary permeability--correlates well with most of the known changes in the composition of the synovial fluid. | |
| 3936928 | The presence and possible significance of C-reactive protein in rheumatoid inflammation. | 1985 Oct | C-reactive protein (CRP) is capable of binding to a variety of ligands with subsequent complement fixation in vitro. By this means CRP may contribute to the inflammatory process in some diseases. Elevated levels of CRP were demonstrated in synovial fluids (SF) from patients with a variety of inflammatory arthritides. The contribution of CRP to inflammation in SF was investigated. Sera and SF from patients with rheumatoid and other forms of arthritis were examined for the presence of ligand bound CRP. We were unable to demonstrate significant amounts of bound CRP, either as increased molecular weight using gel filtration, or in the form of CRP-ligand-immunoglobulin complexes using a modified enzyme linked immunosorbent assay. CRP was found associated with the pellet after centrifugation of SF samples. Preliminary studies showed CRP detectable on the surface of cells in some inflammatory SF. The presence of this cell associated CRP suggests that ligand bound CRP may not be demonstrable in the sera and SF examined due to interaction with cells. | |
| 6492692 | Differential generation of chemiluminescence--detectable oxygen radicals by normal polymor | 1984 Aug 1 | The generation of chemiluminescence (CL)-detectable oxygen radicals by normal human polymorphonuclear leukocytes (PMN) after challenging with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) sera is described. CL was measured in a luminol-dependent assay and referred to a standard obtained when performed immune complexes (Ic) (human tetanus toxoid-antitoxoid Ic resuspended in normal pooled serum) were tested on PMN. Normal sera gave rise to CL activity by PMN between 0% and 50% of the standard Ic (mean +/- standard error of the mean (SEM): 20.7 +/- 4.8). Sera from SLE and RA patients induced strikingly different biological effects on PMN. SLE sera generally induced a high CL-detectable generation of oxygen metabolites which may be causally related to the intense tissue damage (vasculitis) frequently observed in this disease. In contrast to SLE, RA sera induced a CL-detectable respiratory burst by PMN that was included in the normal range. Thus, the biological effects of these sera in terms of stimulation of toxic oxygen radical generation by phagocytes are quite different. This generation of oxygen radicals might reflect a different clearance of circulating Ic by PMN in SLE and RA disease. | |
| 6512376 | Replacement of destroyed metacarpal heads by autografting metatarsal heads. | 1984 Oct | A surgical procedure of replacing a destroyed metacarpal head with a metatarsal graft is described. With this method internal fixation with foreign material as well as postoperative immobilisation can be avoided. A follow up of 25 autotransplantations in rheumatoid patients is presented, showing no complications and fair function of the new operated joint. | |
| 535227 | Popliteal cyst mimicking thrombophlebitis as the first indication of knee disease. | 1979 Oct | Five patients, none of whom were previously known to have knee disease, developed leg swelling which was misdiagnosed as thrombophlebitis. Four were treated with anticoagulants and one developed a large calf hematoma requiring fasciotomy. The primary physicians failed to appreciate evidence of knee disease in these patients, although the rheumatology consultant found knee effusion, and a popliteal cyst was found by arthrography or ultrasonography in every case. The occurrence of popliteal cyst as the first sign of knee disease emphasizes the importance of careful examination of the knee in all patients with a clinical picture of thrombophlebitis. | |
| 7293526 | [Course of enzymopenic hemolytic anemia (glutathione reductase deficiency) simulating chro | 1981 Jul | Enzymopenic haemolytic anaemias are rare diseases in which there is an excess iron turnover. The iron is mainly deposited in many tissues giving rise to fibrosis and tissue damage. There is always a specific arthropathy; by involving a number of joints at the same time the clinical picture may resemble active rheumatoid arthritis, and therefore an erroneous treatment with gold, chloroquine, D-penicillamine or corticosteroids is possible. The diagnostic procedure in a patient suffering from enzymopenic haemolytic anaemia and pathogenetic mechanisms are discussed. | |
| 582857 | Herpes zoster and lower motor neurone paresis. | 1979 Aug | Fifteen cases of herpes zoster with lower motor neurone paresis involving the upper and lower limbs are reviewed. Five patients had an underlying disease--three had rheumatoid arthritis, two of whom were on prednisolone; one had chronic lymphatic leukaemia and one lymphosarcoma. Details are given of the time relationship between onset of pain, the appearance of the skin eruption and the later muscle weakness. Electromyographic evidence was available in 12 patients. The difficulty of assessing the muscle power in the presence of severe pain is discussed. Prognosis was generally very good; 11 patients recovered fully, three improved and one was unchanged after 5 months, when he died of lymphosarcoma. One patient was lost to follow-up at 5 months but was improving at the time. | |
| 417730 | Synthesis by an established lymphocyte cell line from a rheumatoid synovium. | 1978 Apr | Lymphocytes derived from the synovium of a patient with rheumatoid arthritis were shown to synthesize predominantly IgG as measured by an equilibrium binding assay. Chromatographic separation of the radiolabeled lymphocyte culture supernatant revealed rheumatoid factor activity associated with IgG and IgM. In addition, immunofluorescence studies on fixed lymphocytes demonstrated that the majority of cells stained positive for both IgG and rheumatoid factor. | |
| 6610915 | Lymphocyte subpopulations in rheumatoid arthritis. An immunological, enzyme histochemical | 1984 | Peripheral blood lymphocytes (PBL) and synovial fluid lymphocytes ( SFL ) of patients with rheumatoid arthritis (RA) were examined with monoclonal antibodies, with coated ox red blood cells for the expression of Fc receptors for IgG or IgM (T mu and T gamma cells), and incubated for the demonstration of alpha- naphtyl acetate esterase and acid phosphatase. Equal percentages of OKT4 and OKT8 PBL were found in clinically active and inactive RA patients, and in healthy controls, but decreased percentages of OKT4 and increased percentages of OKT8-positive lymphocytes were found among the SFL . The percentages of T mu and T gamma cells, the presence of HLA-DR membrane antigens on T lymphocytes as well as the staining pattern for the enzymes revealed that SFL of patients with RA were highly activated, compared to PBL of RA patients and healthy controls. It can be concluded from this study that a single determination of OKT4 and OKT8-positive lymphocytes in the peripheral blood of RA patients has no predictive value for disease activity. However, the results of the experiments on T lymphocyte-activation clearly showed preferential activation of SFL compared to PBL, indicating that activation of lymphocytes occurs at the site of inflammation. | |
| 7163733 | Intra-articular drug therapy in rheumatoid arthritis. A study with indoprofen. | 1982 | Six patients (4 men, 2 women) with moderate/large knee effusions due to rheumatoid arthritis (RA) were studied after receiving indoprofen, 25 mg intra-articularly and then 200 mg orally 1 week later. There was significant improvement in pain (t = 3.74, P less than 0.05), morning stiffness (t = 2.91, P less than 0.05) and range of movement (t = 2.52, P less than 0.05) for at least 1 week following the intra-articular injection. The terminal phase plasma half-life after the 200 mg oral dose was 6.4 +/- 0.7 h (mean +/- SEM) and was significantly longer than the often quoted plasma half-life of 2-3 h from previous studies, but much less than the pharmacodynamic half-life. Synovial fluid concentration were not significantly different from those in plasma in the post-distribution phase. Intra-articular indoprofen may be a useful addition to the treatment of RA. |